This study was performed to find out stomach contents of cold water fish such as Manchurian trout (Brachymystax lenok tsinlingensis) and River salmon (Oncorhynchus masou masou) in the Odae mountain area. Field survey was carried out 3 times by each season from May to October 2012 along the Gyebang stream and the Yangyang-Namdae stream at north-west mountain slope and north-east mountain slope of the Odae mountain area respectively. The result of digestive track analysis showed that food species of Manchurian trout were total individuals of 645 including 5 orders, 23 families, 39 species of benthic macroinvertebrates and 11 taxa of terrestial insects. And each IRI (Index of Relative Importance, %) value of food species by Manchurian trout was terrestial insects 37.0%, Trichoptera 36.4%, Ephemeroptera 20.0%, Diptera 3.9%, Plecoptera 2.7%, and Gordea <0.1%. While the River salmon fed on 1,186 individuals covering 4 orders, 20 families, 33 species of benthic macroinvertebrates and 6 taxa of terrestial insects including arachnid. Also IRI values by River salmon were terrestial insects including arachnid 2.7%, Ephemeroptera 52.9%, Trichoptera 27.9%, Diptera 9.4%, and Plecoptera 7.0% respectively. With these results, we might say that both Manchurian trout and River salmon is carnivorous fish which display the feeding preference to terrestial insects and benthic macroinvertebrates. As a results we could find out that the two kinds of cold water fish preferred similar habitat types and liked same order of food species in same season, however the compositions of food species for Manchurian trout and River salmon were considerably different.
Objective : Appropriate antituberculosis chemotherapy may not prevent occurrence or progression of tracheobronchial stenosis and obstruction in the patients with endobronchial tuberculosis. The effect of corticosteroid treatment combined with antituberculosis chemotherapy was inconclusive. We evaluated prospectively the effect of corticosteroid treatment. Methods : We diagnosed endobronchial tuberculosis by bronchoscopic examination and bronchial biopsy in the patients of tuberculosis within one month of antituberculosis chemotherapy. After randomization, we prescribed isoniazid, rifampin, ethambutol, and pyrazinamide with or without prednisolone 40 mg for 4 weeks. We carried out bronchoscopy in second month and ninth month of treatment. Results : Edematous endobronchial tuberculosis showed significant improvement of bronchial stenosis after corticosteroid treatment(p < 0.05). Corticosteroid treatment did not have advantage of improvement of bronchial stenosis in the patients with infiltrative endobronchial tuberculosis. Conclusion : Corticosteroid is effective in the treatment of bronchial stenosis when endobronchial tuberculosis is edematous type, in the early period of antituberculosis chemotherapy.
The valley forests located at the east-facing slope and the west facing slope in Hambaeksan area were studied to investigate forest structure in relation to aspect and altitude of the slope. There was little difference in density. mean DBH and basal area of the tree layer between east-facing slope and west-facing slope. The importance percentages of Tilia amurensis and Betula costata in west-facing slope were higher than those in east-facing slope. However, the importance percentages of Quercus mongilica and Fraxinus rhynchophylla in the west facing slope were lower than those in east-facing slope. Species diversity of the west-facing slope was 1.415 and that of the east-facing slope was 1.328. Elevation trends were also found for forest structure. As elevation Increased basal area and mean height of the tree layer decreased in both of east-facing slope and west-facing slope. There was a tendency that number of species, species diversity and evenness decreased with increasing elevation. The importance percentage of Quercus mongolica increased with increasing elevation while those of Betula costata and Maackia amurensis decreased. The result of cluster analysis for the tree and subtree layer indicated that the studied forests were classified into the mixed forest community of broad-leaved tree species at west-facing slope and the low and middle elevation belts of east-facing slope and Quercus mongolica community at the high elevation belt of east-facing slope. Quercus mongolica was significantly and positively correlated with Symplocos chinensis for. pilosa, Acer tschonoskii var. rubripes and deutzia glabrata. Betula costata was significantly and negatively correlated with Quercus mongolica and Acer pseudo-sieboldianum.
In this experiment the effect of supplemental lighting on the growth and yield of cucumber (Cucumis sativus L. 'Fresh') plants during low radiation period of winter season were investigated in glasshouses using common high-pressure sodium (HPS) lamps and newly developed plasma lighting system (PLS) lamps. Plants grown without supplemental lighting were considered as a control. Supplemental lighting was provided from November 20th, 2015 to March 15th, 2016 to ensure 14-hour photoperiod (natural+supplemental light), also lamps were operated automatically when the outside sun radiation levels were less than $100W{\cdot}m^{-2}$. Spectral analysis showed that HPS lamp had a discrete spectrum, lacked of the radiation in the 400-550 nm wave band (blue-green light), but had a high output in the orange-red region (550-650 nm). A higher red light output resulted in an increased red to far-red (R/FR) ratio in HPS lamp. PLS had a continuous spectrum and had a peak radiation in green region (490-550 nm). HPS has 12.6% lower output in photosynthetically active radiation (PAR) but 12.6% higher output in near infra-red (NIR) spectral regions compared to PLS. Both HPS and PLS lamps emitted very low levels of ultra-violet radiation (300-400 nm). Supplemental lighting both from HPS and PLS lamps increased plant height, leaf number, internode number and dry weight of cucumber plants compared to control. Photosynthetic activity of cucumber plants grown under two supplemental lighting systems was comparable. Number of fruits per cucumber plant (fruit weight per plant) in control, PLS, and HPS plots were 21.2 (2.9 kg), 38.7 (5.5 kg), and 40.4 (5.6 kg), respectively, thereby increasing yield by 1.8-1.9 times in comparison with control. An analysis of the economic feasibility of supplemental lighting in cucumber cultivation showed that considering lamp installation and electricity costs the income from supplemental lighting increased by 37% and 62% for PLS and HPS lamps, respectively.
This study was carried out to investigate the epidermal structure and types of stomata on the upper and lower surfaces of the leaves in six species and one variety belonging to Korean Crassulaceae. The shape of epidermal cells was polygonal, isodiametric, or elongated. The cell wall was thick, sinuous or deeply sinuous. The subsidiary cell wall was thin or mostly arched. The cytolith-containing cells were found in Orostachys japonicus. The distribution of stomata was more on the upper surface than on the lower surface in the leaves of O. japonicus and Sedum sarmentosum. The stomata in the other species were less on the upper surface than on the lower surface, and stomatal size was different in each species. The great majority of stomatal types was the helico-eumesogenous type. This type was subdivided into two, parahelico-eumesogenous type and diahelico-eumesogenous type by us on the basis of the angle of division of guard mother cell and the long axis of the last-formed subsidiary cells. Sometimes allelo-eumesogenous type as well as aniso-eumesogenous type was found. The tetra-eumesogenous type with four mesogenous subsidiary cells was rarely observed. It was observed that the varieties of aniso-eumesogenous type with one to three subsidiary cells and one helix of subsidiary cells were developed by the secondary division of subsidiary cells of aniso-eumesogenous stoma. The compound shape of helico-eumesogenous stomata was found. Five new kinds of stomatal types were observed as follows; the helico-tetra-eumesogenous type, the coallelohelico-eumesogenous type, the cohelico-allelo-eumesogenous type, the duplotetra-eumesogenous type, and the aniso-euperigenous type. These types were reported here in the vascular plants for the first time.
Journal of agricultural medicine and community health
/
v.30
no.1
/
pp.39-50
/
2005
Objectives: This investigation was carried out to explore the source and the mode of transmission of the diarrhea outbreak in Seongju-gun, Gyeongbuk, 2004 Methods: The authors conducted a questionnaire survey among the 275 persons (students, staff members and cooks) who ingested the possibly contaminated foods. We also investigated the drinking water and the dining facility, and we reviewed the process of cooking the salad, which was the presumed cause of the Enteropathogenic Escherichia coli(EPEC) diarrhea. The confirmed EPEC diarrheal case was defined as culture positive for EPEC, and the suspicious case was defined as diarrheal case with symptoms more than one of fever, vomiting and tenesmus. Results: The attack rate of EPEC diarrhea was 36.7%, and there were 8 confirmed cases. The possibility of the drinking being a source of the infection was very low, for chlorine was detected in all the drinking water via reviewing the past records and using a portable detector. The foods that were significantly associated with diarrhea were found. The relative risk (RR) for the lunch served Jul 7 was 4.12 (95% CI: 1.39-12.20). Among the non-boiled foods that were finally served, the RR for the salad was 1.66 (95% CI: 1.07-2.57). The cause of this outbreak was presumed to be the contaminated foods that were prepared by cooks using rubber glove with holes, and especially the salad and foods that were served sans boiling on Jul 7. Conclusions: Though this EPEC infection was not so clinically important, if a larger outbreak occurred, it might severely affect the public health. It is recommended to develop the more safe methods for cooking foods, and to strengthen the sanitary processing foods.
Background: Accurate staging of bronchogenic carcinoma is important in determining resectability and metastasis of tumor to the subcarinal nodes is generally believed to indicate poor prognosis. The technique of Transbronchial needle aspiration (TBNA) has offered a safe & effective way to asscess mediastinal lymph node involvement in the staging of lung cancer. We performed TBNA in patients who were suspected lung cancer to evaluate the clinical usefulness of the TBNA. Method: TBNA of the subcarinal lymph node was performed at the time of initial diagnostic bronchoscopy in 60 patients with suspected lung cancer, and 42 cases of histologically proved bronchogenic cancer were analized. Results: The frequency of adequate samples by transbronchial needle aspiration (TBNA) was 81% and the positive rate of malignant cells by TBNA was 14.7%. There were no differences in positive rates by tumor cell types. In patients with thickened carina on bronchoscopy, the TBNA was positive in 33.3% as compared to 5.3% of normal carina on bronchoscopy, and the difference was statistically significant (p<0.05). In patients with enlarged subcarinal lymph node on chest CT, the positive rate of malignant cells (50.0%) was higher than that of normal sized subcarinal lymph node on chest CT (4.8%) (p<0.01). There were no specific complications in the TBNA procedure. Conclusion: TBNA is a relatively safe procedure and it offers the possibility of avoiding the cost and morbidity of surgical staging in patients especially whose carina is thickened on bronchoscopy and whose subcarinal LN was enlarged on chest CT.
Kim, Sun-Young;Suhr, Ji-Won;Shin, Kyoung-Sang;Jeong, Seong-Su;Park, Sang-Gee;Kim, Ae-Kyoung;Cho, Hai-Jeong;Kim, Ju-Ock
Tuberculosis and Respiratory Diseases
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v.43
no.2
/
pp.138-146
/
1996
Background : Known as a kind of complication or a specific form of pulmonary tuberculosis, endobronchial tuberculosis caused several kinds of problems in diagnosis and managements. But the frequency of this disease are is widely variable, generally reported from as low as 10 - 20 % to as high as 40 - 50 %. We prospectively performed bronchoscopy in patients diagnosed as pulmonary tuberculosis to evaluate the frequency of endobronchial tuberculosis and its related findings. Method : From March, 1995 to February, 1996, we prospectively performed bronchoscopy in patients newly diagnosed as pulmonary tuberculosis and evaluated the frequency of endobronchial tuberculosis, its clinical features and laboratory findings including raiologic, microbiologic and physiologic aspects. Results : Number of patients diagnosed as pulmonary tuberculosis was 103 and 55 patients(53.4%) were found to have endobronchial tuberculosis. But the frequency were 43.8% in male and 76.7% in female, respectively. Frequently noted symptoms were nonspecific including cough, sputum, fever, weight loss in the order of frequency but cough was more frequent than in pulmonary tuberculosis. Physical examination showed rale, decreased breathing sound and wheezing and wheezing was more frequent than in pulmonary tuberculosis. All 7 subtypes were noted bronchoscopically and edema-hyperemia (stenotic without fibrosis) type was most frequently(32.7%) noted, and followed by chronic nonspecific bronchitis type, stenotic with fibrosis type and actively caseating type in the order of frequency. The relationship between subtypes of endobronchial tuberculosis and radiologic findings was insignificant. Right lung was involved more frequently than left lung and left upper lobe was most commonly involved site, and followed by right upper lobe and trachea. Acid-fast bacilli(AFB) positivity in sputum and / or bronchial washing fluid was 73% and suggested high risk of infectivity. Conclusion : The frequency of endobronchial tuberculosis in patients with pulmonary tuberculosis was higher than known and also suggested bronchoscopic examination to detect endobronchial involvement should be recommanded and careful management is also needed to prevent complications.
Kim, Jae-Yeol;Lee, Jae-Cheol;Kang, Min-Jong;Park, Jae-Seok;Yoo, Chul-Gyu;Kim, Young-Whan;Han, Sung-Koo;Shim, Young-Soo;Lee, Jae-Ho
Tuberculosis and Respiratory Diseases
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v.42
no.5
/
pp.703-712
/
1995
Background: Peripheral blood monocytes are important immune effector cells that play a fundamental role in cellular immunity. In addition to their antigen-presenting and phagocytic activities, monocytes/macrophage produce a vast array of regulatory and chemotactic cytokines. Interleukin-8(IL-8), a potent neutrophil-activating and chemotactic peptide, is produced in large quantities by mononuclear phagocytes and may be an important mediator of local and systemic inflammation. Overexpression by IL-8 of such inflammation may be an important step of tissue injury frequently seen in inflammatory reaction. So it could be hypothesized that the agents which block the production of IL-8 can decrease the inflammatory reaction and tissue injury. To evaluate this, we described the effect of Dexamethasone, $PGE_2$, Indomethacin and Interferon-$\gamma$(IFN-$\gamma$) on IL-8 mRNA and protein expression from LPS-stimulated human peripheral blood monocytes(PBMC). Method: PBMC was isolated from healthy volunteers. To evaluate the effect of Dexamethasone, $PGE_2$ & Indomethacin, these drug were treated for 1 hour before and after LPS stimulation and IFN-$\gamma$ was only treated I hour before the LPS stimulation. Northern blot analysis for IL-8 mRNA and ELISA for immunoreactive IL-8 protein in culture supernatant were performed. We repeated above experiment three times for Northern blot analysis and two times for ELISA and got the same result. Results: 1) Pre- and post-treatment of Dexamethasone suppressed both the LPS stimulated IL-8 mRNA expression and IL-8 protein release in PBMC. 2) IFN-$\gamma$ pre-treatment suppressed the IL-8 mRNA expression and IL-8 protein release in unstimulated cells. 3) In LPS stimulated cells, IFN-$\gamma$ suppressed the IL-8 mRNA expression but IL-8 protein release suppression was not observed. 4) $PGE_2$ and Indomethacin exert no effect on the LPS-stimulated IL-8 mRNA and protein expression in concentration used in this experiment ($PGE_2;10^{-6}M$, Indomethacin; $10{\mu}M$). Conclusion: One of the mechanism of antiinflammatory action of Dexamethasone can be explained by the suppressing effect of IL-8 production in some extent and by this antiinflammatory effect, dexamethasone can be used to suppress local and systemic inflammation mediated by IL-8.
Background : Leukocyte-endothelial adhesion molecules have been implicated in the pathogenesis of inflammatory disease. ICAM-1, VCAM-1 and E-selectin are cell surface adhesion molecule on vascular endothelial cells. They are up-regulated by inflammatory cytokines and regulate the adhesion and migration of leukocytes across the endothelium. Tuberculosis, a granulomatous disorder is an infection caused by Mycobacterium tuberculosis. The clinical manifestations of tuberculosis are dependent on the cellular immune response to tubercule bacilli. Circulating adhesion molecules are probably formed by cleavage and release into the circulation of the extracellular domain of the membrane bound form. The elevated levels of circulating adhesion molecules have been reported in numerous disease state. To evaluate their role as markers of disease activity in tuberculosis, we measured a sE-selectin, sVCAM-1 and sICAM-1 levels in the serum with severities of mild, moderate and far advanced pulmonary tuberculosis. Methods : The control and test groups were divided as follows. Group I : control(n=5), Group II : patients with mild pulmonary tuberculosis(n=12), Group III : pateints with moderate pulmonary tuberculosis(n=20), Group IV : patients with far advanced pulmonary tuberculosis(n=19). Serum sICAM-1, sVCAM-1 and sE-selectin were measured by ELISA kit Results : Serum soluble adhesion molecules are elevated in patients with pulmonary tuberculosis, Circulating ICAM-1 levels were significantly elevated in patients with moderate and far advanced pulmonary tuberculosis when compared with control group. When compared with control group, serum sVCAM-1 levels showed significant elevation in patients with mild, moderate and far advanced pulmonary tuberculosis. Serum sE-selectin levels were significantly elevated in patients with far advanced pulmonary tuberculosis when compared with control group. Conclusion : These results suggest that sICAM-1, sVCAM-1, and sE-selectin may be invloved in the pathogenesis of tuberculosis. And, particularly, sICAM-1 and sVCAM-1 may be useful markers of the disease activity.
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