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PEROXIDASE ACTIVITY ASSOCIATED WITH Puccinia sorghi INFECTION IN MAIZE (옥수수에 있어서 녹병감염에 관한 Peroxidase의 활성)

  • Kim Soon Kwon;Brewbaker James L.;Hasegawa Yoichi
    • Korean journal of applied entomology
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    • v.17 no.4 s.37
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    • pp.193-199
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    • 1978
  • Five parents and their 10 $F_1$ crosses of maize (Zea mays L.) were tested by means of diallel analysis for the inheritance of peroxidase activity following Puccinia sorghi rust infection. Peroxidase activity was measured at day zero and at 2 days and 8 days after inoculation. Peroxidase activity was increased significantly by P. sorghi infection in all 15 genotypes after 8 days, but not after 2 days. Highly significant differences in peroxidase activity were detected among the 15 genotypes. The highly genera] resistant inbred, CM105, and its hybrids showed exceptionally high peroxidase activity in both healthy and infected plants. However, another highly resistant inbred, Oh545, showed exceptionally low peroxidase activity. Significant general combining ability (GCA) and specific combining ability (SCA) means quares were detected for peroxidase activity independent of disease. GCA mean spuares, however, were consistently a major contiribution to the inheritance of peroxidase activity in the infected plants whereas SCA men square contributions were minor. Rust resistant maize plants controlling mongenic dominant $Rp_1^d$gene showed stronger peroxidase reroxidase responses than their susceptible counterparts in the gel electrophoresis and densiometric tracings. The increased peroxidase activity occurred in both major leaf peroxidases, $Px_3\;and\; Px_7$.

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Calculation of the Correction Factors related to the Diameter and Density of the Concrete Core Samples using a Monte Carlo Simulation (몬테카를로 전산해석을 이용한 콘크리트 코어시료의 직경과 밀도에 따른 보정인자 계산)

  • Lee, Kyu-Young;Kang, Bo Sun
    • Journal of the Korean Society of Radiology
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    • v.14 no.5
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    • pp.503-510
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    • 2020
  • Concrete is one of the most widely used materials as the shielding structures of a nuclear facilities. It is also the most generated radioactive waste in quantity while dismantling facilities. Since the concrete captures neutrons and generates various radionuclides, radiation measurement and analysis of the sample was fulfilled prior to dismantle facilities. An HPGe detector is used in general for the radiation measurement, and effective correction factors such as geometrical correction factor, self-absorption correction, and absolute detector efficiency have to be applied to the measured data to decide exact radioactivity of the sample. Correction factors are obtained by measuring data using a standard source with the same geometry and chemical states as the sample under the same measurement conditions. However, it is very difficult to prepare standard concrete sources because concrete is limited in pretreatment due to various constituent materials and high density. In addition, the concrete sample obtained by core drill is a volumetric source, which requires geometric correction for sample diameter and self absorption correction for sample density. Therefore in recent years, many researchers are working on the calculation of effective correction factors using Monte carlo simulation instead of measuring them using a standard source. In this study we calculated, using Geant4, one of the Monte carlo codes, the correction factors for the various diameter and density of the concrete core sample at the gamma ray energy emitted from the nuclides 152Eu and 60Co, which are the most generated in radioactive concrete.

Analysis of the Factors Influencing the Mesopore Ratio on the Soil Surface to Investigate the Site Factors in a Forest Stand(I) - With a Special Reference to Coniferous Stands - (산림(山林)의 입지환경인자(立地環境因子)가 표층토양(表層土壤)의 조공극률(粗孔極律)에 미치는 영향인자(影響因子) 분석(分析)(I) - 침엽수림(針葉樹林)을 중심(中心)으로 -)

  • Jeong, Yongho;Park, Jae Hyeon;Kim, Kyong Ha;Youn, Ho Joong
    • Journal of Korean Society of Forest Science
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    • v.90 no.3
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    • pp.314-323
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    • 2001
  • This study aimed to clarify the influencing factors of mesopore ratio on a pore geometry of surface soil in coniferous stands as an index of the water retention capacity. Twenty three factors including site conditions and soil properties were analyzed by spss/pc + for the data collected during March to October of 1993. The factors influencing the mesopore ratio(pF2.7) on the surface soil were as follows; macropore ratio(pF1.6), slope, crown-cover rates, thickness of F layer, organic matter contents, and the growing stock. And influencing factor on the ratio of mesopore in the soil surface was correlated with percentage of amount of clay, soil surface, A and B horizon soil hardness shows high negative significance. Also, multiple regression equations for mesopore ratios of surface soil and surface soil hardness, clear length, growing stock, B horizon of soil hardness, organic matter contents show high significance($R^2$; 0.80). In coniferous stands, it is effective in promoting development on the ratio of mesopore that forest practice for enhancing of the water resource retention capacity should be carried out when the crown-cover rates of stands are more than 80 percentages.

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In vivo assessment of Fibroblast growth factor(FGF)-Fibronectin fusion protein coating on titanium;Histomorphometric analysis in rabbit tibia (섬유아세포 성장인자와 파이브로넥틴 복합 단백질로 처리한 타이태늄의 생물학적 효과;가토의 경골을 이용한 조직계측학적 분석)

  • Na, Ho-Kyun;Kim, Tae-Il;Lim, Sang-Hoon;Cho, Ki-Young;Chung, Chong-Pyoung;Han, Soo-Boo;Ku, Young
    • Journal of Periodontal and Implant Science
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    • v.35 no.1
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    • pp.153-161
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    • 2005
  • 파이브로넥틴은 세포외기질에 존재하는 당단백질로 세포의 부착, 이동, 성장 및 분화에 관여하며, 섬유아세포 성장인자는 세포의 증식 이동 및 분화에 영향을 주는 중요한 성장인자로 알려져 있다. 최근 연구에 의하면, 파이브로넥틴은 조골세포의 타이태늄 임플란트 표면으로 이주와 증식 및 골생성을 촉진하며, 섬유아세포 성장 인자는 파이브로넥틴에 상승작용을 한다고 보고된 바 있다. 이 실험의 목적은 파이브로넥틴 및 섬유아세포 성장인자의 복합 단백질을 이용하여 타이태늄 임플란트의 골 반응을 알아보는 것이다. 체중 2.5 kg 내외의 건강한 18 마리의 웅성가토를 준비하여 무균 사육하였고, 순수 타이태늄을 절삭가공하여 직경 3.5mm, 길이 6mm 의 machined surface를 지니는 screw type 의 임플란트를 준비하였다. 사람의 유전자를 기초로, 유전자 재조합법을 통해, 적절한 primer를 이용하여 얻은 섬유아세포 성장인자를 파이브로넥틴 III 형 분절의 9-10 번 도메인에 결합시켜 얻은 복합 단백질을 준비된 임플란트에 표면처리하여 실험군으로 하였고, 표면처리하지 않은 임플란트를 대조군으로 하여, 가토의 좌우 경골에 각각 2 개씩의 임플란트를 식립하였다. 4주 후, 가토를 희생시켜 각 경골 당 한 개의 임플란트에서 뒤틀림 제거력을 측정하였고 나머지 임플란트 식립 부위 에서는 경골을 포함하는 조직표본을 제작하였다. 조직표본상에서 골접촉이 가장 좋은 3 개의 나사산의 길이를 측정하고, 나사와 접촉하는 골의 길이를 측정하여 골-임플란트 접촉도를 구하고, 같은 부위에서 나사산 사이의 면적과 골이 차지하는 면적을 비교하여 골생성률을 얻었다. 실험군과 대조군의 결과는 Student t-test 를 이용하여 신뢰도 95% 수준에서 통계학적 유의성을 검정하였다. 파이브로넥틴과 섬유아세포 성장인자의 복합 단백질로 표면처리된 임플란트와 표면처리를 하지 않은 임플란트는 뒤틀림 제거력에서는 통계적 유의성이 나타나지 않았으나, 골-임플란트 접촉도와 골생성률에서 복합 단백질로 처리된 임플란트가 통계적으로 유의하게 높은 결과를 보였다. 이상의 연구결과로, 섬유아세포 성장인자와 파이브로넥틴 복합 단백질로 처리한 타이태늄 임플란트가 주변 골 형성을 촉진시켜, 골유합을 증진시킴을 알 수 있었다. 따라서, 복합 단백질이 타이태늄 임플란트의 성공률을 높이기 위한 표면개질 물질로 이용될 가능성을 확인할 수 있었다.

Prediction of Regional Metastasis by the Expression of Lymphangiogenic Factors in Micropapillary Thyroid Carcinoma (미세갑상선유두상암종에서 림프관 생성인자 확인을 통한 림프절 전이의 예측)

  • Lee, Sung-Bu;Choi, Seung-Ho;Nam, Soon-Yuhl;Cho, Kyung-Ja;Kim, Sang-Yoon
    • Korean Journal of Head & Neck Oncology
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    • v.27 no.1
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    • pp.32-37
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    • 2011
  • 서 론 : 미세갑상선유두상암종 환자에서 불필요한 예방적 중앙 림프절절제술을 피하기 위해서 림프절 전이를 수술 전에 예측하는 수단이 필요하다. 림프관 생성 및 성장의 조절에 VEGF-C/D, VEGFR-3 pathway, podoplain이 관여된다는 사실이 밝혀져 있다. 림프관 생성 및 성장과 관련된 인자인 VEGF-C/D, podoplanin에 대한 면역조직화학염색과 반정량적 분석을 통해 미세갑상선유두상암종에서 림프절전이와의 관련성을 확인하고자 하였다. 대상 및 방법 : 2006년 9월부터 2008년 6월까지 본원에서 미세갑상선유두상암종으로 진단받고 1인 술자에 의해 갑상선 전 절제술 및 예방적 중앙 림프절절제술을 받은 104명의 환자 중 중앙 림프절 전이가 있었던 환자와 없었던 환자를 각각 25명씩 무작위로 선별하여 종양부위에 면역화학염색을 실시하여 림프관생성인자의 발현 정도를 비교하였다. 결 과 : 대상군 50예 중 VEGF-C/D는 50예(100%) 모두 발현이 되었고 podoplanin은 33예(66%)에서 발현이 되었다. 그 중 VEGF-C는 10예(20%)에서 약한 양성, 37예(74%)에서 중등도 양성, 3예(6%)에서 강한 양성소견을 보였고 VEGF-D는 9예(18%)에서 약한 양성, 37예(74%)에서 중등도 양성, 4예(8%)에서 강한 양성소견을 보였다. 중앙 림프절 전이 음성 환자 군과 양성 환자 군으로 분류하였을 때 VEGF-C/D의 발현율의 차이는 p-value가 각각 0.48, 1.00으로 통계적으로 유의한 차이를 보이지 않았다. 50예 전체를 대상으로 하여 종양의 개수, 최대크기, 검출된 전체 림프절의 수, 양성 림프절의 수, 주변조직 침범여부에 따른 VEGF-C/D의 발현도 통계적으로 유의한 차이를 보이지 않았다. Podoplanin의 경우 염색 여부에 따라 양성군과 음성군으로 나누어 분석하였을 때 종양의 개수, 최대크기, 검출된 림프절의 수, 양성 림프절의 수, 주변조직 침범여부도 통계적으로 유의한 차이를 보이지 않았다. 결 론 : VEGF-C/D는 대상군 전체(100%)에서 발현이 되었고 podoplanin은 66%에서 발현이 되었다. 림프관 생성인자로 알려진 VEGF-C/D및 podoplanin이 미세갑상선유두상암종에서 많이 발현이 되는 것으로 보아 위 인자들이 림프절 전이를 일으키는 인자 중 하나로 생각된다. 하지만 미세갑상선유두상암종에서 중앙 림프절 전이를 예측할 수 있는 인자로 부적합 한 것으로 생각되며 향후 더 많은 증례를 통해 관련성 여부에 대한 연구가 필요하고 또 다른 인자의 관련성에 대해서 연구가 필요하겠다.

Sensitivity Analysis of Depletion Parameters for Heat Load Evaluation of PWR Spent Fuel Storage Pool (경수로 사용후핵연료 저장조 열부하 평가를 위한 연소조건 인자 민감도 분석)

  • Kim, In-Young;Lee, Un-Chul
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.9 no.4
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    • pp.237-245
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    • 2011
  • As necessity of safety re-evaluation for spent fuel storage facility has emphasized after the Fukushima accident, accuracy improvement of heat load evaluation has become more important to acquire reliable thermal-hydraulic evaluation results. As groundwork, parametric and sensitivity analyses of various storage conditions for Kori Unit 4 spent fuel storage pool and spent fuel depletion parameters such as axial burnup effect, operation history, and specific heat are conducted using ORIGEN2 code. According to heat load evaluation and parametric sensitivity analyses, decay heat of last discharged fuel comprises maximum 80.42% of total heat load of storage facility and there is a negative correlation between effect of depletion parameters and cooling period. It is determined that specific heat is most influential parameter and operation history is secondly influential parameter. And decay heat of just discharged fuel is varied from 0.34 to 1.66 times of average value and decay heat of 1 year cooled fuel is varied from 0.55 to 1.37 times of average value in accordance with change of specific power. Namely depletion parameters can cause large variation in decay heat calculation of short-term cooled fuel. Therefore application of real operation data instead of user selection value is needed to improve evaluation accuracy. It is expected that these results could be used to improve accuracy of heat load assessment and evaluate uncertainty of calculated heat load.

Using AHP to Analyze the Evaluation Factors Related to Wildlife Passage Management (AHP기법을 활용한 야생동물이동통로의 기능개선을 위한 평가항목 분석)

  • Lee, Jin-Young;Rho, Paik-Ho;Lee, Jang-Won
    • Korean Journal of Environment and Ecology
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    • v.24 no.6
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    • pp.763-771
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    • 2010
  • The rapid increase of wildlife passage installation since the late 2000s was aimed to reduce roadkill caused by habitat fragmentation and losses related to road construction, but wildlife-vehicle collisions are now still occurred even near the wildlife passage area. This is the reason that the effectiveness of wildlife passage have not been evaluated in combination with absence of monitoring data and management strategy of the wildlife passage. The AHP method are used, in this study, to identify the evaluation factors affecting the effectiveness of the present 367 wildlife passages in a mitigation measures to reduce road effects on wildlife species. Ten evaluation factors are derived from third levels in the AHP analysis. Priority setting to identify appropriate management strategies in first level is selected among four second levels on facility, environment, wildlife species and management tool. The AHP analysis suggested that neighboring environments are the most important factor at the second level, and passage structure, harmony with natural surroundings, wildlife occurrence and monitoring of the passage are also important factors at the third levels. In summary, effective measurements of wildlife passage management is based on managing the passage with neighboring topography and natural surrounding. This is useful to establish wildlife passage management strategy in order to reduce the negative effects of roads on wildlife species.

The Study on the Limiting Factor to Determine Available Soil Depth in Korea (우리나라 토양의 유효토심 결정시 저해인자에 관한 연구)

  • Hyun, Byung-Keun;Rim, Sang-Kyu;Jung, Sug-Jae;Sonn, Yeon-Kyu;Song, Kwan-Cheol;Noh, Dae-Cheol;Lee, Heob-Seung;Hyun, Geun-Soo;Zhang, Yong-Seon;Hong, Suk-Young;Park, Chan-Won;Kim, Lee-Hyun;Chol, Eun-Young;Jang, Byeong-Chun
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.5
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    • pp.293-302
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    • 2008
  • The limiting factors to determine available soil depth were studied with 390 soil series in soil profile description and physicochemical data in Korean soils. The limiting factors were coarse sandy layer, gravel and skeletal layer, hardpan layer, cat clay layer, poorly drained layer, salt accumulated layer and bed rock layer so on. The soils of having limiting factors were 332 soil series, but soils without limiting factors were 58 soil series. Soils with limiting factors were, hardpan 5, slopeness 93, immature soil 29, cinder 5, sandy 42, gravel or skeletal 47, bedrock 19, high salt content 8, poorly drained soil 22, heavy clay 32, sulfate soil 3 and ash soil 27 etc. And the orders of available soil depth were immature > slopeness > ash > heavy clay > sandy > gravel or skeletal > hardpan > cindery > poorly drained > bedrock > acid sulfate soil > salt accumulated soil etc.

Diurnal Variations of Equilibrium Factor and Unattached fraction of Radon Progeny in Some Houses and Laboratories (가옥 및 실험실내 라돈평형인자, 비 흡착 라돈자손 비율의 일일 변동 특성)

  • Lee, Seung-Chan;Kim, Chang-Kyu;Lee, Dong-Myung;Kang, Hee-Dong
    • Journal of Radiation Protection and Research
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    • v.26 no.4
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    • pp.399-408
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    • 2001
  • The variation characteristics of radon concentration, equilibrium equivalent concentration and equilibrium factor in some houses and laboratory buildings have been studied. The variation of equilibrium factor and the unattached fraction of radon progeny with ventilation condition have been also estimated. The averages of radon concentration, equilibrium equivalent concentration and equilibrium factor were $30\;Bq\;m^{-3},\;19.6\;Bq\;m^{-3}$ and 0.65 in seven houses, while $55.0\;Bq\;m^{-3},\;31.9\;Bq\;m^{-3}$ and 0.58 in three laboratory buildings, respectively. The diurnal variation of radon concentration, equilibrium equivalent concentration and equilibrium factor in indoor showed a typical pattern that the radon concentration, equilibrium equivalent concentration and equilibrium factor increased at dawn and morning, while decreased at midday and evening. While the equilibrium factor rate deceased in the indoor environment which was well ventilated, the unattached traction of radon progeny increased. The equilibrium factor was in proportion to air pressure and humidity of indoor, whereas in Inverse proportion to temperature.

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A study on the Feasibility Analysis factor Model for Housing Development Projects (주택개발사업의 사업성분석인자 모델에 관한 연구;사업성 분석인자의 중요도 분석 및 타당성 검증을 증심으로)

  • Hong, Yeong-Geon;Kim, Young-Ai;Kim, Yong-Su
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • 2007.11a
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    • pp.249-254
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    • 2007
  • The main bodies of housing markets of today need to carry out a systematic and objective feasibility analysis even from the step of planning in order to succeed in a diverse and complex market environment. Therefore, it is meaningful to understand this housing market environment and propose a necessary model for the feasibility analysis of hosing business. In this vein, the aim of this study was to extract an actual and practical feasibility analysis factor and its importance for housing market, and then present and apply a feasibility analysis factor model to an on-site example, in order to verify the model's validity. For this, the investigator interviewed with and carried out a questionnaire survey of experts in housing development projects. Study findings are as follows:First, the feasibility analysis factor, derived in this study, could provide a ground to evaluate the feasibility of subject projects in the planning of development through an analysis index. Second, when feasibility is under the level of carrying out the projects, it is possible to reexamine the projects through extracting analysis factors of which points are under the standard and via a feedback process for improvable analysis factors. Therefore, the result of applying the feasibility analysis model of this study to actual housing development projects analytically shows that the model could provide a practical evaluation criterion to the person in charge of project development through an analysis index.

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