In order to investigate the geographical variation of Glycine max distributed in southern area of Korean peninsula, 19 local strains(Goseng, Pyeongchang, Ganghwa, Pocheon, Geumsan, Seocheon, Jincheon, Danyang, Tongyeong, Sancheong, Gumneung, Wolseong, Woolneung, Wando, Naju, Gochang, Jangsu, Jeju, Bukjeju), which located from $33^{\circ}15'N$ to $38^{\circ}11'N$, were selected according to their latitudes and geographical distances. The seeds of these strains were collected and their contents of mannose and galactose were investigated. Mannose contents in the seeds were variable in the range between the highest 12.207mg/g(Gochang) and the lowest 6.648mg/g(Geumsan). The contents of galactose were represented remarkable differences from 9.967mg/g(Danyang) to 16.949mg/g(Bukjeju) also. The local strains were classified into 4 variation types such as the inland type I(Wolseong, Jangsu, Danyang), the coastal type I(Gochang, Naju, Jeju, Goseng, Woolneung, Bukjeju, Ganghwa, Seocheon, Tongyeong), the inland type II(Geumsan, Pocheon, Pyeongchang) and the coastal type II(Wando, Jincheon) and 2 strange strains(Gumneung, Sancheong) according to the geographical climatic type and the ratio of mannose/galactose, which indicate the hardness of seeds in Leguminosae, ranged from 0.63 to 0.78. The variation types are very significant genecologically as an evidence for microevolution related to speciation in cultivated plants.