• Journal of Mushroom
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• v.5 no.2
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• pp.51-58
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• 2007

These experiment was conducted to find the superior strain selection, cultivation technique and optimum environmental condition of nameko mushroom culture using plastic container. The results was following as Mycelium of Pholiota nameko grown well at MCM and Hamada media, and its media acidity was pH 6~7. The optimum temperature condition for growing mycelium was $25^{\circ}C$. Under $15^{\circ}C$ and above $30^{\circ}C$ of temperature condition, mycelium growing speed was delayed remarkably. Among the 29 strains of nameko mushroom, the most productive strains was JNM19007, JNM19026, JNM19027 and JNM19028. The optimum media composition rate for produce fruitbody was pine sawdust 80% + wheat bran 20%. In this condition, the average fruitbody amount was 188g per 1,100cc container. The optimum post-culturing period was 50 days and mushroom sprout appeared 7 days after old mycelia removed. The suitable temperature was $12^{\circ}C$ for induce sprout, growing period was $16^{\circ}C$ and the optimum relative humidity was 95% in all culturing periods.

• Korean Journal of Organic Agriculture
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• v.19 no.1
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• pp.93-107
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• 2011

This research was carried out to investigate invertebrate fauna with organic and conventional pear orchards, which used four collected methods; soil sampling for soil microorganism, pitfall, malaise, and black light trap for over ground species. Collected species were 37 species, 1,184 individuals in organic and 28 species, 501 individuals by soil sampling in conventional pear fields. Those were 38 species, 646 individuals and 29 species, 440 individuals by pitfall trap, 55 species 650 individuals and 47 species, 508 individuals by malaise trap, and 23 species, 201 individuals and 9 species, 42 individuals by black light trap. Collembola was collected 389 individuals in organic which was 5 times than in conventional in soil sampling. In pitfall trap, that was 183 individuals which was 3 times. The diversity indices of organic pear orchards were 1.956 in May, 2.638 in August and those of conventional was 1.426 in May, 2.011 in August in soil sampling. In pitfall trap, the dominant species were spiders, collembollan, and coleopteran. Among Coleoptera, indicator insects for the evaluation of agricultural environment suggested were Eusilpha jakowelewi as organic pear orchard and Anisodactykus punctatipennis and Pheropsophus jessoensis as conventional. Malaise trap was collected dominant species as Diptera and Hymenoptera of Braconidae and Ichneumonidae. The diversity indices of organic pear orchards were 2.952, 3.120, and 2.010 in pitfall, malaise and black light trap in over ground invertebrate sampling. The highest diversity was in malaise trap. The higher diversity indices, the lower dominance indices.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.21-21
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• 2018

본 연구는 북방계 식물에 속하는 두메우드풀[Woodsia ilvensis (L.) R.Br.]의 전엽체 증식 및 포자체 형성을 위한 적정방법을 구명하고자 수행되었다. 식물재료는 경상북도 의성군 일대에서 채취한 후 청주의 일반하우스에 이식하여 재배하였다. 포자 성숙기인 8월에 채종하여 발아시켰으며, 포자로부터 획득한 전엽체를 8주간 계대하면서 실험재료로 사용하였다. 전엽체 300mg을 메스로 다진 후 배지농도 및 종류(Knop, 1/4, 1/2, 1 및 2MS)를 달리하여 8주간 배양하였다. 배양실의 온도는 $25{\pm}1.0^{\circ}C$, 광도는 $30{\pm}1.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h)로 조절되었다. 연구의 결과, 배지구성물질의 농도가 높았던 2MS배지에서 전엽체의 생체중이 12.4g으로 41배 증가하여 가장 높은 증식율을 나타냈다. 또한 배지구성물질의 농도가 적을수록 생체중의 증가폭도 함께 감소하는 경향을 보였다. 포자체 형성에 적합한 토양조건을 확인하고자, 원예상토, 피트모스, 펄라이트 및 마사토의 비율을 달리한 5종류의 혼합토양을 조성하여 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하였다. 다음으로 전엽체 1g을 핸드블랜더로 10초간 분쇄 후 충진된 사각분 위에 고르게 분주하여 14주간 재배하였다. 재배환경은 온도 $25{\pm}1.0^{\circ}C$, 광도 $43{\pm}2.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h) 및 습도 $72{\pm}2.0%$로 조절되었다. 지베렐린 수용액이 포자체 형성에 미치는 영향을 조사하고자, 선행연구에서 선발된 토양조건을 기준으로 침지농도(0, 50, 100, $200mg{\cdot}L^{-1}$)를 조절하여 1시간 침지 후 상기와 동일한 방법으로 재배하였다. 연구의 결과, 원예상토와 펄라이트 또는 마사토를 2:1(v:v)로 혼용한 토양에서 사각분 당 109.5, 128.8개의 포자체가 형성되었다. 한편, 피트모스가 첨가된 처리는 포자체의 형성이 억제되는 경향을 나타냈다. 지베렐린 50과 $100mg{\cdot}L^{-1}$ 수용액에 침지한 처리에서 무처리에 비해 많은 사각분 당 각 177.0, 181.7개의 포자체가 형성되었으며, 생육도 양호하였다.

• Journal of Bio-Environment Control
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• v.9 no.2
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• pp.85-93
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• 2000

This study was carried out to investigate the effects of several substrates and ratio of NO3--N to NH4--N in nutrient solution on growth, yield and mineral uptake of sweet pepper(Capsicum annuum L.) in bag culture. The content of minerals such as P, K, Ca and Mg showed difference in concentration among media: P and Mg were the highest in vermiculite in vermiculti+rice hull, K in perlite+vermiculite and Ca in perlite+peatmoss; with the lowest in the single perlite medium respectively, Ca of mineral made fairly higest level in concentration compared with the others in all of the media. The concentration of mineral content was lower in the ratio of 8:2 than that of 10:0. Admitting that the pH made difference depending on the kind of substrates and ratios of NO3--N to NH4--N. The pH of 10:0 ratio in all the substrates was higher and more stable than that of 8:2. The range of EC in all the substrates showed from 1.78ds·m-1 to 2.10 ds·m-1, which was optimum range for growth of sweet pepper, and range of EC is larger in 8:2 ratio than that in 10:0 ratio. Plant height and stem diameter were nothing to do with the kind of substrates, but leaf area was the largest at vermiculite+rice hull of the 8:2 ratio, fresh and dry weights were heavier at peatmoss+carbonized rice hull, but were the lightest at perlite. All indexes related to the growth which had something to do with the kind of substrates higher in 8:2 ratio than those in 10:0 ratio. The number of fruit and fresh weight related to the ratio of the 8:2 were the highest as 17.5 at vermiculite+rice hull with 1,588g of fresh weight, while the yield from perlite was the lowest. The number of fruit was the highest as 16.4 at virmiculite+rice hull, yield was the higest as 1,394a at perlite+ peatmoss. The yield of 8:2 ratio at all substrates was higher than that of 10:0 ratio. Of the mineral content related to the plant part, K+ and Mg2+ were higher in concentration at leaf; Ca2+ were higher at root; PO4- was higher at stem and fruit; The content of mineral showed no difference between the ratio of the 8:2 and the ratio of the 10:0 with no regrading to the difference of mineral content among substrates; and K+, Ca2+, and Mg2+ uptake of sweet pepper were higher at 10:0 ratio than that of 8:2; 2while PO4- uptake of sweet pepper was lower at 10:0 ratio than that of 8:2 ratio.

• Journal of Mushroom
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• v.13 no.4
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• pp.301-304
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• 2015

This study was carried out to investigate the optimum rate of substrate composition in bottle cultivation of Grifola frondosa and had three rates of substrate composition of 67:11:22(T1), 68:15:17(T2) and 74:14:12(T3) as mixing rate of weight of dried oak sawdust, dried corn husk and dried bean-curd refuse. The rate of primordia formation of T3 was 65.8% which was lowest among all treatments. Contraction rate of disease of T1 was 9.8% which was highest among all treatments. Harvesting rate of T2 was 70.5% which was highest among all treatments. Fruit body weights per bottle of T1 and T2 were 85.5 g, 83.3 g respectively and there was not significant difference between those. Yield per 10,000 bottles of T2 was 587 kg and was 7%, 28% higher than those of T1 and T3, respectively. As a result, the rate of substrate composition of 68:15:17(T2) as mixing rate of weight of dried oak sawdust, dried corn husk and dried bean-curd refuse was appeared as optimum rate of substrate composition in bottle cultivation of Grifola frondosa..

• Korean Journal of Medicinal Crop Science
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• v.15 no.4
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• pp.261-265
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• 2007

A climatic feature, growth condition and incidence phase of disease and pest in Korean ginseng (Panax ginseng C. A. Meyer) were surveyed, both the conventional cultivation and culture in the forest located in Jinan. The results were as follows. The forest field was lower $1^{\circ}C$ in the air temperature, lower $1.9^{\circ}C$ in the soil temperature and 11.8% lower in the soil temperature compared with the conventional field. The wind velocity of the forest field was three times slower, but the dew condensation time was 3.3 hours longer than the conventional field. Particularly, the amounts of solar radiation in the forest was remarkably lower, while the rainfall was little lower with no significance. The incidence of disease and pest in the forest field was severe compared with that of the conventional field. Among them, Colletotrichum gloeosporioides, Cylindrocarpon destructans, and Alternaria panax was severe in the forest field while, Botrytis cinerea was severe in the conventional field. The growth condition of forest field in Panax ginseng C. A. Meyer was 50% worse in general and the root weight was 87.2% less than the 3-year-old ginseng in the conventional field.

• The Korean Journal of Mycology
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• v.30 no.2
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• pp.99-103
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• 2002

The suitable sterilization conditions in pot cultivation of varieties of Chunchunutari-2-ho and Suhannutari-1-ho Pleurotus ostreatus were investigated. The substrate formulations for the cultivation consisted of 40% poplar sawdust, 40% waste cotton and 20% beet pulp (PWB), and 40% poplar sawdust, 40% cotton seed hull and 20% beet pulp (PCB). The increase in the sterilization temperature caused decrease in the pH of the substrate. The moisture content of PWB substrate increased by 1.8%, but that of PCB substrate decreased by 4.1 % after low temperature sterilization ($12hr\;at\;60^{\circ}C$). However, a little change in the sterilization at higher temperature ($6hr\;at\;100^{\circ}C\;and\;90min\;at\;121^{\circ}C$) did not alter the moisture content of both substrate appreciably. The duration of mycelial growth was longest and its density was lowest in low temperature sterilization ($12hr\;at\;60^{\circ}C$). The high pressure sterilization ($90min\;at\;121^{\circ}C$) of PCB substrate produced high yield (236.6 g/pot) and the highest biological efficiency (96.6%) for Chunchunutari-2-ho. On the contrary the high temperatures sterilization ($6hr\;at\;100^{\circ}C$) of PCB substrate resulted in the yield of 259.1 g/pot with the biological efficiency 94.9% for Suhannutari-2-ho.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.150-154
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• 2016

To determine a favorable substrate formulation for Grifola frondosa, physicochemical conditions, culture properties, and yields according to various substrate formulations were investigated. Based on these analyses, T4 (80:5:15 ratio of oak sawdust to dried bean-curd refuse to corn husk) resulted in a shorter cultivation period and higher yields (weight of fresh mushrooms harvested at maturity) than those of other treatments. The physicochemical properties of T4 were pH 5.4, 2.4% crude fat contents, 54 C/N ratio, 74.3% porosity, and 0.26 g/cm3 bulk density. These results emphasize the importance of optimal substrate development on the production efficiency of G. frondosa mushrooms and have implications for commercial applications.

• The Korean Journal of Mycology
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• v.40 no.1
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• pp.49-53
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• 2012

Hot-water extracted natural media were made from raw materials for mycelial culture of Pleurotus eryngii. Poplar sawdust, wheat bran and rice bran were used as substrates for hot water extraction. The mixed substrates of poplar sawdust, wheat bran, and rice bran with 50 : 20 : 30 (v/v/v, PWR523) and 50 : 30 : 20 (v/v/v, PWR532) were optimal for mycelial growth of P. eryngii, respectively. The hot-water extracted natural media from PWR523 and PWR532 showed a rapid mycelial growth and spawn running compared to PDA. There was no significant difference in mushroom yield when the mycelium grown on the hot-water extracted natural media was used as the inoculum source for producing fruit body.

• Journal of Mushroom
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• v.10 no.4
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• pp.236-243
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• 2012

Spent mushroom substrates is composted organic material remaining after a crop of mushroom is harvested. The raw materials of mushroom substrates are same feed ingredient as corncobs, rice brown, wheat brown, cotton seeds and beet pulp. During the mushroom cultivation process, the mushroom substrates was used 15-25% by mushroom and 75-85% of mushroom substrates was remained in the SMS. Among of the spent mushroom substrates, spent mushroom substrates of pleurotus eryngii, Pleurotus ostreatus and Flammuliua velutipes is can be use the energy feedstock of animal feed. The cellulose content of spent mushroom(pleurotus eryngii) substrates containing the sawdust was high and total digestible nutrients (TDN) values was low. The spent mushroom(pleurotus eryngii) substrates fermented with cellulase and xylanase producing bacteria is may be used as an ingredient of feed in TMR for Hanwoo steer.