• Korean Journal of Food Science and Technology
• /
• v.35 no.1
• /
• pp.15-22
• /
• 2003

Nutritional compositions of three Tochukaso species (Paecilomyces tenuipes hosted by Larva and pupa, Cordyceps militaris, C. sinensis) were compared. Fruiting body and host fractions were separately analyzed. Fruiting body fraction of P. tenuipes (36.6%) hosted by larva was higher than that hosted by pupa (10.2%), an indication that the quality of the former is superior to the latter. Carbohydrate content of C. sinensis (39.6%) was $2.5{\sim}7$ times higher than those of others, probably due to the presence of polysaccharides. Protein and crude lipid contents of C. sinensis and C. militaris were 25.8 and 10.3%, and 75.1 and 3.9%, respectively. C. sinensis showed the lowest Ca content and $30{\sim}75$ times higher Fe content among the samples tested. Vitamin A content of C. militaris was 308.9 IU/100g, two fold higher than those of the other species. Saturated fatty acid content was the highest in P. tenuipes (pupa, 27.7%), whereas unsaturated fatty acid was the highest in P. tenuipes (larva, 83.3%). Aspartic acid, glutamic acid, and glycine were abundant in all species. Cordycepin content of C. militaris was $20{\sim}50$ times higher than those of the other species.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.30 no.4
• /
• pp.684-695
• /
• 2003

The aim of this study was to investigate the effect of composite resin components on proliferation and glucan synthesis by cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus. Light curing pit and fissure sealant was chosen for evluation. Specimens were eluted in deionized water for 10 minutes, 1, 12, and 24 hours. Extracts of specimens were diluted into 1/2, 1/4, and 1/8 with addition of BHI broth and BHI-YS. Bacteria were cultured in media included eluted components, and measured optical density($A_{600}$). The following results were obtained 1. 1/4 concentration of elutes for 10 minutes significantly inhibited the proliferation of S. mutans, whereas 1/2, 1/8 concentration of elutes stimulated it. Also, exacts, especially 1/2, 1/4 concentration, for 1 hours stimulated it. But exacts for 12, 24 hours had not effects on the proliferation of S. mutans. 2. 1/4 concentration of elutes for 10 minutes inhibited growth of S. sobrinus, whereas extracts for 1, 12, 24 hours had not effects on the proliferation of S. sobrinuss. 3. Extracts from composite resin stimulated total growth of S. mutans more than growth control group, where as inhibited it of S. sobrinus. 4. Extracts from composite resin, especially 1/4 concentration of it for 10 minutes increased the formation of water insoluble glucan of S. mutans. But elutes for 1, 12, 24 hours, and 1/8 concentration of it for 10 minutes inhibited it. 5. Except 1/4 concentration of elutes for 10 minutes, extracts decreased the formation of water insoluble glucan of S. sobrinus. 6. Total amount of formated glucan was 3-fold higher in S. mutans than in S. sobrinus.

• Nuclear Medicine and Molecular Imaging
• /
• v.42 no.5
• /
• pp.383-393
• /
• 2008

Purpose: Cancer specific killing can be achieved by therapeutic gene activated by cancer specific promotor. Expression of sodium iodide symporter (NIS) gene causes transportation and concentration of iodide into the cell, therefore radioiodine treatment after NIS gene transfer to cancer cell could be a form of radionuclide gene therapy. luciferase (Luc) gene transfected cancer cell can be monitored by in vivo optical imaging after D-luciferin injection. Aims of the study are to make vector with both therapeutic NIS gene driven by AFP promoter and reporter Luc gene driven by CMV promoter, to perform hepatocellular carcinoma specific radiodiodine gene therapy by the vector, and assessment of the therapy effect by optical imaging using luciferase expression. Materials and Methods: A Vector with AFP promoter driven NIS gene and CMV promoter driven Luc gene (AFP-NIS-CMV-Luc) was constructed. Liver cancer cell (HepG2, Huh-7) and non liver cancer cell (HCT-15) were transfected with the vector using liposome. Expression of the NIS gene at mRNA level was elucidated by RT-PCR. Radioiodide uptake, perchlorate blockade, and washout tests were performed and bioluminescence also measured by luminometer in these cells. In vitro clonogenic assay with 1-131 was performed. In vivo nuclear imaging was obtained with gamma camera after 1-131 intraperitoneal injection. Results: A Vector with AFP-NIS-CMV-Luc was constructed and successfully transfected into HepG2, Huh-7 and HCT-15 cells. HepG2 and Huh-7 cells with AFP-NIS-CMV-Luc gene showed higher iodide uptake than non transfected cells and the higher iodide uptake was totally blocked by addition of perchlorate. HCT-15 cell did not showed any change of iodide uptake by the gene transfection. Transfected cells had higher light output than control cells. In vitro clonogenic assay, transfected HepG2 and Huh-7 cells showed lower colony count than non transfected HepG2 and Huh-7 cells, but transfected HCT-15 cell did not showed any difference than non transfected HCT-15 cell. Number of Huh-7 cells with AFP-NIS-CMV-Luc gene transfection was positively correlated with radioidine accumulation and luciferase activity. In vivo nuclear imaging with 1-131 was successful in AFP-NIS-CMV-Luc gene transfected Huh-7 cell xenograft on nude mouse. Conclusion: A Vector with AFP promoter driven NIS and CMV promoter driven Luc gene was constructed. Transfection of the vector showed liver cancer cell specific enhancement of 1-131 cytotoxicity by AFP promoter, and the effect of the radioiodine therapy can be successfully assessed by non-invasive luminescence measurement.

• Journal of the Korean Society of Hazard Mitigation
• /
• v.8 no.6
• /
• pp.149-153
• /
• 2008

The pollutant capacity occurred before and after the development of a watershed should be quantitatively estimated and controlled for the minimization of water contamination. The Ministry of Environment suggested a guideline for the legal management of nonpoint source from 2006. However, the rational method for the determination of treatment capacity from nonpoint source proposed in the guideline has the problem in the field application because it does not reflect the project based cases and overestimates the pollutant load to be reduced. So, we perform the standard rainfall analysis by analytical probabilistic method for the estimation of an additional pollutant load occurred by a project and suggest a methodology for the estimation of contaminant capacity instead of a simple rational method. The suggested methodology in this study could determine the reasonable capacity and efficiency of a treatment facility through the estimation of pollutant load from nonpoint source and from this we can manage the watershed appropriately. We applied a suggested methodology to the projects of housing land development and a dam construction in the watersheds. When we determine the treatment capacity by a rational method without consideration of the types of projects we should treat the 90% of pollutant capacity occurred by the development and to do so, about 30% of the total cost for the development should be invested for the treatment facility. This requires too big cost and is not realistic. If we use the suggested method the target pollutant capacity to be reduced will be 10 to 30% of the capacity occurred by the development and about 5 to 10% of the total cost can be used. The control of nonpoint source must be performed for the water resources management. However it is not possible to treat the 90% of pollutant load occurred by the development. The proper pollutant capacity from nonpoint source should be estimated and controlled based on various project types and in reality, this is very important for the watershed management. Therefore the results of this study might be more reasonable than the rational method proposed in the Ministry of Environment.

• Archives of design research
• /
• v.20 no.3 s.71
• /
• pp.279-288
• /
• 2007

Today, design is not free from fashion, which emerges and vanishes temporarily, and aims at equalization. As a result, products quickly become obsolete because of fashion. This means that the span of products is determined by a social concept, which is not clarified, regardless of their functions. Usable products will gradually disappear from us and it will cause serious environmental problems, unless we can find out measures against fashion. As such, it is important to study the characteristics of the shaker's design in this circumstance. The Shaker's community has a distinguishable difference from other general societies. Temporary fashion and misled information cannot interfere with their consciousness. Religion, the life and the principle of design have developed on the same level in their community. Especially, any decoration or the difference of materials is not allowed in shaker's design. It reflects their thinking that all people are equal in the sight of God. Therefore, any decoration for social and economical superiority can not be used. Through this consciousness, they can be free from fashion or decoration. They, also, believe that they can reach perfection through practicality and simplicity. The reason why shaker's design is not disturbed by fashion is that their belief is involved in their design. Consequently, if religious or conscious contents are primarily set up, design can be free from fashion and products can be used for a long time.

• Archives of design research
• /
• v.20 no.3 s.71
• /
• pp.299-314
• /
• 2007

For the past 20 years, the growth and development of university-design-educational institutes contributed to the industrial development of our country. Due to the technological fluctuation and changes in the industrial structure in the latter half of the 20th century, the enterprise is demanding professionally-oriented design manpower. The principle which appears from instances of the advanced nations is to accommodate the demands in social changes and apply them to educational design programs. In order to respond promptly to the industrial demand especially, the advanced nations adopted "multidisciplinary design education programs" to lead innovation in the area of design globally. The objective of the research consequently is to suggest an educational system and a program through which the designer can be educated to obtain complex knowledge and the technique demanded by the industry and enterprise. Nowadays in order to adapt to a new business environment, designers specially should have both the knowledge and techniques in engineering and business administration. We suggest that the IPDI, a multidisciplinary design educational system and program is made up of the coordinated operation of major classes, on-the-job training connection, educational system for research base creation, renovation design development program for the application and the synthesis of alternative proposals about the training facility joint ownership by connecting with the education of design, business administration and engineering.

• Archives of design research
• /
• v.20 no.3 s.71
• /
• pp.151-162
• /
• 2007

How can new media devices and networks provide an effective response to the world's growing sector of cultural and historically-minded travelers? This study emerged from the question of how mobile handsets can change the nature of cultural and historical tourism in ubiquitous city environments. As wireless network and mobile IT have rapidly developed, it becomes possible to deliver cultural and historical information on the site through mobile handset as a tour guidance system. The paper describes the development of a new type of mobile tourism platform for site-specific cultural and historical information. The central objective of the project was to organize this cultural and historical walking tour around the mobile handset and its unique advantages (i.e. portability, multi-media capacity, access to wireless internet, and location-awareness potential) and then integrate the tour with a historical story and role-playing game that would deepen the mobile user's interest in the sites being visited, and enhance his or her overall experience of the area. The project was based on twelve locations that were culturally and historically significant to Korean War era in Busan. After the mobile tour game prototype was developed for this route, it was evaluated at the 10th PIFF (Pusan International Film Festival). After use test, some new strategies for developing mobile "edutainment content" to deliver cultural historical contents of the location were discussed. Combining 'edutainment' with a cultural and historical mobile walking tour brings a new dimension to existing approaches of the tourism and mobile content industry.

• Korean journal of food and cookery science
• /
• v.11 no.2
• /
• pp.126-132
• /
• 1995

This study was undertaken to determine effect of parboiling on physical and cooking characteristic properties of milled rice. Equilibrium moisture content(EMC) of parboiled rice soaked at room tempe-rature(25$^{\circ}C$) and high temperature(75$^{\circ}C$) increased 1.8∼2.7 times & 1.4∼l.6 times as compare to raw rice, and time to reach EMC of parboiled rice became longer 4 times and 1.7 times than raw rice respectively. Equilibrium volume(EV), time to reach EV and volume increase rate constants(ku) were similar to EMC, time to reach EMC, and k. kv of parboiled rice brought about in decrease at room temperature and increase at high temperature. The geletinization temperature, time and peak viscosity of parboiled rice were higher than those of raw rice. Parboiling brought about in decrease in L/W of cooked parboiled rice kernels and L/W increased according to presoaking time was prolo-nged. Solid content of cooking water of raw rice were higher than those of parboiled rice. Hardness of uncooked PL20 & PL40 soaked for 30 min was higher than that of raw rice but that of uncookef parboiled rice soaked for 90 min was lower than that of raw rice. Springness of cooked parboile rice for initial 10 min decresed with that of cooked raw rice and then increased sharply, cooked f, r 15 min then increased slowly, cooked parboiled rice for 40 min increased more than cooked raw rice. Color differences($\Delta$I) of PT2O samples was the lowest L value of all the samples increased but a and b value decreased according to milling degree was high.

• Korean Journal of Soil Science and Fertilizer
• /
• v.32 no.2
• /
• pp.155-163
• /
• 1999

Nutrient balance during rice cultivation in the paddy of a local area under the environmental protection for drinking water supply was investigated. To compare nutrient balance in the paddy soil applied with different types of fertilization, 7 treatments were selected as followings : Recommended level of chemical fertilizers(R), Conventional fertilization(CF), Fresh cow manure(FCM), Cow manure compost(CMC), Straw compost+reduced chemical fertilizer(SCF), Fresh straw+recommended level of fertilizers(FSC), and no fertilization as control(C). Here, FCM, CMC and SCF were applied at the same level of total nitrogen as recommended in R. Rice yield was the highest in the recommendation(R) and fresh cow manure (FCM) treatments with $6,730kg\;ha^{-1}$(index 100), and followed by SCF (index 98), FSC (index 98), CMC(index 94), and CF(index 94). But statistically significant difference was not recognized among treatments except the control. Nitrogen infiltration loss was high in the simple chemical fertilizer treatments with $63kg\;ha^{-1}$ in CF and $58kg\;ha^{-1}$ in R during rice cultivation, respectively. Nitrogen infiltration loss was decreased below half level of chemical fertilizer treatments with cow manure treatments ($23kg\;ha^{-1}$ in FCM and $27kg\;ha^{-1}$ in CMC) and with reducing chemical fertilizer treatment by adding straw compost ($25kg\;ha^{-1}$). Phosphate was not leached during rice cultivation in paddy soil of a fluvial deposit type, in which oxidation horizon was developed broadly under around 15 cm depth of surface soil. Phosphate balance (A-B) was closed to 0 in all treatments except cow manure treatment (CMC), in which it was $+30kg\;ha^{-1}$ and show the possibility of over accumulation of phosphate by continuously replicated application of cow manure compost. Potassium balance was negative value in all but straw recycling treatment (FSC). It means that potassium was continuously supplied from soil minerals, uptaken by plants or eluted out of soil. In conclusion, by substituting inorganic fertilizer for organic fertilizer or reducing application rate of chemical fertilizer through mixing organic fertilizer, it would be possible to achieve the same rice yield as in the recommendation treatment and to decrease nutrient leaching below half level in rice paddy soil.

• Journal of Chest Surgery
• /
• v.38 no.8 s.253
• /
• pp.523-528
• /
• 2005

Background: Investigation of the change of ventricular function and mitral regurgitation after surgical repair of patient with anomalous origin of left coronary artery from the pulmonary artery (ALCAPA) is key issue for the better surgical outcome. Material and Method: From April 1986 to July 2002, 12 patients presented with ALCAPA. The median age at repair was 4 months. Surgical methods included left coronary artery transfer to the aorta (10), Takeuchi procedure (1), saphenous vein free graft bypass (1). Mitral valve was repaired in 1. Result: There were 2 hospital death $(16.7\%)$. The mean follow-up period was $7.1\pm4.1$ years (range, 7 months to 13 years). Four patients required postoperative circulatory assist for $2.2\pm1.1$ days and one needed left ventricular assist device (LVAD) for 1day. Postoperative echocardiography demonstrated significant improvements in mean fractional shortening $(33.4\pm9.1\%\;vs\;17.7\pm9.6\%,\;n=10,\;p<0.05);$ left ventricular end diastolic dimension $(33.4\pm7.3\;mm\;vs\;44.8\pm7.0\;mm,\;n=10,\;p<0.05)$ and systolic dimension $(22.2\pm7.5\;mm\;vs\;33.4\pm7.9\;mm,\;n=10,\;p<0.05)$. Severities of mitral regurgitation decreased in all survivors at 1st and 4th year follow-up echocardiography. There were 2 reoperation due to residual MR and right ventricular outflow obstruction (Takeuchi case). Conclusion: Anatomic repair of anomalous left coronary artery from the pulmonary artery offered an excellent surgical results, especially in terms of the recovery of left ventricle function and mitral regurgitation. However, preoperative indications for mitral procedure is to be evaluated.