• Proceedings of the Korean Information Science Society Conference
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• 2005.07b
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• pp.10-12
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• 2005

웹 서비스는 소프트웨어 컴포넌트의 재사용 및 상호 운용을 지원하여 서비스의 통합을 가능케 하는 기술이다. 웹 서비스 기술을 이용하여 기존에 존재하는 서비스를 통합하여 새로운 서비스를 제공해주는 것이 가능하다. 서비스 조합을 위해 정확한 서비스 탐색은 필수적이다. 본 논문에서는 서비스 조합을 효율적으로 제공하기 위해 오퍼레이션 중심의 시맨틱 웹 서비스 탐색 방법을 제안한다.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2008.04a
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• pp.288-291
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• 2008

본 논문은 실수 진화 알고리즘에 대해서 가상의 클러스터를 이용한 재조합 연산자 및 새로운 세대차 모델을 소개한다. 가상 클러스터의 자가 적응적인 크기 변화를 통해 자손의 생성범위를 적절히 조절하고, 선택과 대치를 포함한 진화방식을 개선하여 효율적인 세대차 크기를 구함으로서, 개체의 다양성 유지 및 탐색성능의 향상을 꾀하였다. 제안된 방법을 벤치마크 테스트 문제에 적용하여 G3 알고리즘과 CMA-ES 등과 성능을 비교하였다.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.711-714
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• 2001

The production of the recombinant Plasmodium vivax merozoite surface protein (PvMSP) has been investigated in the recombinant E.coli system. Experimental optimization of the culture conditions, such as the effect of initial pH, and operating temperature has been tried on the growth of recombinant E.coli and on the overproduction of the target foreign protein.

• Journal of Intelligence and Information Systems
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• v.3 no.2
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• pp.1-9
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• 1997

These papers propose an evolutionary algorithm for re-using output of waste fuel of light water reactor system in nuclear power plants. Evolutionary algorithm is useful for optimization of the large space problem. The wastes contain several re-useable elements, and they should be carefully selected and blended to satisfy requirements as input material to the heavy water nuclear reactor system. This problem belongs to a NP-hard like the 0/1 Knapsack problem. Two evolutionary strategies are used as a, pp.oximation algorithms in the highly constrained combinatorial optimization problem. One is the traditional strategy, using random operator with evaluation function, and the other is heuristic based search that uses the vector operator reducing between goal and current status. We also show the method, which performs the feasible teat and solution evaluation by using the vectorized data in problem. Finally, We compare the simulation results of using random operator and vector operator for such combinatorial optimization problems.

• KSBB Journal
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• v.19 no.4
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• pp.331-334
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• 2004

In vivo characterization of FadB homologous enzymes including PaaG, YdbU and YgfG for medium-chain-length (MCL) polyhydroxyalkanoate (PHA) biosynthesis was carried out in fadB mutant Escherichia coli. Previously, it was reported that amplification of FadB homologous enzymes such as PaaG and YdbU in fadB mutant E. coli resulted in enhanced biosynthesis of MCL-PHA by greater than two fold compared with control strain. In this study, we constructed paaG fadB double mutant E. coli WB114 and ydbU fadB double mutant E. coli WB115 to investigate the roles of PaaG and YdbU in biosynthesis of MCL-PHA. Inactivation of paaG and ydbU genes in fadB mutant E. coli harboring Pseudomonas sp. 61-3 phaC2 gene reduced the MCL-PHA production to 0.16 and 0.16 PHA g/L, respectively from 2 g/L of sodium decanoate, which are much lower than 0.43 PHA g/L obtained with fadB mutant E. coli WB101 harboring the phaC2 gene. Also, we identified new FadB homologous enzyme YgfG, and examined its roles by overexpression of ygfG and construction of ygfG fadB double mutant E. coli WB113.

• The Korean Journal of Mycology
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• v.25 no.4 s.83
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• pp.362-368
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• 1997

Five formae speciales of Fusarium oxysporum in Korea were examined using RFLP analysis to find the possibility for classification and analyze genetic variations. DNAs from F. oxysporum f. sp. lycopersici, cucumerinum, fragariae, garlic and sesami were used with three recombinant probes such as pFC46, pFC52 and pFC57. Distinct differences among five formae speciales of this fungus were detected in RFLP band patterns based on southern hybridization of genomic DNA using each recombinant clone, which was a repetitive copy probe. Strains belong to four formae speciales could be very stable in genetic variation except f. sp. sesami which has more variation than the others based on the RFLP analysis. They formed their own cluster which has high similarity within the same formae specialis resulted from the UPGMA analysis for genetic relationship analysis and each cluster represented its own formae specialis. The method using three recombinant DNA probes could be a good tool for classification of formae speciales in F. oxysporum.

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.176-176
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• 2019

• Proceedings of the Korean Society of Crop Science Conference
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• 2020.06a
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• pp.162-162
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• 2020

• Tuberculosis and Respiratory Diseases
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• v.61 no.3
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• pp.239-247
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• 2006

Background: Culture filtrate proteins secreted by mycobacteria are thought to play an important role in inducing protective immunity and to develop new methods for diagnosing tuberculosis. Methods: A culture filtrate protein of M. avium that was strongly reactive with goat antiserum against M. intracellulare was constructed. Its homologous protein (TB-14) in M. tuberculosis was cloned, expressed and purified. The inductions of IFN-${\gamma}$ stimulated with $10{\mu}g$ of TB-14 recombinant protein and $10{\mu}g$ PPD were estimated by using whole bloods from seven PPD (-) subjects, seven PPD (+) healthy volunteers and nine tuberculosis patients. Results: M. avium culture filtrate protein was confirmed as a hypothetical protein that was termed contig 116. A novel 14-kDa recombinant protein (TB-14) of M. tuberculosis was composed of 148 amino acids, including 30 amino acids of the signal peptide, and it showed 78% homology with M. avium. In the PPD (+) healthy volunteers, recombinant TB-14 protein strongly induced the secretion of IFN-${\gamma}$ in whole blood cultures. Conclusion: These results suggest that TB-14 recombinant protein might play an important role in inducing cell-mediated immunity against tuberculosis. Furthermore, TB-14 protein antigen and its antiserum will be available for the development of new diagnostic tools for tuberculosis.

• Journal of the Korea Convergence Society
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• v.9 no.10
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• pp.315-324
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• 2018

The study examines the effects of knowledge combination and of its interaction with technological change on the usefulness of inventions. We argue that inventing with knowledge components of prior art or with those in a variety of technical fields results in useful inventions, which changes after the emergence of dominant design because external actors' perception of which knowledge components are appropriate in current technological environments changes. Based on data from U.S. granted optical disc patents filed from 1992 to 2000, the results show that inventions with more new knowledge components relative to their prior art are less useful but that inventions with more diversified knowledge components are more useful. Also, the empirical findings show that the negative relationship between new knowledge components of inventions and their usefulness strengthens after dominant design emerges.