• Title/Summary/Keyword: 자가수정

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자가 인공수정기의 국제적 실증 결과

  • 이명식;최창용;오운용;이지웅;장원경;박수봉;백광수;우제석
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.3-3
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    • 2001
  • 전문가의 영역인 인공수정기술을 초보자도 용이하게 수정이 가능하도록 자궁경관 외도구의 영상을 체외에서 육안관찰하면서 수정적기 포착 및 적정부위내 정액 주입 화인이 가능한 축산기술연구소 개발 자가인공수정기의 국내외적으로 실증시험을 통하여 수태율을 알아보고자 수행하였다. 국내에서 직장질법으로 전업수정사가 수정하였을 때 72%(54/75)로써 자가인공수정기로 해당축주가 수행한 결과 67.5%(50/74)와 비교하여 1회 수정수태율이 다소 떨어지는 경향이었으며 품종별 수태율은 한우 61.7%(21/31), 젖소 60.0%(15/25)로 차이가 없었고 4개 지역의 145두에 자가인공수정한 결과 90두가 임신되어 62.0%의 수태율을 얻었다. 중국에서 중국황우를 대상으로 150두를 수정한 결과 1회 수정수태율이 84.6%(127/150)였고, 재차 수정 결과 98%(147/150)로 높은 수태성적을 얻었다.

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Selection of Self-Fertilization Stevia and Development of Multiple Propagation Method using Hypocotyl Explants (자가수정 스테비아의 선발과 배축절편체를 이용한 대량증식 방법 개발)

  • Seo, Seung Min;Bae, Shin-Chul;Kim, Kyung-Min
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.442-447
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    • 2011
  • This study was carried out to assess the potential of hypocotyl explants for multiple-propagation by intensive sweetener variety with high yielding in stevia of self-fertilization. Agronomical character of self-fertilization shown a height range 72.0~120.7 cm, branch's 4.9~56.6 in stevia. Selection of high content of Rebaudioside A (RA) from segregating population in mixing variety have a variety, 'DA1' (55.2%) from HPLC analysis. On a developing of multiple-propagation in stevia, hypocotyl explants used to select for reasonable medium under the influence of each plant growth regulators concentrations. In a Mix treatment of plant growth regulators, as Murashige and Skoog medium with 1.5 mg/L BA and 0.5 mg/L Kinetin showed the most favorable. We got the multiple-propagation of 30 shoots from one inoculated hypocotyl explants as the result of these bases. Rooting induced superbly on MS medium with 0.1 mg/L IBA with inoculated shoots. All of the rooting plants were adapted to a normal seedling. This method of multi-propagation is used effectively in Rebaudioside A productivity elevation.

Growth, Flowering, and Ripening Seed Characteristics of Mammillaria goldii, M. theresae, and M. pseudopectinata (선인장 Mammillaria goldii, M. theresae와 M. pseudopectinata의 생육, 개화 및 종자형성 특성)

  • Song, Cheon Young;Lee, Dong Chul;Park, In Tae;Park, Wha Soon;Hong, Seung Min
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.285-290
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    • 2009
  • This experiment was conducted to investigate the propagation system by seed to check growth, flowering, and making seed of three-year-old cactus of potted Mammillaria goldii. M. theresae, and M. pseudopeclinata. Plant height and width of them was 2.5~2.8 cm, and 2.8~3.1 cm, and seed formation of Mammillaria goldii and M. theresae had internal body taking130 to 135 days to ripening seeds, however, M. pseudopectinata had externals taking just 26 days to ripening seeds. The mean seeded pod from April to June of M. goldii was 50.0% and the number of seeds in a pod was 25.3. In M. theresae, the mean seeded pod from April to June was 57.3% and the number of seeds in a pod was 35.0. However, the highest rate of seeded pod on May of M. pseudopectinata was 75.0% and the number of seed in a pod was 66.0. The sib crossing was much more ripening seed fertilization than that of self cross ing in the Mammillaria. The self crossing was formed from 12.0% to 20.0% of seeded pod, from 12.0 to 16.0 number of seed in a pod. However, sib crossing obtained 58.0% of seeded pod, 30 seeds in a pod of M. goldii, 65.0% seeded pods and 40 seeds in a pod of M. theresae, and 75.0% seeded pod, 68 seeds in a pod of M. pseudopectinala as the highest of them.