• Journal of Periodontal and Implant Science
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• 제31권4호
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• pp.737-747
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• 2001

Regeneration of Periodontium with PRP does not only improve regeneration rate and density of bone but have a possibility to estimate faster healing process for soft tissue. And also, synthetic bone and xenogenic bone graft are effective on regeneration of periodontium. The purpose of this study is to evaluate the effectiveness of synthetic bone ($Biogran^{(R)}$) and xenogenic bone ($BBP^{(R)}$) grafts with the PRP technique on regeneration of periodontium. 52 Generally healthy Pt. who had pocket depth 5mm at any of 6 surfaces of the teeth were in the study at Dept. of Perio. in Dankook Dental Hospital. Open Flap was treated for 18 infra-bony pockets as control group, $Biogran^{(R)}$ with PRP was inserted for 25 infrabony pockets as first test group, and $BBP^{(R)}$ with PRP was inserted for 22 infrabony pockets as 2nd test group. Then evaluation was made after 3 and 6 months 1. 6 months after surgery, each difference of average probing pocket depth was $2.61{\pm}0.23$ for control, $3.40{\pm}0.30$ for 1st test, and $3.45{\pm}0.37$ for 2nd test group. 2. 6 months after surgery, each difference of clinical probing attachment level was $1.39{\pm}0.12$ for control, $2.88{\pm}0,24$ for 1st, and $2.86{\pm}0,27$ for 2nd test group. 3. 6 months after surgery, each difference of Maximal probing attachment level was $1.11{\pm}0.16$ for control, $3.28{\pm}0.30$ for 1st, and $3.27{\pm}0.35$ for 2nd test group. 4. There were significant differences for clinical change of each three group which were between average probing pocket depth and clinical attachment level of 3,6 months and minimal and maximal attachment level after 6 months 5. There were significant differences for average probing pocket depth which were only at control group and 2nd test group between 1 and 6months. For clinical attachment level and minimal and maximal proving attachment level, there was a significant difference after 6month of surgery. 6. There was no significant difference between two test groups for average probing depth, clinical attachment level, and minima1 and maximal probing attachment level. As the result, PRP with bone graft is very effective for regeneration of periodontium and there is no difference between xenogenic bone and synthetic bone.

• 대한골관절종양학회지
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• 제4권1호
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• pp.13-21
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• 1998

Taxol이 악성 골종양 세포에 어느 정도의 세포독성이 있는지를 평가하기 위해 한국세포주 은행에서 분양 받은 G-292, SaOS-2 및 HT-1080의 3가지 악성 골종양 세포들을 대상으로 기존의 항암제인 methotrexate, adriamycin, ifosfamide, cisplatinum과 함께 각각 투여하여 MTT분석법으로 정량 및 비교 분석하였으며, adriamycin과 taxol을 병용 투여하여 항암제의 상호작용을 isobologram 분석법으로 조사하여 다음과 같은 결과를 얻었다. 1. Taxol의 악성 골종양 세포 주들에 대한 $IC_{50}$는 G-292에서는 $2.7{\times}10^{-2}{\mu}g/ml$, $SaOS^{-2}$에서는 $1.0{\times}10^{-2}{\mu}g/ml$, $HT{\times}1080$에서는 $1.1{\times}10^{-3}{\mu}g/ml$이었다. 2. Taxol은 악성 골종양 세포주들에 대해 기존의 항암제들 보다 강한 세포독성을 보였으며, 기존 항암제의 세포 독성의 강도는 adriamycin이 제일 높은 역가를 보였으며 그 외 methotrexate, cisplatinum, ifosfamide순이었다. 3. Taxol과 adriamycin을 병용 투여하여 상호작용을 관찰한 결과 G-292와 SaOS-2 세포 주에서 상승효과가 관찰되었으며, HT-1080에서는 상승효과가 관찰되지 않았다.

• 대한골관절종양학회지
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• 제3권1호
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• pp.32-38
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• 1997

PURPOSE : For the reconstruction of large bone defect after tumor resection, it is possible to reuse the bone involved by tumor with some treatment to it. Several bone-reusing methods have been reported such as autoclaving, low-heat treatment(pasteurization) and intraoperative radiotherapy. We have used extracorporeally radiated autogenous bone graft for reconstruction after tumor resection, and analyzed the periods for junctional union, functional results and complications to know the indications of this method. METHODS : From Dec. 1993 to Sept. 1995, nine patients had taken autogenous bone graft with extracorporeal irradiation. Eight cases were osteosarcoma and 1 giant cell tumor. The graft sites were 5 in femur, 3 proximal tibia and 1 femur and tibia. Stage 3 was 1 case(GCT), Stage IIB 3 and Stage IIIB 5. After wide resection, surrounding soft tissue and intramedullary and extramedullary portion of the tumor were removed. Radiation was done in 5000cGy to the resected bone. Ender nails and bone cement were inserted and filled into the medulla to prevent fracture. RESULTS : Average follow-up period was 12.3(4 to 21) months. Average junctional union period in simple X-ray was 6.5 months in 4 cases. Average functional score following Enneking's criteria was 19(12-27). Complications were as follows ; condylar fractures and femur neck fracture in 4 cases, subluxation of the knee joint 3 and infection 1. Although local recurrence was detected in 1 case, the site of recurrence was not in the radiated bone but surrounding soft tissue. At final follow-up, no recurrence was found in one case(GCT), CDF 2, AWD 2, DOD 3, and died of chemotherapy related sepsis 1. CONCLUSIONS : Extracorporeally radiated bone autograft is considered to be a method for reconstruction of the large bone defect made by tumor resection, especially in the reconstruction around the joint.

• 한국결정성장학회지
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• 제32권5호
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• pp.191-198
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• 2022

치과용 임플란트 재료로 주로 사용되는 지르코니아 및 티타늄 합금은 생체불활성 특징으로 인하여 골유착 및 골형성 능력이 떨어진다. 이러한 문제를 쉽고 간단하게 해결하기 위한 방법으로는 생체활성 물질을 표면에 코팅하여 생체 활성을 높이는 방법이 있다. 본 연구에서는 우수한 골결합 능력을 가진 실리케이트계 세라믹인 아커마나이트(Ca₂MgSi₂O₇)를 고상반응법으로 합성하고, SBF 용액 내 침적실험을 통하여 합성 아커마나이트 분말의 생체활성을 분석하였다. 고상반응 출발원료로는 탄산칼슘(CaCO₃), 탄산마그네슘(MgCO₃), 이산화규소(SiO₂) 분말을 사용하였다. 분말을 혼합 및 건조한 후, 가압 성형하여 디스크 형태로 만든 후, 고상반응 온도를 변화시키며 아커마나이트 상의 합성을 유도하였다. 합성된 아커마나이트 펠릿의 용해 및 생체활성 분석을 위하여 SBF 용액 내 침적 시키고, 침적시간에 따라 아커마나이트의 표면 용해 및 하이드록시아파타이트 석출을 분석하였다. 합성반응 온도가 높아질수록 아커마나이트 상이 뚜렷하게 나타난 반면에, SBF 용액 내 용해는 천천히 진행되었다. 합성된 아커마나이트 분말의 생체활성도는 대체적으로 우수하였으나, 그 중에서도 1100℃에서 고상반응 하여 합성한 분말에서 적절한 용해 및 하이드록시아파타이트 입자의 석출이 잘 일어나는 것으로 분석되었다.

• Journal of Korean Neurosurgical Society
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• 제30권11호
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• pp.1284-1290
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• 2001

Objective : PCB cervical instrument is a newly introduced fusion device which comprises cervical plate, cage (spacer) and screw system. It is developed to enhance fusion rate and stability, as well as to reduce complications related to hardware failure. We investigated the efficacy of clinical and radiological results of PCB instrument and Lubboc bone. Methods : From August, 1998 to October, 1999, authors performed 54 cases of anterior cervical interbody fusion with PCB instrument and Lubboc bone. Retrospective analysis was undertaken based on clinical and radiological findings. Clinical improvement was evaluated according to Odom's criteria. Cervical plain films and tomography were taken every 2 months to evaluate the degree of interbody fusion and disc height changes. Results : Bone fusion was observed in 36 cases(90%) over 6 months after operation, and during which time there was no significant interval change. There were 3 cases of hardware dislodgement. Disc height was increased significantly and preserved in all cases. Clinical outcome over good degree was seen more than 94% of patients. Conclusion : Longer follow-up period and comparative studies to similar instrument appear to be necessary, but this instrumentation system has shown high fusion rate and fewer adverse effects in our series. We believe this system might be indicated for the treatment of cervical trauma and degenerative disease in selective cases.

• Journal of Periodontal and Implant Science
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• 제30권2호
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• pp.277-287
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• 2000

For the regeneration of osseous defect on the furcation area, autogeneous bone graft has been primarily used. But it has the limitation of donor site, additive surgical operation etc. Recently anorganic xenogenic bone graft materials of removing all organic components are commonly used for the regeneration of periodontal defects. This study was the comparison of the effect on the regeneration with two types xenografts($Bio-oss^{(R)}$ and Ca-P thin coated Bovine bone powder) on the furcation involvement in Beagle dogs. After surgically induced chronic periodontitis in bifurcation area of premolar, $Bio-oss^{(R)}$ and Ca-P BBP were grafted on the osseous defects. Tissue blocks including defects with soft tissues were harvested following a four-& eight-week healing interval and prepared for histologic analysis. The results of this study were as follows: 1. $Bio-oss^{(R)}$ group: there were significant differences among the $Bio-oss^{?}$ group at 4weeks and 8weeks, but the control group had various appearances : new bone formation, resorption of graft materials by multinuclear giant cells, connective tissue cells intervention in the bone graft sites etc. 2. Ca-P BBP group: lots of new bone formation were observed but the arrangement of periodontal ligament was not completed at 4weeks. New bone were replaced mature bone and the periodontal ligaments showed the functional arrangement at 8weeks. 3. By reason of undergrowing the epithelium within the osseous defects, new bone formation was not happened in the upper area of bifurcation in $Bio-oss^{(R)}$ group. 4. In Ca-P BBP group, epithelial undergrowth was not seen and generally showed much more new bone formation. 5. Ca-P BBP group showed the osteocyte-like cells at the inner portion of the graft materials 6. Both groups were similar to resorptive appearances of graft materials, but Ca-P BBP group had the better effects of osteoconduction.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제28권3호
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• pp.205-215
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• 2002

The purpose of this study was to evaluate the tissue response in various bone grafting materials, especially xenogenous bone materials in vivo, compare of bone formation capacity of various bone grafting materials on rat skull defects and evaluate the effect of Hyaluronic acid on healing of human Demineralized Freezed Dried Bone allogenous graft (DFDBA) materials in rat calvarial defects. 30 Sprague-Dawly rats were divided into 4 groups. $7{\times}7mm$ size bony defect were artificially prepared in the calvaria (both parietal bone) of all 30 rats and follwed group grafting of autogenous bone graft on right side and allogenic DFDBA on left side bone graft (rat DFDB) in 15 control group, but in 15 experimental group, xenograft (human DFDB) on left side, hyaluronic acid treated with xenograft on right side. Sequential sacrifices was performed at 1, 2, 4, 6, 8 weeks of experiment. These specimens were stained with H&E and MT stain, and then histologic analysis under light microscope was carried out. There were inflammatory reaction in all graft material during early stage. Autogenous and Allogenous DFDBA graft group observed inflammatory reaction at 1 week. Xenograft group persistant inflammatory reaction until 4 weeks, but in HA treated xenograft group inflammatory reaction was decreased at 2 weeks. Osteoblastic activity in control group was begun at 2 week, xenograft group was delayed at 6 weeks, however HA treated xenograft group was begun at 4 weeks. At 2 week, mild osteoclastic activity were observed in all xenograft group not in concerned to HA, but there was no difference each group after 4 weeks. There are most activated angiogenesis around graft mateirals in xenograft group at 2 weeks, but in HA treated xenograft group, decreased angiogenesis was observed at same time. Bone formation and bone maturation of xenograft group, there was no difference in HA treatment, was less than control group. Fibrosis around xenograft materials were observed until 6 weeks, there was no difference between xenograft and HA treated groups.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제21권1호
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• pp.13-22
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• 1999

The purpose of this investigation was to evaluate the acceptability of the collagen-based xenograft ($Laddec^{(R)}$). Full thickness bone defects were prepared in the calvaria of the rats. In the experimental groups the bone defects were filled with a kind of collagen based xenograft. And bone defects, which left without filling, were used as control groups. Sequential sacrifice was performed at the 1st, 2nd, 4th, 8th, and 16th weeks of experiment. 1. At the 1st week of experiment, infiltration of chronic inflammatory cell was observed in all groups. In the experimental group, resorption of the xenograft was initiated. 2. At the 2nd week of experiment, infiltration of chronic inflammatory cells was decreased in all groups. In the experimental group, active resorption of xenograft and new bone formation from the periphery of the xenograft was observed. 3. At the 4th and 8th weeks of experiment, more resorption of the xenograft and new bone formation with calcification was observed in the experimental group. 4. At the 16th week of experiment, small bone trabecula was formed partially in the control group but that couldn't fill the whole bone defect. In the experimental group, more advanced resorption of xenograft and more new bone formation was observed. However mid portion of the xenograft was still remained without resorption. 5. From this experiment, we concluded that the collagen-based xenograft had some osteoconductive but no osteoinductive property. So the xenograft would be used for the bone defect filling material where rapid bone remodeling is not required.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제30권6호
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• pp.554-560
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• 2008

In posterior maxilla, it is difficult to achieve primary stability of implants due to sinus pneumatization, alveolar bone loss, and low bone quality. The accurate and objective primary stability assessment is important for good prognosis of implants. Purpose: The aim of this study was to assess the primary stability of the non-submerged, internal type implants with maxillary sinus augmentation using deproteinized bovine bone mineral by a resonance frequency analyzer, when residual alveolar bone height is under 8mm Materials and methods: A total of 20 implants was placed into 5 grafted maxillary sinuses in 5 patients. Deproteinized bovine bone mineral (Bio-$Oss^{(R)}$) was used as graft material. SS II implants (diameter 4.1mm, and length 11.5mm, SLA suface)) were placed. All of the patients received maxillary sinus graft procedure by 1-step technique. Residual bone height was $1.3{\sim}7.8mm$ (mean 4.4mm) measured by panorama radiography. After implant placement, RFA was measured at 4,8,12,20 weeks. The results were divided into 2 groups; RFA value under 4mm and over 5mm of bone height. It was statistically analyzed. Results: 1. The primary stability of implants was increased with time 2. The RFA value was above 65 ISQ at 12 weeks 3. There was no correlation between RFA and residual alveolar bone height in maxillary sinus augmentation by 1-step technique. Conclusion: 1-step surgical procedure is a feasible option for patients with as little as 4mm residual alveolar bone height, when utilizing non-submerged, internal type implants with xenografts.

• 대한방사선기술학회지:방사선기술과학
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• 제37권4호
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• pp.279-286
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• 2014

유방암 환자의 외과적 수술과 방사선 및 화학적 치료 전 후의 골밀도 수치를 비교함으로써 암 치료에 의한 골소실 문제의 심각성을 도출하여 고 위험군선별 및 골다공증예방을 위한 기초자료를 제공하고자 하였다. 2007년 3월부터 2013년 9월까지 유방암으로 진단을 받고 수술 및 치료를 받은 후 골밀도 검사를 시행한 환자 254명을 대상으로 하였다. 이중 폐경에 이르렀거나 자궁적출술을 받아 폐경된 84명을 제외한 171명을 최종 분석의 대상으로 하였다. 방사선흡수법에 의한 골밀도 영상(요추부위와 대퇴골부위)에서 치료 전 후 측정된 골밀도 수치를 분석하였다. 치료 전 후의 전체적인 골밀도 변화량과 치료유형별 변화량을 비교하였으며, 환자의 결혼 유무, 자녀수, 수유유무, 초경나이, 유방암 치료형태 등을 변수로 하여 세부적인 차이점 및 연관성을 분석하였다. 측정된 자료는 SPSS for Windows Program(Version 18.0)을 사용하여 통계 분석하였다. 요추에서는 치료 후 평균 7.1% 감소하였고, 대퇴골에서는 평균 3.1% 감소하였으며, 통계적으로 매우 유의한 차이를 보였다(p<.01). 또한 화학적 치료를 시행한 환자군의 대퇴골 부위에서 치료 및 수술 후 $0.067g/cm^2$의 상대적으로 큰 골밀도 수치의 감소량을 보였으며 통계적으로 유의하였다(p<.05). 그 외 결혼 유무, 자녀수, 수유 유무, 초경 나이 등에 따라서는 감소량의 차이가 있었지만 통계적 수준에서 유의성은 없었다. 폐경 전 유방암 환자의 치료 후 골밀도 수치 감소를 보임에 따라 감소량이 상대적으로 큰 환자를 고 위험군에 포함시켜야 할 것이며, 이를 토대로 적극적인 예방 정책이 필요하리라 사료된다.