• Title/Summary/Keyword: 음성공학

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Incidence of Plasmids in Marine Bacteria Isolated from the Bunker-C Oil Enriched Culture (Bunker-C유 집식배양으로부터 해양세균 Plasmid의 분포)

  • Park, In-Sick;Park, Jung-Youn;Jin, Deuk-Hee;Hong, Yong-Ki
    • Microbiology and Biotechnology Letters
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    • v.16 no.4
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    • pp.270-274
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    • 1988
  • Samples used for the enrichment culture were collected from the sea water of suspected chronic petroleum contamination in the vicinity of Pusan, Chungmu and Ulsan ports, Korea. Alkaline Iysis and agarose gel electrophoresis techniques were employed to screen these isolates for the presence of plasmid DNA. There were n little differences in the percentage of isolates containing plasmids between sampling sites of unpolluted sen water (22%) and polluted son water (25%). Bacterial isolates taken from the Bunker-C oil enriched culture showed significantly more plasmid incidence (29%). About two thirds of strains grown on a variety of hydrocarbons were Gram negative strains of which 33% contained one or more plasmids. Multiple plasmids were observed in 23% of the plasmid-carrying strains. Forty one percent of the plasmids detected were estimated to have a mass of 20 kb or more.

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Transfer and genetic recombination of antibiotic resistance genes occurring in water environment (수질환경에서 일어나는 항생물질 내성유전자의 전이와 재조합)

  • 김치경;이성기;김영창
    • Microbiology and Biotechnology Letters
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    • v.14 no.3
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    • pp.245-250
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    • 1986
  • Antibiotic resistant bacteria isolated from the river water in Cheongju City were studied on the transfer of their R-plasmids in water environment. The R-plasmids were transferred by conjugation between the isolates ai the frequencies of 1.1$\times$10$^{-6}$ to 1.2$\times$10$^{-7}$ under laboratory condition and 1.2$\times$10$^{-7}$ to 1.0$\times$10$^{-9}$ in natural environment. The R-plasmids isolated from those bacteria were also transferred into the recipient cells of E. coli HB101 at the frequencies of 1.7-6.7$\times$10$^{-6}$. The AP$^{r}$Cm$^{r}$Tc$^{r}$-plasmid of isolate T-44 which were transformation by conjugation and transformation was determined to be 9.01 kilobses in molecular size. When the AP$^{r}$Cm$^{r}$Tc$^{r}$-plasmid DNA was treated with restriction endonuclease, the plasmid appeared to have one restriction site for EroRI and BamHI, respectively, and three sites for Pst I endonuclease.

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Polysaccharide Production by a Gram Negative Facultatively Anaerobic Rod (그람음성 간균에 의한 다당류의 생산)

  • Yoo, Jin-Young;Koo, Young-Jo;Shin, Dong-Hwa;Chung, Dong-Hyo
    • Microbiology and Biotechnology Letters
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    • v.16 no.2
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    • pp.98-104
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    • 1988
  • A bacterial isolate FRI-33 which produces hydrophillic polysaccharide was identified and its cultural condition was investigated. FRI-33 was identified as Enterobacter agglomerans. The optimum cultural conditions for polysaccharide production were 3$0^{\circ}C$, pH 5.7, using medium composed of glucose 25 g/$\ell$, peptone 2.0 g/$\ell$, yeast extract 0.5 g/$\ell$, KH$_2$PO$_4$ 1.0g/$\ell$, MgSO$_4$.7H$_2$ O 1.0g/$\ell$, CaCO$_3$ 2.5g/$\ell$. The polysaccharide production after 72 hours was 8.41 g/$\ell$. The polysaccharide was composed of galactose (1.0 mole), xylose (1.5 mole), gluconodeltalactone (1.9 mole) and ribose (0.03 mole). The apparent viscosity of 1% polysaccharide solution was 504 mPa.s at 60 rpm and intrinsic viscosity was 45.80 d$\ell$/ g.

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Application of Bacteria Isolated from Dok-do for Improving Compressive Strength and Crack Remediation of Cement-sand Mortar (독도산 탄산칼슘형성세균에 의한 모르타르 균열보수와 압축강도 증진)

  • Park, Sung-Jin;Lee, Na-Young;Kim, Wha-Jung;Ghim, Sa-Youl
    • Microbiology and Biotechnology Letters
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    • v.38 no.2
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    • pp.216-221
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    • 2010
  • This study shows an application of microbiologically induced carbonate precipitate for strength improvement and crack remediation of cement-sand mortar. Seven calcium carbonate-forming bacteria (CFB) were isolated from Dok-do and partially identified by DNA sequence analysis of the 16s rRNA gene. Crystal aggregates were apparent around the bacterial colonies grown on an agar medium. These strains showed strain specific $CaCO_3$ precipitation on urea-$CaCl_2$ medium. Among 7 isolates, Arthrobacter nicotinovorans KNUC601, Microbacterium resistens KNUC602, Agrobacterium tumefaciens KNUC603, Exiguobacterium acetylicum KNUC604, and Bacillus thuringiensis KNUC606 showed a repairing of artificial forced cracks in cement-sand mortar. Compressive strength of cement-sand mortar consolidated with Stenotrophomonas maltophilia KNUC605 was increased around 14.07% compared with that of negative control.

Remote Fault Detection in Conveyor System Using Drone Based on Audio FFT Analysis (드론을 활용하고 음성 FFT분석에 기반을 둔 컨베이어 시스템의 원격 고장 검출)

  • Yeom, Dong-Joo;Lee, Bo-Hee
    • Journal of Convergence for Information Technology
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    • v.9 no.10
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    • pp.101-107
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    • 2019
  • This paper proposes a method for detecting faults in conveyor systems used for transportation of raw materials needed in the thermal power plant and cement industries. A small drone was designed in consideration of the difficulty in accessing the industrial site and the need to use it in wide industrial site. In order to apply the system to the embedded microprocessor, hardware and algorithms considering limited memory and execution time have been proposed. At this time, the failure determination method measures the peak frequency through the measurement, detects the continuity of the high frequency, and performs the failure diagnosis with the high frequency components of noise. The proposed system consists of experimental environment based on the data obtained from the actual thermal power plant, and it is confirmed that the proposed system is useful by conducting virtual environment experiments with the drone designed system. In the future, further research is needed to improve the drone's flight stability and to improve discrimination performance by using more intelligent methods of fault frequency.

Analysis of Soil Moisture Monitoring at a Hillslope in Forested Watershed (산림유역 사면에서의 토양수분 시계열 관측 분석)

  • Jang, Eunse;Gwak, Yong Seok;Lee, Jung Hun;Lee, Yeun Gil;Jung, Sung Won;Kim, Sang Hyun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2015.05a
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    • pp.490-490
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    • 2015
  • 산림 사면에서의 토양층 수문반응은 물순환을 이해하는데 중요하다. 본 연구에서는 토양수분자료를 이용하여 토양수분의 계절적, 공간적 변화를 분석하였다. 연구대상지역은 경기도 파주시 적성면 설마리의 설마천 유역 내에 위치한 범륜사사면과 충청북도 음성군의 청미천 유역내의 수레의산사면이다. 정밀측량을 통해 획득한 수치고도모형(Digital Elevation Model, DEM)을 이용하여 관측지점들을 각각 선정하였다. 대상사면에 토양층별 토양수분의 분포변화를 분석하기 위해 각 지점별 10, 30, 60cm에서 토양수분량 측정시스템(Time Domain Reflectometry, TDR)방식의 토양수분측정장비(miniTRASE)를 설치하여 2시간 간격으로 2014년 3월부터 12월까지 토양수분을 측정하였다. 각 지점별 사면에서 획득된 토양수분 시계열자료는 토양수분의 시공간적 분포특성을 파악하기 위해 지점별 토양수분량의 통계분석(평균, 표준편차, 변동계수)를 수행하였다. 설마천유역의 범륜사사면에서는 2014년도에 특히 강우량이 적어 토양수분의 평균과 표준편차의 월별 변화에서는 기저토양수분 값을 주로 유지하고 강우에 대한 변동성이 크지 않은 것으로 나타났고, 청미천유역의 수레의산 사면에서는 시간적 토양수분변화는 계절적 강우분포 패턴에 따라 반응이 나타났다. 청미천유역의 토양수분자료를 이용하여 토양수분의 계절적, 공간적 변화를 분석한 결과 지표근처 토양층(10, 30cm)와 저층(60cm)에서의 공간적 변화특성이 다르게 나타났다. 이는 저층(60cm)에서의 지표하 흐름, 기반암 존재의 영향차이에 의한 것으로 판단되고, 사면의 위치에 따라 토양수분의 안정화 정도가 다른 것을 확인할 수 있다.

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Farmer's Education for Water Conservation (농민 물절약 교육 실시)

  • Lee, Seul Gi;Choi, Kyung Sook;Do, Jong Won;Lee, Gwang Ya
    • Proceedings of the Korea Water Resources Association Conference
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    • 2018.05a
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    • pp.484-484
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    • 2018
  • 본 연구에서는 농림축산식품부 "농업 농촌부문 가뭄대응 종합대책(2015)"의 일환으로 가뭄에 의한 농촌지역 물부족 문제를 극복하기 위한 농민 물절약 교육을 실시하였다. 물절약 교육을 통해 가뭄상황에 적응할 수 있도록 하기 위해 물절약 교육 시범대상지역은 농민농업가뭄지도 및 재해연보 등의 자료를 활용하여 가뭄 취약지역을 8개도별로 한국농어촌공사 관할지구로 선별하여 선정하였다. 최종적으로 농민 물절약 시범교육이 실시된 지역은 경기 평택 석정지구, 강원 철원 토교지구, 충북 음성 용계지구, 충남 서산 신창지구, 경북 문경 경천지구, 경남 거창 가북지구, 전북 고창 신림지구, 전남 영암 월악지구가 선정되었다. 시범교육실시 앞서 대상지구의 용 배수로 현황, 수로노후화, 농민 물이용 실태 등 물손실 요인을 파악하였다. 시범교육은 기존과는 차별된 체험식 비주얼 물절약 교육이 실시되었으며, 시범대상지구별로 3단계 교육이 실시되었다. 1단계 교육은 이해단계로 플래쉬 동영상과 물이용 모의를 체험하게 하여 물 손실 원인 및 물 절약 방안을 모색하도록 하였다. 2단계 교육은 적용단계로서 물절약 시뮬레이터를 이용하여 평상시 농민의 물이용 관행에 따른 물손실 야기에 대한 이해와 가뭄시나리오에 따른 물 공급량 감소 시 농민 피해 및 가뭄극복을 위한 효율적인 물이용에 대한 효과를 이해시키고자 하였다. 또한 이를 통해 농민이 스스로 물절약을 위한 행동강령을 도출하도록 하였다. 마지막 3단계는 실천단계로 실제 현장여건에서의 물절약 실천을 도모하기 위해서, 현장에서의 물손실 상황 파악 및 현장여건에 맞는 물절약 실천방안을 도출하도록 하였다. 교육을 통해 농민의 물절약 필요성에 대한 공감대를 형성하고, 물절약을 스스로 실천할 수 있도록 유도 하고자 하였다. 물절약 교육의 효과를 파악하기 위해서 설문조사를 실시하였으며, 그 결과 농민들은 물절약의 필요성을 매우 높게 공감하며, 필지단위 물손실을 감소시키는 물꼬관리의 중요성을 인정하고, 농민 수로관리 참여에 대한 인식을 높이는 계기가 되었다.

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Analysis of IT Technology through the Trends in Home Video Game Console (가정용 게임기 동향을 통해 본 IT 기술 분석)

  • Bae, Jung-Min;Bae, Yu-Mi;Jung, Sung-Jae;Jang, Rea-Young;Sung, Kyung
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2014.05a
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    • pp.675-678
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    • 2014
  • One time, Home video game console's penetration was as comparable to the personal computer's penetration, growth has slowed since the advent of smartphones, tablets and moblie devices. But game console actively introducing new IT technologies not available in the pc games and mobile games, still keeping a firm position in the relevent market. In this paper Home video game console's history, contemporary trends, and learn about trends in the company, New IT technologies applied to gaming was analyzed. Home video console market become the arena of New IT technologies according to the introduction of New IT technologies such as gesture recognition technology, speech recognition technology, media facade technology, virtual reality technology.

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Optimal Algorithm and Number of Neurons in Deep Learning (딥러닝 학습에서 최적의 알고리즘과 뉴론수 탐색)

  • Jang, Ha-Young;You, Eun-Kyung;Kim, Hyeock-Jin
    • Journal of Digital Convergence
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    • v.20 no.4
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    • pp.389-396
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    • 2022
  • Deep Learning is based on a perceptron, and is currently being used in various fields such as image recognition, voice recognition, object detection, and drug development. Accordingly, a variety of learning algorithms have been proposed, and the number of neurons constituting a neural network varies greatly among researchers. This study analyzed the learning characteristics according to the number of neurons of the currently used SGD, momentum methods, AdaGrad, RMSProp, and Adam methods. To this end, a neural network was constructed with one input layer, three hidden layers, and one output layer. ReLU was applied to the activation function, cross entropy error (CEE) was applied to the loss function, and MNIST was used for the experimental dataset. As a result, it was concluded that the number of neurons 100-300, the algorithm Adam, and the number of learning (iteraction) 200 would be the most efficient in deep learning learning. This study will provide implications for the algorithm to be developed and the reference value of the number of neurons given new learning data in the future.

An analysis study on the quality of article to improve the performance of hate comments discrimination (악성댓글 판별의 성능 향상을 위한 품사 자질에 대한 분석 연구)

  • Kim, Hyoung Ju;Min, Moon Jong;Kim, Pan Koo
    • Smart Media Journal
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    • v.10 no.4
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    • pp.71-79
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    • 2021
  • One of the social aspects that changes as the use of the Internet becomes widespread is communication in online space. In the past, only one-on-one conversations were possible remotely, except when they were physically in the same space, but nowadays, technology has been developed to enable communication with a large number of people remotely through bulletin boards, communities, and social network services. Due to the development of such information and communication networks, life becomes more convenient, and at the same time, the damage caused by rapid information exchange is also constantly increasing. Recently, cyber crimes such as sending sexual messages or personal attacks to certain people with recognition on the Internet, such as not only entertainers but also influencers, have occurred, and some of those exposed to these cybercrime have committed suicide. In this paper, in order to reduce the damage caused by malicious comments, research a method for improving the performance of discriminate malicious comments through feature extraction based on parts-of-speech.