• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.119-127
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• 2013

Twenty-three strains of Leuconostoc species and 45 strains of Weissella species inhibiting the growth of Lactobacillus sakei, one of the most populous lactic acid bacteria in over-ripened kimchi, were isolated from kimchi in our previous study. Among these hetero-fermentative 68 strains, Leuconostoc mesenteroides CK0128, Weissella cibaria CK0633, and W. cibaria KK0797 exhibited a relatively high survival rate in MRS medium, which was adjusted to pH 4.3 using an acid mixture consisting of acetic and lactic acids, and produced a large amount of exopolysaccharides. The culture supernatants of 3 strains were fractionated by a molecular weight cutter and lyophilized. The fractions with a molecular weight smaller than 3,000 Da showed antagonistic activity against Staphylococcus aureus and Lb. sakei. The anti-bacterial substances were very stable to heat treatments ($121^{\circ}C$, 15 min) and active at acidic conditions below pH 5. ${\alpha}$-Amylase, lipase, and proteolytic enzymes (proteinase K and pepsin) did not affect their activities. These non-proteinaceous anti-bacterial substances inhibited the growth of several food pathogens.

• Microbiology and Biotechnology Letters
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• v.26 no.1
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• pp.50-54
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• 1998

Ethyl caproate is one of the important flavor compounds produced during the brewing of rice wine. The rice wine yeast and koji were reported to produce the esterases which synthesize and also hydrolyze ethyl caproate. From the results of monitoring the esterase activities of rice wine yeast and koji, their roles for producing ethyl caproate during brewing were postulated. In case of rice wine yeast, the production of esterase synthesizing ethyl caproate was influenced by the substrate, caproate but that of esterase hydrolyzing ethyl caproate was promoted by ethyl caproate but inhibited by caproate. The production of esterases of koji were not influenced by the substrates for ethyl caproate production but influenced by the growth of koji. The maximum concentration of ethyl caproate produced by rice wine yeast was 0.4 ppm in this research but the production of ethyl caproate by koji was not detected under our experimental conditions. Considering the results of this research, ethyl caproate is not produced by the esterases of koji during brewing but produced by the esterases of rice wine yeast. The growth of rice wine yeast represses that of koji because of the high concentration of ethanol produced by rice wine yeast. The esterases of rice wine yeast may decide the production of ethyl caproate during brewing.

• Archives of Plastic Surgery
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• v.36 no.4
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• pp.372-379
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• 2009

Purpose: The purpose of this study was to compare the effect on antibacterial activities and wound healing effect of silver containing dressings with which of Betadine against Staphylococcus aureus and Pseudomonas aeruginosa. Methods: One full thickness skin defects in rats(n=72) were developed on the back and were given rise to infection with S. aureus(n=36) and P. aeruginosa(n=36). The 72 mice were divided into 6 groups : Acticoat$^{(R)}$, Aquacel$^{(R)}$-Ag, Medifoam silver$^{(R)}$, Polymen silver$^{(R)}$, Ilvadon$^{(R)}$ and Betadine(control group) dressing groups. Five silver containing dressings and Betadine were assesed on infected wound. Measurement of wound size change, bacterial colonies count and histologic findings was applied. Antibacterial activity was analyzed with bacterial restricted zone in Mueller Hinton agar. Results: On S. aureus, wound size was more decreased in all treated groups as compared with betadine, however Ilvadon$^{(R)}$ was less decreased on P. aeruginosa. In histologic findings, experimental group showed more effective findings than others on S. aureus, however on P. aeruginosa, which was showed similar. Acticoat$^{(R)}$ was best effective in wound healing against S. aureus and P. aeruginosa. The bacterial colonies count was increased in all treated groups except Acticoat$^{(R)}$ as compared with the control group on S. aureus, which was decreased in Acticoat$^{(R)}$ and Ilvadon$^{(R)}$ group on P. aeruginosa. There were not statistical differences. The restricted zone was shown in Mueller - Hinton agar of all groups except Medifoam silver$^{(R)}$ group on S. aureus, which was shown of all groups on P. aeruginosa. There were statistical differences. Conclusion: This study suggests that silver containing dressings may have not better potential than Betadine in assisting management of wounds at risk of infection on S. aureus and P. aeruginosa. However, which have better antibacterial activity on S. aureus and P. aeruginosa.

• Korean Journal of Weed Science
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• v.4 no.2
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• pp.130-134
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• 1984

This experiment was conducted to know critical sowing depth and emergence day under different sowing depth at Crop Experiment Station, Suweon, in 1982. Tested weed species were Sagittaria pygnaea, Eleocharis kuroguwai, Cyperus serotinus and Potamageton distinetus, sowing depth of weeds were 5cm, 10cm, 15cm, 20cm and 25cm Critical emergence sowing depth of S. pygnaea, E. kuroguwai was 15cmand C. serotinus was 20cm sowing depth while P. distintus was 25cm sowing depth. Average emergence days under 5cm sowing depth for E. kurogwai, S. pygmaea, P. distintus and C. serotinus was 27.8 days, 14.1 days, 13.6 days and 7.3 days. While, relationships between sowing depth and day to emergence was positively correlated. Gernination percent of weeds under Critical emergence sowing depth, S. pygmaea, E. kurogwai and, P. distinctus was 57%, 67%, 80%, but C. serotinu.s was all died.

• Korean Journal of Weed Science
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• v.1 no.1
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• pp.21-29
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• 1981

Nationwide survey of weed population in paddy rice field was conducted from 1760 fields all around Republic of Korea in 1981. The weed species observed include 2 species of grasses, 7 species of sedges, and 18 species of broadleaf weeds, and the dominant weed species were Monochoria vaginalis, Sagitraria trifolia, Potamogeton distinstus, Sagittaria pygmea and Cyperus serotinus. More and many weeds were growing in single cropped field than double cropped field. In single cropped fields, weed population was reduced when autumn plowing was conducted. In double cropped fields, more weeds were growing in barley or wheat grown fields than vegetable gown fields. Among the five paddy soil types, more weeds were growing in saline soils and poorly drained soils than others. The proportion of annual and perennial weeds in national average was 44% and 56%, and perennials became more dominant in central part of the peninsula. Autumn plowing and double cropping appeared to reduce the perennial weed population, however, the increased perennial weed population, presumably due to continuous use of herbicides which controls mostly annuals, seemed to an urgent problem to be solved.

• The Journal of the Korean Society for Microbiology
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• v.21 no.2
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• pp.233-241
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• 1986

Patients of chronic hepatic diseases(n=107) including chronic hepatitis caused by hepatitis B virus infections(n=31), liver cirrhosis(n=53), and hepatocellular carcinoma(n=23) were examined to ascertain genetic relationship between chronic hepatic diseases and histocompatibility antigen. Peripheral blood lymphocytes were separated from whole blood by the method of Ficoll/Hypaque gradient. Total 54 histocompatibility antigens(class I antigens: 41, class II antigens: 13) were analysed by performing of complement dependent microlymphocytotoxicity method using Terasaki's and Catholic Medical College tissue typing plates. HLA antigen frequencies were compared with those of 661 normal controls. The following results were obtained: 1. HLA antigen frequencies of HLA-Bw46, -Bw76, -Cw1, -Cw6, and HLA-DR8 in chronic hepatitis patients were shown to be higher than those in controls(P<0.01). 2. HLA antigen frequencies of HLA-Bw46, -Cw7(P<0.01), and HLA-B37, -Bw58, -Cw1, -MT1(P < 0.05) in liver cirrhosis patients were shown relatively higher frequencies than those in controls. 3. In patients with hepatocellular carcinoma, antigens of HLA-A1, -A26, -Cw3, -Cw7 and HLA-DR6 were dominantly detected. 4. There were negative associations with HLA-Cw4, and -DR4 in patients of chronic hepatic diseases(P < 0.05).

• Archives of Plastic Surgery
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• v.35 no.4
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• pp.385-392
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• 2008

Purpose: The most effective methods of harvesting, preparing, and injecting autologous fat grafts have been inconsistent and conflicting. With its limitation as resorption in fat grafting, handling various techniques affect adipocyte survival, and is crucial to optimizing its long-term survival. To improve graft survival, re-implantation of cryopreserved adipocytes was developed. In addition, adipocytes do not induce immune rejection in response to non-self lymphocytes in a mixed lymphocyte reaction. The purpose of this study is to analyze the changes in cryopreserved adipocytes so as to determine the most efficient long-term storage period, and to analyze the changes in cryopreserved allografted adipocytes so as to determine the efficacy of cryopreserved adipocytes allografting. Methods: Fat tissues were harvested from the inguinal and retroperitoneal fat pad of mice. After the centrifugation of the harvested fat tissues, they were disintegrated with collagenase. The adipocytes were obtained by centrifugation of the disintegrated fat tissues. The adipocytes were treated as follows: (1) They were examined for weight and then frozen at $-20^{\circ}C$(n=25). For four months, each five frozen samples were taken and examined for weight and histologic changes in the 1st week, the 1st month, the 2nd month, the 3rd month, and the 4th month, respectively. (2) The adipocytes were immediately frozen at $-20^{\circ}C$(n=125). For four months, five frozen samples were taken, and allografted in the same time period as above. Finally, for four months, five cryopreserved allografted adipocytes were taken and examined for histologic changes in the same time period as above. Results: (1) Significant weight changes and histologic findings with inflammatory and destructive changes were observed in the cryopreserved adipocytes in three months. (2) Significant fat necrotic changes in the histologic changes with Hematoxylin and eosin stain were observed in the cryopreserved allografted adipocytes since the first week, independent of the freezing period. Conclusion: The study results show that the adipocytes that were cryopreserved for more than three months underwent obvious weight reductions and necrotic changes, and the adipocytes that were allografted without freezing were viable for four months, but the cryopreserved allografted adipocytes had obvious necrotic changes since the first week regardless of the freezing period.

• Journal of Korea Spatial Information System Society
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• v.3 no.1 s.5
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• pp.49-62
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• 2001

Recently, component-based software has become main trends in designing and developing computer software products. This component-based software has advantage of the interoperability on distributed computing environment and the reusability of pre-developed components. Also, GIS is designed and implemented with this component-based methodology, called Open GIS Component. OGC(OpenGIS Consortium) have announced various implementation and design specification and topic in GIS. In GIS, Spatial analysis functions like network analysis, TIN analysis are very important function and basically, estimate system functionality and performance using this analysis methods. The simple feature geometry specification is announced by OGC to increase the full interoperability of various spatial data. This specification includes just geometry spatial data model. However, in GIS which manages spatial data, not only geometric data but also topological data and various analysis functions have been used. The performance of GIS depends on how this geometric and topological data is managed well and how various spatial analyses are executed efficiently. So it requires integrated spatial data model between geometry and topology and extended data model of topology for spatial analysis, in case network analysis and TIN analysis in open GIS component. In this paper, we design analysis component like network analysis component and TIN analysis component. To manage topological information for spatial analysis in open GIS component, we design extended data model of simple feature geometry for spatial analysis. In addition to, we design the overall system architecture of open GIS component contained this topology model for spatial analysis.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.43 no.9
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• pp.822-829
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• 2015

In this paper, the piezoelectric-hydraulic pump with a piezostack actuator as a driving source has been designed, fabricated, and evaluated for its application to UAV's brake system. The performance requirements of the piezoelectric-hydraulic pump were decided based on the requirements analysis of the target aircraft brake system. The geometric design of the piezoelectric-hydraulic pump to meet the performance requirements of the pump was conducted, and all components of the pump including the spring sheet type check valves were machined with close tolerance. By constructing a test apparatus for the performance check of the piezoelectric-hydraulic pump, the performance characteristics of the pump, such as the outlet flow rate for load-free condition and the outlet oil pressure for closed loop condition, have been evaluated. It has been found by the performance test result that the developed piezoelectric-hydraulic pump satisfies the design requirements effectively.

• Korean Journal of Food Science and Technology
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• v.34 no.2
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• pp.311-318
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• 2002

A new bacteriocin producing lactic acid bacteria having antagonistic activity against Lactobacillus plantarum, was isolated from Kimchi. It was identified as Leuconostoc mesenteroides, and designated as Leuconostoc mesenteroides B7. The bacteriocin from Leuconostoc mesenteroides B7 named as bacteriocin B7 was stable in the pH range $2.5{\sim}9.5$. Bacteriocin B7 was active over a wide temperature range from $4^{\circ}C$ to $120^{\circ}C$. It was inactivated by proteinase K, trypsin, ${\alpha}-chymotrypsin$, and protease treatments indicating its proteinous nature. Tricine-SDS-PAGE of the purified bacteriocin B7 showed the presence of a single band, having a molecular mass of about 3,500 dalton. Mixed culture of the producer and the indicator, Lb. plantarum KFRI 464 or Lb. delbruekii KFRI 347, increased production of bacteriocin B7. This result suggested the presence of bacteriocin inducing factor in the indicator strain. The inducing factor was localized in cell debris and intracellular faction of the indicator cell, Lb. plantarum KFRI 464. Treatment of the inducing factor with proteinase K destroyed inducing activity. This result strongly suggested that the inducing factor is a protein.