• Journal of Korean Society of Forest Science
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• v.71 no.1
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• pp.66-73
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• 1985

Factors affecting isolation and fusion of protoplasts of three Quercus species were investigated and procedures for isolation, purification and fusion of protoplasts of the three species were also established. Unhardened leaves and rapidly growing callus cultures were good source of viable protoplasts. The optimum composition of enzyme mixture for rapid isolation of protoplasts from leaf mesophyll tissues and calli was Cellulase Onozuka R-10 (20g/l, Macerozyme R-10(10g/l), Pectinase(250 units/l, $CaCl_2$, $2H_2O$(14mM), $MgSO_4{\cdot}7H_2O$(1.8mM), $KNO_3$(1.0mM), $H_3BO_3$(1.0mM), $KH_2PO_4$(0.2mM), KI($1.0{\mu}M$), 1,4-dithiothreitol (0.1mM) and mannitol (0.6M). Optimum density of protoplasts for maximum fusion was $2{\times}10^5/ml$ which was the highest protoplast density given in this study. Optimum concentration and duration of PEG 1450 treatment for inducing fusion appeared to be 29%(W/V) final PEG 1450 concentration and 5-10 minutes, respectively.

• Korean journal of applied entomology
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• v.27 no.3
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• pp.149-158
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• 1988

Nematophagous fungi were successfully isolated by baited plating, centrifugation technique of soil, and direct isolation from naturally ingested nematodes. Predominant seven fungi isolated were identified as Artheobotrys arthroboteyides, A.conoides, A. oligospora, Dactylella lobata, Fusatium oxysporum, Monacrosporium ellopsoporum and Harposporium anguillu-lae. Of these, six fungi were tested for cultural characteristics except. H, anguillulae, extre-mely fastidious fungus in artificial media. Among 14 media tested in this experiment, Corn-meal Agar (CMA) and Oatmeal Agar (OMA) were the most suitable media for growing all six nematophagous fungi. Weakly saprophytic M. ellipsospoyum also grew vigoroualy on these two media. The radial growth, dry weight and sporulation of the fungi tested were quite diverse depending on the culture media. D. lobata revealed good growth and abundantly sporulated on Glucoes Peptone Agar (GPA). Although over-all growth of F, oxysporum was not satisfactory on Sucrose Nitrate Agar (SNA), the sporulation was best on this medium. Optimum conditious for mycelial growth and sporulation of nematophagous fungi ranged pH 5-8 and 20-$30^{\circ}C$ on SNA. D. lobata and F, oxysporum grew vigorously and most profusely sporulated on all media tested. They turned out an most promising biocontrol agents for their aggressive growth and sporulation over the ranges of temperature and pH ranges.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.152-155
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• 2012

A new Saxifraga fortunei 'Pinkle' was bred by crossing 'Juno' with 'Hongoan', both with light pink (RP-N66A)-colored petals. The selection of promising lines and characterization of flowers were conducted from 2004 to 2008 year in greenhouses of the Highland Agriculture Research Center, NICS, RDA. The flowering of 'Pinkle' starts on October 7 and lasted for 27 days. The number of flowers per plant is 99.7 with many flowering habits. The flower show mixed dark pink (R-P N66C) colors at opening. The flower have 5.0 petals, each have the size is 2.9 cm in width and 3.2 cm in length. Mean plant height is 15.8 cm and number of leaves are 44.3 ea. 'Pinkle' can be used as a pot flower. Fifty percent shading of the sunlight is recommended and soft rot disease occurs caused by over-irrigation. 'Pinkle' was registered as a new cultivar in Korea Seed and Variety Service in April 2011.

• Journal of Life Science
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• v.24 no.6
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• pp.626-632
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• 2014

The genetic structure and phylogenetic relationship were investigated in Korean red spotted grouper populations using the nucleotide sequence polymorphisms of the mitochondrial DNA (mtDNA) cytochrome c oxidase subunit I (COI) gene. The COI gene was sequenced showed 99.1-99.8% identity with the EF607565 sequence previously reported. A total of twenty haplotypes were found, and the Korean population showed nineteen haplotypes. Among those, Hap_03 and Hap_08 showed Jeju-do and China-specific COI sequences, respectively. However, Hap_07 had twelve COI sequences from South Korea and records from Hong Kong and Taiwan. Neighbor-joining (NJ) trees constructed from the phylogenetic analyses based on the polymorphisms of the COI haplotypes showed a monophyletic branching pattern within the genus Epinephelus. This indicated that the red spotted grouper populations had evolved from common maternal ancestors. In addition, the Hap_08, which had the COI sequence recorded only from China Sea, was found in the middle of the NJ tree nearby Hap_07 and showed a close relationship with Hap_07. This indicates that Chinese red spotted grouper is also maternally related to other populations in East Asia. Consequently, East Asian red spotted grouper populations are maternally related, as well as sharing the same evolutionary history, and are still affected by the East Asian ocean current (Kuroshio). These findings help to explain the genetic structure and phylogenetic relationship of red spotted grouper and also contribute to research on artificial breeding and industrialization.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.8 no.4
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• pp.861-866
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• 2007

This study was conducted to investigate an aeration tank with RBC process attached Bacillus sp. known as a suitable microorganism for the removing of organic carbon, nitrogen and phosphorus. An aeration tank was based on tapered aeration because Bacillus sp. was well grown in this like environment conditions. The biofilm process with Bacillus sp. as an advanced treatment process could be a best technology for the prominent removal of organic carbon, nitrogen and phosphorus if the mechanism in the process is verified. The operation conditions of DO in the tapered aeration tank were maintained as $1.2{\sim}1.5mg/L$ in aeration tank1, as $0.3{\sim}0.5mg/L$ in aeration tank 2 and less than 0.2 mg/L in aeration tank 3, respectively. Lab-scale experiments were conducted, at room temperature, internal recycle rate was from 200% to 50% and returned sludge rate was from 100% to 50%. As a result, concentration of organic carbons, nitrogen and phosphorus in Period 1 (the time of Bacillus sp. adapted to environment) were decreased gradually. Ultimately, each removal rate in this biological experiment were TCODCr 94%, BOD 87%, T-N 85%, T-P 89% in Period 2. Hence, this process showed an excellent performance of the removal of organic carbon, nitrogen and phosphorus and this is an effective system fur treating of wastewater.

• Journal of Chest Surgery
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• v.40 no.1 s.270
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• pp.1-7
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• 2007

Backgound: It has been shown that the endothelium of cardiac valves and adjacent great vessels have a reduced immune reaction compared to other vessels. We investigated the clinical feasibility of using immunologically untreated xenogenic valves, in a pig-to-goat pulmonary valve conduit implantation model. Material and Method: Porcine pulmonary valve conduits were prepared without specific immunologic treatment and implanted into the right ventricular outflow tract of goats while undergoing cardiopulmonary bypass. Two goats each were assigned to the following observation time intervals: one day, one week, three months, six months and twelve months. Echo-cardiographic examinations were performed prior to sacrifice of the goat to evaluate pulmonary valve function. After the xenograft specimens were retrieved, histological changes were evaluated microscopically. Result: Ten of the twelve animals survived the predetermined observation time intervals. Aneurysmal dilatations, of the anterior wall of the implanted pulmonary artery, were observed at each of three and twelve month-survival animals. A variable degree of pulmonary valve regurgitation was observed on echocardiography. However, valve stenosis, thrombotic occlusion and vegetation were not seen. Microscopically, the nuclei of the donor tissue disappeared as a result of pyknosis and karyolysis; however the three components of the implanted xenografts (the pulmonary artery, the valve and the infundibulum) were gradually replaced by host cells over time, while maintaining their structural integrity. Conclusion: Immunologically untreated xenogenic pulmonary valve conduits were replaced by host cells with few observed clinical problems in a pig to goat pulmonary valve implantation model. Therefore, they might be an alternative bioprosthesis option.

• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.245-250
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• 1998

Tobacco mosaic virus(TMV) coat protein cDNA was transformed to Nicotiana tabacum cv. NC82 and the transgenic tobacco plants resistant to TMV infection were isolated in the next generation. The expression of TMV coat protein cDNA and genetic stability of the fifth generation of TMV resistant transgenic tobacco plants at the higher temperature were investigated. The TMV coat protein cDNA was amplified by genomic PCR in all the TMV resistant transgenic tobacco plants. The TMV coat protein expressed in the transgenic tobacco plants was detected at very low level by immunoblot hybridization. Even in tansgenic plants that showed the viral symptom only on very late sucker growth (delay type plants), the coat protein expression in the suckers was much less than that of susceptible tobacco infected with TMV. The TMV coat protein expressed in the transgenic tobacco plants was below 0.01% of total protein. Transcription and expression of the coat protein cDNA in delay type plants were observbed at high temperature (38$^{\circ}C$), and TMV replication was suppressed at both 28$^{\circ}C$ and 38$^{\circ}C$. This indicates that unlike the resistance conferred by 'N' gene. TMV resistance of transgenic tobacco plant won't break down at high temperature.

• The Korean Journal of Food And Nutrition
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• v.16 no.1
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• pp.29-34
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• 2003

Prunus mume has been used for the folk medicine by many old civilizations to treat food-borne diseases or enteric disorders. This study was carried out to examine the antimicrobial effect of juice from Prunus mume against pathogens such as Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. The juice of Prunus mume had the strongest antimicrobial activity to Sal. enteritidis. The concentrations of Prunus mume juice for the formation of clear zone were 1% for Sai. enteritidis(15.0mm), 3% for Lis. monocytogenes(14.7mm), and 5% for Bac. cereus(14.75mm), Esc. coli(13.45mm) and Sta. aureux(11.9mm). The growth of all tested microorganisms was inhibited apparently in tryptic soy broth containing 3% and 4% Prunus mume juice. And it was found that the Prunus mume juice showed the highest antimicrobial properties, followed by Sal. enteritidis, Bac. cereus, Sta. aureus, Lis. monocytogenes, Esc. coli.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.595-598
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• 2009

A new yellow gerbera (Gerbera hybrida Hort.) cultivar "Golden Time" was developed from a cross between '99-ga-2' and '99-na-7-6', followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) in 2007. Detailed characteristics of the new cultivar were evaluated from 2004 to 2007. "Golden Time" has semi-double type large flowers in a good harmony with yellow (RHS, 17-B) ray floret and brown center. It has good, stable flower shape and strong peduncles, and its vase life was 12.3 days. The average flower yield of "Golden Time" was about 49.5 stems per plant/year in greenhouse trails during 2004 and 2007. This cultivar was registered to the Korea Seed and Variety Service (KSVS) for commercialization in 2009. Year-round production of this cultivar is possible in the greenhouse condition throughout the Korean climate.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.216-219
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• 2010

An Asiatic lily cultivar 'Yeri' was developed in 2005 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Korea. The cross was made in 1993 between Asiatic lily 'Geneve', a light pink colored cultivar, and 'Montreux', deep purple colored cultivar. The first selection was done and was tentatively named as 'A95-68' in 1995. After in vitro multiplication and bulbing production, growth and flowering characteristic tests were conducted from 1996 to 2003. The evaluation of characteristics was performed and named as 'Wongyo C1-19' in 2005 that was registered as 'Yeri' to the registration office of Korea Seed & Variety Service. 'Yeri' flowered at the first of July and grew average 34.6 cm stem in length. Flowers bloomed facing upward, unspotted in petals and deep purple (RHS, RP58A). The size of flower was 13.3 cm. Mean petal length and width was 7.7 cm and 2.7 cm, respectively. Leaves were 5.1 cm long and 0.5 cm wide, respectively. The weight and size of bulb were 9.6 g and 11.7 cm, respectively. Year-round flowering can be done by storing the bulb under $-1.5^{\circ}C$ conditions. For forced cultivation, it was necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. It was needed to control Botrytis disease in wet season.