• Proceedings of the Korean Society of Fisheries Technology Conference
• /
• 1996.06a
• /
• pp.398-401
• /
• 1996

• Journal of the Korean Chemical Society
• /
• v.52 no.5
• /
• pp.588-592
• /
• 2008

• Proceedings of the Korea Society for Simulation Conference
• /
• 2003.06a
• /
• pp.155-160
• /
• 2003

Today, for improvement of container terminal productivity, it is necessary to develop ACT(Automated Container Terminal) But, decision rules for conventional container terminals expose various limitations to be applied to ACT. Specially it is important fact to assignment of containers, because the character of ACT which block layout is vertical for berth. And then there is need which assignment of containers with remarshaling work using ATC(Automated Transfer Crane)s. The objective of this study is to provide the operation rules for remarshaling work in ACT. To analyze the proposed rules, the simulation is performed, and we show efficiency of remarshaling on various remarshaling operation rules.

• Proceedings of the Korea Inteligent Information System Society Conference
• /
• 2004.11a
• /
• pp.287-295
• /
• 2004

컨테이너 터미널의 적하 작업은 사전에 계획된 순서대로 컨테이너를 장치장(yard)에서 하나씩 반출하여 선박에 선적하는 작업이다. 만일 현재 반출하고자 하는 컨테이너의 상단에 다른 컨테이너들이 장치되어 있다면, 부득이하게 위에 놓여 있는 컨테이너들을 다른 곳으로 옮겨야 하는 재취급(rehandling)이 발생하고 이로 인하여 반출 작업은 지연된다. 본 논문에서는 적하 계획이 수립되어 장치장의 한 베이(bay)내 컨테이너들의 반출 순서가 결정되었을 때, 반출 시작 시점까지 남는 유휴 시간을 활용하여 재취급이 발생하지 않도록 컨테이너들을 재정돈(이적, 리마샬링)하는 방안을 제안한다. 본 제안 방안은 분지 한계법(branch and bound)을 적용하여 컨테이너 이동 계획을 탐색하며, 생성된 해는 최소의 컨테이너 이동 횟수가 보장된다. 4 단 6 열과 5 단 9 열의 장치장 구조를 대상으로 실험한 결과 99% 이상의 상황에서 허용할 수 있는 시간 내에 계획을 수립할 수 있음을 확인하였다.

• Journal of Veterinary Clinics
• /
• v.12 no.1
• /
• pp.905-910
• /
• 1995

GC 함유량(72%)이 높은 Mycobacterium paratuberculosis의 DNA의 PCR 증폭시 특이성과 생산성을 높이기 위하여 PCR 반응액에 denaturant인 DMSO, glycerol, formamide, Tween 20과 NP 40를 첨가하였다. Denaturant를 첨가하지 않은 상태의 PCR에서는 다수의 비특이적인 DNA가 관찰되었으며 표적 DNA 생산량이 낮았다. 모든 denaturant는 PCR의 특이성과 생산물의 생산량을 증가시했으며, 이들 중 DMSO, glycerol, farmamide와 NP 40는 높은 농도에서 생산량을 증가시켰다. Tween 20은 낮은 농도에서 생산량을 증가시켰다. Denaturant를 첨가하였음에도 불구하고 대부분의 반응에서 1 또는 2개의 비특이적인 DNA가 관찰되었다.

• Parasites, Hosts and Diseases
• /
• v.33 no.2
• /
• pp.101-106
• /
• 1995

Soluble protein of purified Pneumocvstis cnrinii was prepared from experimentally infected rats. SDS-PAGE of the crude antigen resolved about 20 protein bands from 20 to 200 kDa. Out of thenl, 116 kDa band strongly reacted and 45-55 and 100 kDa bands reacted weakly to the positive reference human serum from U.S.A. Western blot analysis with sera of 130 normal children and 15 newborns in Korea revealed specific IgG antibody reaction to 40-55 and 116 kDa protein bands. Forty percent (40.0%) of the 145 sera were positive with any of the antigenic protein bands of R corinii. The positive rate was 56% in 50 males and 33.3% in 48 females. The protein bands 40-55 and 116 kDa from rat P. carinii were confirmed to cross-react with human sera in Korea.

• Parasites, Hosts and Diseases
• /
• v.35 no.2
• /
• pp.87-94
• /
• 1997

Clonorchis sinearis is a liver fluke which is the most prevalent helminth of humans in Korea. The better diagnostic measure of clonorchiasis is required for its nationwide control program. The present study observed antigenic bands of C. sinensis and reacting immunoglobulins in serum of infected residents. Adult C. sinensis were recovered from experimentally infected rabbits and soluble crude extract of the worms was used as the antigen after supplementation of E-64, a cysteine proteinase inhibitor. SDS- PAGE of the crude antigen resolved more than 20 protein bands between 200 and 14 kDa. The sera of infected humans collected at an endemic village showed specific IgG and IgE antibodies but little IgM and IgA antibodies. The protein bands of 94, 80, 72, 68, 52, 47, 43, 37, 34, and 28-25 kDa strongly reacted with serum Ig(GMA) or IgG antibody and 64, 62, 52, 47,44, 34,28, and 26 kDa bands reacted with serum IgE antibody. However, the 94, 80, 72, 68, 64, 62, 52, 47, and 40 kDa bands of C. sinensis antigen were found non specific. The protein bands of 43, 34, and 28-25 kDa of C. sinensis are primary target molecules of further analysis.

• Journal of Navigation and Port Research
• /
• v.29 no.1 s.97
• /
• pp.83-90
• /
• 2005

Intra-block remarshalling is the task of rearranging into some target bays those containers which are scattered around within the block, so that the containers can be loaded onto the ship efficiently. However, if we rearrange the containers without considering the container loading sequence, excessive rehandling work will be required at the time of loading because the containers to be fetched are often stacked under other containers. Therefore, the remarshalling should be done by moving the relevant containers in an appropriate order. This paper presents an efficient heuristic search technique for finding an appropriate container moving order during remarshalling to avoid rehandling at the time of loading. Simulation experiments have shown that the proposed method can generate rehandling-free solutions in real time.

• Applied Biological Chemistry
• /
• v.40 no.6
• /
• pp.551-555
• /
• 1997

This experiment was carried out to elucidate the biological activity and absorption characteristics of flavonoids in Rhizobium and Bradyrhizobium and to obtain basic information on host specific nodulation by flavonoids in rhizobium-legume symbiosis. The purpose of the present study was to explore the biological activity and the flavonoid absorption indicates that host-specificity is induced by flavonoids in symbiotic nitrogen fixation. Biological activity increased by daidzein and genistein treatment on B. japonicum KCTC 1539 whereas decreased by luteolin treatment but increased by luteolin treatment on R. meliloti whereas decreased by daidzein and genistein treatment. Daidzein and genistein are absorbed by B. japonicum, KCTC 1539 at higher rate than other flavonoids. Especially, luteolin was absorbed at a least rate. Luteolin are absorbed by R. meliloti KCTC 2353 at higher rate than other flavonoids. Especially, daidzein and genistein was absorbed at a least rate.

• Journal of the Korean GEO-environmental Society
• /
• v.10 no.1
• /
• pp.15-23
• /
• 2009

The aim of this study was an evaluation of closed non-sanitary landfill's stabilization degree and a determination of its an optimal reclamation method. In order to evaluate the stabilization degree, physical compositions of landfill wastes in 21 closed non-sanitary landfills were analyzed. There were 4 major items such as cover soils, organics, combustibles and incombustibles. With respect to the results of physical compositions, it was determined that the waste in 9 sampling sites of closed non-sanitary landfills after 10years of the relief time was not fully stabilized. The closed non-sanitary landfills must be reclaimed as soon as possible. The main material in closed non-sanitary landfills was cover soils and the highest content was 89.96%. Otherwise, the contents in sanitary landfills was small and 9.89~11.12%. Therefore, it was evaluated that the recovered soil by sorting transfer treatment could be reused as on-site cover soils of the reclamating non-sanitary landfills and/or constructing materials.