• Title/Summary/Keyword: 윤석환

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The Enzymatic Pattern of Bifdobacterium sp. Int-57 Isolated from Korean Feces (한국인 분변으로부터 분리한 Bifidobacterium sp. Int-57의 효소 Pattern)

  • 박헌국;강동현;이계호;윤석환;이세경;지근억
    • Microbiology and Biotechnology Letters
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    • v.20 no.6
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    • pp.647-654
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    • 1992
  • In order to study the physiological properties of the intestinal bacteria, we isolated the intestinal bacteria of Koreans and tested the enzymatic patterns. Isolated Bifidobacterium sp. Int-57 had the higher activity of $\alpha$-glucosidase, $\beta$-glucosidase, $\alpha$-galactosidase, $\beta$-galactosidase. $\beta$-xylosidase and $\alpha$-arabinofuranosidase than other intestinal microorganisms. The effect of the carbon sources on the production of each enzymes of Bijidobacterium sp. Int-57 was investigated. The most suitable carbon source for the production of $\beta$-glucosidase was maltose, for a-glucosidase cellobiose, for $\alpha$-galactosidase raffinose, for $\beta$-galactosidase lactose, and for $\beta$-xylosidase and $\alpha$-arabinofuranosidase xylose, respectively. In addition, we investigated the optimal conditions and pH stability of each crude enzymes. The optimal condition of a-glucosidase was pH 6.0 and $40^{\circ}C$. that of Jj-glucosidase pH 7.0 and 50oe, that of $\beta$-galactosidase pH 7.0 and $50^{\circ}C$, that of $\beta$-xylosidase pH 6.0 and $40^{\circ}C$ , and that of $\alpha$-arabinofuranosidase pH 5.0 and $50^{\circ}C$. respectively. a-Glucosidase was stable at pH 4.0-9.0. Jj-glucosidase at pH 4.0-7.0. $\beta$-galactosidase at pH 4.0-9.0, $\beta$-xylosidase at pH 4.0-6.0, and /3-arabinofuranosidase at pH 7.0-9.0, respectively.

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Evaluation of metabolic tumor volume using different image reconstruction on 18F-FDG PET/CT fusion image (18F-FDG PET/CT 융합영상에서 영상 재구성 차이에 의한 MTV (Metabolic tumor volume) 평가)

  • Yoon, Seok Hwan
    • Journal of the Korea Convergence Society
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    • v.9 no.1
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    • pp.433-440
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    • 2018
  • Recently, MTV(metabolic tumor volume) has been used as indices of the whole tumor FDG uptake on FDG PET image but it is influenced by image reconstruction. The purpose of this study was to evaluate the correlation between actual volume and metabolic tumor volume applying different SUVmax threshold for different reconstruction algorithm on phantom study. Measurement were performed on a Siemens Biograph mCT40 using a NEMA IEC body phantom containing different size six spheres filled with F18-FDG applying four SBRs (4:1, 8:1, 10:1, 20:1). Images reconstructed four algorithms (OSEM3D, OSEM3D+PSF, OSEM3D +TOF, OSEM3D+TOF+PSF) and MTV were measured with different SUVmax threshold. Overall, the use of increasing thresholds result in decreasing MTV. and increasing the signal to background ratio decreased MTV by applying same SUVmax threshold. The 40% SUVmax threshold gave the best concordance between measured and actual volume in PSF and PSF+TOF reconstruction image. and the 45% threshold had the best correlation between the volume measured and actual volume in OSEM3D and TOF reconstruction image. we believe that this study will be used when the measurement of MTV applying various reconstruction image.

Radiation Exposure According to Radiation Technologist' Working Departments (방사선 종사자 근무 분야별 피폭에 관한 검토)

  • Yoon, Chul-Ho;Yoon, Seok-Hwan;Choi, Jun-Gu
    • Journal of radiological science and technology
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    • v.31 no.3
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    • pp.217-222
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    • 2008
  • Radiation dose to radiologists working at three hospitals in Seoul was investigated from Jan 1, 2006 to Dec. 31, 2006. The results are as follows. First, radiation dose to radiologists at a cardiac angiography room was measured as 1.41mSv, the highest while radiation dose to radiologists at a department of radiation oncology was measured as 0.64 mSv, the lowest. Second, radiation dose proves to be in direct proportion to the number of X-ray treatment. Third, as for the radiation dose in X-ray treatments, radiologists in cardiac angiography room are exposed to the largest amount of radiation while radiologists in diagnostic radiology department are exposed to the smallest amount of radiation. Last, radiation dose at a cardiac angiography room is the largest and is followed by nuclear medicine, diagnostic radiology, and radiation oncology departments in order. According to ICRP, exposure less than 20mSv per year is highly recommended while radiation dose is allowed as long as it is ranged less than 50mSv per year or 100mSv within a 5-year period. Taking into account the results, radiation exposure does not do any harm to radiologists at any related departments in Korean hospitals because the dose per year is less than 1.60mSv.

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Ecological Characteristics and Long-term Variation of Fish Community in Lake Paldang and its Tributaries (팔당호 어류군집의 생태특성 및 장기 변동)

  • Park, Hae-Kyung;Lee, Jangho;Choi, Myung-Jae;Yun, Seuk-Hwan;Song, Ho-Bok;Lee, Kyoo;Youn, Seok Jea;Shin, Kyungae;Byeon, Myeong-Seop;Kong, Dongsoo
    • Journal of Korean Society on Water Environment
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    • v.25 no.6
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    • pp.951-963
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    • 2009
  • The community structure and ecological characteristics of fish community in Lake Paldang were investigated from May to September 2008. During the survey period, 53 species belonging to 13 families were collected. Dominant species was Squalidus japonicus coreanus representing 81.8% of total number and 25.7% of total biomass of collected fish and subdominant species was Lepomis macrochirus representing 3.3% of total number and 18.9% of total biomass of collected fish. There were 20 Korean endemic species (38% of collected species number) including Cottus koreanus and 4 exotic species (7.5%) including Micropterus salmoides. The similarity analysis of fish communities among water areas of Lake Paldang using UPGMA showed that fish community of North-Han River was similar to that of South-Han River area and was different from that of Gyeongan River area. Long-term variation of fish community from 1972 to present study in Lake Paldang showed decrease of the species numbers after dam construction until 1994, and gradual increase from 1996 to present study indicating the disturbance of lentic system by dam construction in the 1980s. The increase of species number in the late 2000s may results in part from the increase of survey sites of successive studies. L. macrochirus which was designated as a domestic ecosystem-disturbing alien species with wide food niche have shown more than 20% of relative abundance since 1996 indicating the adverse effect on not only fish community but also aquatic ecosystem food web of Lake Paldang.

Stream Health Assessments on Tributaries of Lake Paldang Using Index of Biological Integrity for Fish Community and Physical Habitat Parameters (어류 모델 메트릭과 물리적 서식지 변수를 이용한 팔당호 유입하천 하류부의 하천건강성 평가)

  • Choi, Myung-Jae;Park, Hae-Kyung;Lee, Jang-Ho;Yun, Seok-Hwan
    • Korean Journal of Ecology and Environment
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    • v.42 no.3
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    • pp.280-289
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    • 2009
  • The fish communities and physical habitat conditions of fifteen tributaries of Lake Paldang in spring and autumn, 2008 were surveyed to evaluate the ecological health of the streams. The total 2,746 individuals were collected belonging to 11 families 31 genera 40 species. Two new species (Cottus koreanus, Gnathopogon strigaus) that have never been reported as yet in Lake Paldang watershed were found for the first time. The most dominant species in the tributaries was Acheilognathus yamatsutae (19.9%) which is Korean endemic species. Ecological health evaluation of fifteen tributaries using index of biological integrity (IBI) model for fish community and qualitative habitat evaluation index (QHEI) was performed. According th the IBI analysis, four streams (Siwoo-Stream, Jojong-Stream, Moonho-Stream and Mugab-Stream) were evaluated as "good" condition (B grade), Woosan-Stream were "poor" condition (D grade) and others were "fair" condition (C grade). Qualitative habitat evaluation index values of the four streams were the grade "II" indicating "good" condition and those of eleven streams were the grade "III", indicating 'fair' condition. On the whole, dataset of IBI and QHEI showed that ecological health of Jojong-Stream has been well maintained compared to other tributaries of Lake Paldang.

The Latest Trend of Dynamic MR Defecography for the Chronic Constipation Patient (만성 기능성 변비 환자에서 동적 MR Defecography의 최신동향)

  • Yoon, Seok-Hwan
    • Journal of radiological science and technology
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    • v.27 no.4
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    • pp.17-21
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    • 2004
  • With advancement of the medical imaging technology, the dynamic pelvic MRI (magnetic resonance imaging) has been introduced and used for dynamic MR defecography to improved diagnosis of the patients. At the early stage of its use, it was mostly used to diagnose enterocele or cystocele, then its use was extended to diagnose the organ prolapse and other dysfunctional pelvis organs. There now have been many reports of other diseases such as the functional constipation and others. This paper introduces the pelvis MRI and the dynamic MR defecography and reports the future trend in their clinical applications. Until recently, the studies with pelvic MRI were mostly focused on observing the movement of the pelvis in the supine position. Yang and 26 others reported good result in observing the patients with the pelvic organ prolapse by using the pubococcygeal line as the anatomical index. Using the fast gradient recalled acquisition (fast GRASS), they compared cystocoele, genitourinary prolapse, enterocoele and rectocoele with the control group. Kruyt et al. observed the posterior compartment and reported that MRI was more helpful than the fluoroscopy. Healy et al. applied the dynamic MRI test on the patients with constipation or incontinence as well as the control group without those symptoms. Since then, MRI technology has further advance by Lienemann, who was able to attain the more detailed images using the fast T2 weighted turbo spin echo technology, and others. If its limitation in diagnosing intussusception and the like, since the observation can be made only from the supine position, can be overcome with open MR or others, it is envisages that the method can eventually replace the radiological defecography.

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Optimization of solid phase extraction and simultaneous determination of trace anions in concentrated hydrofluoric acid by ion chromatography (불산 중 극미량 음이온 분석을 위한 고상 추출법 및 이온크로마토그래프를 이용한 동시분석법 확립)

  • Yoon, Suk-Hwan;Jo, Dong-ho;Kim, Hyun-Ji;Shin, Ho-Sang
    • Analytical Science and Technology
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    • v.29 no.5
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    • pp.219-224
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    • 2016
  • 불산 중 극미량 음이온의 고상추출과 이온크로마토그래프를 이용한 고감도 분석법이 개발되었다. 불산 중 불소이온이 고상에 의해 제거하였고 이어서 음이온 (F, CH3COO, Cl, Br, NO3, PO43−, SO42−)들이 이온크로마토그래프를 이용하여 연속적으로 분리하였다. 고상 추출법에 영향을 주는 각 인자들 (흡착제의 선택, 시료의 부피 및 pH, 용출 용액과 용출용액의 부피)을 결정하였으며 그 결과 흡착제로서 Oasis WAX 컬럼이 가장 우수하였고 1.0 mL의 시료부피, 용출용액으로 50 mM 초산암모늄염 5 mL가 분리능에서 가장 우수하였다. 개발한 방법에 의한 음이온 (Cl, Br, NO3, PO43−, SO42−)들의 방법검출한계는 25 % 불산용액 (w/w) 중에 0.04~0.30 µg/L의 범위를 보였고 정밀도는 20.0와 40.0 µg/L의 농도에서 5 % 이내를 보였다. 한 제조회사에 의한 25 % 불산 중 음이온의 4.2에서 47.5 µg/L의 범위로 모두 검출되었다. 이 방법은 시험절차가 간단하고, 재현성 및 감도가 좋아서 반도체회사에서 불산 중 음이온 불순물을 정도 관리하는데 매우 유용한 방법이 될 것으로 판단된다.

Development of Meat-like Flavor by Maillard Reaction of Model System with Amino acids and Sugars (Meat-like Flavor 개발을 위한 당-아미노산 Model System에서의 Maillard 반응)

  • Ko, Soon-Nam;Yoon, Suk-Hwan;Yoon, Suk-Kwon;Kim, Woo-Jung
    • Korean Journal of Food Science and Technology
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    • v.29 no.5
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    • pp.827-838
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    • 1997
  • Optimal substrates and reaction conditions were studied to develop boiled or roasted meat flavor by Maillard reaction under a model system. Sugars for the reactions were xylose, ribose, glucose, lactose, maltose, and sucrose. Amino acids were cystine, cysteine, methionine, lysine, and glycine as the reaction substrates. The reacted solutions were measured their absorbances at 278 nm and 420 nm and were evaluated their sensory properties. Except cysteine, the pentose mixtures with all of the four amino acids showed a faster reaction rate than those mixtures with hexose or disaccharides. pH was decreased rapidly until 8 hours and then changed a little thereafter. Sensory evaluation showed that cystine-lactose or cystine-xylose from single substrate and cystine-lactose-maltose, and cystine-lactose-xylose from mixed substrates reacted at $100^{\circ}C$ for 16 or 20 hours were found to be close to boiled or roasted meat flavor. The volatile compounds from the four selected sugar-amino acid solutions by GC/MS were 8 hydrocarbons, 10 aldehydes, 6 ketones, 7 alcohols, 2 aromatics (benzene), 1 ester, 4 furans, 1 base and 5 sulfur compounds.

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A Study on Changes of the Central Part of Beomeosa Temple - Based on Photographs and Drawings - (범어사(梵魚寺) 가람배치(伽藍配置)의 중단영역(中壇領域) 변화(變化)에 관한 연구(硏究) - 사진(寫眞)과 도판(圖版)을 중심(中心)으로 -)

  • Youn, Suk-Hwan;Han, Sam-Geon
    • Journal of architectural history
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    • v.14 no.4 s.44
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    • pp.41-58
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    • 2005
  • This study was started under the judgement that reviewing $\ulcorner$A Survey Report of Korean Architecture$\lrcorner$, which contains photographs and drawings by Sekino Tadashi and which was also made under the order of the Japanese government(1902), would make it possible to consider in detail the status of Korean buildings in the period of the Korean Empire. The focus of the study. was put on three main parts of the temple, especially the central parts. The photographs and drawings as mentioned above showed that changes in the layout of buildings in the central part of the temple were made between the period of the Korean Empire and that of Japanese occupation. Thus the purpose of the study is to investigate when changes in building layout in the central part of the temple were made and what made such changes to be caused, obtaining information necessary for future changes in the precincts of the temple. Results of the study can be summarized as follows. First, 'Seonchal Daebonsan Beomeosa feonkeongdo' and 'Gyeongsangnamdo Dongraegun Beomeosa Geonmul Jeondo' were made in a same period. While, 'Chosun Gojeok Dobo', published by the government-general of Chosun, was a collection of photographs taken by Sekino Tadashi for about 30 years from 1902 to 1933 under the order of the Japanese government. But there were few changes in the building layout of the temple shown in the three materials. Second, one of the photographs and drawings made since 1933, not contained in 'Chosun Gojeok Dobo', and discovered by this researcher was 'Seonchal Daebonsan Beomeosa Annae' which was published by the temple itself after national independence from the period of Japanese occupation, indicating the main reason of changes in building layout as mentioned above. In conclusion, the layout of buildings of the temple began to be changed on a large scale, with so-called 'Cheyongseol' ignored, when Buddhist monk Cha Woon Ho established a 7-storied sarira tower to the left of the main building in the center of the upper part in 1936. In addition, the axis line connecting between Hwaeomjong and Seonjong was also changed at that time.

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Performance Test of a 7 tonf Liquid Rocket Engine Turbopump (7톤급 액체로켓엔진용 터보펌프 조립체 성능시험)

  • Kwak, Hyun Duck;Kim, Dae-Jin;Kim, Jin-Sun;Kim, Jinhan;Noh, Jun-Gu;Park, Pyun-Goo;Bae, Jun-Hwan;Shin, Ju-Hyun;Yoon, Suck-Hwan;Lee, Hanggi;Jeon, Seong-Min;Jeong, Eunhwan;Choi, Chang-Ho;Hong, Soon-Sam;Kim, Seong-Lyong;Kim, Seung-Han;Han, Yeong-Min
    • Journal of the Korean Society of Propulsion Engineers
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    • v.19 no.2
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    • pp.65-72
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    • 2015
  • Performance tests of a turbopump for the developing 7-tonf liquid rocket engine were conducted. The performance of turbopump components and their power matching were measured and examined firstly under the LN2 and water environment. In the real propellant(LOX and kerosene) environment tests, design and off-design performances of turbopump were fully verified. During the off-design tests, turbopump running time was set the same as engine operating time and pump inlet pressure were set lower than nominal operating value in order to investigate pump suction capability. It have been verified that subject turbopump satisfies required performance - flow rate, head, suction performance and operational time - in the operating regime of developing liquid rocket engine.