• Microbiology and Biotechnology Letters
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• v.37 no.3
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• pp.197-203
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• 2009

An indole oxygenase originated from Rhodococcus sp. RHA1 was cloned into the expression vector, pTrc99A, in Escherichia coli, and designated pTCAN1. The pTCAN2 was constructed from pTCAN1 by the deletion of $lacI^q$ for the constitutive expression of indole oxygenase without adding IPTG in the medium. The complete open reading frame of indole oxygenase was 1,224 bp long, which encodes a protein of 407amino acids. Crude extracts of E. coli $DH5{\alpha}$/pTCAN1 and pTCAN2, respectively, were prepared and subjected to SDS-PAGE analysis. A band corresponding to molecular mass of about 43 kDa was appeared and this result correlated with the predicted molecular mass of cloned indole oxygenase. The E. coli harboring pTCAN1 and pTCAN2, respectively, showed blue color colony in LB plate. The pigment showing blue color was prepared from E. coli $DH5{\alpha}$/pTCAN2, and identified as indigo by experiments using spectrophotometer, HPLC, and TLC. The indigo-forming activity of indole oxygenases from the whole cell of E. coli $DH5{\alpha}/pTCAN1$ cultured at LB medium added 1mM of IPTG and that of E. coli/pTCAN2 showed about 1.75nmol/min/mg DCW (dry cell weight) and 3.85 nmol/min/mg DCW, respectively. Also, the E. coli $DH5{\alpha}$/pTCAN2 produced about $236{\mu}M$ of indigo after 48 hours incubation in TB medium supplemented with 2.5 mM of tryptophan.

• Journal of the Korean Society of Costume
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• v.45
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• pp.5-16
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• 1999

본 연구의 목적은 Navajo 직물의 역사적 발달을 고전시대를 중심으로 살펴보고 특히 외부영향에 따른 직물의 변화를 고찰하는데 있다. 구체적으로 1) 고전시대의 전통적인 직물은 어떻게 발달하였는가\ulcorner 2) 고전시대 직물의 전통적 요소와 외부영향요인은 어떤 점이 다른가\ulcorner 예 초점을 맞추었다. 연구방법은 아메리카 인디언의 이동,문화 Navajo 직조 및 직물에 대한 문헌을 바탕을 조사하였고 아리조나 주립박물과 아리조나 역사 박물관을 방문하였으며 박물관 안에 있는 전문가들의 조언을 듣고 연구의 자료를 보완하였다. 또 비교 미학적 차원에서 고전시대 navajo 직물의 전통적 요소와 외부영향 요인을 분석하였고 외부영향을 받은 직물의 예를 시각적자료로 제시하였다. 이연구는 인디언직물에 대한 이해, 고전시대. Navajo 직물의 문헌고찰을 통한 민족의복의 문화적측면에 기여할수 이고 현대적 감각을 지닌 Navajo 직물을 한국직물에 접목 응용하는데 도움을 줄수 있다 본 연구의 결과는 다음과 같다. 1. Navajo 직물을 고전, 전환, 양탄자 시대로 구분되었고. 이중에서 고전시대가 navajo 직물의 특성을 가장 잘 나타내었으며 발달된 직조기술을 보였다. Navajo인들의 직조기술은 1863년경 최고조에 달했다. Navajo 직물의 디자인 주제로 주로 인간을 둘러싸고 있는 환경에서 얻어졌고 이것들은 직선의 기하학적 형태로 구체화되었다. 또한 직물에 표현된 색도 자연과 밀접한 관련성을 갖는 있었다. 2. Navajo 직물의 주용한 용도는 여성용 드레스, 남성용 셔츠는 어깨에 걸치는 두르개였다. 여성용 드레스는 동일한 크기의 직물의 두장을 직조한 후에 꿰매었고 남성용 셔츠는 머리가 들어갈수 있는 구멍이 있는 장방형 판쵸 스타일이었다. 어깨에 걸치는 두르개는 여성의 경우 가운데가 검은색이고 가장자리가 푸른색인형태오 흰색과 붉은 색이 교대로 나타나는 형태의 2가지가 있었고 남성용은 고전시대의 대표라고 일컬어지는 족장 두르개였다. 3. Navajo 직물은 외부의 영향을 받아 많은 변화를 가져왔다. 즉 스페인 사람들로 인하여 면 섬유대신 양모섬유를 사용하게 되었고 전통적 모양과 다른직물이 출현하게 되었다. 또한 인디고 염류, 색소니 실, 바에타 직물의 도입으로 다양한 색상의 표현이 가능해졌고 이와 관련하여 다이아몬드 십자형 톱니형 무늬드이 나타나게 되었다. 4, 고전시대를 대표하는 직물로 족장 두르개, 쇼올, 안장덮개를 들수 있으며 이 직물들에서 뚜렷하게 외부영향 요인을볼수 있다. 즉 족장 두르개의 가장 정교한 단계에서 다이아몬드 무늬가 가장가리 가운데 모서리에 위치하여 9지점 배치를 이룬 것 쇼올의 경우 폭보다 길이가 긴 형태의 비전통적 모습을 나타낸 것 안장덮개에서 보여지는 여덟포인트 별 무늬도 외부의 영향을 받은예이다. 뛰어난 직조기술로 유명한 navajo인들은 변화에 잘 적응하는 특성을 갖고 있었다. 외부의 영향을 그들은 긍정적으로 받아들였고 자기 자신들의 필요에 맞도록 수정하여 정체감을 잃지 않으면서도 문화를 발전시켰다. 따라서 고전시대의 Navajo 직물은 고유적 요인과 외래적 요인의 조화를잘 나타내고 있으며 디자인의 탁월함이 세련됨 천연염료와 인조염료의 배\ulcorner에 의한 색상의 우월성 등으로 오늘까지 높이 평가되고있다.

• Journal of Digital Convergence
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• v.18 no.5
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• pp.65-74
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• 2020

The objective of this study is to analyze educational web-sites and digital contents for elementary music class according to the 2015 revised music curriculum. First, the current status of educational web-sites that are most actively used in music class were reviewed. And the characteristics of the digital contents included in the web-sites for music education were analyzed. the primary analysis was about the service of the web-sites, and the secondary analysis was about the type of digital contents and the contents of music education. The most actively used web-sites in music classes were Edunet T-Clear, T-Sherpa, i-scream, Indischool, which differed slightly from the web-site's services and the type of digital content and contents. Specifically, the main functions for supporting music classes, the reflection of the curriculum contents, the systematicity of the materials, the objectivity and the on-site nature, and the connection with other subjects appeared differently. In order to effectively utilize digital contents in elementary music class, first, expertise of teachers for timely use of web-sitesand digital contents is required. Second, the development and utilization of various digital contents for the nature of music education is required.

• Journal of Environmental Impact Assessment
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• v.22 no.6
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• pp.591-607
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• 2013

The objectives of the study were to evaluate the aquatic environment of an urban stream using various ecological parameters of biological biomarkers, physical habitat quality and chemical water quality and to develop a "Multimetric Eco-Model" ($M_m$-E Model) for the ecosystem evaluations. For the applications of the $M_m$-E model, three zones including the control zone ($C_Z$) of headwaters, transition zone ($T_Z$) of mid-stream and the impacted zone ($I_Z$) of downstream were designated and analyzed the seasonal variations of the model values. The biomarkers of DNA, based on the comet assay approach of single-cell gel electrophoresis (SCGE), were analyzed using the blood samples of Zacco platypus as a target species, and the parameters were used tail moment, tail DNA(%) and tail length (${\mu}m$) in the bioassay. The damages of DNA were evident in the impacted zone, but not in the control zone. The condition factor ($C_F$) as key indicators of the population evaluation indicator was analyzed along with the weight-length relation and individual abnormality. The four metrics of Qualitative Habitat Evaluation Index (QHEI) were added for the evaluations of physical habitat. In addition, the parameters of chemical water quality were used as eutrophic indicators of nitrogen (N) and phosphorus (P), chemical oxygen demand (COD) and conductivity. Overall, our results suggested that attributes of biomarkers and bioindicators in the impacted zone ($I_Z$) had sensitive response largely to the chemical stress (eutrophic indicators) and also partially to physical habitat quality, compared to the those in the control zone.

• Korean journal of food and cookery science
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• v.6 no.4 s.13
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• pp.33-39
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• 1990

Rice and waxy rice starches were adjusted to 27% of moisture and heated at $100^{\circ}C$ for 16hours, respectively. After the treatment, their gelatinization properties were investigated. The initial gelatinization temperature, obtained by transmittance and amylogram, of Akkibare and Taebaek starches were $60{\sim}65^{\circ}C$ but those of waxy rice and the U.S.A. rice starches were $55{\sim}60^{\circ}C$ and $70{\sim}75^{\circ}C$, respectively. The gelatinization temperatures of heat-moisture treated starches were higher than those of the untreated starches. Viscosities at each temperature and the highest viscosity reduced by some degrees after the treatment. DSC thermograms of all starches showed single endotherm and the gelatinization enthalpies were $2.26{\sim}2.63\;cal/g$. The gelatinization enthalpy tended to decrease after the treatment. Transmittance and viscosity by alkali showed in this order; Akkibare and Taebaek starches>waxy starch>the U.S.A. rice starch. The heat-moisture treated starches increased viscosities in every starch. Transmittance in alkali solution of Akkibare, Taebaek and waxy rice starches decreased. but that of the U.S.A. rice search increased.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.46 no.5
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• pp.368-375
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• 2018

In the present study, aerodynamic load analysis for a floating off-shore wind turbine was conducted to examine the effect of periodic platform motion in the direction of 6-DOF on rotor aerodynamic performance. Blade-element momentum method(BEM) was used for a numerical simulation, the unsteady airload effects due to the flow separation and the shed wake were considered by adopting a dynamic stall model based on the indicial response method. Rotor induced downwash was estimated using the momentum theory, coupled with empirical corrections for the turbulent wake states. The periodic platform motions including the translational motion in the heave, sway and surge directions and the rotational motion in the roll, pitch and yaw directions were considered, and each platform motion was applied as a sinusoidal function. For the numerical simulation, NREL 5MW reference wind turbine was used as the target wind turbine. The results showed that among the translation modes, the surge motion has the largest influence on changing the rotor airloads, while the effect of pitch motion is predominant for the rotations.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.3
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• pp.209-214
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• 2012

Indigo crop, Persicaria tinctoria H. Gross is an annual plant containing natural colorant, the blue dye indigo, and local cultivars had been cultivated to produce natural indigo for textile dyeing in Korea since ancient times. Naju No. 2 is a new mass-selected line from the mother population, Naju Local cultivar. In this study, two breeding lines of Naju Local and Naju No. 2, have been cultivated in four different locations, the South regions of Korea, to compare plant growth and yield characteristics between two lines. Naju No. 2 was higher in plant height, and Naju Local has more 1st branches. Naju No. 2 has larger leaf area and higher width/length ratio of leaf, showing the round leaf type as morphological stable character without regional differences. Though there was considerable regional variation in fresh and dry top weight of harvested plant, the significant difference of plant weight between two lines were not shown. The ratio of leaf to total shoot of dry weight of Naju No. 2 was higher than one of Naju Local, indicating that Naju No. 2 has better yielding of colorant which is synthesized mostly in leaf. Naju No. 2 contained more Niram (crude indigo extract) and indigo, and showed much blueness at dyeing of silk using fresh leaves than Naju Local. We concluded that a new line, Naju No. 2 could be a superior cultivar due to having higher leaf yield and better quality of natural colorant than Naju local cultivar.

• Korean Journal of Environmental Agriculture
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• v.20 no.2
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• pp.93-98
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• 2001

A chilling-tolerant japonica-type and a chilling-susceptible indica-type of rice (Oryza sativa L.) plants were compared with respect to various physiological parameters during chilling and subsequent recovery. The japonica-type and the indica-type of rice cultivars used were Ilpumbyeo and Taebaekbyeo, respectively. The two rice cultivars exhibited little or no differences in the changes of leaf fresh weight and chlorophyll content during chilling at $5^{\circ}C$ for 3 days. During subsequent recovery at $25^{\circ}C$, however, Ilpumbyeo restored its growth more rapidly than Taebaekbyeo. Since the changes of relative water content, malondialdehyde production, an estimate of lipid peroxidation, and chlorophyll fluorescence were significantly different in the two rice cultivars during the chilling and subsequent recovery, they were found to be more sensitive physiological parameters than fresh weight and chlorophyll content. However, the differences in relation to water content, malondialdehyde production, and chlorophyll fluorescence between the two rice cultivars were smaller during chilling than those during subsequent recovery. These results suggest that relative water content, malondialdehyde production, and chlorophyll fluorescence could widely be used as important physiological parameters for screening chilling-tolerant plants.

• Journal of Sensor Science and Technology
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• v.10 no.2
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• pp.101-107
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• 2001

A highly selective, sensitive and reliable ozone sensing $In_2O_3$ thin film was fabricated by a sol-gel method. The fabricated film is operated at a relatively lower temperature than ever developed thin films and saved operating power. $In_2O_3$ films deposited by sol-gel technique has been recently attracted because it is an economical and energy saving method and precisely controlled microstructure. Indium alkoxide precursor was synthesized from the reaction between indium hydroxide and butanol. PVA binder was used to improve adhesion of the films. The $In_2O_3$ thin films were obtained by spin coating from 1 to 5 times followed by drying at $100^{\circ}C$ and calcining at $600^{\circ}C$ for 1h. The film thickness was controlled by the number of coating time. The morphology and the thickness of the $In_2O_3$ films were examined by a SEM and XRD. The $In_2O_3$ thin films show a high sensitive to ozone gas at operating temperature of $250^{\circ}C$. The $In_2O_3$ sensor has very good selectivity to $CH_4$, CO, $C_4H_{10}$ and ethanol.

• Applied Chemistry for Engineering
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• v.18 no.5
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• pp.516-521
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• 2007

The copolymerization characteristics of a newly-synthesized catalyst, $rac-Me_2Si(2-p-tolylindenyl)_2ZrCl_2$, and its analogue, $rac-Me_2Si(Ind)_2ZrCl_2$, were examined in the ethylene/1-octene copolymerization while varying the concentration of 1-octene in the reaction mixture. The activity of $rac-Me_2Si(2-p-tolylindenyl)_2ZrCl_2$ catalyst was decreased with increase of comonomer concentration, which is different from the usual comonomer effect of the metallocene catalysts with a bridge structure. The contents of 1-octene in the copolymer from the catalyst with 2-p-tolyl substituent were higher than those from the catalyst without that substituent. The melting point, crystallinity, and molecular weight decreased with comonomer content which was more apparent for $rac-Me_2Si(2-p-tolylindenyl)_2ZrCl_2$ catalyst.