• Title/Summary/Keyword: 이형체

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Isolation of Conditional Lethal Temperature-sensitive Mutants of Bacillus sphaericus (Bacillus sphaericus의 치사감온성 돌연변이체의 분리)

  • Kim, Young Han;Lee, Hyung Hoan
    • Microbiology and Biotechnology Letters
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    • v.13 no.1
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    • pp.41-49
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    • 1985
  • Bacillus sphaericus was mutagenized with UV light irradiation and dimethyl sulfate. Thirty-five conditional lethal temperature-sensitive(ts) mutants were isolated at the nonpermissive temperature of $42^{\circ}C$ and classified into three groups by their growth characteristics on the nutrient broth, peptone glucose yeast extract agar and mineral salts agar. First was the lethal ts group, 24 mutants, which did not grow at the nonpermissive temperature, the second, 9 mutants, was the less growth is group whose growth was restricted to one-half, and the third, 2 mutants, was the cold lethal ts group whose growth was restricted at the permissive temperature($25^{\circ}C$and $30^{\circ}C$)

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A Design of Mooring Line for the Buoy-Enabled Underwater Surveillance System (부이형 수중감시 시스템에서 계류라인의 구조 설계)

  • Byun, Yang-Hun;Choi, Bum-Kyu;Oh, Tae-Won
    • The Journal of Korean Institute of Information Technology
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    • v.16 no.12
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    • pp.41-47
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    • 2018
  • The buoy-enabled underwater surveillance system is a device that is installed in a particular sea area and operated for a certain period of tine and moved to another sea area after recovery. In this paper, a mooring method which is applied for a buoy-enabled underwater surveillance system was selected to maintain installation and enure stable operation. Also, the structure of the mooring line was designed. Two-point mooring method was selected considering interference with the communication cable of array-assembly. The composite structure of buoy chain, nylon rope, and anchor chain is designed as the basic component of mooring line. For the verification of design, a numerical simulation and wave tank experiment were performed. Their results were confirmed similarity in test condition. Finally, the mooring lines were designed for the environment of the sea trial location. The mooring line produced by the final design confirmed the stability above the significant wave height considered in the design on the sea trial.

The association between polymorphisms of ${\beta}$-adrenoceptors and preeclampsia

  • Lim, Ji-Hyae;Kim, Shin-Young;Park, So-Yeon;Yang, Jae-Hyug;Han, Jung-Yeol;Hong, Dal-Soo;Choi, June-Seek;Choi, Kyu-Hong;Ryu, Hyun-Mee
    • Journal of Genetic Medicine
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    • v.4 no.2
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    • pp.160-166
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    • 2007
  • Purpose : The ${\beta}$-adrenoceptors are pharmacologically classified into ${\beta}_1$-, ${\beta}_2$- and ${\beta}_3$-adrenoceptor. The gene of each subtype has polymorphisms related to their function (${\beta}_1$-adrenoceptor: Ser49Gly, ${\beta}_2$-adrenoceptor: Gln27Glu, ${\beta}_3$-adrenoceptor: Trp64Arg). The objectives of this study were to analyse the allelic and genotypic distribution of the representative polymorphism of ${\beta}$-adrenoceptors in preeclampsia and to investigate whether combined genotype of ${\beta}$-adrenoceptors may be associated with preeclampsia. Methods : Blood samples were collected from a Korean population (159 preeclamptic pregnancies and 168 normotensive pregnancies). The ${\beta}_1$-, ${\beta}_2$- and ${\beta}_3$-adrenoceptor genotypes was determined using polymerase chain reaction-restriction fragment length polymorphism. Results : There were no differences in allelic and genotypic distribution of ${\beta}_1$- and ${\beta}_2$-adrenoceptor polymorphisms between the two groups. However, the Arg allele of ${\beta}_3$-adrenoceptor polymorphism were more frequent in preecalmpsia than in controls (P<0.05, OR=1.57, 95% CI=1.01-2.46). Moreover, prevalence of genotype carrying heterozygote of ${\beta}_3$-adrenoceptor polymorphism was increased in preeclampsia compared with controls (P<0.05, OR 1.76, 95% CI 1.06-2.92). When combination of the three polymorphisms were evaluated, pregnancies with the particular combined genotype that is consisted of heterozygote of ${\beta}_1$-, ${\beta}_3$-adrenoceptor and wild homozygote of ${\beta}_2$-adrenoceptor (Ser/Gly, Gln/Gln, Trp/Arg), showed a significant increase in the risk of preeclampsia (P<0.05, OR=3.01, 95% CI 1.12-8.08). Conclusion : A particular combined genotype (Ser/Gly, Gln/Gln, Trp/Arg) of - adrenoceptors was associated with the risk of preeclampsia.

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Establishment of a Stable Cell Line Expressing Human BMP2/7-PTD for Efficient Osteogenic Induction (효과적인 뼈 세포분화 유도를 위한 유전자 재조합 PTD 융합 인간 뼈 형성촉진인자2/7(BMP2/7-PTD)를 발현하는 세포주 개발)

  • Park, Seung-Won;Kang, Seok-Woo;Goo, Tae-Won;Kim, Seong-Ryul;Paik, Soon-Young
    • Journal of Life Science
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    • v.22 no.4
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    • pp.456-465
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    • 2012
  • Heterodimeric recombinant human bone morphogenetic proteins (rhBMPs) are powerful tools for bone tissue engineering. However, BMPs have several important limitations in their application to bone regeneration. BMPs have a short half-life and must be used in high concentrations, which may be cost-inefficient. To overcome these problems, we established a stable cell line that expressed the fusion protein comprised of recombinant human BMP2/7 heterodimer protein and PTD (rhBMP2/7-PTD). This stable cell line enabled high process yields by continuously expressing rhBMP2/7-PTD products at high levels throughout cultivation. This synthesized BMP7 was fused to a BMP2 protein with four glycine residues (to allow free bond rotation of the domains) and PTD. To demonstrate that the rhBMP2/7-PTD protein that was secreted from an rhBMP2/7-PTD-expressing stable cell line exhibited biological activity consistent with its role as an osteogenic differentiation induction growth factor, we evaluated BMP-induced ALP activity. Our results suggest that this cell line may be a powerful and efficient tool for applications such as bone tissue regeneration.

Measurement and Analysis of Structural Grounding Effect of Concrete Pole (콘크리트 전주 구조체의 접지효과 측정 및 분식)

  • Choi, Jong-Kee;Kim, Dong-Myoung;Lee, Hyung-Soo;Shim, Keun-Bo;Kim, Kyung-Chul
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.23 no.1
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    • pp.36-40
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    • 2009
  • Concrete poles(CP) are popular supports for distribution lines. Various types of grounding electrode, such as copper-clad rods, have been used to maintain CP's ground resistance under the required value. The buried part of CP can also have structural grounding effect because of its iron reinforcing rods inside CPs. In this paper, we measured the total ground current injected into CP ground while measuring the ground current splitting to the metal electrode as well as the total injecting current. By this, it was able to measure the ground current splitting to CP structure. Based on the measured results, interrelationship between ground resistance of metal electrodes and current split factor to CP structure was analyzed.

Dual-Band Metamaterial Absorber without Metallic Back-Plate (금속 접지 판이 없는 이중대역 메타 물질 흡수체)

  • Lee, Hong-Min;Lee, Hyung-Sup
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.23 no.7
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    • pp.840-843
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    • 2012
  • In this paper, the authors present a new design for a dual-band metamaterial(MTM) absorber that utilizes resonant-magnetic inclusion of a split-ring resonator(SRR). The proposed MTM unit cell is constructed by two open complementary split-ring resonators(OCSRRs) and an SRR arrangement. To avoid the need for metallic back plate a planar array of SRRs for resonant-magnetic inclusion is placed facing toward the incident wave propagation direction. Each unit cell is printed on the two sides of a FR-4 substrate. A prototype absorber was fabricated with a planar array of $39{\times}39$ unit cells, and measured. The proposed backplane-less absorber can be used for microwave applications.

과학기술위성 3호 주탑재체 MIRIS 인증모델 개발

  • Han, Won-Yong;Lee, Dae-Hui;Nam, Uk-Won;Park, Yeong-Sik;Lee, Chang-Hui;Jeong, Ung-Seop;Mun, Bong-Gon;Park, Seong-Jun;Cha, Sang-Mok;Park, Jang-Hyeon;Lee, Deok-Haeng;Ga, Neung-Hyeon;Lee, Mi-Hyeon;Seon, Gwang-Il;Yuk, In-Su;Park, Jong-O;Lee, Seung-U;Lee, Hyeong-Mok;Matsumoto, T.
    • The Bulletin of The Korean Astronomical Society
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    • v.34 no.2
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    • pp.63.1-63.1
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    • 2009
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