• Journal of Life Science
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• v.15 no.3 s.70
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• pp.486-491
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• 2005

Physical activity can improve sensorimotor recovery after peripheral nerve injury. Growth-associated protein 43 (GAP-43) is highly correlated with neuronal development and axonal regeneration and present in large quantities in the axonal growth cone. Using immunofluorescene staining and anterograde and retorgrade techniques, we identified enhanced axonal regrowth in distal stump of the sciatic nerve 3-14 days after crush injury in rats with treadmill training. We also carried out western blot to investigate GAP-43 protein expression in injured sciatic nerve. GAP-43 protein levels were highly induced in the injured sciatic nerve 3, 7 and 14 days compared with sedentary group. Thus, the present data provide a new evidence that treadmill training promoted axonal re-growth after injury and increased GAP-43 protein levels in the regenerating nerve.

• Microbiology and Biotechnology Letters
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• v.16 no.2
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• pp.150-155
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• 1988

In order to produce L-lysine from cellulosic substrates by the intergeneric protoplast fusion between cellulolytic bacteria, Cellulomonas flavigena KFCC31221 and amino acid producing bacteria, Brevibacterium flavum ATCC14067, Corynebacteriurn glutamicum ATCC13032, conditions for protoplast formation and regeneration of these strains were investigated. After the strains were mutated with 500$\mu\textrm{g}$/$m\ell$ N-methyl-N'-nitro N-nitrosoguanidine for 30 min and the mutants were enriched by treating 300$\mu\textrm{g}$/$m\ell$ penicillin-G for 2 hrs, B. flavum Hse- Str$^{r}$ , C. glutamicum Met$^{-}$Thr$^{-}$ Rif$^{r}$ and Cellulomonas flavigena Thr$^{-}$Val$^{-}$Kan$^{r}$ were isolated. The rate of protoplast formation ranged from 95 to 98% when strains were treated at the concentration of 500$\mu\textrm{g}$/$m\ell$ of lysozyme, pH 6.5, 33$^{\circ}C$, for 6 hrs. in Tris- malate buffer supplemented with 0.4M sucrose as osmotic stabilizer. Approximately 30-33% protoplast was regenerated on the regeneration complete medium(RCM) containing 1.5% agar and 0.5M sodium succinate overlaid with the same medium except 0.7% agar.

• Polymer(Korea)
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• v.32 no.6
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• pp.516-522
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• 2008

Biodegradable polymers have been used extensively as scaffolding materials to regenerate new tissues and the ingrowth of tissue have been reported to be dependent directly of the porosity, pore diameter, pore shape, and porous structure of the scaffold. In this study, porous poly (L-lactide-co-glycolide) (PLGA) scaffolds with five different pore sizes were fabricated to investigate the effect of pore sizes for AF tissue regeneration. Cellular viability and proliferation were assayed by MTT test. Hydroxyproline/DNA content of AF cells on each scaffold was measured. sGAG analyses were performed at each time point of 2 and 6 weeks. Scaffold seeded AF cells were implanted into the back of athymic nude mouse to observe the difference of formation of disc-like tissue depending on pore size in vivo. We confirmed that scaffold with $180{\sim}250{\mu}m$ pores displayed high cell viability in vitro and produced higher ECM than scaffold with other pore sizes in vivo.

• 한국신재생에너지학회:학술대회논문집
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• 2010.06a
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• pp.223.1-223.1
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• 2010

CFRP는 우수한 기계적 강도와 경량 특성으로 인하여 NGV/FCV용 가스를 저장하기 위한 재료로서 널리 이용되고 있다. 탄소 섬유와 에폭시로 이루어진 CFRP는 화염 노출 시 매트릭스의 열적 분해 반응에 의해 급격한 물성 변화를 일으킨다. CFRP 메트릭스가 100kW/$m^2$ 이하의 열플럭스에 노출되는 경우 표면온도 변화에 따른 용기 내부로의 열확산 메카니즘을 규명하기 위해서는 시간에 따른 경계조건의 변화를 명확히 할 필요가 있다. 본 연구에서는 Fuel bed type 가열장치의 열플럭스를 계산하였으며 계산된 열플럭스에 노출되는 CFRP 용기 표면의 온도 변화 측정 실험을 수행하였다. 또한 측정 결과를 보고된 문헌의 결과와 비교하였다.

• 한국신재생에너지학회:학술대회논문집
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• 2009.06a
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• pp.434-435
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• 2009

최근 바이오매스를 친환경 소재로 활용하기 위한 연구개발 및 응용연구가 세계적으로 활발하게 진행되고 있다. 바이오매스를 기반으로 한 대표적인 친환경 소재는 바이오복합재료로서 현재 자동차의 내 외장재료로 사용되고 있으며 응용분야가 전자재료, 포장재료 등으로 다양하게 확대되고 있다. 바이오매스는 이산화탄소 흡수원일 뿐만 아니라 바이오매스를 이용한 바이오복합재료는 경량재료로서 자동차에 사용될 때 연비향상에 의해 이산화탄소 저감에 크게 기여할 수 있다. 최근에는 바이오매스를 기반으로 하여 고강도 특성 등 기존재료에 비해 우수한 특성을 가지는 나노소재를 제조하고 이를 다양한 에너지소재로 활용하기 위한 연구도 활발하게 진행되고 있다. 본 논문에서는 바이오매스를 기반으로 한 친환경 소재로서 바이오매스를 기반으로 한 바이오복합재료 및 친환경 나노소재의 연구동향 및 응용분야에 대해 소개하고자 한다.

• New & Renewable Energy
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• v.4 no.4
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• pp.50-55
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• 2008

Recently, sea algae cultivation as carbon sink and carbon dioxide fixation have been considered. Also, various researches on bioenergy derived from sea algae and the utilization of fibers, saccharide, and lipid of sea algae have been performing. Till now, algae fibers has been used for manufacturing of paper and reinforcing of polymer composites and the extracts of sea algae are used for cosmetics, pharmaceutical materials and food such as agar. Especially, algae fiber has so similar properties to cellulose in terms of crystallinity and functional groups that it can be utilized as reinforcements of biocomposites. Biocomposites as alternatives of glass fiber reinforced polymer composites are environmentally friendly polymer composites reinforced with natural fibers and are actively applying to the automobiles and construction industries. In this paper, characteristics of algae fiber and biocomposites reinforced with algae fiber as environmentally friendly energy materials have been introduced.

• Journal of the Korean Arthroscopy Society
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• v.12 no.3
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• pp.147-153
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• 2008

Several methods to resurface the lost cartilage of the knee have been used; multiple drilling, microfracture, abrasion arthroplasty. Resurfaced cartilages resulting from above techniques are mostly fibrocartilage. Autologous osteochondral graft transfer and autologous chondrocyte transfer are known to resurface the lost cartilage with mostly hyaline cartilage. This article reviews basic researches, indications, operative technique, and clinical results of autologous osteochondral graft transfer.

• 한국신재생에너지학회:학술대회논문집
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• 2007.11a
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• pp.331-334
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• 2007

Wind turbine is composed by 3 major parts, rotor ass'y, nacelle ass'y and tower. There are two major point in nacelle cover analysis one is nacelle cover itself the other is cover support structure. Both of them are required strength proof with light weight. For the design of structure, the loads are calculated according to GL wind guideline Ed. 2003 and by the commercial F.E. codes,

• New & Renewable Energy
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• v.4 no.3
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• pp.45-50
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• 2008

GFRP based composite rotor blades were developed for 750 kW & 2 MW wind turbines. The blade sectional geometry was designed to have a general shell-spar and shear web structure. For verifying the structural safety under all relevant extreme loads specified in the GL guidelines, the structural analysis of the rotor blades was performed using commercial FEM codes. The static load carrying capacity, blade tip deflections and natural frequencies were evaluated to satisfy the strength and stability requirements. Full-scale proof tests of rotor blades were carried out with optical fiber sensors for real-time condition monitoring. Finally, the prototype of each rotor blade passed all proof tests for GL certification.

• 한국신재생에너지학회:학술대회논문집
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• 2008.10a
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• pp.328-331
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• 2008

For the purpose of verifying the calculation, the rotor blade shall be subjected to test for the natural frequencies and the static loading within the scope of the assessment. This paper presents a full scale static test procedure of the rotor blade for certification by GL. This blade model is manes as KM24 designed for IEC type IA. The test and calculation values are all most similar. Also there is not founded any marks of cracks or buckling at the shell, and bonding area is T/E, L/E and shear web. Therefore, the test is successful and the rotor blade is satisfied the safety requirement at the maximum design load.