• Title/Summary/Keyword: 재래닭

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The +1316 T/T Genotype in the Exon 3 of Uncoupling Protein Gene is Associated with Daily Percent Lay in Korean Native Chicken (한국 재래 닭의 Uncoupling Protein 유전자 Exon 3에서의 +1316 T/T 유전자형이 산란율에 미치는 효과 분석)

  • Oh J. D.;Lee J. H.;Hong Y. S.;Lee S. J.;Lee S. G.;Kong H. S.;Sang B. D.;Choi C. H.;Cho B. W.;Jeon G. J.;Lee H. K.
    • Korean Journal of Poultry Science
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    • v.32 no.4
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    • pp.239-244
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    • 2005
  • Uncoupling protein(UCP) is expressed exclusively in brown adipose tissue(BAT). It is blown to uncouple phosphorylation from oxidation and hence to be involved in energy metabolism and heat production, especially under cold exposure. In the present study, we identified single nucleotide polymorphism(SNP) in exon 3 of avUCP gene in Korean native chicken(KNC) population. It was detected a SNP T+1316C in exon 3 of avUCP gene by sequence analysis in KNC population. For PCR-RFLP analysis of the SNP T+1316C, used by AP III restriction enzyme. The result of PCR-RFLP analysis showed that allele T has two fragments of 255 bp and 86 bp, and allele C has only one fragment of 341 bp. The genotype frequencies were TT type, 0.7875; TC type, 0.1875 and CC type, 0.025; and the frequencies of allele T and C were 0.881 and 0.119, respectively in KNC population. Next study was conducted to investigate the effect of the SNP in avUCP gene on economic traits in the KNC population. The TT genotype had a significant higher daily percent lay(84.61) than CC genotype(p<0.05) in KNC population. This study may be useful for genetic studies of avCUP gene and selection on daily percent lay of KNC.

A Study on the Production Performance of Korean Native Chickens for Samgye Chicken Production (삼계용 토종닭의 생산능력 고찰)

  • Kigon Kim;Hyun-Wook Kim;Hyo Jun Choo;Jung Min Heo;Ki Suk Oh;Sang-Hyon Oh;See Hwan Sohn
    • Korean Journal of Poultry Science
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    • v.50 no.2
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    • pp.91-99
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    • 2023
  • This study aimed to develop a high-productivity breed of Korean native Samgye chicken. We evaluated the production performance of six Korean native chicken combinations (KNC-SCYC, SCYD, SDYC, SDYD, SYYC, SYYD) and GSP-Hanhyup Korean native chickens, with Baeksemi chickens used as a control group. The performance test was conducted from hatching to 7 weeks of age on 756 chickens, and we measured survival rate, body weight, shank length, feed utility, and carcass yield. The overall survival rate was nearly 100% for all strains. However, body weight showed significant differences between strains at all ages (P<0.01), with Baeksemi weighing 863.8±76.9 g, GSP-Hanhyup weighing 804.7±72.5 g, and KNC-combinations weighing 543.0±61.8 g at 5 weeks of age. The duration needed to reach 850 g was estimated to be 34.5 days for Baeksemi, 37.5 days for GSP-Hanhyup, and 45.8-48.8 days for KNC-combinations. Carcass yield percentage was highest for KNC-SYYD combination at 63.3%, followed by Baeksemi at 60.4%, and GSP-Hanhyup at 56.1%. Shank length at 850 g body weight was 7.6 cm for KNC-SYYD combination, 7.8 cm for Baeksemi, and 8.0 cm for GSP-Hanhyup. The feed conversion ratio at 850 g body weight was 1.81 for Baeksemi, 2.17 for GSP-Hanhyup, and 2.27 for KNC-SCYC combination. Our results suggest that the KNC-SYYD combination and GSP-Hanhyup breed have the potential to be used in Samgye production due to their moderate growth performance, higher carcass yield, and shorter shank length, despite their lower growth productivity and feed efficiency when compared to Baeksemi.

Genetic Polymorphism of Avian Leukosis Virus Host Receptors in Korean Native Chickens and Establishment of Resistant Line

  • Lee, Kyung Youn;Shin, Yun Ji;Han, Jae Young
    • Korean Journal of Poultry Science
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    • v.49 no.2
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    • pp.99-108
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    • 2022
  • Avian leukosis virus (ALV) is a highly contagious retrovirus that causes tumors and has resulted in great economic loss worldwide owing to its high transmission rate. Various ALV viral subgroups exist, with infections occurring via specific host receptors. The susceptibility or resistance of avian species to the ALV-A and K subgroups is determined by the host receptor, the tumor virus locus A (tva) gene, while that to ALV-B depends on another host receptor, the tumor virus locus B (tvb) gene. The resistance alleles of tva and tvb have primarily been identified in China, but none have beendetected in Korea. We analyzed the frequencies of tva and tvb genotypes in White Leghorn (WL), Korean Ogye (KO), and Korean native chicken (KNC) breeds, and assessed the resistance to ALV subgroups. In WL, both tva and tvb had various genotypes, including susceptibility and resistance alleles, whereas in KO, tva and tvb resistance alleles were dominant. In KNC, tva susceptibility and resistance alleles were mixed, whereas tvb resistance alleles were dominant. In addition, we showed that there were differences in the splicing pattern of tva transcripts and the expression level of tvb transcripts within breeds. Finally, we confirmed that ALV resistance depended on KO and KNC genotypes by in vitro infection of chicken embryonic fibroblasts with ALV. These results highlight that some KO and KNC individuals are naturally resistant to ALV subgroups A, B, and K, and will facilitate the preservation of economically superior traits through selective breeding.

산란형질에 대한 한국 재래닭 계통간 교배종의 잡종강세 및 결합능력 추정

  • 최철환;상병돈;김학규;조규호;정일정;상병찬;박창식;한성욱
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2002.11a
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    • pp.140-142
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    • 2002
  • This study was conducted to obtain some genetic information for developing superior Korean native chickens. Heterosis and combining ability effects were estimated with 1,080 progenies of full diallel crosses of 3 strains in Korean Native Chicken. The traits of egg productivity were Investigated, and heterosis effect, general combining ability, specific combining ability and reciprocal effect were analyzed model I of the Griffing's method 1. The general performance were 147.0 days in age at first egg laying, 71.8 eggs in egg numbers to 270 days, 50.1g in egg weight at 270 days, 75.6 in egg shape index, 356$\mu\textrm{m}$ in egg shell thickness, and 4.27kg/$\textrm{cm}^2$ in egg shell strength, respectively. According to result, heterosis effect revealed comparatively high in age at first egg laying, egg numbers, egg weight, and egg shell strength. The effects estimated highly in general combining ability for age at first egg laying, egg numbers, egg weight, egg shape Index and egg shell thickness, and in specific combining ability were egg numbers, egg weight and egg shell thickness, and in reciprocal effects for egg numbers, egg shape index, and egg shell thickness. This result could be used for developing Korean native chicken.

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Comparison of Stress Response between Korean Native Chickens and Single Comb White Leghorns subjected to a High Stocking Density (닭의 품종 간 스트레스 반응 정도 비교 분석)

  • Sohn, Sea Hwan;Cho, Eun Jung;Park, Dhan Bee;Jang, In Surk;Moon, Yang Soo
    • Korean Journal of Poultry Science
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    • v.41 no.2
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    • pp.115-125
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    • 2014
  • With Single Comb White Leghorn (WL) and Korean Native Chicken (KNC) breeds, we compared the stress response with chicken breeds that were subjected to a high stocking density. Stress response was analyzed by the quantity of telomeric DNA, the rate of DNA damage and the expression levels of heat shock proteins (HSPs) and hydroxyl-3-methyl-glutaryl coenzyme A reductase (HMGCR) genes on tissues and blood. The telomere length and telomere shortening rates were analyzed by quantitative fluorescence in situ hybridization on the nuclei of lymphocytes and tissues. The DNA damage rate of lymphocytes was quantified by the comet assay. The expression levels of HSP70, HSP90-${\alpha}$, HSP90-${\beta}$ and HMGCR genes were measured by quantitative real-time polymerase chain reaction in lymphocytes. There was no significant difference between KNC and WL in body weight, weight gain, telomere shortening rate and DNA damage rate. However, the growth rate significantly decreased in chickens raised under high stocking density conditions, as compared to the control group. The telomere-shortening rate, DNA damage and HSPs expression of the lymphocytes were significantly higher in the high stocking density group than the control. The stress condition and breeds had a significant effect on the expressions of HSP70, HSP90-${\alpha}$ and HSP90-${\beta}$ in lymphocytes, except HMGCR. The stress response of WL was higher than that of KNC, as analyzed to the expression of HSP70 and HSP90-${\alpha}$. Therefore, we concluded that the chickens which were exposed to a high stocking density had increased the individual physiological stress response regardless of breeds, and White Leghorns are more susceptible to stress condition than Korean Native Chickens.

Effects of Dietary Energy, Protein on Growth and Blood Composition in Cross-bred with Korean Native Chicks (사료 단백질 및 에너지 수준이 재래닭의 성장과 혈액의 성상에 미치는 영향)

  • Lee, H.S.;Kang, B.S.;Na, J.C.;Ryu, K.S.
    • Korean Journal of Poultry Science
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    • v.35 no.4
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    • pp.399-405
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    • 2009
  • Two experiments were conducted to establish the basic data of feeding system in cross-bred Korean native chicks. A total of seven hundred twenty chicks were replaced the 36 floor pen for the first five weeks with $3{\times}3$ factorial design in Experiment 1. Four hundred eighty of five weeks old chicks were raised from six to ten weeks of age with $3{\times}2$ factorial design in Experiment 2. Dietary ME and CP were formulated to contain 3,000, 3,050, 3,100 kcal/kg and 21, 22, 23%, respectively in Experiment 1 and 3,050, 3,100, 3,150 kcal/kg and 18, 19% in Experiment 2. Weight gain, feed intake were measured and calculated the feed conversion. Blood were collected and analyzed at the end of experiments. In Experiment 1, weight gain showed significantly higher in 3,050, 3,100 kcal/kg treatments than 3,000 kcal/kg treatment (P<0.05), but was not different in CP treatments. Feed intake was statistically high in 3,000 kcal/kg treatment compared with 3,050 and 3,100 kcal/kg ones (P<0.05), and more increased in 21% CP treatments compared to that of 22 and 23 CP treatment (P<0.05). Feed conversion of birds fed 3,050 and 3,100 kcal/kg diet showed much lower than 3,000 kcal/kg treatments (P<0.05). FCR was signicantly improved (P<0.05) in chicks fed diets containing 21 and 22% CP as compared to that fed 20% CP. Blood protein, glucose, and total cholesterol tended to increase in high energy and diet treatments. Blood HDL was increased as dietary energy increased, whereas LDL increased in low CP treatments. In Experiment 2, weight gain was not consistent between treatments, but more increased in 18% CP treatments compared to that of 19% CP treatment from six to ten weeks old in cross bred chicks (P<0.05). Feed intake was similar to the result of weight gain, but more increased in 19% CP treatment than 18% CP treatment (P<0.05). There were no statistically difference in FCR, but seemed to improve as dietary ME increased. Blood total protein and glucose increased as dietary CP was high, but triglyceride and HDL increased in high versus low ME (P<0.05). The results of these experiments suggested that optimum dietary ME and CP, were 3,050, 3,150kcal/kg and 22, 19% for the first five weeks and second one, respectively.

Comparison of Physicochemical Characteristics of the Meat in Four Lines of Korean Native Chickens (4계통 재래종 닭고기의 이화학적 특성 비교)

  • Lee, Seung Gyu;Utama, Dicky Tri;Baek, Ki Ho;Park, Young Hyun;Han, Jae Yong;Lee, Sung Ki
    • Korean Journal of Poultry Science
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    • v.42 no.4
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    • pp.335-345
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    • 2015
  • This study was conducted to compare carcass yield and meat quality among four lines of Korean native chickens (Yeonsan Ogye, Hyunin Black, Hoengseong Yakdak and Hwangbong) and White Leghorn as control. Chickens (N=23, male) were grown under same conditions and slaughtered at 56 weeks old to observe the physicochemical differences in breast and leg meats. The live and carcass weights of Hwangbong were significantly higher than other chickens (p<0.05). Four lines of Korean native chickens, regardless of the part, had higher shear force value than White Leghorn (p<0.05). In addition, the breast meat of Korean native chickens had higher cooking loss and lower water holding capacity than that of White Leghorn. The cooked meat of Hwangbong particularly were more chewy, gummy and cohesive with lower tenderness than other chickens (p<0.05). Oleic acid content which is related to meat flavor was significantly higher in the breast meat of Hoengseong Yakdak (39.6%). The meat of Korean native chickens had lower n6/n3 ratio, in which the breast and leg meat of Hoengseong Yakdak contained the lowest ratio (p<0.05). The meat of Hwangbong and Hoengseong Yakdak were more acceptable than the others according to sensory test (p<0.05).

Genetic Variations of Chicken TYR Gene and Associations with Feather Color of Korean Native Chicken (KNC) (한국 토종닭 모색 변이와 TYR 유전자형 간의 상관관계 분석)

  • Choi, Jin Ae;Lee, Jun-Heon;Jang, Hyun-Jun;Lee, Kyung-Tai;Kim, Tae-Hun;Lee, Hyun-Jeong;Heo, Kang-Nyeong;Kim, Chong-Dae;Han, Jae-Yong;Park, Mi Na
    • Korean Journal of Poultry Science
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    • v.41 no.1
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    • pp.7-14
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    • 2014
  • Tyrosinase (TYR) gene is located on chromosome 1 in chicken and it is composed of five exons and four introns. TYR gene is described as a key enzyme in melanin biosynthesis. Most examples of complete albinism in chicken have been due to defects in the tyrosinase gene. The association of feather color and sequence polymorphism in the Tyrosinase (TYR) gene was investigated using Korean Native chicken H breed (H_PL), Korean Native chicken L/W breed(L/W_PL) and 'Woorimatdag' commercial chickens (Woorimatdag_CC). From L_PL and W_PL breed analyses, 4 synonymous SNPs (locus G33A, G116A, C217T and C247T) and 2 SNPs (G838A and G958A) were detected in 4th exon and 4th intron of TYR gene respectively. The genotype frequencies for 6 SNPs were compared between L_PL and W_PL and W_PL represented homozygous SNP types in all the analyzed SNP positions while L_PL displayed various SNP types.