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Development of hovering-type AUV test-bed 'OCTAGON' (호버링 타입 자율무인잠수정 'OCTAGON'의 테스트베드 개발)

  • Choi, Dong-Ho;Lee, Young-Jin;Hong, Sung-Min;Kim, Joon-Young
    • Journal of Advanced Marine Engineering and Technology
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    • v.40 no.6
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    • pp.516-526
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    • 2016
  • This paper introduces a hovering-type autonomous underwater vehicle (AUV) developed for research and its fundamental motion performance results obtained by simulation and field test. The AUV can control its motion in four degrees of freedom (DOF) by means of its horizontal and vertical thrusters, and it is designed to provide a test-bed that facilitates ease of operation and experimentation. Prior to the field tests, six DOF equations of motion are developed, and a simulation program is constructed using MATLAB and Simulink to verify the essential motion performance of the designed vehicle. Furthermore, a proportional-integral-derivative (PID) controller and fuzzy PID controller are designed, and their performances are verified through a simulation. Field tests are performed to verify the motion performance of the AUV; way-point tracking is executed by the PID and fuzzy PID controllers. The results confirmed appropriate control performance under current disturbances.

Comparative Studies of Digestive Fluid Protein of Silkworm Bombyx mori, Larvae reared on Mulberry Leaves and Artificial Diets (상엽육잠과 인공사료육잠의 소화액단백질의 비교연구 - 소화액 RFP를 중심으로 -)

  • 박희정;문재유
    • Journal of Sericultural and Entomological Science
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    • v.28 no.1
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    • pp.15-23
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    • 1986
  • This study has been carried out to acquire some basic informations on the digestive fluid of Silkworm, Bombyx mori for developing artficial diets. Silkworms reared on mulberry leaves and artificial diet, were used in this experiments. The results obtained are as follows ; 1. The Red Fluorescent protein was precipitated in 50% acetone solution and did not dissolved in n-butanol solution, but dissolved in methanol solution. 2. Electrophoretic analysis results of mulberry leaves rearing B. mori and artificial diet rearing B. mori, which has been treated with 50% acetone solution were as follows. i) There was distinct difference at the position of high mobility ii) Red Fluorescence was observed only at the position of first band of mulberry leaves rearing B. mori. iii) No different was shown in the electrophoretic patterns of mulberry leaves rearing B. mori on 5th instar 1st-3rd day, but some difference on 5th instar 4th, 5th day. 3. The RFP is the basic protein which has PI 8-9 according to the isoelectric electrophhoresis. 4. The SDS-polyacrylamide gel electrophoresis analysis showed that the molecular weight of RFP was 27,000. 5. The Sephadex G-75 chromatographic analysis showed that there was three peaks between number 16 and 28 in the mulberry leaves rearing B. mori chromatogram, but two peaks between number 18 and 31 in the artifical diet rearing B. mori chromatogram.

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Structure and mechanical properties of Korean commercial silkworm cocoon (장려품종 누에고치의 구조 및 기계적 특성)

  • Kim, Sung-Kuk;Jo, You-Young;Lee, Kwang-Gill;Kim, Kee-Young;Kim, Hyun-bok;Kweon, HaeYong
    • Journal of Sericultural and Entomological Science
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    • v.53 no.2
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    • pp.97-100
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    • 2015
  • Korean commercial Bombyx mori silkworm cocoon made by Kumokjam and Daesungjam was examined to characterize for application. Amino acid analysis showed that the main amino acid of Kumokjam and Daesungjam cocoon was glycine, alanine, serine, tyrosine, and aspartic acid. The content of three major amino acids was about 67% and the content of long chain amino acids was about 37%. Stress-strain curve showed that breaking stress of Kumokjam cocoon, 50 MPa, was higher than that of Daesungjam cocoon, 48 MPa. Breaking strain of Kumokjam cocoon, 37%, was also longer than that of Daesungjam cocoon, 30%. These results might be used as basic information for development of non-textile materials using Bombyx mori silkworm cocoon.

Simultaneous Production System of Silkworm Dongchunghacho and Male Pupae Using Both Parent Sex-limited Larval Marking Variety (한성반문잠품종을 이용한 누에동충하초 및 숫번데기의 동시 생산체계)

  • Ji, Sang-Duk;Kim, Nam-Suk;Kang, Pil-Don;Sung, Gyoo-Byung;Hong, In-Pyo;Ryu, Kang Sun;Kim, Young-Ki;Nam, Sung-Hee;Kim, Mi-Ja;Kim, Kee-Young
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.101-108
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    • 2012
  • This study was conducted to confirm the mass production of male pupae and sex-limited larval marking variety as a host for synnemata production of Isaria tenupes in RDA(Rural Development Administration). Silkworm pupation, infection rate and synnemate formation of I.tenuipes were examined. Among the silkworm varieties tested, male Hansaengjam showed the highest pupation rate at 98.7%. I. tenuipes infection rate of larvae of newly-exuviated 5th instar silkworm was 83.7 ~ 90.4% in the spring rearing season and 91.7 ~ 96.6% in the autumn rearing season. Synnemata production of I. tenuipes was execellent in female Yangwonjam with an incidence rate of 99.5% followed by male Yangwonjam(99.5%) and Baegokjam(99.4%) in the spring and autumn rearing season. Synnemata living weight ranged from 0.93 ~ 1.25 g in the spring rearing season. The female Hansaengjam had the heaviest synnemata weight(1.25 g). Synnemata dry weight ranged from 0.27 ~ 0.35 g in the spring rearing season. The female Yangwonjam had the heaviest synnemata weight(0.35 g).

Identification of Domesticated Silkworm Varieties Using a Whole Genome Single Nucleotide Polymorphisms-based Decision Tree (전장유전체 SNP 기반 decision tree를 이용한 누에 품종 판별)

  • Park, Jong Woo;Park, Jeong Sun;Jeong, Chan Young;Kwon, Hyeok Gyu;Kang, Sang Kuk;Kim, Seong-Wan;Kim, Nam-Suk;Kim, Kee Young;Kim, Iksoo
    • Journal of Life Science
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    • v.32 no.12
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    • pp.947-955
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    • 2022
  • Silkworms, which have recently shown promise as functional health foods, show functional differences between varieties; therefore, the need for variety identification is emerging. In this study, we analyzed the whole silkworm genome to identify 10 unique silkworm varieties (Baekhwang, Baekok, Daebaek, Daebak, Daehwang, Goldensilk, Hansaeng, Joohwang, Kumkang, and Kumok) using single nucleotide polymorphisms (SNP) present in the genome as biomarkers. In addition, nine SNPs were selected to discriminate between varieties by selecting SNPs specific to each variety. We subsequently created a decision tree capable of cross-verifying each variety and classifying the varieties through sequential analysis. Restriction fragment length polymorphism (RFLP) was used for SNP867 and SNP9183 to differentiate between the varieties of Daehwang and Goldensilk and between Kumkang and Daebak, respectively. A tetra-primer amplification refractory (T-ARMS) mutation was used to analyze the remaining SNPs. As a result, we could isolate the same group or select an individual variety using the nine unique SNPs from SNP780 to SNP9183. Furthermore, nucleotide sequence analysis for the region confirmed that the alleles were identical. In conclusion, our results show that combining SNP analysis of the whole silkworm genome with the decision tree is of high value as a discriminative marker for classifying silkworm varieties.