• Journal of Life Science
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• v.16 no.4
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• pp.676-682
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• 2006

To develop high efficiency biofertilizer solubilizing insoluble phosphates, lactate dehydrogenase (ldh) gene was isolated from Staphylococcus sp. LJ2. Genetic constructions were carried out using the pGEM-T-easy vector and pHSG398. Recombinant DNA plasmids containing the ldh gene were transferred to Klebsiella sp. DA71-1 by electroporation. The selected transformant was named as a DA71-1/pLYJ. The insoluble phosphates solubilization activity of DA71-1/pLYJ was higher than that of DA71-1 at various culture conditions. Glucose was the best carbon source for insoluble phosphates solubilization among the used carbon sources. Maximal insoluble phosphates solubilizing was found in sucrose minimal (SM) medium containing 3% glucose. The solubilizing activity of DA71-1/pLYJ against three types of insoluble phosphates, such as tri-calcium phosphate, hydroxyapatite, aluminium phosphate, were quantitatively determined. The optimal temperature and initial pH to solubilize insoluble phosphates in the SM medium was $37^{\circ}C$ and pH 5.0, respectively.

• Journal of the Korea Institute of Building Construction
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• v.19 no.4
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• pp.289-297
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• 2019

Epoxy resin adhesives are currently used as adhesives in buildings. Epoxy resin adhesives, which are organic materials, generate harmful substances when producing adhesives, and toxic substances are high in the residential space after installation. In addition, a large amount of carbon monoxide generated from organic materials in the case of a building fire leads to personal injury. This study evaluates the feasibility of inorganic adhesives using pure inorganic materials such as magnesia, phosphate, and borax as inorganic adhesives to replace existing organic adhesives. As a result of the experiment on the selection of adequate phosphate and the characteristics of the addition rate of borax used as a retarder, the potassium phosphate monobasic was obtained as a suitable phosphate and the characteristics according to the borax addition rate were compared with the quality standard of KS F 4923 The hardening shrinkage and heat change rate satisfied the quality standards. The tensile strength was satisfactory when the borax addition rate was 4% or more, but the adhesive strength did not meet the quality standards. Further studies are needed to improve adhesion strength.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.4
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• pp.475-488
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• 1995

In order to see the seasonal variation of nutrients and the limiting factors to the primary production in Deukryang Bay, both dissolved inorganic nutrients and salinity were measured in the surface waters during the periods from July 1992 to March 1993. The mean value of salinity was the lowest in ?all and the highest in early spring. Dissolved inorganic nitrogen (DIN) was the highest in winter and the lowest in summer. However, both phosphate and silicate were the highest in summer and the lowest in fall. Salinity was generally higher in the outer region than in the inner region of the bay.DIN content was nearly depleted (less than $2{\mu}M$) in summer. From fall to spring, DIN content was nearly depleted in the inner region and relatively high in the outer region of the Day. Phosphate was the highest in summer showing an opposite distribution pattern to salinity, and it was nearly depleted (less than $0.1{\mu}M$) in fall and winter. In spring, however, phosphate content was slightly high in the outer region. Silicate content showed an opposite distribution pattern to salinity in summer. in other seasons, However, the distribution pattern of silicate was similar to the salinity. DIN seemed to be a limiting factor for the primary production at all area of the bay in summer and at the inner region in other season. However, phosphate seemed to be a limiting factor at all area of the bay in fall and winter and at the inner region in spring. Silicate may limit the production of diatoms at the inner region of the bay in winter and spying. Both phosphate and silicate showed a good inverse relationship with salinity in summer, which indicates inputs of these nutrients from the freshwater runoff. In the other seasons, both nitrate and silicate showed a positive linear relationship with salinity in the outer region of the bay, suggesting that these two nutrients were mainly supplied by the inflow of the offshore costal water which had high nitrate content associated with vertical mixing.

• Proceedings of the Korean Society of Computer Information Conference
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• 2023.01a
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• pp.165-166
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• 2023

물환경정보시스템에서 하천의 수질을 측정한 자료를 대상으로 DMS(Digital Mosquito Monitoring System)가 설치된 위치와 수질 측정지 위치가 가까운 4개 지점에 대한 수질 측정 성분과 모기지수의 연관성을 분석하였다. 수질 기준 외 측정 성분과 모기지수의 상관관계는 총질소(T-N), 용존총질소(TDN), 인산염인이 연관성이 높은 것으로 나타났다. 이 중에서 인산염인은 모기지수와 양적 선형관계를 이루는데, 인산염은 수중에 부영양화를 일으키는 성분 중 하나다. 수질 측정지는 비료의 영향보다는 오수의 유입으로 인산염이 과잉공급되는 것으로 보였다. 따라서 매년 바뀌는 모기지수 산출식의 지연일자 데이터에 부영양화 지수를 넣음으로써 모기지수의 정확도를 보완할 수 있을 것으로 판단된다.

• Journal of the Korean Society of Marine Environment & Safety
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• v.20 no.6
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• pp.627-639
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• 2014

In respect of the nutrients cycling in coastal environment, regeneration in bottom layer is one of major source of nutrients. We analyzed the bottom water quality at the 14 stations during 9 years from 2004 to 2012 to investigate the characteristics of nutrients at bottom layer in Jinhae Bay. Concentrations of DIN, DIP and DSi showed the large seasonal variation and were higher in summer. Especially, average concentrations of these nutrients were two times higher in hypoxic season than in normoxic season. In summer, high concentrations of DIN, DIP and DSi caused by regeneration were common feature, but spatial distribution of DSi differ from that of DIN and DIP. DIN and DIP were higher in Masan Bay, while DSi was higher in Masan Bay as well as in center of Jinhae Bay. In comparison with DIN and DIP, DSi was significantly affected by nutrients regeneration at bottom layer in whole season. According to time series analysis, DIN concentration was decreased from approximately $14{\mu}M$ to $6{\mu}M$. This result induce that Si:N ratio at bottom layer in Jinhae Bay changed from approximately 1 to 3.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2014.11a
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• pp.41-42
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• 2014

현대 산업에서 철은 매우 중요한 재료이다. 다양한 산업적 요구에 맞게 제철산업에서도 다양한 성질의 강을 개발하고 생산하고 있다. 강의 주요 물성으로는 인장강도, 항복강도, 연신율 등이 있으며 이러한 물성들을 향상시키거나 변경하는데 다양한 첨가원소와 제조 공법들이 적용되어진다. 본 연구에서는 그 중 합금 원소를 첨가하여 강도를 높이는 초고강 강에 대해서 화성처리 특성을 연구하고 그에 영향을 미치는 인자에 대해서 분석하였다. 그리고 화성처리 공정을 개발하여 초고강도강의 인산염특성을 향상 시켰다.

• Applied Biological Chemistry
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• v.24 no.2
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• pp.105-111
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• 1981

Growth responses of Brevibadterium ammoniagenes BA 17-2, which had been obtained by the treatment of several mutagens in our previous report, were investigated to select the preliminary optimal concentrations of phosphate, $Mg^{++}$, $Mn^{++}$ and thiamine for the production of 5'-XMP aminase. In this experiment it was shown that the concentration of phosphate in the medium has an important effect on the growth of microorganism. Using the medium containing 0.2% of $MgSO_4{\cdot}7H_2O$, 3mg/l of $MnSO_4{\cdot}H_2O$and $1\;mg/l$ of thiamine-HCl, the maximum cell mass was obtained at the concentration of 0.4% of $KH_2PO_4$ and $K_2HPO_4$, respectively. Above the concentration of these phosphates, cell growth was inhibited as the phosphate concentration increased to 1%, but the inhibition was overcome by the addition of 1% of $MgSO_4{\cdot}7H_2O$ and 3mg/l of thiamine-HCl. The 5'-XMP aminase activity was also influenced by the concentration of phosphate, $Mg^{++}$, $Mn^{++}$, and thiamine. In addition, the optimal culture pH and temperature for the enzyme activity were found to be 6.8 and $32^{\circ}C$, respectively.

• Journal of Applied Biological Chemistry
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• v.57 no.2
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• pp.171-174
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• 2014

$\small{L}$-Arginine was applied to processed pork sausages and evaluated as a substitute for commonly used inorganic polyphosphate. Processed pork sausage was prepared with an addition of 0.25, 0.5, and 1.0% (w/w) $\small{L}$-arginine. Quality characteristics of the processed pork sausages were than evaluated in terms of pH, moisture content, emulsion stability and textural properties. The pH of sausage increased with increasing $\small{L}$-arginine concentration. Moisture content of 0.25% $\small{L}$-arginine sausage, emulsion stability of 0.5% $\small{L}$-arginine sausage, and textural properties of 1.0% $\small{L}$-arginine sausage were similar to those of 0.5% polyphosphate sausage, respectively. In terms of sensory evaluation, taste and overall acceptability of 0.5% $\small{L}$-arginine sausage were slightly higher than those of 0.5% polyphosphate sausage and textural properties were reversed. According to sensory score and quality characteristics, $\small{L}$-arginine had a potential as a substitute for inorganic polyphosphate and the optimum concentration of $\small{L}$-arginine was around 0.5%.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.5
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• pp.394-400
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• 1990

In the previous paper, we reported that the bacteriostatic effect of condensed phosphate. The present study was intended to observe influence of various environmental factors on the bacteriostatic effect of condensed phosphates in the laboratory media, in order to get the information on the possibility to use the phosphate as food preservative. Bacteriostatic effect of sodium polyphosphate was not reduced by the heating at 100 for 1 hour, but it was considerably decreased by heating at $121^{\circ}C$ for 15 min and the phosphate sensitivity of bacteria was increased by freezing and heating. On the other hand, the strong bacteriostatic activity of condensed phosphate was observed below pH 6.5 in nutrient broth culture, and the activity was decreased by the addition of $CaCl_2$, KCl and $MgSO_4$.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.164-164
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• 2000

일반적으로 해양에서 전선이란 서로 다른 수괴간의 불연속면을 일컫는다. 이러한 전선의 양측 면에는 유속, 수온, 염분, 수질 등이 급변한다. 본 연구는 1997년 8월 26일부터 9월 2일까지 제주도 서부에서 중국 상해 양쯔강 하구역까지 21개 정점에서 관측된 물리ㆍ화학적 관측자료로부터 연안과 외양간의 수온, 염분, 밀도 등 물리적 인자 특성으로부터 전선역을 찾아내고 전선역을 중심으로 양측의 수질특성을 용존산소, 인산염, 질산염, 규산염 등 영양염류의 분포 특성과 아울러 생산력 인자인 엽록소 a의 분포량을 파악하여 전선역을 중심으로 한 수괴간의 특성을 규명하였다