• Korean Journal Plant Pathology
• /
• v.13 no.1
• /
• pp.57-62
• /
• 1997

This experiment was conducted to test the control effect of methanol extracts of 10 medicinal plants on apple storage diseases caused by Botryosphaeria berengeriana, Glomerella cingulata and Penicillium expansum. Out of the 10 medicinal plants, methanol extracts of Coptis japonica and Anemarrhena asphodeloides inhibited effectively the mycelial growth of B. berengeriana, G. cingulata and P. expansum in vitro, for which the inhibition ratios of the two plant extracts were 100.0% and 89.3%, 73.7% and 94.1%, and 100.0% and 51.6%, respectively. Spore germination of the three fungi was inhibited 100% only by C. japonica extract, but only P. expansum was inhibited 100% by A. asphodeloides extract. No lesion was formed y the fungi at 5$^{\circ}C$ up to 2 weeks after inoculation. Lesion sizes produced by the three fungi at the temperature ranges of 1$0^{\circ}C$ to $25^{\circ}C$ and infection of B. berengeriana and G. cingulata were inhibited by C. japonica extract, but not by A. asphodeloides extract, while no lesion was formed by the fungi at 5$^{\circ}C$. Infections of the fungi on apples were somewhat stimulated by A. asphodeloides extract.

• Journal of Animal Science and Technology
• /
• v.50 no.6
• /
• pp.831-838
• /
• 2008

This study was conducted to evaluate effects of mixed microbes addition on physico-chemical, fermentative and microbial parameters of sawdust-based spent mushroom substrates(SMS). The SMS was inoculated with mixed microbes(Enterobacter ludwigii, Bacillus cereus, 2 strains of Bacillus subtillis, Saccharomyces cerevisiae and Lactobacillus plantarum) at 1% level(wet basis) and anaerobically fermented during the different periods(up to 8th week). Compared with the SMS before ensiling, the ensiled one had higher CP, NDF and ADF percentages and lower DM and NFC percentages. However, levels of change were very low. The in situ ruminal disappearance of SMS DM and NDF decreased with the ensiling period prolonged. For fermentative parameters, pH reduced and lactic acid contents increased after ensiling, compared with those after ensiling. At 8th week of ensiling, pH increased and lactic acid contents reduced again, compared with those at 4th week of ensiling; however, the silage still showed favorable fermentation status. Lactic acid bacteria counts did not change throughout 8 weeks of ensiling. Counts of total microbes and yeast reduced after 4th week of ensiling period. Counts of lactic acid bacteria and yeast at 8th week of ensiling were in the levels of 108cfu/g. These results indicate that anaerobic fermentation with microbial addition could be an effective way for the long term(8 weeks) storage of the SMS.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
• /
• v.6 no.1
• /
• pp.31-36
• /
• 2000

180g of fermented soy paste and 150g of red pepper paste were packaged in glass jars of 232 mL with different conditions of active packaging and then stored at $13^{\circ}C$ for about 170 and 128 days, respectively. During the storage, package atmosphere, surface color, pH, acidity and microbial flora were monitored to see the effect of packaging conditions. Test packaging conditions include package equipped with $Ca(OH)_2\;as\;CO_2$ absorber, package with pinhole and closed control one. Closed control packages of soypaste and red pepper paste showed the increased $CO_2$ partial pressure, the decreased $O_2$ partial pressure and the constant $N_2$ partial pressure to produce high pressure buildup with storage. The paste packages with $Ca(OH)_2$ maintained relatively low $CO_2$ partial pressure and thus the package pressure close to normal atmospheric pressure for initial storage period of 70 days. The packages with air pinhole channel had the partial pressures of $O_2\;and\;N_2$ decreased with storage time, while $CO_2$ partial pressure first increased to a maximum and then slowly decreased thereafter Without any pressure increase the packages with pinhole gave the lowest quality changes possibly due to the effect of package atmosphere, but it had problem of mold contamination and growth for soy paste after 120 days. There were no difference in microbial flora between the packages after about 70 day storage.

• Korean journal of applied entomology
• /
• v.53 no.2
• /
• pp.157-169
• /
• 2014

Between 2010 and 2012, diseases and insect pests of sweet persimmon were surveyed at sweet persimmon export complexes and non-export orchards in Suncheon, Jeonnam Province; Jinju, Changwon (Dongeup and Bukmyeon), and Gimhae, Gyeongnam Province; and Ulzu, Ulsan. The following diseases were found in the sweet persimmon orchards: angular leaf spot (Cercospora kaki), anthracnose (Colletotrichum gloeosporioides and Colletotrichum acutatum), circular leaf spot (Mycosphaerella nawae), powdery mildew (Phyllactinia kakicola), and gray mold (Botrytis cinerea). Circular leaf spot was the most frequent and serious disease, and C. gloeosporioides and C. acutatum were found on fruits. Thirty-three insect pest species that belonged to 32 genera of 20 families in 5 orders were found in the sweet persimmon orchards; the two-spotted spider mite, Tetranychus urticae, was also found in the surveyed orchards. Apolygus spinolae, Pseudaulacaspis cockerelli, and Adoxophyes orana were widely found in the surveyed orchards; Spodoptera litura and Homona magnanima were also recorded. Damage by insect pests was low, and the quarantine insect pests peach pyralid moth (Dichocrocis punctiferalis) and persimmon fruit moth (Stathmopoda masinissa) were rarely or not found in the sweet persimmon export complexes. In addition, other quarantine insect pests, such as persimmon false spider mite (Tenuipalpus zhizhilashviliae) and Japanese mealybug (Planococcus kraunhiae), were not detected. These quarantine insect pests were also not found in the sorting places, storage houses, and fruits for export; however, scale insects and two-spotted spider mites were found at a low rate. Although anthracnose (C. acutatum) infested fruit was found in the storage houses, only one in Jinju and Gimhae.

• Research in Plant Disease
• /
• v.10 no.3
• /
• pp.188-193
• /
• 2004

The symptom expressions such as yellowish and mosaic spots in overwintering barley have been considered to be a damage by cold or water. However, it had revealed that the symptom expressions were caused by viruses throughout three year nationwide surveys. Barley yellow mosaic virus (BaYMV), Barley mild mosaic virus (BaMMV), and Soil-borne wheat mosaic virus (SBWMV) was detected in 2001-2003 and Barley yellow dwarf virus-MA V (BYDV -MA V) from field samples collected on March in 2003. The results of investigation showed that the incidence of BaYMV was more than 70％ and that of BaMMV and SBWMV was 15.7-37.4％ and 0.7-10.1 ％ in three year surveys, respectively. The incidence of BYDV-MAV was approximately 1 ％ in 2003 only. The distribution of BaYMV was relatively uniform throughout barley fields in Korea, but the incidence of the virus in Gyunggi Province was as low as 19％ compared to 65-85％ in the rest of regions. On the other hand, 70％ of BaMMV was found to be in the west south regions of Korea, Jeonbuk and Jeonnam Provinces. Taken together, both BaYMV and BaMMV were thought to be dominant casual agents in overwintering barley by either single or mixed infections. Previous survey data for BaYMV from 1994 to 1996 indicated that the incidence of the virus was approximately 40％ in Jeonbuk, Jeonnam, and Gyungnam Provinces. Thus, comparing with the results from the recent nationwide survey, the incidence of BaYMV had been rapidly increasing in overwintering barley fields in the southern part of Korea.

• The Korean Journal of Pesticide Science
• /
• v.13 no.3
• /
• pp.148-158
• /
• 2009

We tested and selected some agrochemicals reducing the occurrence of major pests and diseases during garlic storage. Tebuconazole, diphenylamine and prochloraz as fungicides and dimethate as a insecticide were sprayed or drenched before harvest. And the harvested garlic was dipped in each of the agrochemicals. The residues of pesticides in garlic bulbs treated were analyzed every month from harvesting time for 6 months. In case of Danyang garlic, which was treated with pesticides before and after harvesting, the residues of diphenylamine, tebuconazole, prochloraz, and dimethoate ranged from 0.008 to 0.28, from 0.03 to 0.32, from 0.02 to 0.12, and from 0.02 to 0.25 mg/kg, respectively. In case of Uiseong garlic, the residues of diphenylamine, tebuconazole, prochloraz and dimethoate ranged from 0.008 to 0.09, from 0.08 to 0.45, from 0.02 to 0.57, and from 0.04 to 0.38 mg/kg, respectively. And, in case of Namdo garlic, the residues of diphenylamine, tebuconazole, prochloraz, and dimethoate ranged from 0.008 to 0.52, from 0.07 to 1.67, from 0.02 to 0.17, and from 0.03 to 0.73 mg/kg, respectively. Some of the garlic samples treated with tebuconazole exceeded its maximum residue limits (MRLs) of 0.1 mg/kg set by Korea Food Drug Administration (KFDA), but dimethoate was detected below its MRL of 1.0 mg/kg. In case of diphenylamine and prochloraz, their MRLs for garlic were not set. Adapting their MRLs, 5.0 mg/kg of diphenylamine for apple and pear and 0.5 mg/kg of prochloraz for strawberry and grape, residue levels of diphenylamine and procloraz were below than their MRLs, with the exception of samples two times treated with procloraz in Namdo garlic. These results indicate that dimethoate can be used as an agrochemical to control the postharvest disease in garlic in only MRL aspect.

• Food Engineering Progress
• /
• v.21 no.1
• /
• pp.29-35
• /
• 2017

Quality changes of dried sea cucumber (Stichopusjaponicus) after applying a cyclic rehydration and retorting process were investigated during 4 weeks of storage. The length, volume, and weight of dried sea cucumber increased significantly as the number of rehydration cycles increased. Sea cucumber (SC) was bottled in the glass jar and a retort thermal process ($121.1^{\circ}C$, 0.15 MPa) was applied. The total thermal processing time (TTT) was 24 min based on the temperature at the cold point. The size and texture of retorted SC were significantly changed until the first week of storage. However, regardless of the number of rehydration cycles, the size and texture of samples at different rehydration cycles showed no significant difference during the whole storage. The length, volume, weight, rehydration ratio ($R_R$), hardness, and chewiness at the maximum degree of swelling during rehydration of dried SC were estimated as 100.86 mm, 38.62 mL, 41.05 g, 6.39 of $R_R$, $249.19g_f$, and 4.05 mJ, respectively.

• Research in Plant Disease
• /
• v.22 no.2
• /
• pp.94-99
• /
• 2016

For the purpose of practical application of gamma irradiation for export lily without the ionic energy damage, 150 Gy gamma ray was suggested as the safe level on cut lilies, the oriental cultivar Siberia (white) and Sorbonne (pink). The combined treatments of 150 Gy gamma ray and sodium dichloroisocyanurate (NaDCC) at 0, 40, 100, and $200{\mu}g/l$, were tried to control lily leaf blight caused by Botrytis elliptica. Incidences of petal blight on the two oriental lilies at control were 93%-95%, whereas those at the combined treatments of 150 Gy and $100{\mu}g/l$ or higher of NaDCC were 76%-83%. However, severity of petal blight was not reduced by the combined treatments. Gamma irradiation at 150 Gy did not change the cut lily quality such as improve cut lily longevity of vase-life compare with the control. In addition, fresh weight, chlorophyll contents, and the color of pink petals of Sorbonne were not changed by 150 Gy of gamma irradiation. Therefore, we recommend the combined treatment of 150 Gy gamma irradiation and $100{\mu}g/l$ NaDCC for the standard operating procedure on cut lily for export quarantine to control leaf blight.

• Research in Plant Disease
• /
• v.16 no.3
• /
• pp.316-319
• /
• 2010

The gray mold disease occurred on tuberous roots of yacon in storage facilities in Gangneung, Korea, in March 2010. Symptoms typically appeared as in the form of dark brown discoloration on the surface of tuberous roots and water-soaked brown lesions in cross sections of the affected portions. A total of five isolates of Botrytis sp. were obtained from the symptomatic portions. All isolates on potato-dextrose agar (PDA) produced abundant conidia which were pale brown, one-celled, mostly ellipsoid or ovoid in shape and $8.2{\sim}14.8{\times}6.5{\sim}9.9\;{\mu}m$ in size. Large numbers of round to irregular, smooth, black, hard sclerotia were produced on PDA over time. The optimal temperature for mycelial growth and sclerotia formation of the fungal isolates was $20^{\circ}C$. On the basis of morphological and cultural characteristics, all the fungal isolates were identified as Botrytis cinerea. Pathogenicity test on host plants showed that the fungus could infect not only tuberous roots but also leaves and petioles of yacon. This is the first report on gray mold of yacon (Smallanthus sonchifolius) caused by Botrytis cinerea in Korea.

• Journal of Sericultural and Entomological Science
• /
• v.51 no.1
• /
• pp.36-40
• /
• 2013

To promote the consumption of mulberry fruit, we manufactured mulberry fruit wine with 'simheung' by different processing methods and analyzed cyanidin-3-glucoside(C3G) stability and antioxidant capacity. In the processing of mulberry fruit alcoholic drink, 3 minutes blanching using microwave inhibited the C3G destruction by maintaining the antioxidant capacity and inactivation of enzymes related to pigment's stability. And freeze-dried mulberry fruit was the highest C3G pigment content and antioxidant capacity. Nevertheless, this is not recommended because the economic burden of the cost of freeze-drying. In conclusion, when processing mulberry fruit wine, the addition of citric acid and the enzyme's inactivation treatment were considered more effective than storage containers (transparent glass, brown glass bottles, aluminum foil, green glass, translucent glass bottles).