• Clinical and Experimental Reproductive Medicine
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• 제36권2호
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• pp.137-142
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• 2009

난소과자극증후군은 보조생식술에서 배란유도 및 과배란유도 시 발생할 수 있는 가장 심각한 합병증으로 알려져 있다. 흉수는 중증 난소과자극증후군에서 10%에서 보고되고 있고, 주로 심한 복수와 동반되어 나타난다. 하지만, 심한 복수를 동반하지 않는 중증 편측 흉수는 드물게 보고 있고, 아직 병태생리에 대해 명확히 밝혀진바 없다. 본 저자들은 과배란유도 및 인공수정과 관련된 오태아 임신에 의해 유발된 경미한 복수와 중증 편측 흉수를 동반한 난소과자극증후군을 경험하였고, 흉수천자와 같은 침습적 시술 없이 보존적 치료만으로 호전되었다. 이 증례를 경험하여 간단한 문헌고찰과 함께 보고하는 바이다.

• 한국임상수의학회지
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• 제31권1호
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• pp.51-53
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• 2014

생후 10일령 수컷 진도견이 무뇨증을 주 증상으로 하여 내원하였다. 신체검사를 통하여 포피 및 포피구멍이 존재하지 않아 선천적인 비뇨생식기 기형으로 진단하였다. 무뇨증을 치료하기 위하여 요도 탐색술을 실시하였다. 정중하복부의 피하에서 음경을 확인하고 음경을 외부로 노출시킨 후 피부를 봉합하였다. 환축이 성장함에 따라 음경이 주변 피부조직에 교약 및 건조되며 울혈이 발생하였다. 이에 대한 수술적인 치료로써 파하직에 터널을 만들어 인공적으로 포피와 포피구멍을 만드는 재건술을 실시하고, 이 부위로 음경을 환납하였다. 이후 합병증 없이 정상적인 배뇨를 보였다. 포피의 무형성이 있으나 음경이 정상적인 위치에 존재한다면 포피 및 포피구멍 재건술을 통하여 정상적인 배뇨를 할 수 있을 것으로 사료된다.

• 한국수산과학회지
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• 제38권3호
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• pp.164-171
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• 2005

Codium fragile (Suringar) Hariot, an edible green alga is farmed in Korea by natural blooming zygotes attachment. Experiments were conducted to reveal the conditions for artificial seed production of C. fragile by sexual and asexual reproduction. Growth was compared between zygotes attachment (sexual reproduction) and isolated utricles with medullary filaments (asexual reproduction). Zygotes and isolated utricles with medullary filaments were cultured under different light conditions (10, 20, 40, 60 and $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and temperatures (5, 10, 15, 20 and $25^{\circ}C$) under 16:8LD. Maximum growth of zygote was $261.3{\pm}21.0\;{\mu}m$ under $15^{\circ}C$ and $20\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ after 13 days culture. Maximum regeneration of isolated medullary filament was $8.1{\pm}1.7\;mm$ per one isolated utricle under $20^{\circ}C$ and $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ after 15 days culture. After intermediate culture during two months in the field, morphogenesis occurred in both sexual and asexual reproduction, and growth of young thalli was not significantly different (p>0.05) between the both reproduction methods. Even though seed production of C. fragile is possible in both sexual and asexual reproduction, the mass artificial seed production of asexual reproduction is much more effective than that of sexual reproduction that is too much affected by maturity.

• Clinical and Experimental Reproductive Medicine
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• 제25권2호
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• pp.217-231
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• 1998

The effectiveness of intrauterine insemination (IUI) combined with controlled ovanan hyperstimulation (COH) in the treatment of infertility with various etiologies was compared in a total of 152 cycles. Patients received a maximum of three IUI cycles for the treatment. Severe male ($<2\times10^6$ motile sperm) or age factor (> 39 y) patients were excluded in this study. Pregnancy was classified as clinical if a gestational sac was seen on ultrasound. The overall clinical pregnancy rate was 7.9% per cycle (12/152) and 9.7% per patient (12/124). The pregnancy rates were 0% in unstimulated natural (0/18), 7.5% in CC (3/40), 8.2% in CC+hMG (4/49), 5.9% in GnRH-a ultrashort (1/17), 5.9% in GnRH-a long (1/17) and 27.3% in dual suppression cycles (3/11), respectively. The pregnancy rate was higher in dual suppression cycle than other stimulated cycles, but this was not significant. The multiple pregnancy rates were 25.0% (2 twins and 1 triplet). No patient developed ovarian hyperstimulation. Abortion rates were 66.7% in CC (2/3) and 100% in ultrashort cycles (1/1). The livebirth rate was 5.9% per cycle (9/152) and 7.3% per patient (9/124). There were no differences in age, duration of infertility, follicle size, total ampules of gonadotropins and days of stimulation between pregnant and non-pregnant groups. However, significant(P<0.05) differences were observed in the level of estradiol $(E_2)$ on the day of hCG injection ($3,266.6{\pm}214.2$ vs $2,202.7{\pm}139.4$ pg/ml) and total motile sperm count ($212.1{\pm}63.4$ vs $105.1{\pm}9.9\times10^6$) between pregnant group and non-pregnant group. These results suggest that IUI combined with successful ovarian stimulation tends to improve the chance of pregnancy as compared to IUI without COH and a total motile sperm count may be considered predictive of the success for pregnancy.

• Clinical and Experimental Reproductive Medicine
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• 제26권1호
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• pp.31-41
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• 1999

To evaluate the effectiveness of intrauterine insemination (IUI) in the treatment of infertility, timed-intercourse and intrauterine insemination by husband in stimulated cycles with clomiphene citrate and gonadotropins were compared in a total of 105 cycles. Patients received 100mg of clomiphene citrate daily for 5 days starting on day 3 of the menstrual cycle followed by hMG or FSH. Doses of exogenous gonadotropins were adjusted by the follicular development and concentrations of serum estradiol $(E_2)$. More than 3 follicles reaching >16 mm were present in the ovary, 5,000 IU of hCG was administered intramusculary. Patients received a maximum of three intercourse or IUI cycles for the treatment. Severe male (<$10{\times}10^6$ motile sperm) or age factor (>39 y) patients were excluded in this study. Pregnancy was classified as clinical if a gestational sac or fetal cardiac activity was seen on ultrasound. The overall clinical pregnancy rates were 17.1% per cycle (18/105) and 21.2% per patient (18/85). The pregnancy rates (per cycle) were 17.5% (11/63) in intercourse and 16.7% (7/42) in IUI groups, respectively. IUI had no significant improvement in pregnancy rate compared with timed-intercourse. The multiple pregnancy rates were 11.1% (1 twin and 1 triplet). No patient developed ovarian hyperstimulation. Abortion rate was 28.6% (2/7) in IUI group only. The delivery and ongoing pregnancy rates were 15.2% per cycle (16/105) and 18.8% per patient (16/85). There were no differences in age, duration of infertility, follicle size and level of estradiol $(E_2)$ on the day of hCG injection in pregnant and non-pregnant groups. However, total doses of gonadotropins were higher in pregnant group than in non-pregnant group (p<0.01). Pregnancy rate was not affected by ovulatory status at the time of insemination. These results indicate that well timed-intercourse in stimulated cycles is as effective as IUI for infertile couples.

• Clinical and Experimental Reproductive Medicine
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• 제26권3호
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• pp.433-440
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• 1999

Objective: To evaluate the effectiveness of CC+FSH or CC+hMG in intrauterine insemination (IUI) cycles for the treatment of infertility. Method: Patients received daily 100 mg of clomiphene citrate (CC) for 5 days followed by hMG or FSH. A single IUI was performed at 36 h after hCG. Clinical pregnancy was classified if a gestational sac or fetal cardiac activity was seen on ultrasound. Results: The overall clinical pregnancy rate was 19.1% per cycle (17/89) and 21.5% per patient (17/79). More clinical pregnancies were recorded in CC+FSH (23.1%, 6/26) than CC+ hMG cycles (17.5%, 11/63), but this difference was not statistically significant. No differences were found in age, duration of infertility, follicle size, levels of estradiol ($E_2$) on the day of hCG injection and total motile sperm counts between pregnant and non-pregnant groups. However, more ampules of gonadotropins were used in pregnant group than non-pregnant group (p<0.05). Conclusion: Combination of CC and hMG may economically be more effective to induce ovulation for IUI compared to CC and FSH.

• Clinical and Experimental Reproductive Medicine
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• 제30권4호
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• pp.281-291
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• 2003

Objectives: In determining to perform non-spousal artificial insemination by donor (AID) to an infertile married couple, infertile couple requires not only the thorough understanding of the medical procedure but also scrutinizing the effect, which it will have on the relationship of the family including the baby to be born itself. Materials and methods: 148 cases with non-curable male infertility were enrolled in this inquiry survey. The donor insemination questionnaire consists of 18-items which are assessing subjects' clinical properties, the background for AID practice, psychological traits, and long term influence. Results: Of the survey, 49 cases were returned (33.1%) and 10 cases (20.4%) of these gave birth after AID practice. The mean age of husbands and wives of the 49 cases were $34.6{\pm}4.2$ and $32.1{\pm}3.0$ yers old, respectively and the duration of marriage was 5 years and 4 months. In about half of the cases, AID was first suggested by husband and the decision was made by only the couple. The major reason for the operation was to form a complete family. In the item of the psychological effects, two-third of the couples felt anxiety related to the procedure which are mostly about the possible congenital or acquired deformity of baby. The AID was positively suggested in overall by all of recipients. After giving birth to a child, most couples felt positive about their decision. As a child grows up, about half of the couples felt the child as their own and expected not to tell of the AID. In overall, about 50% of couples presented satisfaction with the procedure. Conclusions: As the above results, various psychological impacts including anxiety about a child-to-be-born were accompanied to those who were recommended of AID. To overcome these problems, sufficient medical information and consultation about the course of selecting the donor and the whole procedures of AID should be provided beforehand.

• Clinical and Experimental Reproductive Medicine
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• 제14권1호
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• pp.29-35
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• 1987

Alternative methods were used in the field of infertility as the latest means for the treatment of misconception. Artificial insemination, which has been commonly used already, IVF-ET and GIFT are also used as artificicial method for conception presently. Appling such methods to the patient, following three categories should be considered; first, there should be an understanding and an agreement from the patient, second, if possible, reduce the cost of hospital expense and relieve physical and emotional problems of the patient, and third, increcrsing the pregnancy rate. Under these considerations, complication and pitfalls of artifical inseminations are a big burden to the physian and the patient. Though the conicicental complications are relatively very rare, detailed laboratoy tests, carefull examinations, and follow-up studies are necessary. In the distribution of the age groups, 40.3% was in the 25 to 29 years group, 30 to 34 years was 45%. 35 to 39 years was 8% and only 3.2% was above the 40 age groups. The range of infertile periods were from 1 to 17 years, and half of the patients were over 5 years, the other group 20% in 3 years and 2 to 4 years were 10%. Among 159 cycles of artificial insemination, there were complications such as infection(1.9%) and discomfort(5%) and abnormal bleeding (0.6%). During pregnancy, clinical abortion (1.9%) and toxemia (2.5%) were shown. Sex of new born infants were, male (68%) and female (31%). Fortunately, there were no cases of psychological complication and genetic abnormality. Indications of artificial insemination for male factors were aspermia (2.5%), azoospermia (28.8%), oligospermia (26.4%) and asthenozoospermia (1.8%), for female factors were irregular cycle (11.7%) and dysmucorrhea.

• 현장농수산연구지
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• 제21권1호
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• pp.149-157
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• 2019

패류 인공종자생산에서 모패관리의 중요성이 커져가고 있으며 최적의 모패 관리 조건과 원하는 시기에 종자생산을 할 수 있는 성숙유도 조건을 구명하는 하는 것이 매우 중요하기 때문에 본 연구에서는 왕우럭 조개의 모패관리 최적조건과 성숙 촉진을 위한 가온 효과를 조사하였다. 모패관리는 실내사육에 비하여 실외사육이 비만도와 생존율 측면에서 더 효과적인 것으로 나타났으며 관리 측면에서도 인위적인 먹이생물 공급이 없으며 자연환경 조건에서 사육하기 때문에 관리가 수월하다는 장점이 있는 것으로 나타났다. 그리고 동계가온을 통한 성숙촉진 유도는 비만도와 생식소 관찰 등을 통하여 수온 18℃ 이상에서 2개월 정도 사육하였을 경우 성성숙이 이루어지는 것으로 나타나 성숙관리가 다른 패류에 비해 용이하다는 것을 알 수 있었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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• pp.26-26
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• 2023

포스트 코로나 이후 먹거리에 대한 소비자의 인식전환으로 건강먹거리인 야콘에 대한 관심이 증가하고 있다. 야콘은 남미 안데스에서 도입된지 40여년 되었으며 달콤한 맛과 아삭아삭한 식감을 가져 생식용과 야콘즙으로 인기있으나 현재 국내 육종된 품종이 없다. 따라서, 다양한 유전자원을 확보하고 자원에 대한 평가를 통해 신품종 육성을 위한 기초 자료로 활용하고자 실시하였다. 연구에 사용된 재료는 국립식량과학원에서 보유하고 있는 5개 자원을 대상으로 초장, 엽수, 줄기수, 생체중, 관아수량, 괴근수량 등을 조사하였으며, 조사방법은 농촌진흥청 농업과학기술 연구조사 분석기준(RDA, 2012) 준하였다. 야콘 유전자원의 지상부 특성 결과, 초장이 92-127cm 범위를 보였으며, 줄기수는 8-12개, 경엽중은 1,433-2,855kg, 엽수는 152-195개의 범위를 보였다. 또한, 유전자원별 지하부 특성을 살펴보면, 유전자 원별 HY1이 관아수가 가장 많고, 관아수량도 352/10a으로 가장 무거웠다. 괴근수량을 살펴보면, HYL2가 총수량이 5,197 kg/10a, 상품수량이 4,213 kg/10a으로로 가장 많았으며, HYL3, HYL4, HYL5, HYL1 순이었다. 유전자원의 생리장해 결과를 조사한 결과, 열근 및 부패발생은 HYL1이 가장 심하였으며, HLY5가 상대적으로 약했다. 유전자원의 품질특성을 조사한 결과, 당도는 HYL5가 12.5로 가장 높았으며, HYL1이 8.7로 가장 낮았다. 이상의 결과를 바탕으로, 향후 야콘의 인공교배 단계에서부터 육종프로그램에 활용할 계획이다.