• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.35 no.2
• /
• pp.146-150
• /
• 1990

This study was conducted to investigate the genetic analysis of esterase isozymes in maize with tillers. The materials used for the study were stele tissue for five day old seedlings of IK inbred line with tiller and A-type inbred line with no tiller, their Fl and F,. The methods employed for the study were same as previous report by Lee and Choe. A total of thirteen isoesterase enzyme bands were identified and five zones were distinguised according to both migration distance and genetic segregation patterns. The E$\sub$0.3/, E$\sub$0.4/ and E$\sub$0.5/ loci appeared from orgin to 0.5cm migration distance were controlled by the two alleles in (IK/A-type)F$_2$ and the E$\sub$0.3/+E$\sub$0.4/ of variants was controlled by codominant alleles. The E$\sub$1.0/, E$\sub$1.2/, E$\sub$1.5/ and E$\sub$1.8/ loci appeared from 1.0cm to 1.8cm were also controlled by the two alleles. However, the null band was functioned alleles. The E$\sub$2.8/, E$\sub$3.0/ and E$\sub$3.5/ loci appeared from 2.8cm to 3.5cm migration distance were very active and near location. A total of individuals with two paried bands of these loci were more than those of three paired bands(x$^2$=0.327$\^$**/). The activity of bands appeared over 3.8cm were very low and these were controlled by the two alleles. In above results, genetic segregations of stele tissue of maize with tillers were suggested to be controlled by Mendelian genetic laws.

• Food Engineering Progress
• /
• v.13 no.4
• /
• pp.243-250
• /
• 2009

Pravastatin sodium, competitive inhibitors of HMG-CoA(3-hydroxy-3-methylglutaryl coenzyme A) reductase, is produced from the culture broth of Streptomyces carbophilus KCCM 10370, The production of Pravastatin sodium was increased about 45 fold compared to wild type by UV mutation. Production of Pravastatin was also improved by continuous feeding of Compactin sodium to 24% and bioconversion ratio was also increased to 4.3% by intermittent addition. In main culture, concentration of Compactin sodium was kept less than 0.1%(w/v) under continuous feeding of Compactin sodium then product was 0.49% and bioconversion was 70%. After finishing the fermentation, Pravastatin was purified by various chromatographies such as Diaion HP20 resin column, Partition, and ODS(Octa-Decylsilyl Silicagel) resin column with a final yield of 70~72% and over 99.7% purity. The IR, UV, and NMR study of the purified Pravastatin sodium showed the same pattern as that of EP(European Pharmacopoeia).

• Journal of the Korean Society for Railway
• /
• v.14 no.3
• /
• pp.248-255
• /
• 2011

Impact factor of concrete and ballast track which has been used in Korea railway was applied to equation (1+0.513V/100) from AREA. As the use of this equation, overcapacity of track design might be occurred. Therefore, this study compared impact of ballast track (well, bad) and concrete track (sleeper embeded system, rail floating and sleeper floating) by field test to analyzing dynamic effect of track structure's characterstic and wheel load on service line. In addition, it suggested a method to generate reasonable impact factor on each track type.

• Journal of the Earthquake Engineering Society of Korea
• /
• v.11 no.1 s.53
• /
• pp.67-77
• /
• 2007

Site coefficients in IBC and KBC codes have some limits to predict the rational seismic responses of a structure, because they take into account only the effect of the soil amplification without the effects of the structure-soil interaction. In this study, upper and lower limits of the site coefficients are estimated through the pseudo 3-D elastic seismic response analyses of structures built on the linear or nonlinear soil layers taking Into account the effects of the structure-soil interaction. Soil characteristics of site classes of A, B and C were assumed to be linear, and those of site classes of D and E were done to be nonlinear and the Ramberg-Osgood model was used to evaluate shear modulus and damping ratio of a soil layer depending on the shear wave velocity of the soil layer, Seismic analyses were performed with 12 weak or moderate earthquake records scaled the peak acceleration to 0.1g or 0.2g and deconvoluted as earthquake records at the bedrock located at 30m deep under the outcrop. With the study results of the elastic seismic response analyses of structures, new standard response spectrum and upper and lower limits of the site coefficients of $F_{a}\;and\;F_{v}$ at the short period range and the period of 1 second are suggested including the effects of the structure-soil interaction, and new site coefficients for the KBC code are also suggested.

• KSBB Journal
• /
• v.5 no.3
• /
• pp.279-291
• /
• 1990

Partial hydrolysed and deacetylated chitin, CHITA and CHITB as supports of immobilized enzyme were obtained by treatment of acid and base respectively. Glutaraldehyde, bifunctional reagent, was employed for crosslinking between $\beta$-glucosidase and support. Immobilized enzyme activities of CHITA-Gase and CHITB-Gase were determined with the reaction of p-nitrophenol-$\beta$-D-glucopyranoside(PNG) in batch reactor, CSTR and PFR. Their optimum temperature, pH and enzymatic characteristics including Km and Vmax values were observed with variation of the flow rates. Mass transfer coefficient(h), effectiveness factor(η), deactivation rate(kd ) of two immobilized enzymes were also examined to compare efficiency of reactors.

• Food Science and Preservation
• /
• v.16 no.3
• /
• pp.376-384
• /
• 2009

Response surface methodology (RSM) was used to monitor the characteristics of ethanol extracts from glasswort (Salicornia herbacea). A central composite design was used to investigate the effects of the independent variables of sample ratio, extraction temperature, and ethanol concentration on the dependent variables color, sugar, salinity, yield, electron donating ability, and total polyphenol content of extracts. The maximum $^{\circ}Brix$ (8.46) was obtained under specific extraction conditions, with a sample ratio of 7.04 g/100 mL, an extraction temperature of $89.01^{\circ}C$, and an ethanol concentration of 34.29% v/v. At a sample ratio, extraction temperature, and ethanol concentration of 7.00 g/100 mL, $89.15^{\circ}C$, and 34.14% v/v, respectively, the salinity was 7.35%. When the sample ratio, extraction temperature, and ethanol concentration were 5.56 g/100 mL, $68.61^{\circ}C$, and 99.14% v/v, respectively, the maximum electron donating ability was 86.10%. A maximized total polyphenol content of 1,140.15 mg/100 g was found with the following conditions: sample ratio of 8.6 g/100 mL, extraction temperature of $64.19^{\circ}C$, and ethanol concentration of 71.74% v/v. Overall, the optimal ranges of extraction conditions for effective components of glasswort were 3.38.5.33 g/100 mL sample ratio, $55.87-76.96^{\circ}C$, and 25.00.67.31% v/v ethanol.

• Journal of Korean Society of Forest Science
• /
• v.104 no.3
• /
• pp.383-389
• /
• 2015

The purpose of this study was to investigate the anti-apoptosis-related protein expression in skeletal muscle of rats with different amount of mountain ginseng (MG) added high-fat diet fed. Twenty-four Sprague Dawley male rats were divided into the high-fat diet control group (CON), 0.5% of MG added diet group (MG1), and 1.0% of MG added diet group (MG2) with eight rats each. The P53, anti-apoptotic protein, was significantly lower in MG2 than CON and MG1. The bcl-2 and bcl-xl, however, were not significantly different from MG1 but from CON. The caspase-9 and -3, were significantly lower in MG groups than CON. In addition, it was dramatically lower in MG2. These results suggested that MG addition to the high-fat diet suppressed p53 protein expression and enhanced anti-apoptototic protein expression. MG may be a positive effects on health as a medicinal plant.

• Journal of the Mineralogical Society of Korea
• /
• v.22 no.4
• /
• pp.307-312
• /
• 2009

Motukoreaite and quintinite-3T, Mg-Al layered double hydroxides, were found in the Sinyangri Formation of Jeju Island. They fill the pores of basaltic volcaniclastic sediments in globular and botryoidal aggregates of fine platy particles. Globular aggregates of quintinite-3T were crusted with the parallel overgrowth of motukoreaite plates. X-ray diffraction data and chemical composition are consistent with those reported in literature, while the Mg/Al ratio of motukoreaite is higher. Structural formula of motukoreaite and quintinite-3T derived from electron microprobe analysis are $Na_{1.6}Ca_{0.1}Mg_{40.7}Al_{20.7}Si_{0.9}(CO_3)_{13.6}(SO_4)_{7.4}(OH)_{108}56H_2O$, and $Mg_{3.7}Al_{1.9}Si_{0.2}(OH)_{12}(CO_3)_{0.8}(SO_4)_{0.2}3H_2O$, respectively. Motukoreaite and quintinite-3T were formed by reaction between seawater and basaltic glass, and contributed to the cementation and lithification of the volcaniclastic sediments.

• Journal of the Mineralogical Society of Korea
• /
• v.4 no.1
• /
• pp.1-10
• /
• 1991

Tosudite from the Sungsan mine, Korea is an alteration product of rhyolitic tuff in the cretaceous Hwangsan Formation. It is associated with illite, dickite, nacrite or quartz and also found in the cavities of black claystone. X-ray diffraction and chemical analyses show that the Sungsan tosudite is a lithium-bearing aluminous 1:1 regularly interstratified mineral of di, dioctahedral chlorite and smectite. Its structural formula is $(K_{0.73}Na_{0.02}Ca_{0.07})(Si1_{13.23}Al_{2.77})(Li_{0.52}Mg_{0.08}Mn_{0.01)Fe^{3+}_{0.07}Al_{12.33})O_{40}(OH)_{20}$ and it suggests that Sungsan tosudite consists of regularly interstratified Li-donbassite and beidellite. DTA and TG curves as well as IR absorption data also support such a result. Temperature of formation of tosudite is inferred to be between $110{\circ}$ and $270{\circ}C$.

• Proceedings of the Korean Aquaculture Society Conference
• /
• 2003.10a
• /
• pp.151-152
• /
• 2003

물리ㆍ화학적 및 효소적 방법을 이용한 근육의 품질판정 지표로써 수분함량, 지질함량 그리고 콜라겐 함량등을 살펴보았으며, AEC 수치로써 건강도를 측정하고자 하였다. 즉, 이런 지표들을 이용하여 양식산 활어의 품질에 가장 큰 요인으로 작용하는 파괴강도와의 상관관계를 살펴보고자 하였다. 지금까지 조사된 양식산 활어성분 중 파괴강도가 어떠한 독립변수들에 의하여 크게 영향을 받으며, 파괴강도와 독립변수와의 관계식은 무엇인가를 규명하기 위하여 회귀분석을 실시하였다 여기서 독립변수로 사용된 것은 아래와 같다. X$_1$ : 수분함량(moisture content) X$_2$ : 지질함량(lipid content) X$_3$ : 콜라겐 함량(collagen content) 각 성분간의 상관관계를 피어슨 상관계수로 나타낸 것으로, 파괴강도와 수분 함량, 지질함량사이에는 상관성이 있으나(r=0.66, r=-0.69: p<0.05), 콜라겐의 함량과는 상관성을 가지지 못하였다. 또한, 콜라겐과 수분함량(r=0.19), 콜라겐과 지질함량(r=-0.29)로 각 성분간의 상관성이 존재하지 않았다. 그러므로, 파괴 강도값은 지질과 수분함량간의 상관관계가 형성된다. 즉, 수분함량이 크고 지질함량이 작아지면 파괴강도간은 커진다는 결론이다. 이 결과를 이용하여 파괴감도, 수분함량 그리고 지질함량간의 회귀분석을 실시한 결과 다음과 같다. Y = 0.74481 ＋ 0.01323 X$_1$- 0.05821 X$_2$ 이 회귀식을 이용하여, 참돔의 수분함량과 지질함량을 측정함으로써 참돔의 품질을 결정할수 있는 파괴강도값이 계산으로 얻어진다. 또한 효소적방법으로써 품질지표인 AEC수치를 측정함으로써 건강도를 판정할 수 있다. 그러므로, 파괴강도값을 이용하여 품질의 등급을 선정하며, AEC수치를 건강도의 등급을 선정하여야 된다. 파괴강도값에 의한 등급은 1.4kg이상은 상급, 1.2~l.4kg은 중급, 1.2kg이하를 하급으로 선정하며, AEC수치는 1.0~0.9는 상급, 0.9~0.8는 중급, 0.8이하를 하급으로 선정하여 등급화를 분류할 수 있을 것으로 판단된다.