• Analytical Science and Technology
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• v.34 no.3
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• pp.122-127
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• 2021

A mixture of polyvinylpyrrolidone and 1,2-indanedione (PVP-IND) is known to be the best reagent for developing latent fingerprints deposited on the surface of a thermal paper. However, no study has evaluated whether PVP-IND could enhance fingerprints in blood deposited on the surface of thermal paper. Therefore, in this study, the ability of PVP-IND to enhance the fingerprints in blood was investigated. Furthermore, aqueous amido black solution, which is known to be the best reagent for enhancing fingerprints in blood deposited on the surface of thermal paper, was used for comparison with PVP-IND. Therefore, while enhancing the fingerprints in diluted blood, PVP-IND enhanced the quality of fingerprints compared to aqueous amido black solution. The ridge diffused when the undiluted fingerprints in blood were enhanced with PVP-IND because PVP reacted with amino acids in blood; however, amido black solution yielded better results. When treated with PVP-IND, it is presumed that the ridge diffused due to the reaction of PVP and amino acids contained in the blood.

• Analytical Science and Technology
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• v.30 no.5
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• pp.270-278
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• 2017

The absorption of blood onto the surfaces of white cotton, polyester, rayon, and nylon fabrics was studied. Different categories of fabrics (woven and knitted) with diverse thickness, were manually folded twice to obtain four fabric layers, and $100{\mu}L$ of human blood was dropped onto the surface of the fabrics. The amount of blood that penetrated the fabric layer and the shape of bloodstain observed on the fabrics were influenced by the chemistry, thickness, and texture of the fabric. The blood bearing fabrics were left to dry for 3 days, washed by hand using tap water, and Lumiscene was then sprayed onto the fabrics to enhance the latent bloodstain for comparison of the shape of the bloodstain before and after washing by hand. The features of the bloodstain after washing varied greatly with the recipient fabrics. Additionally, stronger luminescence was observed at the surface where the blood was deposited compared to the background. However, it was confirmed that physical contact during the washing can deform the original shape of the bloodstain. The effect of the drying time on the bloodstain after hand washing was also studied. $100{\mu}L$ of blood was dropped on the surfaces of the fabrics and dried for 0, 1, 12, 24, 72 h, and 7 days, then washed by hand, before the bloodstain was enhanced with Lumiscene. The results of this experiment indicated that the increased drying time induces stronger chemiluminescence of Lumiscene. However, after drying of the bloodstain for 7 days, the luminescence of the bloodstain was decreased at the blood deposited site and increased around the blood deposited site.

• Journal of the Korea Convergence Society
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• v.11 no.10
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• pp.139-146
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• 2020

Kastle-Meyer (KM) test is one of presumptive tests used to detect latent blood in crime scenes. While this method is more sensitive than others, false positive reaction can be shown where blood does not exist. In this study, we tried to suggest a new application method to solve this problem. Reaction time and aspect of reaction of this new method was compared with two conventional methods in order to identify the effectiveness and identifiability. As a result, there was no statistically significant difference in sensitivity between the methods. In addition, in case of blood with a dilution ratio of 5000:1 or less, positive reaction showed immediately, making it easy to distinguish the reaction from false positive reaction. However, it became difficult to distinguish them with the reaction time as the dilution ratio increased, and this phenomenon could be supplemented observing aspect of the reaction when using the new method. Therefore, this study suggested a new method for KM test that can be used more accurately in the field.

• The Journal of the Korea Contents Association
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• v.16 no.6
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• pp.474-480
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• 2016

Bloody fingerprints are important in many cases - especially in murder case. This study compared effectiveness of some reagents which had better quality of developing bloody fingerprints from human skin. Leuco crystal violet(LCV), hungarian red, amido black, coomassie blue, acid violet17, and crowle's double stain were used. Fingerprints were generated in the depletion series, and tissue method was used in applying all reagents. LCV showed the best result in this study.