• Journal of Embryo Transfer
• /
• v.30 no.3
• /
• pp.207-211
• /
• 2015

Many pronuclear stage eggs were used to generate transgenic mice (Tg) by microinjection. In this study, we used in vitro fertilized mouse eggs, followed by ultrarapid freezing to establish a simple procedure for production of Tg mice. We produced in vitro fertilized mouse eggs and cryopreserved them by ultrarapid freezing method. A total of 139 cryopreserved-thawed pronuclear eggs, of which 101 (72.6%) were survived following microinjection of chicken ${\beta}-actin$ promoter-driven firefly improved luciferase cDNA (${\beta}-act/luc^+$) and were transferred into 5 recipients. All recipients became pregnant and gave birth to a total of 15 (14.8%) pups. As a control, same DNA construction (${\beta}-act/luc^+$) was also injected into 450 in vitro fertilized eggs, of which 338 (75.1%) were survived and then were transferred into 14 recipients. Eleven (78%) mice became pregnant and littered a total of 54 (19.1%) pups. Southern blotting analysis of Tg mice indicated that one (1/15, 6.6%) and three (3/54, 5.5%) transgenic mice were production from cryopreserved and in vitro fertilized eggs, respectively. All Tg mice produced from both eggs showed the expression of improved luciferase gene. These results indicated that efficiency of produced of Tg mice from cryopreserved eggs was comparable to that from in vitro fertilized eggs. Furthermore, it is suggested that microinjection of transgene into in vitro fertilized eggs cryopreserved by ultrarapid freezing is an easy and conveniently method for production of Tg mice.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.13 no.6
• /
• pp.1387-1396
• /
• 2018

The purpose of this study was examined the effect of Tabata exercise using health care application on balance, gait and activities of daily living in patients with stroke. Twenty patients with stroke were participated patients with stroke in this study. Nineteen participants were randomly allocated to ten in experimental and nine in control groups. The both groups received the rehabilitation therapy during for 4 weeks. The experimental group performed Tabata exercise based on health care application, supervised under caregivers, during 30 min/day. The control group performed self-exercise supervised under caregivers, during 30 min/day. Both groups exercised for 4 weeks, 3 times a week, 30 min/day. Tabata exercise was trained by an physical and occupational therapists. The outcome measures were the BBS(: Berg Balance Scale), TUG(: Timed Up and Go, K-MBI(: Korean Modified Barthel Index). In results, the experimental groups showed a significant improvements after intervention in all assessments(p<.05), The control groups showed a significant improvements after intervention in BBS(p<.05) The experimental group showed a greater significant improvements than control group in TUG, BBS, K-MBI(p<.05). We suggest that Tabata exercise using health care application can have a effects of the improvements of balance, gait and activities of daily living than only self-exercise in patients with stroke.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.41 no.2
• /
• pp.77-83
• /
• 2021

Rye (Secale cereal L.) is the most tolerant to abiotic stress including low temperature, drought, and unfavorable soil conditions among the winter cereals. Rye is the rapid growth of early spring results from increasing areas for the use of the forage and green manure in the middle part of Korea. "Jungmo2509", a rye cultivar was developed by the National Institute of Crop Science (NICS), RDA in 2014. It was developed from a cross between "Olhomil", a self-compatible cultivar, and "Synthetic II", a self-incompatible line. "Jungmo2509" is an erect plant type and of a middle size, with a green leaf color, a yellowish-white colored culm, and a yellowish brown-colored, small-size grain. The heading date of "Jungmo2509" was April 23, which was 5 days later than that of "Gogu", respectively. But "Jungmo2509" showed greater resistance to lodging compared to that of the check cultivar, with similar to winter hardiness, wet injury, and disease resistance. "Jungmo2509" was a higher to than "Gogu" in terms of protein content (9.4% and 8.0%, respectively), total digestible nutrients (TDN) (55.7% and 55%, respectively). The seed productivity of "Jungmo2509" was approximately 2.08 ton 10a-1, which was 11% lower than that of the check. Almost all rye cultivars are out-crossing due to genes controlling incompatibility, but "Jungmo2509" is higher seed fertility (56%) than that of Gogu (0%). it has self-compatible genes. "Jungmo2509" is erect in plant type and resistance for lodging. Therefore, "Jungmo2509" can produce uniform seeds for processed grains of human consumption and utilize them as parents for breeding the rye hybrids with high forage yields.

• Journal of Korea Water Resources Association
• /
• v.54 no.7
• /
• pp.475-484
• /
• 2021

The optimal design for water distribution system (WDS) is not only satisfying the minimum required water pressure of the nodes, but also minimizing pipe cost, etc. The number of designs of WDS increases exponentially due to the arrangement of various pipes. Various optimization algorithms were applied to propose an optimized design of WDS. In this study, Modified Hybrid Vision Correction Algorithm (MHVCA) with improved self-adapting parameter was applied to optimal design of WDS. The performance was improved by changing the Hybrid Rate (HR) of the existing Hybrid Vision Correction Algorithm (HVCA) to nonlinear HR. To verify the performance of the proposed MHVCA, it applied to mathematical problems consisting of 2 and 30 decision variables and constrained mathematical problems. In order to review the application results of MHVCA, it was compared with Harmony Search (HS), Improved Harmony Search (IHS), Vision Correction Algorithm (VCA) and HVCA. Finally, MHVCA was applied to the optimal design problem of WDS and the results were compared with other algorithms. MHVCA showed better results than other algorithms in mathematical problems and WDS problem. MHVCA will be able to show good results by applying to various water resource engineering problems as well as problems applied in this study.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.41 no.5
• /
• pp.608-618
• /
• 1996

Wild perennial types of genus Glycine, as sources of genetic diversity, have been recently employed in the soybean breeding programs. This study was carried out to introduce some useful characters of wild perennial Glycine tomentella into common cultivars, G. max. Parent materials used for interspecific hybridization were three common cultivars, Ulsankong, Baemkong and Danyeobkong, and four G. tomentella strains having different diploid(2n) of 38, 40, 78, and 80. Their cross-fertility, effect of reciprocal cross, development comparison of crossing pod and optimum timing to efficient crossing were investigated. Plant were grown in 1/2,000 Wagner pots in greenhouse and field. Crossing efficiency and pod survival rate were the highest in 2n=38 of the four G. tomentella strains when the common cultivars were fixed as female parents. In the reciprocal cross of three common cultivars and four G. tomentella strains, when the former was used as maternal sources, they showed better crossing rate and longer pod survival than the latter. In the interspecific hybridization between G. max cv. Baemkong andG. tomentella, 2n=38, the pod formed from the female G. tomentella had relatively greater size and weight than that from the female Baemkong in comparison with parents. However, the reverse result was true in a number of ovules per pod. More efficient interspecific hybridization in the field was made on August 20, when temperature and relative humidity were higher and day length was shorter, than one or two months earlier.

• Journal of the Korean GEO-environmental Society
• /
• v.24 no.12
• /
• pp.47-52
• /
• 2023

In order to efficiently maintain heat pipes operated by district heating operators, the facility history and damage history data built by the operator are used to identify key independent variables that are related to the occurrence of damage. Afterwards, the correlation with the frequency of damage was analyzed, and a basic model for estimating the frequency of damage was derived. Considering the correlation with the estimation model based on the use time currently being used by domestic and foreign district heating operators, a simple regression analysis basic model was presented as the independent variable with the highest correlation between continuous variables such as the use time, pipe diameter, burial depth, and insulation level of monitoring system, and the frequency of damage. The remaining independent variables were reflected as factors that modify and supplement the basic model. As a result of the analysis, as in previous research cases, it was confirmed that the analysis model between use time and frequency of damage had the highest correlation between the two variables and could be used as a basic model. Pipe diameter, burial depth, and insulation level of monitoring system information have also been confirmed to have a correlation with the frequency of damage, so they can be used as factors to supplement the basic model.

• Korean Journal of Social Welfare
• /
• v.59 no.2
• /
• pp.65-88
• /
• 2007

This study evaluates psychometric properties of the Revised Memory and Behavioral Problems Checklist(RMBPC) in Korean version. The sample includes 387 South Korean family caregivers who provide the majority of day to day tasks and emotional care for their older family members with physical and/or cognitive disabilities. The psychometric properties of the RMBPC are verified by item analysis, exploratory factor analysis(EFA) & confirmatory factor analysis(CFA). For cross-validity, the randomly divided two samples are used: one sample for EFA(n=193, 387/2) and the other sample for CFA(n=194, 387/2). The internal consistency of the K-RMBPC is excellent, and the convergent and criterion-related validity of the K-RMBPC with related variables is empirically confirmed. An EFA based on the Principal Component Analysis(PCA) method with varimax rotation explains 65.85% of variance accounted for by the three factors. A CFA also verifies that the model fit was acceptable. The shorter Korean version of the RMBPC is found reliable and valid. The translated checklist would be very useful for clinical and research settings by (a) focusing on observable, conceptually relevant, potentially modifiable behaviors and (b) using objective criteria within a self-administered framework, to enable clinicians and researchers to pinpoint areas of disturbance and target intervention goals for patients and caregivers in a cost-effective manner.

• Korean Journal of Construction Engineering and Management
• /
• v.14 no.3
• /
• pp.3-11
• /
• 2013

The changes in construction business have impact on overall operation of construction companies. Poor business of construction companies following a s low industrial cycle could have broader implications and influences on the industry. Since a construction project involves various stakeholders including public organizations, financial institutions and households, a downturn in construction industry might lead to significant economic loss. In this regard, it is meaningful to examine the relationship between changes in construction business cycles and insolvency of construction companies. This study conducts an empirical analysis of the relationship between construction business cycles and how much they affect operation of construction companies. To this end, KMV model was used to estimate probability of bankruptcy, which represents business condition of a construction company. To examine construction business cycles, investment amount for different construction types-residential, non-residential, and construction work-was used as a variable. Based on the investment amount, VECM was applied and the analysis results suggested that construction companies should put priority on diversifying project portfolio. In addition, it was shown that once a construction company becomes unstable in business operation, it is hard to recover even when the market condition turns for the better. This suggests that, to improve business operation of a construction company, internal capacity-building is as important as the market condition and other external circumstances.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.11
• /
• pp.137-147
• /
• 2018

This study is a descriptive evaluation of the differences in biomedical ethics consciousness, related knowledge, awareness, and attitude of nursing college students according to the completion of biomedical ethics education. The data were collected from 112 nursing students using self-report questionnaires and analyzed by t-test, Chi-Square test, Fisher's exact probability test and Pearson's correlation coefficient using SPSS 21.0. There were significant differences between the two groups in the sub-domains of biomedical ethics consciousness, artificial insemination, euthanasia and organ transplantation. The biomedical ethics consciousness was positively correlated with attitudes toward human tissue donation and transplantation, awareness of death, and awareness of hospice palliative care. The results of this study showed that after participating in the biomedical ethics education, nursing college students had statistically significant differences in the consciousness of artificial insemination, euthanasia, and organ transplantation. Moreover, the consciousness of biomedical ethics was related to attitude toward human tissue donation and transplantation and awareness of hospice palliative care. Based on the results of this study, we would like to suggest the need for development of various curricula related to death, hospice palliative care, human tissue donation and transplantation in the biomedical ethics curriculum. In addition, as the study was conducted in a single college, it is difficult to generalize the results; therefore, additional studies at different facilities are recommended.

• The Journal of Korean Academy of Prosthodontics
• /
• v.61 no.3
• /
• pp.227-233
• /
• 2023

Tardive dyskinesia is an involuntary neurological movement disorder caused by long-term use of dopamine receptor-blocking drugs leading to dental implications like uncontrolled gnashing and grinding of teeth which in turn imperil the oral rehabilitation procedures as the excessive load increases the risk of prosthesis fracture. A 40-year male with a medical history of tardive dyskinesia visited the hospital to receive oral rehabilitation for missing maxillary anterior teeth. After the oral examination, tooth preparation was done on teeth 13, 15, and 23. After that silicon impression was made and the gypsum cast was digitalized using a desktop scanner and an interim prosthesis was fabricated by milling a resin block. During the try-in, the occlusal one-third of the interim prosthesis was trimmed, and an auto-polymerizing acrylic resin was applied on the occlusal surfaces and inserted in the patient's mouth. Then, the functionally generated path (FGP) of occluding surfaces of opposing arches was traced on the resin surface. When the resin was hardened, the modified interim prosthesis was removed and digitized using an intraoral scanner. The scan image was used in designing the occlusal morphology of definitive prosthesis by modifying the design of the interim prosthesis using the dual scan method. Lastly, a monolithic zirconia prosthesis was fabricated by milling a zirconia block. The definitive prosthesis was delivered reflecting the patient's occlusal scheme. This case report shows that the FGP technique with the dual scan method can help in fabricating fixed prosthesis with harmonious occlusion in a tardive dyskinesia patient.