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mtDNA Diversity and Phylogenetic Analysis of Korean Native Goats (한국재래염소의 mtDNA 다양성 및 계통유전학적 분석)

  • Kim, Jae-Hwan;Cho, Chang-Yeon;Choi, Seong-Bok;Cho, Young-Moo;Yeon, Seung-Hum;Yang, Boh-Suk
    • Journal of Life Science
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    • v.21 no.9
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    • pp.1329-1335
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    • 2011
  • Korean native goats, which are characterized by black coat color, have existed on the Korean peninsula for a long time. Until now, there has been no comprehensive investigation concerning their genetic diversity, phylogenetic analysis or origin. In this study, we investigated the genetic diversity and verified phylogenetic status of the Korean native goat using the 453-bp fragment of the hypervariable fragment I (HVI) of mitochondrial DNA (mtDNA) D-loop region from 60 individuals among 5 populations. The Korean native goat showed less haplotype diversity when compared with goats from other countries. In addition, 6 haplotypes that had not been previously reported were verified in this study. In phylogenetic analyses with other country's goats, 10 haplotypes from Korean native goats were classified into mtDNA lineage A. Moreover, in a phylogenetic tree for goats which contained mtDNA lineage A, 8 of 10 haplotypes could be included in a subgroup with goats from Vietnam and an area of China. However, none of the remaining haplotypes belonged to a major group of Korean native goats and were located on different independent positions. These results suggest that almost Korean native goats aligned more closely to China and Vietnam breeds in mtDNA lineage A and there was no gene flow from other mtDNA lineages. Our results will contribute to conservation strategies and genetic breeding of Korean native goats.

Comparison of meridians electric response property for laser and acupuncture stimulation (레이저자극과 수기자극에 대한 경락전기반응 특성비교)

  • Lee, Yong-Heum;Ryu, Yeon-Hang;Jung, Byoung-Jo;Shin, Tae-Min
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.11 no.12
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    • pp.2335-2342
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    • 2007
  • Laser stimulation has been widely studied and used in clinic. However, electrical response by laser stimulation on meridians has not been investigated. In this study, we compared electric potential of laser and acupuncture stimulation on meridians. We measured electric potential variation at acupoints(Samgan(LI3) and Hapgok(LI4)) on Large Intestine Meridian. In laser stimulation results, average peak electric potential is very low($7.53{\pm}3.44{\mu}V$) for before and after stimulation. However, acupuncture stimulation was performed in ground connection condition and resulted in huge variation of average peak electric potential($2.65{\pm}1.53mV$). That is, the intensity and pattern of electric potential were dependent on the ground connection condition and individual. Also, the electric potential pattern was very similar to the pattern of electric charge and discharge of capacitor. The acupuncture stimulation using a insulating needle resulted in lower average peak electric potential variation($0.25{\pm}0.16mV$) than that of acupuncture stimulation. It might present little electrical response of acupuncture stimulation using insulating needles. In point of electrical response, the laser stimulation was determined to be no acupuncture effect at meridian. Acupuncture stimulation seems to be most effective method to induce electrical response at meridians. The procedure and effect of acupuncture might be considered as energy consensus phenomenon by transportation of bio-ion charge between a practitioner and patient.

Design and Evaluation of Pulsed Electromagnetic Field Stimulation Parameter Variable System for Cell and Animal Models (세포 및 동물모델용 펄스형 전자기장 자극 파라미터 가변장치 설계 및 평가)

  • Lee, Jawoo;Park, Changsoon;Kim, Junyoung;Lee, Yongheum
    • Journal of Biomedical Engineering Research
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    • v.43 no.1
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    • pp.11-18
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    • 2022
  • An electromagnetic generator with variable stimulation parameters is required to conduct basic research on magnetic flux density and frequency for pulsed electromagnetic fields (PEMFs). In this study, we design an electromagnetic generator that can conduct basic research by providing parameters optimized for cell and animal experimental conditions through adjustable stimulation parameters. The magnetic core was selected as a solenoid capable of uniform and stable electromagnetic stimulation. The solenoid was designed in consideration of the experimental mouse and cell culture dish insertion. A voltage and current adjustable power supply for variable magnetic flux density was designed. The system was designed to be adjustable in frequency and pulse width and to enable 3-channel output. The reliability of the system and solenoid was evaluated through magnetic flux density, frequency, and pulse width measurements. The measured magnetic flux density was expressed as an image and qualitatively observed. Based on the acquired image, the stimulation area according to the magnetic flux density decrease rate was extracted. The PEMF frequency and pulse width error rates were presented as mean ± SD, and were confirmed to be 0.0928 ± 0.0934% and 0.529 ± 0.527%, respectively. The magnetic flux density decreased as the distance from the center of the solenoid increased, and decreased sharply from 60 mm or more. The length of the magnetic stimulation area according to the degree of magnetic flux density decrease was obtained through the magnetic flux density image. A PEMF generator and stimulation parameter control system suitable for cell and animal models were designed, and system reliability was evaluated.

Impact of the coastal structures on the water circulation near Gusipo coast, Yellow Sea, Korea (서해 구시포 해안에서 해수유동에 미치는 구조물의 영향)

  • Kim, Cha-Kyum;Park, Il Heum
    • Journal of Korea Water Resources Association
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    • v.55 no.11
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    • pp.865-875
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    • 2022
  • Field measurements and numerical simulations using EFDC model were performed to quantify the changes of water circulation near Gusipo coast located in the Yellow Sea of Korea to estimate the impact of the construction of the coastal structures (jetty, groin, Gusipo port and bridge). The model predicted tide and tidal currents agreed reasonably well with the measurements. The maximum currents during spring tide near the Gusipo Beach (GB) have the range of 20~40 cm/sec whereas those off the GB range from 60 to 80 cm/sec. The typical patterns of tidal current show parallel with the local isobath. Tidal currents flow northeastward during the flood tide whereas the currents during the ebb tide flow southwestward. The current speeds at shielded waters after the construction of coastal structures strongly decreased as compared with those before the construction. The tidal volume due to the construction of coastal structures was estimated using the depth averaged velocity for 24 hours of spring tide. Tidal volume after construction of coastal structures was compared with initial state (before construction). Tidal volume at present state (after construction of jetty, groin, Gusipo port and bridge) decreased by 28.4% as compared with that of the initial state. The volume after construction of jetty and groin decreased by 21.3%, and the volume after construction of Gusipo port and bridge decreased by 9.8%.

A Study on the Cleanliness Evaluation Methods for the Selection of Alternative Cleaning Agents (대체 세정제의 선정을 위한 세정성 평가방법 연구)

  • Shin, Jin-Ho;Lee, Jae-Hoon;Bae, Jae-Heum;Lee, Min-Jae;Hwang, In-Gook
    • Clean Technology
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    • v.15 no.2
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    • pp.81-90
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    • 2009
  • In this study various cleaning evaluation methods were tested and comparatively evaluated to help cleaning industry. In order to select alternative cleaning agents objectively and systematically, various cleaning evaluation methods such as gravimetric, optically simulated electron emission (OSEE), contact angle, and analytical instrument methods were employed for cleaning contaminants such as flux, solder and grease. The analytical instruments used in this work were Fourier transform infrared spectroscopy (FTIR), ultraviolet visible spectroscopy (UV-VIS) and high performance liquid chromatography (HPLC). The gravimetric method was able to measure cleaning efficiencies easily and simply, but it was not easy to analyze them precisely because of its limitation in the gravimetric measurement. However, the OSEE technique was able to measure quickly and precisely the clean ability of cleaning agents in comparison with the gravimetric method. The contact angle method was found to be necessary for taking special precaution in its application to the cleaning evaluation due to possible formation of tiny organic film on the substrate surface which might be generated from contaminants and cleaning agents. In case of precision analysis that cannot be done by gravimetric method, fine analytical instruments such as UV-VIS, FTIR and HPLC could be used in analyzing trace amount of flux, solder and grease quantitatively, which were extracted from the surface by special solvents.

Validation of Launch Vibration Isolation Performance of the Passive Vibration Isolator for the Scientific Payload BioCabinet for CAS500-3 (차세대중형위성 3호 과학탑재체 바이오캐비넷용 수동형 진동절연기의 발사진동 저감성능 검증)

  • Dong-Jae Seo;Yeon-Hyeok Park;Young-Jin Lee;Ji-Seung Lee;Kyung-Hee Kim;Soon-Hee Kim;Chan-Hum Park;Hyun-Ung Oh
    • Journal of Aerospace System Engineering
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    • v.18 no.4
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    • pp.81-88
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    • 2024
  • The payload BioCabinet of CAS500-3 is designed for 3D stem cell differentiation, culture, and analysis utilizing bio 3D printing techniques in space. The 3D printing technique was initially developed for orbital use; however, it lacks separate validation for extreme launch vibration environments, necessitating a design that mitigates the launch load on the payload. This paper proposes a passive vibration isolator with a low-stiffness elastic support structure and high damping characteristics to reduce the launch loads affecting the BioCabinet. We explore the high-damping characteristics through the superelastic effects of SMA (Shape Memory Alloys) and a multi-layered structure incorporating viscoelastic tape. The effectiveness of the proposed vibration isolation system was confirmed via launch vibration tests on a qualification model.

A Study on the Improvement of Image-Based Water Level Detection Algorithm Using the Region growing (Region growing 기법을 적용한 영상기반 수위감지 알고리즘 개선에 대한 연구)

  • Kim, Okju;Lee, Junwoo;Park, Jinyi;Cho, Myeongheum
    • Korean Journal of Remote Sensing
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    • v.36 no.5_4
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    • pp.1245-1254
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    • 2020
  • In this study, the limitations of the existing water level detection algorithm using CCTV images were recognized and the water level detection algorithm was improved by applying the Region growing technique. It applied three techniques (Horizontal projection profile, Texture analysis, and Optical flow) to estimate the water area, and the results were analyzed in a comprehensive analysis to select the initial water area. The water level was then continuously detected by the Region growing technique, referring to the initial water area. As a result, it was possible to confirm that the exact level of water was detected without being affected by environmental factors compared to the existing level detection algorithm, which had frequent mis-detection phenomena depending on the surrounding environmental factors. In addition, the water level was detected in the video showing flooded roads in urban areas, not in the video of the river. These results are believed to be able to supplement the difficulty of monitoring at all times with limited manpower by automatically detecting the level of water through numerous CCTV footage installed throughout the country, and to contribute to laying the foundation for preventing disasters caused by torrential rains and typhoons in advance.

Evaluation of Acute Toxicity of Plant Extracts, Lavender, Lemon Eucalyptus and Cassia Essential Oil (식물 추출물 라벤더, 레몬 유칼립투스 및 계피 오일의 급성독성평가)

  • Jeong, Mi-Hye;Kwon, Mi-Jeong;Park, Soo-Jin;Hong, Soon-Sung;Park, Kyung-Hun;Park, Jae-Eup;Yeon, Sung-Hum
    • The Korean Journal of Pesticide Science
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    • v.14 no.4
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    • pp.339-346
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    • 2010
  • The acute toxicity test of high bio-active plant essential oils was conducted with Lavender, Lemon eucalyptus and Cassia oils selected to develop environment-friendly insecticides. The results of acute oral toxicity using rats showed that $LD_{50}$ of over 2,000 mg/kg bw for Lavender, Lemon eucalyptus and Cassia oils. The calculated acute dermal $LD_{50}$ value of all testing materials was over 4,000 mg/kg bw. The Skin irritation test indicated that Lavender and Lemon eucalyptus oil have no irritation while Cassia oil has a moderate irritation. For the Eye Irritation test, the result showed no irritation for Lavender and Lemon Eucalyptus oil and irritation for Cassia oils. However, the irritation was not showed for Eye Irritationwashing test of Cassia oil. Consequently, the Lavender and Lemon eucalyptus oils were showed to be low in toxicity whereas Cassia oil indicated to cause a moderate irritation on the skin and eyes.

A Study on User's Mental Model for Services and User Interface of Interactive TV -Focusing on a Comparison DCATV and IPTV (인터랙티브 의 서비스 및 인터페이스에 TV 대한 사용자의 멘탈모델에 관한 연구 -DCATV(Digital Cable TV)와 IPTV(Internet Protocol TV) 비교를 중심으로)

  • Yeoun, Myeong-Heum;Ryu, Su-Min;Han, Ah-Reum;Cheon, Jeong-Eun
    • 한국HCI학회:학술대회논문집
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    • 2009.02a
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    • pp.1038-1044
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    • 2009
  • Interests for User Interface directly influencing on usability are also growing as the competition between DCATV and IPTV come to the surface, according to getting activated Interactive TV market. Therefore, this study has the purpose to understand mental models of users for Interactive TV service & Interface. The methods for this study are as follows. First, as the step considering literature study, we compared differences between DCATV and IPTV and caught up the concepts of DCATV and IPTV among some types of Interactive TV. Second, we examinated and analysed Hello TV, C&M, Broad&TV, MegaTV, myLGtv as the step of analysis for examples for Interactive TV. Third, as the step of researches observing users we analysed usability problems caused by differences of mental models between DCATV and IPTV. As a result, we could find there are some differences for mental model of users in Interface & services between DCATV and IPTV Especially, it was found that users feel confused very much regarding perception of real time broadcasting and VOD. We regards this findings can be used as basic for concept makings of Interactive TV, when TV interface is developed in the future.

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Exercise Radionuclide Ventriculography in Patients with Mitral Stenosis (승모판 협착증 환자에서 운동부하 심장풀스캔을 이용한 좌심실기능의 평가)

  • Lee, Do-Yun;Shim, Won-Heum;Kim, Han-Soo;Park, Seung-Jung;Cho, Seung-Yun;Kim, Sung-Soon;Lee, Woong-Ku;Kim, Myeong-Jin;Choe, Kyu-Ok;Park, Chang-Yun
    • The Korean Journal of Nuclear Medicine
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    • v.26 no.1
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    • pp.58-64
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    • 1992
  • We performed exercise radionuclide ventriculography to evaluate left ventricular (LV) function in 35 patients with pure mitral stenosis (MS). There were 6 males and 29 females ranging from 21 to 63 years of age (mean $37{\pm}10$ years). We also studied 8 healthy men as control group (mean age $27{\pm}5$ years). Each patients was evaluated at rest and during maximal exercise on an isokinetic bicycle ergometer. Peak filling rate (PFR), peak ejection rate (PER), ejection fracion (EF), end-diastolic volume (EDV), end-systolic volume (ESV), stroke volume (SV), and cardiac output (CO) were determined by the radionuclide technique. The results were summarized as follows: 1) LV systolic dysfunction and reduced PFR were noted in patients with MS. 2) EDV as well as SV decreased with exercise in patients with MS. 3) No significant increase in EF during exercise compared to rest value was observed because it was caused by reduced EDV and SV during exercise in patients with MS. 4) CO increased with exercise was significantly lower than normal in patients with MS. 5) Patients with MS were divided into two groups according to whether SF increased more than 5% druing exercise compared to resting state or decreased. Significant difference were found between these two groups. Patients with a fall in EF with exercise were older, had larger left atrial size, and had lesser decreased in ESV during exercise.

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