• Journal of Embryo Transfer
• /
• v.3 no.1
• /
• pp.48-52
• /
• 1988

우수정란의 이식전 성판별이 관한 연구를 수행하기 위하여 GTH와 PGF$_2$$\alpha$투여에 대한 난소반응과 회수난자의 발유단계별 동결융해후 생존성을 조사하였으며, 이식전 수정라느이 성판별을 위하여 H-Y항체 처리후 정상발육 난자의 염색체를 분석하여 다음과 같은 결과를 얻었다. 웅성 비장세포(male, spleen cells)를 면역원으로 mouse와 rat에 투여, 항혈청의 항체를 확인한 결과 mouse에서는 C57 BL계통과 rat에서는 DonRyu 계통이 항체생산능력이 우수하였다. 공란우 87두에 hormone(2500IU PMSG, 25mg PGF$_2$alpha)처리하여 평균 57.8%의 채란유과 두당 4.9개의 난자가 회수되었으며, 전체회수란자(427개)중 moula(162개)와 blastocyst(190개)의 정상발육란자는 82.4%였다. 동결융해후 회수된 난자 (312개)중, 형태적으로 정상인 난자(241개)의 비율은 77.2% 발육단계별 성적은 blastocyst(83.4%)가 morula(71.0%)보다 우수하였다. 항체와 보체(Guinea pig serum)로 처리된 82개의 morula중 15개(18.3%)가 blastocyst로 발육되어 이중 5개(33.3%)가 성이 판별되었으며, 모두 xx형 성염색체를 갖는 자성수정란으로 판명되었다.

• The Journal of the Korean bone and joint tumor society
• /
• v.12 no.2
• /
• pp.165-170
• /
• 2006

We reported a case of chondrosarcoma in proximal tibia in a 44-year-old man. MR images demonstrated a $3.5{\times}20$ cm sized bone tumor. In reconstruction of resected proximal tibia, we used the allograft bone and soft tissue defects were covered by medial gastrocnemius rotation flap and skin graft. There were no local recurrence and distant metastasis and any complication such as secondary infection, nonunion, metal failure at the time of the last follow-up. There was no limitation of knee motion through the appropriate rehabilitaion programs.

• Tuberculosis and Respiratory Diseases
• /
• v.50 no.6
• /
• pp.718-725
• /
• 2001

The implantation of malignant cells along the needle tract is an extremely rare complication after a percutaneous fine-needle aspiration biopsy(FNAB). However, it is very serious and may result in a change in the prognosis of lung cancer, especially in the curable early stage(T1-2,N0,M0). Recently, we experienced two cases of such complications. A 43 years old female underwent a fine needle aspiration biopsy and a right middle lobectomy with adjuvant chemotherapy due to an adenocarcinoma(T2N0M0). Two years later, a new tumor developed at the site of the needle aspiraton biopsy. It had the same pathological findings as the previous lung cancer. Therefore, it was concluded to be an implantation metastasis, and she was treated successfully by a right pneumonectomy and a resection of the chest wall mass with adjuvant radiotherapy. In another case, a 62 years old man was diagnosed with squamous cell lung cancer by a fine needle aspiration biopsy and underwent a right upper lobectomy(T2N0M0) with adjuvant chemotherapy. eight months later, a protruding chest wall mass developed at the aspiration site. It showed the same pathological findings as the previous lung cancer. Consequently, a total excision of the mass with adjuvant radiotherapy was done. Two years after the second operation, although the right lung was intact, a metachronous squamous cell lung cancer was found at the left lower lobe. The two patients were still alive 15 and 37months after thenresection of the chest wall mass, respectively.

• Journal of the Korean Arthroscopy Society
• /
• v.10 no.2
• /
• pp.203-208
• /
• 2006

Purpose: Previous transtibial double bundle posterior cruciate ligament (PCL) reconstruction methods have several problems in graft length and tibial fixation. We introduce new surgical method that is less restrictive by graft length and is more stable with single tibial fixation. Operative technique: After diagnostic arthroscopy, we prepare the graft, ream the tibial tunnel and perform the procedure for TransFix tibial fixation. Femoral 2 tunnel is made and graft is passed via anteromedial (AM) portal. Tibial fixation is done and femoral 2 graft is fixed sequentially at each knee position. Conclusion: TtransFix tibial single fixation method in double bundle PCL reconstruction provides more stable fixation, more free graft selection and prevents graft damage by passing the graft via AM portal.

• The Journal of the Korean bone and joint tumor society
• /
• v.9 no.2
• /
• pp.139-147
• /
• 2003

Purpose: The purpose of this study was to determine if bony union can be obtained when a bone graft substitute is transplanted in order to treat a benign bone tumor and if there is a difference between calcium sulfate and hydroxyapatite in the healing procedure when the degree of the bony union after the bone graft are compared. Materials and Methods: This study selected 20 cases, in which a curettage on the benign bone tumors was conducted and a bone graft substitute was transplanted. The area of the focus, the new bone formation, the recurrence of the focus in the plain radiographs and histological findings were observed. Results: Twenty cases (13 males, 7 females) were evaluated. Their mean age at surgery was 15.8 years (2~45), and the mean follow-up period was 3 years. The mean area of focus was 30.7 $cm^3$ in the radiographs, and 19 cases showed successful results in the ultimate visit, while 1 case has a recurrence of the focus. Conclusion: Calcium sulfate has osteoconduction and superior bioavailability, and is absorbed in vivo in proportion to the new bone formation. On the other hand, hydroxyapatite has good osteoconduction. It can result in better bone formation when it is combined with an autologous bone graft, autologous bone marrow, and an allogenic bone graft, but is absorbed in vivo more slowly than the former.

• The Journal of the Korean bone and joint tumor society
• /
• v.11 no.1
• /
• pp.62-70
• /
• 2005

Purpose: We compared the time how much time spent until the bone unions, when we grafted fresh frozen allograft during therapy of benign bone tumor depends on the degrees of bony unions. Materials and Methods: This study selected 29 cases, in which a curettage on the benign bone tumors was conducted and a fresh frozen allograft was transplanted. The area of the focus, the new bone formation, the recurrence of the focus and complications in the plain radiographs were observed. Results: The average time when we could find out bony unions in the radiographs is eleven weeks. The time when we could observe the unions in the radiographs are 11.4 weeks in allograft group, 10.7 weeks in allograft and autograft groups and 13.6 weeks in allograft and bone substitute. On radiologic findings, the average lesion size is 40 cm3. The time when we could find bony unions are 9.3 weeks in less than 40 $cm^3$ and 12.9 weeks in more than 40 $cm^3$. We could observe recurrences in two cases. Conclusion: The authors could get the similar results between fresh frozen allograft, allograft and autograft after curettage of benign bone tumor. We can think the ideal method which is the transplantation of autograft, but if we can’t get autograft enough, the best way which heal the defects is the transplantation of fresh frozen allograft.

• Journal of Embryo Transfer
• /
• v.11 no.3
• /
• pp.317-321
• /
• 1996

본 연구는 수정능획득유기물질로 알려진 카페인 헤파린을 병행처리하여 한우 정자의 첨체 반응율과 생존율을 알아보고 수정능획득과정 중에 단백질의 변화상을 전기영동방법으로 조사하였다. 동결융해후 정자의 생존율은 90%이상이었으나 전배양처리후 0.5시간에 70%로 감소하고 2시간 이후에는 35%로 감소하였다. 정자의 첨체반응율은 동결융해후 정상정자가 85.7%였으나 전배양시간에 따라 53.4%에서 14.3%로 감소하였다. 동결융해후 첨체가 소실된 생존정자는 9.4%였으나 전배양시간에 따라 17.2%에서 46.8%로 증가하였다. 원정액에서는 분자량 20,000정도에서 동결융해후 전자보다 특이적으로 많은 단백질량을 나타내었으나 동결융해 후 정자를 0.5에서 2.0시간으로 전배양하였을 때 전배양시간에 따른 단백질상의 변화는 대조구와 큰 차이가 없었다.

• Reproductive and Developmental Biology
• /
• v.32 no.3
• /
• pp.199-203
• /
• 2008

This study was performed to analyze the characterization of plasma hormonal levels during pregnancy in the Hanwoo recipients pregnant by artificial insemination (AI) or somatic cell nuclear transfer (SCNT) embryos. The synchronized recipients pregnant by SCNT embryos produced by Hanwoo fetal fibroblast cells (n=8) and by AI (control, n=5) were used. The plasma hormonal levels were measured by RIA (P4 and E2) and ELISA (cortisol), respectively. In control, the increase of E2 and the decrease of P4 were occurred immediately before the initiation of parturition. The expression pattern of plasma P4 was similar in both groups from 50 to 10 days before parturition, however, it did not decrease even at the expected date of labor in the SCNT recipients. The plasma cortisol was expressed a lower level during pregnancy in the SCNT recipients. But, the cortisol was increased in the cow aborted around 100 days of pregnancy (n=1). Based on these results, it can be postulated that the failure of the hormonal changes immediately before parturition in the SCNT recipients may be one of the most important reasons for a delayed parturition in clone calving.

• The Journal of the Korean dental association
• /
• v.35 no.12 s.343
• /
• pp.910-912
• /
• 1997

• 대한위암학회:학술대회논문집
• /
• 2001.11a
• /
• pp.54-54
• /
• 2001