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Antioxidant Activities of Stachys sieboldii MIQ Roots (초석잠(Stachys sieboldii MIQ) 뿌리 추출물의 항산화 활성 고찰)

  • 백홍석;나영수;김도한;이창한;류병호;송승구
    • Journal of Life Science
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    • v.14 no.1
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    • pp.1-7
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    • 2004
  • The root of Stnchys Sieboldif MIQ was extracted three times with methanol and extract was found to contain 3.02% of polyphenols and 1.97% of flavonoids. DPPH radical scavenging method, ferric thiocyanate method, and nitrite scavenging ability method were employed to investigate the constituents of the extract and to measure their activity on antioxidation. The fraction extracted by ethylacetate showed higher anti oxidation value than that of $\alpha-tocopherol$, butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) at the same concentration. UV-VIS spectral data of the extract by ethylacetate that was isolated on a silica gel column proved adsorption maxima in the range of 280∼330 nm. The fraction ES-RS that has $\lambda_{max}(nm)$ of band 1, 325nm and band II, 289nm exhibitd the strongest activity on antioxidation. ES-R5 fraction showed similar pattern to flavones by the analysis of UV-VIS spectral data.

Optimal Conditions for As(III) Removal by Filtration System Packed with Different Ratio of Iron-Coated Sand and Manganese-Coated Sand (철 및 망간코팅사 충전비를 달리한 여과시스템에서 3가 비소 제거의 최적 조건)

  • Chang, Yoon-Young;Kim, Kwang-Seob;Song, Ki-Hoon;Yang, Jae-Kyu
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.11
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    • pp.1186-1191
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    • 2006
  • Removal efficiency of As(III) through oxidation and adsorption in column reactors was investigated at different ratios of manganese-coated sand(MCS) and iron-coated sand(ICS) : MCS-alone, ICS-alone and both of ICS and MCS. The breakthrough of arsenic immediately occurred from a column reactor with MCS-alone. However, most of the arsenic present in the effluent was identified as As(V) due to the oxidation of As(III) by MCS. While five-times delayed breakthrough of arsenic was observed from a column reactor with ICS-alone. At a complete breakthrough of arsenic, the removed As(III) was 36.1 mg with 1 kg ICS. To find an optimum ratio of ICS and MCS in the column packed with both ICS and MCS, the removal efficiency of As(III) was investigated at three different ratios of ICS/MCS with a fixed amount of ICS. The breakthrough time of arsenic was quite similar in the different ratios ICS/MCS. However, much slower breakthrough of arsenic was observed as the ratio of ICS/MCS decreased. As the ratio of ICS/MCS decreased the concentration of As(III) in the effluent decreased and then showed below 50 ppb at an equal amount of ICS and MCS, suggesting more efficient oxidation of As(III) by greater amount of MCS. When a complete breakthrough of arsenic occurred, the removed total arsenic with an equal amount of ICS and MCS was 68.5 mg with 1 kg of filter material.

Studies on the Varietal Features for the Silk Yielding Ability, Bombyx mori L. (가잠의 견사물질 생성능력에 있어서 품종간의 특이성에 관한 연구)

  • 이용우
    • Journal of Sericultural and Entomological Science
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    • v.22 no.1
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    • pp.26-45
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    • 1980
  • Silk protein is synthesized in the silkgland of silkworm, Bombyx mori L. It is evident that silk productivity is one of the high heritable characters from the genetical aspects. It is also changed with the environmental circumstances. With this regard, this paper dealt with the varietal patterns of silkgland development and the factors concerning to the silk productivity of silkgland of silkworm by the synthesis of nucleic acids, profiles of amino acids and histological basis, using the eight parent silkworm varieties and their F$_1$ hybrids. 1. The weight of silkgland per larva increased proportionally in the F$_1$ hybrids which were crossed between longer silk yielding varieties. The silk content to the weight of the silkgland was higher in the longer silk yielding varieties than that in the shorter silk yielding varieties. 2. It was observed that the morphological changes of nuclei took place in the posterior silkgland cells with the larval development of the 5th instar. In varietal aspect, Jam 107 and Jam 108, longer silk yielding varieties, showed more branches in nuclei than the $N_2$ and $C_{60}$ which were shorter yielding ones. 3. It was observed that there was a high correlation between RNA content per unit weight of silkgland on the 6th day stage of 5th instar and silk productivity both in the parents and their F$_1$ hybrids. 4. RNA and DNA synthesis brought about thirty percent increase in the posterior silkgland of the longer silk yielding varieties during the 2nd day to the 4th day stages of the 5th instar, when compared with those in the posterior silkgland of the shorter silk yielding varieties. 5. RNA/DNA ratio in the posterior silkgland on the 2nd day and 4th day stages of the 5th instar was more increased in the longer silk yielding varieties than the shorter silk yielding varieties. 6. It was shown that DNA content for the longer silk yielding varieties came to be 374$\mu\textrm{g}$ per larva in the posterior division of silkgland on the 4th day stage of 5th instar, whereas it was 199$\mu\textrm{g}$ per larva for the shorter silk yielding varieties. 7 There was 34.8% Alanine, 22.8% Glycine, 9.1% Serine and 7.3% Tyrosine in the posterior division of silkgland as major amino acids. It is noticed that there was a little differences between the amino acids composition of posterior silkgland and silk fibroin. 8. There was some differences in the amino acids composition of posterior silkgland between pure lines and their hybrids. Glycine, Serine and acidic amino acids, essential to silk formation, seemed to be increased in the F$_1$ hybrids, whereas other amino acids such as Valine, Iso-leucine, Leucine, Lysine. Phenylalanine, Histidine and Arginine were reduced. 9. The content of Glycine, Alanine and Serine in the posterior division of silkgland was elevated in the longer silk yielding varieties than the others. Consequently. these three amino acids in the posterior silkgland seemed to be related to the silk yielding ability in the longer silk yielding varieties.s.

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A Study on Development of Technology System for Deep-Sea Unmanned Underwater Robot of S. Korea analysed by the Application of Scenario Planning (한국형 수중로봇시스템의 기술개발연구 - 시나리오플래닝 적용으로 -)

  • Lee, Sang-Yun;Yoon, Hong-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.8 no.1
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    • pp.27-40
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    • 2013
  • This study is about development of technology system for an advanced deep-sea unmanned underwater robot of S. Korea analysed by the application of scenario planning. It was developed a 6000m class next-generation deep-sea unmanned underwater vehicle(or robot, UUV) system, soonly ROV 'Hemire' and Depressor 'Henuvy' in 2006 at S. Korea and motion control, adaptive control algolithm, a work-space manipulator control algolithm, especially the underwater inertial-acoustic navigation system robust to initial errors and sensor failures. But there are remained matters on position tracking of the USBL, inertial-acoustic navigation system, attitude sensor, designed sonar sensors. So this study suggest the new idea for settle the matters and then this idea help the development of the underwater inertial-acoustic navigation system robust to initial errors and sensor failures, such as acoustic signal drop-out, by modifying the error covariance of the failed sonar signal when drop-out occurs. As a result, the future policy for deep-sea unmanned underwater robot of S. Korea is to further spur the development of new technology and more improvement of the technology level for deep-sea unmanned underwater robot system with indicator and imaginary wall as external device.

Characteristics of Velocity Fields around 3-Dimensional Permeable Submerged Breakwaters under the Conditions of Salient Formation (설상사주 형성조건 하에 있는 3차원투과성잠제 주변에서 내부유속변동의 특성)

  • Lee, Kwang-Ho;Bae, Ju-Hyun;An, Sung-Wook;Kim, Do-Sam
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.29 no.6
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    • pp.399-409
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    • 2017
  • This study numerically investigates the characteristics of the velocity field including the average flow velocity, longshore current and turbulent kinetic energy acting as the main external forces of the salient formed behind the permeable submerged breakwaters. Shoreline response is also predicted by the longshore-induced flux. In this paper, a three-dimensional numerical wave tank based on the OLAFOAM, CFD open source code, is utilized to simulate the velocity field around permeable submerged breakwaters under the formation condition of salient. The characteristics of the velocity field around permeable submerged breakwaters with respect to the gap width between breakwaters and the installing position away from the shoreline under a range of regular waves for different wave height are evaluated. The numerical results revealed that as the gap width between breakwaters increases, the longshore currents become stronger. Furthermore, as the gap width becomes narrower, the point where flow converges moves from the center of the breakwater to the head part. As a result, it is possible to understand the formation of the salient formed behind the submerged breakwaters. In addition, it was found that the longshore currents caused by the gap width between breakwaters and the installation position away from the shoreline are closely related to the turbulent kinetic energy.

Characteristics of Water Surface Variations around 3-Dimensional Permeable Submerged Breakwaters under the Conditions of Salient Formation (설상사주 형성조건하에 있는 3차원투과성잠제 주변에서 수면변동의 특성)

  • Lee, Kwang-Ho;Bae, Ju-Hyun;An, Sung-Wook;Kim, Do-Sam
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.29 no.6
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    • pp.335-349
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    • 2017
  • Submerged breakwaters installed under the water surface are a representative coastal structure to prevent coastal erosion, and various types of submerged breakwaters have been proposed and discussed so far. Generally, submerged breakwaters make the complex wave fields due to abrupt change in water depth at the crown of the breakwater. In this study, wave heights and mean water level formed around a breakwater are examined numerically for three-dimensional permeable submerged breakwaters. OLAFOAM, CFD open source code, is applied in the numerical analysis, and the comparisons are made with available experimental results on the permeable upright wall and the impermeable submerged breakwater to verify its applicability to the three-dimensional numerical analysis. Based on the applicability of OLAFOAM numerical code, the wave height and mean water level distribution formed around the permeable submerged breakwaters are investigated under the formation condition of salient. The numerical results show that as the gap width between breakwaters decreases, the wave height in the center of the gap increases, while it decreases behind the gap, and the installing position of the breakwater from the shoreline has little influence on the change of the wave height. Furthermore, it is found that the decrease of the mean water level near the gap between breakwaters increases with decreasing of the gap width.

Detailed Bathymetry and Seabed Characteristics of Wangdol-cho, Hupo Bank in the East Sea (동해 후포퇴 왕돌초 주변의 정밀해저지형 및 해저면 특성 분석)

  • Kim, Chang Hwan;Park, Chan Hong
    • Economic and Environmental Geology
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    • v.47 no.5
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    • pp.533-540
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    • 2014
  • The Wangdol-cho area, in the Hupo Bank, plays a very important role in main fishing grounds, leisure tourism and marine environmental researches of the East Sea. We analyzed the detailed bathymetry and classified the seabed characteristics of the Wangdol-cho area, based on seafloor backscattering images and sediment grain size. The Hupo Bank is developed in parallel with the eastern coastal line of Korean peninsula, and the shallowest area (Wangdol-cho) of the Hupo Bank is located along the eastern part of Hupo Port. The Wangdol-cho comprises three summits; north summit, middle summit, and south summit. The middle summit area among the three summits has the most shallow water depth with minimum about 6 m. The north summit shows about 8 m minimum depth and the south summit about 9 m. The bathymetry data around three summits represent undulating seabeds with many scattered underwater reefs and shallow water depth. The area between the underwater reefs, the flat seafloor in the northeastern part of the survey site, and the western steep slope area have relatively coarse sediments such as sandy gravel and gravelly sand. The bathymetry in the western side of the Wangdol-cho shows steep slope seabed, extending to the Hupo Basin. Fine sediments including mud and silty sand occur in the Hupo Basin area of the survey site. The submarine detailed topography and the analysis of the seafloor characteristics of the survey area are expected to contribute to management for marine environmental researches and sustainable use of ecosystems in the Wangdol-cho.

POSTSEDATION EVENTS IN PEDIATRIC PATIENTS SEDATED FOR DENTAL TREATMENT (진정법을 이용한 치과 치료 시 소아 환자의 진정 후 반응에 대한 연구)

  • Koo, Jung-Eun;Baek, Kwang-Woo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.36 no.2
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    • pp.209-216
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    • 2009
  • The purpose of this retrospective study was to examine twenty four hour postsedation events after discharge in children sedated for dental treatment. The sedation sheets of 355 children were selected. Selection criteria excluded no midazolam or nasal route of midazolam, no ASA class I, absent or incomplete questionnaire. Children received orally chloral hydrate and hydroxyzine, nitrous oxide was maintained during the sedation. Midazolam was injected to buccal submucosa with titration. Parents were interviewed via telephone within twenty four hours after sedation. Questionnaire included sleeping response, sleeping time, sleeping behavior, vomiting response at home. Two hundred twenty four (67.8%) children slept and twenty one(6.3%) children had abnormal sleeping behavior. One hundred four(31.0%) children had abnormal behavior after sleep and ten(3.0%) children had vomiting response. In the children took the higher dose of midazolam, there were the great tendency to sleep after discharge (p<0.05), the more negative response to sleeping behavior(p = 0.055), the longer sleeping time(p=0.054). In this study many children slept after sedation and a few children showed negative response. However there were not any considerable emergency events. Further study is required to determine the postsedation safety for the use of submucosal midazolam combination with chloral hydrate and hydroxyzine.

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Efficient Inoculation Method of Beauveria bassiana for Production of Bombycis corpus and Evaluation of Its Liver Protection Activity (백강잠(白彊蠶) 생산을 위한 Beauveria bassiana의 효율적인 접종법 및 백장잠의 간보호 활성 검정)

  • Jung, I-Yeon;Hong, In-Pyo;Kang, Pil-Don;Nam, Sung-Hee;Kim, Mi-Ja
    • Journal of Sericultural and Entomological Science
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    • v.47 no.1
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    • pp.5-11
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    • 2005
  • When inoculating with B. bassiana 101A for the mass production of B. corpus, the infection ratio was high with regardless of the treating time with highly-humidity if the concentration of spore was 1.0${\times}$$10^8$ spores/m/, but that was low if the concentration was 1.0${\times}$$10^7$ spores/m/. In the study of the activities according to the coserving temperature or days of the B. bassiana spawn, the infection ratio of 90% was maintained for 12 days in the temperature of $4^{\circ}C$. However, the infection ratio was rapidly dropped to the below of 5% after conserved for 48 hours in the temperature of $25^{\circ}C$. Besides, the activities of the original isolate had no difference after conserved for 12 months in the temperature of $4^{\circ}C$, so that the infection ratio 90% could be mintatined. In the measure of liver-protecting activities of B. bassiana 101A, the recovering effect was 43.5% and 65.7% respectively in the poisonous treatment induced with galactosamine, compared with liver-protecting activities of Silymarin and DDB in the $H_2O$ fraction. In the poisonous treatment induced with $CCl_4$ the recovering effect was 100% and 69.3% respectively, compared with liver-protecting activities of Silymarin and DDB in the EtOAc fraction.

The Effect of Organic solvents on Interrupting Diapause Initiation in Prospective Diapause Eggs of Bombyx mori. (가잠란의 휴면개시조지에 관한 유기용매의 효과)

  • 김삼은
    • Journal of Sericultural and Entomological Science
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    • v.27 no.1
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    • pp.24-30
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    • 1985
  • The effect of some organic solvents on interrupting diapause of Bombyx mori eggs was examined to provide a clue to the mechanism of diapause initiation. Methyl alcohol and benzene proved to be efficient in developing the prospective diapause eggs upto the stage of hatching or body pigmentation of the embryos. On the other hand, most of the eggs soaked in chloroform and mixture solution of chloroform-methyl alcohol (2:1) died in the early embryonic developmental stage with yellow or red brown colours, and the egg weights decreased upto ca. 40% of the original weight 8 days after the treatments. Methyl alcohol treatment for 2, 5 and 10 min to the 5hr-old-eggs led to empergence of the larvae, with high incidence (70∼80%) in the race of Kumchu X Chonghwa and with low indidence (1∼4%) in Daezo. The effect of same treatment to 20hr old eggs decreased to ca. 10% in the emergence of larvae in Kumchn X Chonghwa and increased to 20∼30% in Daezo, while the effect disappeared shortly after the diapause initiation (48hr-old-eggs). Considering the high dependency upon the egg age of the sensitivity to solvents, it was supposed that initiation and termination of diapause may be controlled by different mechanism. It was also suspected that the solvents exert their effect on the permeability of the eggshell.

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