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Efficient Inoculation Method of Beauveria bassiana for Production of Bombycis corpus and Evaluation of Its Liver Protection Activity  

Jung, I-Yeon (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology)
Hong, In-Pyo (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology)
Kang, Pil-Don (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology)
Nam, Sung-Hee (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology)
Kim, Mi-Ja (Department of Agricultural Biology, The National Institute of Agricultural Science and Technology)
Publication Information
Journal of Sericultural and Entomological Science / v.47, no.1, 2005 , pp. 5-11 More about this Journal
Abstract
When inoculating with B. bassiana 101A for the mass production of B. corpus, the infection ratio was high with regardless of the treating time with highly-humidity if the concentration of spore was 1.0${\times}$$10^8$ spores/m/, but that was low if the concentration was 1.0${\times}$$10^7$ spores/m/. In the study of the activities according to the coserving temperature or days of the B. bassiana spawn, the infection ratio of 90% was maintained for 12 days in the temperature of $4^{\circ}C$. However, the infection ratio was rapidly dropped to the below of 5% after conserved for 48 hours in the temperature of $25^{\circ}C$. Besides, the activities of the original isolate had no difference after conserved for 12 months in the temperature of $4^{\circ}C$, so that the infection ratio 90% could be mintatined. In the measure of liver-protecting activities of B. bassiana 101A, the recovering effect was 43.5% and 65.7% respectively in the poisonous treatment induced with galactosamine, compared with liver-protecting activities of Silymarin and DDB in the $H_2O$ fraction. In the poisonous treatment induced with $CCl_4$ the recovering effect was 100% and 69.3% respectively, compared with liver-protecting activities of Silymarin and DDB in the EtOAc fraction.
Keywords
Bombyx; mori; Silymarin; B. bassiana;
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