• Title/Summary/Keyword: 응축부

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The Differentiation of the Cholinergic Nerve Cells at the Meynert Basal Nucleus of the Basal Forebrains in the Growth Period of Rat (흰쥐 전뇌 기저부 Meynert기저핵에서 출생 후 발육기간에 따른 콜린성 신경세포의 분화)

  • Hahm, Young-Ok;Kim, Soo-Jin
    • Applied Microscopy
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    • v.31 no.4
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    • pp.355-365
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    • 2001
  • This study was performed to investigate the distribution and differentiation on the immunoreacted cells of the ChAT (choline acetyltransferase) at the Meynert basal nucleus of the forebrains in the growth periods of rat, using the immunohistochemistric method. According to the cell shape and the ratio of long axis vs short axis of cell soma, the ChAT antibody reacted nerve cells in the Meynert basal nucleus of the rats were classified into six types. In the adult rats, the FD (frequency distributions) of round, oval and elongated cells were maximum. The FD of these types were shown to be progressively decreased during the postnatal development. In addition, the FD of elongated nerve cells in were observed in the adult rats respectively. This was thought to be the same phenomenon as those in the round and oval cells . The total mean volume of ChAT antibody reacted cell somata was lowest in the PND (postnatal days) 7 rats and was highest in the PND 21 rats. But, those were decreased to the adult. These results suggest that ChAT antibody reacted nerve cells grow up to PND 21 and then, differentiate into the various types by neurites outgrowing. On the electron micrography, the adult rat forebrain cells were obtained to be well developed ribosomes, polysomes , rough endoplasmic reticula and mitochondria. The immunreactivities were observed in ribosomes, polysomes, rough endoplasmic reticula and outer membrane of mitochondria. Golgi complexes were poorly developed and not showed jmmunreactivity. The ribosomes , polysomes and endoplasmic reticula are considered to be closely related to the inter cellular localization and biosynthesis of the ChAT but not Golgi complex. According to the results in the present study, it is considered that the ChAT-immunoreacted nerve cells in the Meynert basal nucleus of the rat forebrains are differentiated throughout the postnatal development with following processes of changes; 1) the cholinergic nerve cells develop postnatally 2) cell soma volumes gradually increase during the early postnatal days 3) and then, cells differentiate into the various types by projecting the neurites to the appropriate area after PND 21.

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Distribution and Differentiation of the Choline Acetyltransferase-immunoreactive Nerve Cells in the Basal Nucleus of Meynert of the Rat Forebrains During the Postnatal Development (흰쥐 출생후 발생에 따른 전뇌 기저부 Meynert기저핵에서 Choline Acetyltranseferase 면역반응 신경세포의 분포 및 분화)

  • Lee, Nam-Seob;Chung, Young-Wha
    • Applied Microscopy
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    • v.29 no.4
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    • pp.479-491
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    • 1999
  • This study was performed to investigate the distribution and differentiation of choline acetyltransferase (ChAT)-immunoreactive cells in the basal nucleus of Meynert of the postnatal and adult rat forebrains, utilizing techniques of immunocytochemistry. According to the cell shape and the ratio of long axis vs short axis of cell soma, the ChAT-immunoreactive nerve cells in the basal nucleus of Meynert of the adult rat were classified into six types. In the adult rat, the frequency distributions (FD) of round, oval, elongated, fusiform, triangular and polygonal cells were 9.4%, 35.5%, 32.1%, 5.9%, 9.1% and 8.0%, respectively. The FD of oval and round nerve cells on the postnatal day (PND) 14 were observed to be 18.7% and 51.5%, respectively. Those were shown to be progressively decreased during developmental process to the adult. Also, those of elongated and triangular nerve cells on the PND 21 were observed to be 30.4% and 10.1%, respectively. Those were shown to be same phenomenon a,1 those in the round and oval cells. Meanwhile, those of the triangular and polygonal nerve cells were progressively increased from the early postnatal stage to the adult. The total mean volumes of ChAT-immunoreactive cell somata in the PND 7 rat were the lowest $(1,083{\mu}m^3)$ and those in the PND 21 rat were shown to be the highest $(5,045{\mu}m^3)$. But in the adult, those were decreased to $(2,731{\mu}m^3)$. Those in the PND 21 rat were shown to be about 84.7% larger than those in the adult. On the electron micrography, the cell organelles such as ribosomes, polysomes, rough endoplasmic reticula (RER) and mitochondria were well developed in the PND 21 rat forebrains, but Golgi complexes were shown to be proliferating phase. Especially, ribosomes, polysomes and RER were immunoreactive in the tissues treated with 0.05% triton X-100. According to the observations in the present study, it is considered that the ChAT-immunoreactive nerve cells in the basal nucleus of Meynert of the rat forebrains are differentiated throughout the following processes of changes during the postnatal development: 1) increase of cell soma volumes with the differentiation of tell organelles and neurites, 2) increase in the FD of differentiated tell types and 3) cell schrinkage without cell loss. The ribosomes, polysomes and RER are considered to be closely related to the intracellular localization and biosynthesis of the ChAT but not Colgi complex.

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A Study on the Separation of Food-Waste Leachate into 3 Phases (음식물쓰레기 탈리액의 삼상분리)

  • Kim, Sangguk;Jeong, Minji;Kwon, Hyolee
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.11a
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    • pp.197-197
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    • 2010
  • 음식물쓰레기의 삼성분은 수분, 휘발분, 회분이며 이들이 차지하는 비율은 계절, 지역별로 다소 상이하지만 수분 약 80%, 회분3%, 휘발분 17%이다. 음식물쓰레기 전처리과정으로 이물질제거, 탈수공정이 있으며 탈수공정에서 다량의 탈리액이 발생한다. 본 연구에서는 탈리액을 데칸타를 이용하여 1차로 원심분리하여 고.액 분리한 액을 실험대상으로 하였다. 실험대상 탈리액의 물성은 BOD 78,800[mg/l], COD 41,000[mg/l], 부유물질 25,900[mg/l], 총질소 928[mg/l]이었다. 탈리액에는 기름성분(육류, 식용유등), 입자상물질등이 포함되어 있으며 이들은 난분해성 유기물질로, 이를 제거하는데 기존의 처리방법으로 많은 어려움이 있어 주요한 수질오염 발생원이 되고 있다. 예를들면 하수처리장 폭기조 수면에 유막을 형성하여 산소공급을 방해함으로 미생물번식을 방해하는 요인이 된다. 본 연구는 음식물쓰레기 탈리액의 수분, 고형분, 유분으로의 삼상분리에 관한 것이다. 유분은 에멀젼형태로 안정되게 수층에 분산되어 존재한다. 미세기포를 이용한 부상법의 경우 미세기포 표면과 유분의 화학적친화력이 낮아 기포표면에 유분이 잘 부착되지 않으며, 원심분리 방법만으로는 유분 분리효율이 낮고, 추출에 의한 분리시 추출액이 다량 소요되고 처리시간이 길며 추출액 비용이 많이 소요된다. 탈리액을 유분, 슬러지, 수분으로 분리하면 환경오염을 일으키는 주요성분을 신재생에너지 원료로 활용할 수 있다. 유분의 주성분이 동식물성 유지이므로 전처리시 산촉매를 이용 수분과 유리지방산을 제거하고 염기성촉매를 이용하여 전이에스테르화 반응을 거치면 바이오디젤인 FAME과 글리세롤으로 변환하므로 글리세롤을 분리하면 바이오디젤을 얻을 수 있다. 슬러지는 입자상 물질로 착화가 잘 되고 건조하면 발열량이 높으며 중금속등에 오염되지 않아 청정연료로 활용이 가능하다. 실험실에서의 탈리액 삼상분리방법은 다음과 같다. 탈리액 30ml당 추출액으로 노말헥산을 1ml를 가한 다음 플라스크에서 $80^{\circ}C$로 가열 후 방냉한다. 가열중 노말헥산의 손실을 방지하기 위하여 증발가스를 콘덴서에서 응축하여 플라스크로 재순환한다. 탈리액을 플라스크에서 꺼내어 원심분리기 rack에 300-400g씩 병에 각각 넣고 4,000rpm으로 30분간 운전한다. 탈리액은 상부로부터 유분층, 미세입자층, 수층, 슬러지층으로 분리된다. 각 층의 계면에서 2종의 성분이 약간 섞일 수 있다. 유분을 분리한 후 유분층 잔존물과 미세입자층, 수층 상층부의 혼합물을 취하여 50g씩 병에 넣고 3,500rpm으로 10분간 운전한 후 유분을 분리한다. 마지막으로 미세입자층만을 3,500rpm으로 10분간 원심분리한 후 유분을 따로 분리한다. 얻어진 유분은 rotary evaporator에서 $120^{\circ}C$로 가열하여 유분과 노말헥산을 분리하며 분리효율을 제고하기 위하여 감압하에서 운전한다. 분리된 유분의 고위발열량이 9,450[Kcal/kg]이었으며 원소분석 결과 탄소 74.7%, 수소 12.55%, 질소 0.08%, 유황분 0.0003%이었다. 분리된 유분의 양은 계절별로 시료별로 다르며 가을철에는 1.6-1.9%, 여름철은 1.0-1.3%이었다. 분리된 슬러지로부터 Hg, As, Cr, Cd, Pb 중금속 성분이 검출되지 않았으며 수분 2.8%, 휘발분 76.85%, 회분 7.52%, 고정탄소 12.83%이었고 원소분석결과 탄소 45.25%, 수소 7.46%, 질소 5.05%, 산소 34.39%, 유황분 0.33%이었으며 저위발열량은 4,480[Kcal/kg]이었다. 분리된 슬러지 양은 11-19% 이었다.

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Capacity Modulation of a Ground Source Multi-Heat Pump in the Part Load Condtions (지열원 멀티열펌프 시스템의 용량가변 특성 연구)

  • Cho, Chanyong;Oh, Myung Suk;Choi, Jong Min
    • 한국신재생에너지학회:학술대회논문집
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    • 2010.11a
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    • pp.120-120
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    • 2010
  • 에너지 절약적 차원에서 한 대의 실외기에 다수의 실내기가 접속되는 개별공조형 멀티 열펌프에 관한 연구 및 개발과 보급이 증대되고 있다. 본 연구에서는 복수의 실내기를 갖고 가변속 압축기를 채용한 지열원 물대공기 멀티열펌프시스템을 학교 현장에 설치하여 제조사의 운전 제어 알고리즘에 따라 일일 냉난방 실증 성능 특성을 분석하였다. 2008년 9월 30일의 냉방부하가 낮은 일자에 대하여 시간에 따른 부하 변동에 따라 압축기의 용량가변으로 실내기 냉방용량은 정격용량 대비 17.1%에서 111.3%의 범위에서 변이되었으나, 열펌프 유닛의 일일 최대 COP는 6.2를 나타냈으며, 일일 평균 열펌프 유닛 COP는 4.5로 열펌프 유닛 인증 기준 이상의 성능을 나타냈다. 2008년 11월 10일 대비 2008년 12월 15일에는 시스템 가동 중의 평균 외기온도가 $9.6^{\circ}C$ 감소하였으나, 각 재실공간의 용도별 부분 부하특성으로 11월 10일 보다 난방 부하량이 작게 나타났다. 하지만, 12월 15일에는 부하량이 매우 작아서 11월 10일보다 잦은 압축기 가변에 따른 손실로 일일 평균 COP가 낮게 나타났다. 2009년 1월 12일에는 정격 용량대비 94%의 부하율로 11월 10일과 12월 15일 대비 4배 이상의 부하량으로 압축기 용량 가변율이 작아서 난방용량과 COP 변화율이 작게 나타났다. 2009년 1월 12일에는 지중순환수의 실외열교환기 유입온도가 2008년 12월 15일보다 감소하고, 시스템의 난방용량 증가로 상대적으로 응축기와 증발기 크기가 감소한 효과로 히트펌프 유닛 COP가 감소하였으나, 본 지열원 열펌프 제조사에서 생산하고 있는 본 지열원 시스템과 동일한 압축기 등을 채용한 동일 용량의 공기열원 열펌프의 1월 12일 외기온도 $-10^{\circ}C$에서의 열펌프 유닛 COP 대비 70% 우수한 성능을 나타냈다. 본 지열원 히트펌프의 2008년 11월 10일의 일일 평균 히트펌프 유닛 COP는 외기온도가 낮고 일일 부하량이 크게 나타난 1월 12일의 일일 평균 히트펌프 유닛 COP 대비 37% 높게 나타났으나, 지중순환펌프의 정속운전으로 시스템 요구 지중순환수 유량 증가에 따른 성능 향상보다 소비전력 증가 영향이 커서 1월 12일의 시스템 COP 보다 33.3% 작은 값을 나타냈다. 본 연구의 용량 가변형 압축기를 채용한 지열원 멀티 히트펌프 유닛을 지열원 냉난방 시스템의 히트펌프 유닛으로 사용할 경우 시스템 최적화를 통한 시스템 COP 향상과 연간에너지 절감을 이루기 위해서는 히트펌프의 용량 변화 시에 지중순환펌프의 순환 유량 최적화를 위한 효율적 운전 제어 알고리즘 개발이 요구된다.

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A study on the burn-in test to accomplish high quality cockpit air of an ultra-sonic aircraft in the early stage of production (생산 초기 초음속 항공기 조종석의 고품질 공기 확보를 위한 burn-in test 연구)

  • Shin, Jae Hyuk;Park, Sung Jae;Seo, Dong Yeon;Jeong, Suheon
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.44 no.10
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    • pp.871-876
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    • 2016
  • Abnormal odor similar with burning smell often appears at the cockpit in the beginning of ultra-sonic aircraft without air filter due to the heating of production materials remained at the bleed air duct. Sources of the odor should be removed by burn-in test before test flight in order to prevent pilot confuses order with emergency such as fire of engine. However, the present method cannot prevent abnormal odor completely at the high altitude flight because maximum temperature of flight is higher than it of burn-in-test. This paper suggests burn-in test improved based on the analysis of thermal conditions of high altitude flight. It is verified that the existing burn-in test cannot cover thermal conditions of high altitude flight due to the discontinuous flow control, high change rate of temperature per unit time and difference between limit temperature of condenser and turbine. In order to overcome the limitations of current methods, the new burn-in test with continuous flow control are suggested. The continuous flow control are achieved by ram air inlet control. The effect of suggested method are verified by ground tests and flight tests. The results show the bleed air temperature can cover the temperature of high altitude flight and prevent abnormal odor at the flight test.

An Experimental Study on Flow Distributor Performance with Single-Train Passive Safety System of SMART-ITL (SMART-ITL 1 계열 피동안전계통을 이용한 유동분사기 성능에 대한 실험연구)

  • Ryu, Sung Uk;Bae, Hwang;Yang, Jin Hwa;Jeon, Byong Guk;Yun, Eun Koo;Kim, Jaemin;Bang, Yoon Gon;Kim, Myung Joon;Yi, Sung-Jae;Park, Hyun-Sik
    • Journal of Energy Engineering
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    • v.25 no.4
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    • pp.124-132
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    • 2016
  • In order to estimate the effect of flow distributors connected to an upper nozzle of CMT(Core Makeup Tank) on the thermal-hydraulic characteristics in the tank, a simplified 2 inch Small Break Loss of Coolant Accident(SBLOCA) was simulated by skipping the decay power and Passive Residual Heat Removal System(PRHRS) actuation. The CMT is a part of safety injection systems in the SMART (System Integrated Modular Advanced Reactor). Each test was performed with reliable boundary conditions. It means that the pressure distribution is provided with repeatable and reproducible behavior during SBLOCA simulations. The maximum flow rates were achieved at around 350 seconds after the initial opening of the isolation valve installed in CMT. After a short period of decreased flow rate, it attained a steady injection flow rate after about 1,250 seconds. This unstable injection period of the CMT coolant is due to the condensation of steam injected into the upper part of CMT. The steady injection flow rate was about 8.4% higher with B-type distributor than that with A-type distributor. The gravity injection during hot condition tests were in good agreement with that during cold condition tests except for the early stages.

Semi-rigid Elasto-Plastic Post Buckling Analysis of Space Frame by Using the Explicit Arc-Length Method (명시적 호장법을 이용한 공간프레임의 반강접 탄소성 후좌굴 해석)

  • Lee, Kyoung-Soo;Han, Sang-Eul
    • Journal of Korean Society of Steel Construction
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    • v.23 no.5
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    • pp.535-546
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    • 2011
  • In this paper, semi-rigid elasto-plastic post-buckling analysis of a space frame was performed using various explicit arc-length methods. Various explicit arc-length methodsand a large-deformation and small-strain elasto-plastic 3D space frame element with semi-rigid connections and plastic hinges were developed. This element can be appliedto both explicit and implicit numerical algorithms. In this study, the Dynamic Relaxation method was adopted in the predictor and corrector processesto formulate an explicit arc-length algorithm. The developed "explicit-predictor" or "explicit-corrector" were used in the elasto-plastic post-buckling analysis. The Eulerian equations for a beam-column with finite rotation, which considers the bowing effects, were adopted for the elastic system and extended to theinelastic system with a plastic hinge concept. The derived tangent stiffness matrix was asymmetrical due to the finite rotation. The joint connection elements were introduced for semi-rigidity using a static condensation technique. Semi-rigid elasto-plastic post-buckling analyses were carried out to demonstrate the potential of the developed explicit arc-length method and advanced space frame element in terms of accuracy and efficiency.

Spermatogenesis of Black Molly and Sailfin Molly (Poeciliidae, Teleostei) (경골어류 난태생 송사리과에 속하는 black molly와 sailfin molly의 정자형성과정)

  • Ryu, Seung-Jun;Kim, Dong-Heui
    • Applied Microscopy
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    • v.40 no.3
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    • pp.169-176
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    • 2010
  • Black molly (Poecilia sphenops) and sailfin molly (Poecilia latipinna) are a teleost belonging to Poeciliidae. The spermatogenesis between two species were investigated by light and electron microscope. The whitish testes of both black molly and sailfin molly were located between intestine and air bladder. The size of testis was major axis 7 mm, minor axis 2 mm. The testis contained numerous testicular cysts. In both black molly and sailfin, primary spermatocytes were comparatively large ellipsoidal, and mitochondria showed a marked development. The secondary spermatocyte was smaller than that of primary spermatocyte, highly condensed according to their development. The nucleus with electron-dense was round shape and flagella started to be formed. In spermiogenesis, chromatin was more condensed. The mitochondria were rearranged along the tail. The number of mitochondria was 2 to 4 in cross section and 8 to 10 in longitudinal section. The head of mature sperm was long cone shape and had not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the tail of sperm have lateral fins. In conclusion, spermatogenesis and sperm morphologies of these two species were same. These morphological similarity seems to be an indication of the Poeciliidae.

Pressure Distribution over Tube Surfaces of Tube Bundle Subjected to Two-Phase Cross-Flow (이상 유동에 놓인 관군의 표면에 작용하는 압력 분포)

  • Sim, Woo Gun
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.37 no.1
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    • pp.9-18
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    • 2013
  • Two-phase vapor-liquid flows exist in many shell and tube heat exchangers such as condensers, evaporators, and nuclear steam generators. To understand the fluid dynamic forces acting on a structure subjected to a two-phase flow, it is essential to obtain detailed information about the characteristics of a two-phase flow. The characteristics of a two-phase flow and the flow parameters were introduced, and then, an experiment was performed to evaluate the pressure loss in the tube bundles and the fluid-dynamic force acting on the cylinder owing to the pressure distribution. A two-phase flow was pre-mixed at the entrance of the test section, and the experiments were undertaken using a normal triangular array of cylinders subjected to a two-phase cross-flow. The pressure loss along the flow direction in the tube bundles was measured to calculate the two-phase friction multiplier, and the multiplier was compared with the analytical value. Furthermore, the circular distributions of the pressure on the cylinders were measured. Based on the distribution and the fundamental theory of two-phase flow, the effects of the void fraction and mass flux per unit area on the pressure coefficient and the drag coefficient were evaluated. The drag coefficient was calculated by integrating the measured pressure on the tube by a numerical method. It was found that for low mass fluxes, the measured two-phase friction multipliers agree well with the analytical results, and good agreement for the effect of the void fraction on the drag coefficients, as calculated by the measured pressure distributions, is shown qualitatively, as compared to the existing experimental results.

In vivo Growth Inhibition of Sarcoma-180 Cells by a β-Glucan from the Mushroom Ganoderma lucidum (영지(Ganoderma lucidum)의 β-Glucan에 의한 Sarcoma-180 육종암 생장 억제)

  • Han, Man-Deuk;Kim, Yong Hyun;Kim, Wan Jong
    • Journal of Life Science
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    • v.24 no.7
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    • pp.721-727
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    • 2014
  • Mushroom-derived ${\beta}$-glucan, a polysaccharide (GLP) isolated from the mycelium of Ganoderma lucidum, was previously shown to have inhibitory effects against tumor-bearing mice in vivo. We investigated the apoptotic effect of mushroom-derived ${\beta}$-glucan in a sarcoma-180 tumor cell- bearing mice model using an ELISA to determine the levels of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in the mice. The morphology of the tumor cells was assessed with transmission electron microscopy (TEM). GLP was injected into the tumor-bearing mice at a dose (i.p.) of 20 mg/kg for 10 days. After 30 days, the tumor mass from the inguinal region was collected, weighed, and assayed using TEM and a TNF-${\alpha}$ ELISA kit. The tumors that developed in the mice treated with GLP were 71.4% smaller than those in the control group, showing the ability of GLP to inhibit tumor growth. The levels of TNF-${\alpha}$ in the serum of the sarcoma-180 bearing mice were 12 times greater than in the serum of the nonbearing tumor mice. An ultrastructural study demonstrated that the GLP-treated sarcoma-180 tumor cells were condensed, with rearranged chromatin. In addition, the marginated chromatin in nucleus induced the nuclear compartment, and there were many vacuolization in the cell. GLP could be an effective apoptosis-inducing compound in sarcoma-type cancers.