• Reproductive and Developmental Biology
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• v.28 no.3
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• pp.203-207
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• 2004

The transgenic mice carrying human Interleukin-10 (hIL-10) gene in conjunction with bovine (3 -casein promoter express hIL-10 in milk during lactation. In this study, stability of germ line transmission and expression of hIL-10 transgene integrated into host chromosome were monitored up to generation F8 of transgenic mice. When male mouse of generation F8 was crossbred with normal females, approximately half of offspring (50.9±5.8%) were identified as transgenic mice. Generation F9 to F15 mice also showed similar transmission rates (66.0±20.1%, 61.5±16.7%, 41.1±8.4%, 40.7±20.3%, 61.3±10.8%, 49.2±18.8% and 43.8±25.9%, respectively), implying that hIL-10 transgene can be transmitted stably up to long term generation in the transgenic mice. Expression levels of human IL-10 from milk of generation F9 to F14 mice were 3.6± 1.2 mg/ml, 4.2±0.9 mg/ml, 5.7±1.5 mg/ml, 6.3±3.5 mg/ml, 6.8±4.5 mg/ml and 6.8±3.1 mg/ml, respectively, which was showed high-level expression compared with that of generation F1 (1.6 mg/ml) mice. In conclusion, our results suggest that transgenic mice can be continuously passed their transgenes to the progeny through the breeding program with the same productivity of human IL-10 protein in their milk.

• Journal of The Korean Association For Science Education
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• v.36 no.2
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• pp.203-219
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• 2016

The purpose of this paper was analyzing the contents of life science area in elementary science textbooks according to Korean science curricula change to get suggestions for the advancement of science curriculum. The framework of content analysis was developed by revising TIMSS 2015 life science evaluation framework. The results of this research were as follows: 'The differences of living things and non-living things appearing in the first grade mostly were not included in the 1st, the 6th and the 2009 revised curricula. Contents emphasizing rural life were appeared from 'Teaching themes period' to the 2nd curriculum period, disappeared from the 3rd curriculum period until the 2009 revised curriculum. Contagious disease was emphasized in all elementary grades in the 1st curriculum period, which reflected a social phenomena emphasizing health and hygiene after the Korean War. Mostly fungus was included until the 7th curriculum period and bacteria and virus were added from the 2007 revised curriculum period. The way of improving health was emphasized continuously.' The differences of living things and non-living things should be included in elementary science curriculum for the correct 'life' concept formation of elementary students. 'Strategies for helping the living of descendants' and 'the heredity of animal and plant' which were appeared in the lower grades, should be included at the higher grades with greater depth. The incoming elementary science textbooks also need to include science contents about evolution in greater depth, along with human health.

• The Korean Journal of Ecology
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• v.25 no.3 s.107
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• pp.163-170
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• 2002

In order to analyze the life cycle of A. artemisiifolia var. elatior dormancy and some environmental factors inducing germination of the seeds were examined. The results were as follows : Dormancy of fertile seeds was broken in part within a month after seed collection in case of adequate moisture and alternating temperature was also effective in breaking dormancy. The temperature range, which allow germination was $12^{\circ}C{\sim}32^{\circ}C$. Optimum temperature for germination was $24^{\circ}C$. The seed of A. artemisiifolia var. elatior was light-independent. The difference of storage period appeared to have no particular effect on the viability of seeds at any time during the 9-month storage period. In the increasing temperature(IT) regime, A. artemisiifolia var. elatior seeds started to germinate at $16^{\circ}C$, showing the higher temperature the greater germination rate : the final germination percentage was 99.34%. On the other hand, in the decresing temperature(DT) regime, seeds began to germinate at $20^{\circ}C$ with the 1.34% germination. An induced dormancy occurred at $12^{\circ}C$ making the 5.34% final germination in the DT regime. Low temperature was more effective to break dormancy than higher temperature. Seeds of A. artemisiifolia var. elatior seems to be germinated in mid to late autumn or germination delayed until following spring. The above results suggest these variation of germination response in diverse environmental factors seems to be a physiological strategy to maintain their existence and to reproduce in the extreme thermal variation.

• Journal of Life Science
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• v.28 no.4
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• pp.398-407
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• 2018

The Purple pericarp (Prp) trait is a trait often bred for in black rice. Generally, the Prp trait is displayed in the color variations of seeds following the 9:3:4 purple, brown, and white ratio, respectively. The Prp trait is a recessive epistasis of two gene interactions; however, it is caused by the two complementation genes Pb and Pp. Here we present a study of the genetic characteristics of the Prp trait using an $F_1$ hybrid with a Pbpb Pppp genotype. This hybrid generated four seed colors with the following numbers: 3 dark purple, 6 medium purple, 3 brown, and 4 white (or 9 purple, 3 brown, and 4 white). However, further biochemical analysis of the all progenies divided them into two groups. One group had the Pb_ Pp_ allelic constitutions and contained cyanidin 3-O-glucoside (C3G) in both the dark purple or medium purple seeds. The other group, however, was absent of C3G in both the brown and white seeds, resulting in a ratio of 9:7, respectively. This segregation revealed the extended Mendelian 9:7 ratios of the complementary gene interactions with a good fitness in ${\chi}^2$ analysis. Further analysis revealed that brown seeds with the Pb_ pppp genotype corresponded with a null C3G, indicating that the Brown pericarp trait in rice is caused by a dominant allele of the Pb gene. Therefore, we conclude that the production of C3G is a main phenotype of the black and purple colored rice in the Prp trait, and it is governed by the complementary gene interactions between Pb and Pp genes.

• Korean Journal of Poultry Science
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• v.18 no.1
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• pp.1-8
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• 1991

This study was conducted to estimate heritabilities and genetic correlations on body shape components in Korean Native Ogol fowl , The data analysis were record of 450 pullets producted from 150 dams and 20 sires of Korean Native Ogol fowl raised at Chungnam National University from June 18, 1987 to April 6, 1989. The results obtained are sammarized as follows ； 1. On the body components, shank length at 8, 24 weeks and 300 days of age were $8.717\pm$0.363, $9.576\pm$0.390 and $9.646\pm$0.389cm, the breast width were 3.469$\pm$0.234, $4.310\pm$0.345 and $4.752\pm$0.343cm； the breast girth at 8, 24weeks and 300 days of age were $22.819\pm$1.082, $36.719\pm$1.793 and $39.008\pm$1.709cm； the tibia length at 8, 24weeks and 300days of age were $9.941\pm$0.888, $13.183\pm$0.499 and $13.560\pm$0.552cm. 2. The heritability estimates of body shape components based on the variances of sires, dams and combined components from 8, 24 weeks and 300 days of age were 0.235-0.290, 0.589-0.890 and 0.437-0.565 for shank length ； 0.185-0.237, 0.444-0.536 and 0.314-0.392 for breast width ； 0.218-0.552, 0.499-0.746, 0.486-0.558 for breast girth ； 0.339-0.432, 0.281-0.543 and 0.340-0.446 for tibia length, respectively. 3. The genetic correlation coefficient of body shape components were as follows ； between shank length and breast width, breast girth, tibia length were 0.237-0.836, 0.277-0,729 and 0.378-0.915；between breast width and breast girth, tibia length were 0.637-0.889 and 0.384-0.903；between breast girth and tibia length was 0.905-0.995, respectively.

• Journal of Animal Science and Technology
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• v.53 no.1
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• pp.1-6
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• 2011

This simulation study was performed to investigate the accuracy of the estimated breeding value by using genomic information (GEBV) by way of Bayesian framework. Genomic information by way of single nucleotide polymorphism (SNP) from a chromosome with length of 100cM were simulated with different marker distance (0.1cM, 0.5cM), heritabilities (0.1, 0.5) and half sibs families (20 heads, 4 heads). For generating the simulated population in which animals were inferred to genomic polymorphism, we assumed that the number of quantitative trait loci (QTL) were equal with the number of no effect markers. The positions of markers and QTLs were located with even and scatter distances, respectively. The accuracies of estimated breeding values by way of indicating correlations between true and estimated breeding values were compared on several cases of marker distances, heritabilities and family sizes. The accuracies of breeding values on animals only having genomic information were 0.87 and 0.81 in marker distances of 0.1cM and 0.5cM, respectively. These accuracies were shown to be influenced by heritabilities (0.87 at $h^2$ =0.10, 0.94 at $h^2$ =0.50). According to half sibs' family size, these accuracies were 0.87 and 0.84 in family size of 20 and 4, respectively. As half sibs family size is high, accuracy of breeding appeared high. Based on the results of this study it is concluded that the amount of marker information, heritability and family size would influence the accuracy of the estimated breeding values in genomic selection methodology for animal breeding.

• Korean Journal of Ecology and Environment
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• v.42 no.3
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• pp.290-294
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• 2009

Larval development of Nannopaya pygmaea Rambur (Libellulidae), an endangered dragonfly in Korea, was studied using an introduced lariat population in an artificial wetland habitat. Artificial habitat was created in a green house which imitated a small wetland in Boryeong-si, Chungcheongnam-do, Korea, where N. pygmaea inhabited. A total of 300 N. pygmaea larvae were introduced to the artificial habitat in June 2007. Larvae were recaptured five times between June 2007 and November 2008 for measurement of body length. As a result, the initial and recaptured populations (Recaptured I, II, III, IV, and V populations, respectively) contained two body size groups [initial 6.20$\pm$0.34 mm and 7.94$\pm$0.46 mm (mean$\pm$SD); Recaptured I 2.84$\pm$0.43 mm and 5.16$\pm$0.83 mm; Recaptured II 5.96$\pm$0.66 mm and 8.02$\pm$0.35 mm; Recaptured III 5.97$\pm$0.73 mm and 7.82$\pm$0.37 mm; Recaptured IV 7.04$\pm$0.93 mm and 8.52$\pm$0.39 mm; Recaptured V 5.72$\pm$0.60 mm and 7.71$\pm$0.30 mm]. Our rearing experiment evidenced that the recaptured I-V populations are the offspring of the initial population and the offspring grew approximately 3 mm at 470 degree days. It was also estimated that N. pygmaea larvae need approximately 100 degree days to grow 0.7 mm in body length.

• Journal of Animal Science and Technology
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• v.55 no.3
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• pp.165-171
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• 2013

Genomic relationship matrix (GRM) was constructed using whole genome SNP markers of swine and genomic breeding value was estimated by substitution of the numerator relationship matrix (NRM) based on pedigree information to GRM. Genotypes of 40,706 SNP markers from 448 pure Landrace pigs were used in this study and five kinds of GRM construction methods, G05, GMF, GOF, $GOF^*$ and GN, were compared with each other and with NRM. Coefficients of GOF considering each of observed allele frequencies showed the lowest deviation with coefficients of NRM and as coefficients of GMF considering the average minor allele frequency showed huge deviation from coefficients of NRM, movement of mean was expected by methods of allele frequency consideration. All GRM construction methods, except for $GOF^*$, showed normally distributed Mendelian sampling. As the result of breeding value (BV) estimation for days to 90 kg (D90KG) and average back-fat thickness (ABF) using NRM and GRM, correlation between BV of NRM and GRM was the highest by GOF and as genetic variance was overestimated by $GOF^*$, it was confirmed that scale of GRM is closely related with estimation of genetic variance. With the same amount of phenotype information, accuracy of BV based on genomic information was higher than BV based on pedigree information and these symptoms were more obvious for ABF then D90KG. Genetic evaluation of animal using relationship matrix by genomic information could be useful when there is lack of phenotype or relationship and prediction of BV for young animals without phenotype.

• Korean journal of applied entomology
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• v.54 no.4
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• pp.383-392
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• 2015

We investigated the induction of pupal diapause and number of generation for H. armigera using outdoor rearing and sex pheromone trapping in Suwon, Korea. Over-wintering pupae were induced when neonate larvae were reared in the outdoors from late Aug. to early Oct. in 2013 and 2014. H. armigera adults emerged from late May to early Jun. for 2013 colonies and from late May to late Jun. for 2014 colonies. The colonies placed after mid September produced only diapause pupae, to show environmental conditions that day-lengths on the rearing start date were 11 h 49 min~12 h 24 min, and mean temperatures before pupation were $14.8{\sim}20.7^{\circ}C$. Summer diapause was not observed in all colonies. The peak occurrence of H. armigera adults from sex pheromone trap in Suwon and Hwaseong were pooled and showed four generations (1st: from late Apr. to mid Jun., 2nd: from mid Jun. to late Jul., 3rd: from mid Jul. to late Aug., 4th: from late Aug. to mid Oct.). A degree-day model for development of H. armigera developed by Mironidis and Savopoulou-Soultani (2008) was used to validate the number of generation from field observations using pheromone traps. The 3rd and over-wintering generations were mainly overlapped. It was decided that H. armigera has one over-wintering and three complete generations in a year, and diapause is induced from offsprings of the 3rd and 4th generations adults. It is expected that larvae of the 1st and 2nd generations give a damage to ear zone in maize fields in which have been planted during April.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.34 no.3
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• pp.18-27
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• 2016

A 'flowered-wall,' which are also called a 'flower plant patterned wall,' or a 'flower patterned wall,' is a wall of a building or a fence with various patterns on it. A flowered-wall can be an external mean to look into the owner's authority and values while internally it possesses the symbolic meaning of wishing the well-being and peace of the household. In the research area of the flowered-wall, the walls located in the palace were well researched in various studies across architecture, horticulture, and art design, however, the walls belonging to the local regional traditional folk houses and temples have been involved in little to no research in the landscape architectural area. Taking notice of this perspective this study researched and analyzed the cases of the flowered-walls of the traditional folk houses and the temples that belonged to the national and municipal cultural properties of Jeollabuk-do Province from the landscape architectural perspective. The whole samples were examined and it was shown that there were 9 traditional folk houses with flowered-walls. Among the temples there were 7 cases. Therefore the research focusing on tracing the shape and symbolic meaning of the flowered-walls in the 16 cultural properties located in the Jeollabuk-do Province which consisted of traditional folk houses and temples resulted in the following. Flowered-walls displayed hierarchical differentiation revolving around the main space and its spacial characteristic. This differentiation is variously displayed across the flowered-wall, gable, crack plastering, and chimney. In the case of the folk houses the symbols have the meaning of the prosperity of the household and progeny, exorcism, longevity, number of fortune, harmony, and peace etc that prays for practical wishes such as long life and good health with the prosperity of their descendants. Meanwhile in the temples, symbols indicating an easy passage into eternity, perpetuation of the Buddha-nature, and three marks of existence are applied, differentiating from the folk houses by the appliance of the religious values in the patterns. In conclusion this research resulted in the rightful illumination on the local landscape culture, the possibility of expressing the Korean sentiment through flowered-walls in the contemporary space, the reassessment of flowered-walls, and the provision of basic data for a plan to success the cultural heritage.