• Journal of the Korea Organic Resources Recycling Association
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• v.20 no.4
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• pp.75-85
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• 2012

This study was investigated to develop an alternative medium for the cultivation of oyster mushrooms. The cultivation characteristics for major cultivated varieties of oyster mushrooms were campared with the food waste medium. In addition, water control & a gap formation of food waste is being pointed out as a problem of the medium material. Therefore, certain amounts of earthworm casting were supplied to the food waste medium to overcome the water and compaction phenomenon. This study investigated the use of the total 6 cultivars of oyster mushrooms, the Suhan(PL.1), Chiak No. 5(PL.2), Chiak No. 7(PL.3), Samgu No. 5(PL.4), Samgu No. 8(PL.5), Jangan No. 5(PL.6). In terms of medium, the optimum growth of the mycelium of the oyster mushroom was observed in the beet pulp medium but in food waste, the mycelium's growth was observed as insufficient in the bulk of cultivars. The Jangan No. 5(PL.6) was superior to other cultivars relative to the mycelium's growth. The optimum concentration of earthworm casting added the foodwaste medium was 30%. When Jangan No. 5(PL.6) was incubated in the foodwaste meidum containing 30% earthworm casting, the early days to germination were long, almost double the amount of time than that of the beet pulp medium. However, it was found that the fruit body size was larger than that of the beet pulp by up to 20.5%, and the length of the fruit body was up to 6.2% longer than that of the beet plup. The total yields increased by 60.5%. The analysis results of the oyster mushroom's nutritional contents cultured using the food waste medium, shows that the protein, vitamin A and vitamin E increased 70.6%, 2.4%, 0.8% respectively, the fat decreased by 12.6% and the oleic acid increased in a very small amount compared to that of the beet pulp medium.

• Journal of Life Science
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• v.15 no.3 s.70
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• pp.479-485
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• 2005

The 994 throat swabs obtained from 688 adults and 306 children patients with respiratory diseases were examined for Mycoplasma pneumoniae infection by culture method. Antimicrobial susceptibilities of the resulting 123 M. pneumoniae isolates were evaluated by testing minimum inhibitory concentrations (MICs) of erythromycin, minocycline, tetracycline, josamycin, sparfloxacin, ofloxacin, and ciprofloxacin by a broth micro-dilution method. The erythromycin resistant strains of M. pneumoniae was determined above $1.0{\mu}g/ml$ of MIC for erythromycin. The erythromycin resistant strains of M. pneumoniae was confirmed resistant gene mutation of the portions of genes 23S rRNA (domain II and V), and ribosomal protein 14 and L22 by PCR amplified and their nucleotide sequenses were compared to those of the susceptible strain M129. The isolation rate of M. pneumoniae was $12.9\%$ (89/688) for the adults and $11.1\%$ (34/306) for the children. The $MICs_{90}$ of the M. pneumoniae isolates were $0.12{\mu}g/ml$ for minocycline, $0.25{\mu}g/ml$ for sparfloxacin, $0.5{\mu}g/ml$ for ciprofloxacin, ofloxacin, and tetracycline, respectively, and $2.0{\mu}g/ml$ for josamycin and erythromycin, respectively. The isolation rate of erythromycin resistant M. pneumoniae from patients was $49.4\%\;(44/89)$ for the adults, $47.1\%\;(16/34)$ for children, and $48.8\%\;(60/123)$ for the total. No mutation could be detected in the ribosomal protein L22 region, but all strains were mutated in the ribosomal protein L4 as two point mutation M144V. Two point mutations in domain V of 23S rRNA were selected in the presense of erythromycin resistant M. pneumoniae isolates, such as one strain was G2057C mutant, two strains were A2059C mutants, three strains were C2611G mutants, four strains were A2058C mutants, five strains were A2058T mutants, twenty strains were A2059G mutants, and twenty-five strains were A2058G mutants, respectively. These results show that erythromycin was not the most active compound against M. pneumoniae infection in Korea and clinical studies of macrolides in human patients are demanded.

• Korean Journal of Food Science and Technology
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• v.30 no.1
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• pp.151-160
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• 1998

Tangerine peel is mostly discarded as waste in citrus processing. However, tangerine peel contains besides dietary fibers bioflavonoids such as naringin and hesperidin which act as antimicrobials and blood pressure depressants, respectively. A continuous membrane separation process was optimized for the production of bioflavonoids relative to feed flow rate, transmembrane pressure, temperature, and pH. The tangerine peel was blended with 7.5 times water volume and the extract was prefiltered through a prefiltration system. The prefiltered extract was ultrafiltered in a hollow fiber membrane system. The flux and feed flow rate didn't show any apparent correlation, but we could observe a mass-transfer controlled region of over 8 psi. When temperature increased from $9^{\circ}C\;to\;25^{\circ}C$, the flux increased about $10\;liters/m^2/min\;(LMH)$ but between $25^{\circ}C\;and\;33^{\circ}C$, the flux increased only 2 LMH. At every transmembrane pressure, the flux of pH 4.8 was the most highest and the flux at pH 3.0 was lower than that of pH 6.0, 7.0, or 9.0. Therefore, the optimum operating conditions were 49.3 L/hr. 10 psi, $25^{\circ}C$, and pH 4.8. Under the optimum conditions, the flux gradually decreased and finally reached a steady-state after 1 hr 50 min. The amount of dietary fibers in 1.0 g retentate in each separation step was analyzed and bioflavonoids concentration in each permeate was measured. The contents of total dietary fiber in the 170 mesh retentate and soluble dietary fiber in the prefiltered retentate were the highest. Naringin and hesperidin concentration in the permeate were $0.45{\sim}0.65\;mg/g\;and\;5.15{\sim}6.86\;mg/g$ respectively, being $15{\sim}22$ times and $79{\sim}93$ times higher than those in the tangerine peel. Therefore, it can be said that PM 10 hollow fiber membrane separation system may be a very effective method for the recovery of bioflavonoids from tangerine peel.

• The Korean Journal of Pesticide Science
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• v.15 no.4
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• pp.463-470
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• 2011

This study was carried out to survey the residual characteristic of chlorpyrifos and estimate their residues in squash before harvest. The pesticide was sprayed onto the crop at the recommended and its double rates 10 days before the prearranged harvest and sampling was done at 0, 2, 3, 5, 6, 7 and 10 days after spraying. The amounts of the chlorpyrifos residue in the crop was analyzed by chromatographic method. Limit of detection (LOD) of chlorpyrifos was 0.005 mg/kg and its recovery ranged from 95.21 to 102.69%. The initial concentration of chlorpyrifos sprayed with recommended dose exceeded its MRL of 0.1 mg/kg but its concentration was less than its MRL 10 days after application. However its concentration in case of the double dose was over its MRL both immediately and 10 days after application. Biological half-lives of chlorpyrifos sprayed onto squash was 2.5 and 2.9 days at the recommended and double doses, respectively. Ten days later, the residual concentration of chlorpyrifos in squash was decreased substantially. The concentration of chlorpyrifos was estimated in squash at the given day using its regression equations. The estimated concentration of chlorpyrifos in case of application with recommended dose was below its MRL at 10 days after application but its concentration in case of application with double dose was over its MRL at 10 days of the prearranged harvest. The rate of the estimated daily intake (EDI) of chlorpyrifos to its acceptable daily intake (ADI) was 282% right after application but it decreased to less than 18% at 10 days of the prearranged harvest.

• The Korean Journal of Pesticide Science
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• v.12 no.2
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• pp.148-154
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• 2008

This study was carried out to investigate the residue patterns of two insecticides, lambda-cyhalothrin and deltamethrin, commonly used for lettuce, under greenhouse conditions. The pesticides were sprayed with dilution of recommended and doubled doses onto lettuce. Their detection limits were $0.001\;mg{\cdot}kg^{-1}$ and mean recoveries at the fortification levels of 0.2 and $1.0\;mg{\cdot}kg^{-1}$ were from 101.17 to 104.25 and from 99.70 to 103.77%, respectively. The pesticides were gradually decreased in lettuce with time. Biological half-lives of lambda-cyhalothrin and deltamethrin were 1.7 and 1.4 days at the recommended dose and 1.8 and 1.4 days at the doubled dose, respectively. Initial residue amounts of lambda-cyhalothrin and deltamethrin at the recommended and doubled doses exceeded their MRLs. However, the residue levels of the pesticides in the crop sampled at harvest were less than their MRLs. The ratios of the estimated daily intake (EDI) to acceptable daily intake (ADI) by intake the crop harvested 10 days after spraying were less than 1% of their ADIs.

• Sleep Medicine and Psychophysiology
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• v.12 no.1
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• pp.27-31
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• 2005

Objectives: We attempted to compare the performance of 2 commercially available actigraphies with focus on sleep parameters, using polysomnography as standard comparison tool. Methods: Fourteen normal volunteers (5 males and 9 females, mean age of $28{\pm}4.6\;years$) participated in this study. All the participants went through one night of polysomnography, simultaneously wearing 2 different kinds of actigraphies on each wrist. Polysomnographic and actigraphic data were stored, downloaded, and processed according to standard protocols and then statistically compared. Results: Both $ActiWatch^{(R)}$ and $SleepWatch^{(R)}$ tended to overestimate the total sleep time, compared to the polysomnography. $SleepWatch^{(R)}$ tended to underestimate the sleep latency. The two actigraphs and the polysomnograph did not show significant difference of sleep efficiency, when compared with one another. In addition, all of the sleep parameters from the instruments showed linear correlations except in $SleepWatch^{(R)}'s$ sleep latency. The sleep parameters from the two actigraphs did not show much noteworthy difference, and linear relationships were found between the sleep parameters from the two actigraphs. There was no significant distinction in the results of the two different actigraphs. Conclusion: The results of two actigraphies can be used interchangeably since the sleep parameters of the two different actigraphies do not show significant differences statistically. Overall, it is not legitimate to use actigraphy as a substitute for polysomnography. However, since sleep parameters except sleep latency show linear correlations, actigraphy might possibly be used to follow up patients after polysomnography.

• Journal of the Korean Chemical Society
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• v.53 no.6
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• pp.742-748
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• 2009

In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. We described the development of DNA markers to discriminate between Korean beef cattle (Hanwoo), Holstein, and mixed cow beefs. As most breeds are standardized for coat colour, the melanocortin 1 receptor (MC1R) gene, involved in the regulation of eu/pheomelanins synthesis, has been suggested as marker for breed traceability of products of animal origin. We also designed sex-determining region Y (SRY) gene specific primers for Y chromosome detection. In this study, fragments of MC1R gene and SRY gene were amplified by multiplex-PCR and subjected to digestion by MspA1I restriction endonuclease. Reaction products were analysised by denaturing high performance liquid chromatography (DHPLC). As a result, we identified 6 DHPLC peak types from MC1R gene and SRY gene analysis. DHPLC method showed more sensitive than RFLP method for DNA fragments analysis. Therefore, DHPLC method can apply to identify for Hanwoo, Holstein and mixed beef.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.4
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• pp.309-320
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• 2017

In this study, the antioxidative effects and component analysis of 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from Annona muricata leaf were investigated. Free radical scavenging activities were performed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, total antioxidant capacities were estimated using luminol-dependent chemiluminescence assay and $^1O_2$ quenching effects were estimated. Free radical scavenging activities ($FSC_{50}$) of 50% ethanol extract, ethyl acetate fraction and aglycone fraction were 45.6, 29.8 and $18.0{\mu}g/mL$, and total antioxidant capacities ($OSC_{50}$) were 4.4, 1.1 and $2.8{\mu}g/mL$, respectively. As a result of $^1O_2$ quenching experiment, ethyl acetate and aglycone fraction showed similar activities to L-ascorbic acid used as a comparative control. The cellular protective effects of 50% ethanol extract on the $^1O_2-induced$ cellular damage of human erythrocytes were exhibited at concentration-dependent ($5-50{\mu}g/mL$). TLC and HPLC were used to analyse components in the ethyl acetate fraction and aglycone fraction of Annona muricata leaf. In ethyl acetate fraction, rutin (quercetin-3-O-rutinoside), kaempferol-3-O-neohesperidoside, nicotiflorin (kaempferol-3-O-rutinoside), p-coumaric acid were identified. In aglycone fraction, kaempferol was identified. These results suggest that the extracts of Annona muricata leaf have the applicability as antioxidant cosmeceutical ingredients.

• Korean Journal of Environmental Agriculture
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• v.27 no.1
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• pp.92-98
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• 2008

This experiment was carried out to investigate the residue patterns of two insecticides, indoxcarb and thiamethoxam, commonly used for Chinese cabbage, under greenhouse conditions. The pesticides were sprayed onto Chinese cabbage at the recommended dose and double of the recommended dose 10 days before the prearranged harvest and then sampling was done at 0.17, 1, 2, 3, 4, 5, 6, 8, 10, and 12 days after spraying. The amounts of their residues in the crop were analyzed with an HPLC. The limit of detection(LOD) of both indoxacarb and thiamethoxam was 0.01 mg $kg^{-1}$ and mean recoveries of indoxacarb and thiamethoxam were from 97.91 to 104.36% and from 97.07 to 105.49%, respectively. Half-lives of indoxacarb and thiamethoxam were 3.4 and 2.3 days at the recommended dose and 3.3 and 3.5 days at the doubled dose, respectively. The ratios of the EDI to ADI by intake the crop harvested 10 days after spraying were less than 4% of their ADIs.

• The Korean Journal of Pesticide Science
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• v.11 no.3
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• pp.154-163
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• 2007

In order to elucidate the residual characteristics of some pyrethroid insecticides commonly used for Korean cabbage, such as bifentbrin, lambda-cyhalothrin and deltametbrin, the test pesticides were sprayed onto the crop at recommended rate and doubled rate of the recommendation. Their detection limits were 0.004 mg $kg^{-1}$ and mean recoveries at the fortification levels of 0.04 and 0.2 mg $kg^{-1}$ were from 95.16 to 99.32 and from 86.81 to 103.73%, respectively. Half-lives were from 2.5 to 3.6 at the recommended rate and from 2.3 to 3.9 days at the doubled rate of the recommendation. Initial residue amounts of bifenthrin and lambda-cyhalothrin at the recommended rate and doubled rate of recommendation and of deltamethrin at the recommended rate were less than their MRL, whereas, in case of deltamethrin sprayed at doubled rate of the recommendation, the residue level exceeded its MRL. However, the residue levels of the pesticides in the crop sampled at harvest were less than their maximum residue levels and the ratios of the estimated daily intake (EDI) to acceptable daily intake (ADI) for the pesticides tested calculated from the residue data at harvest were less than 7%, suggesting that these pesticides would be safe for Korean cabbage from the residue concern.