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Antioxidant Activity of Lactic Acid Bacteria Isolated from Korean Traditional Food Kimchi (한국전통식품 김치로부터 분리한 유산균주의 항산화 활성)

  • Kim, Da-Young;Kim, Hong Seok;Yoo, Jung Sik;Cho, Yoon Ah;Kim, Cheol-Hyun
    • Journal of Dairy Science and Biotechnology
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    • v.38 no.2
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    • pp.89-98
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    • 2020
  • The purpose of this study was to investigate the probiotic properties of lactic acid bacteria (LAB) isolated from a Korean traditional food kimchi. Gram staining was performed by Macrogen (Macrogen, Inc.) for identification of the LAB. Five strains of LAB were identified, including DKGF9 (Lactobacillus plantarum), DKGF1 (L. paracasei ), DKGF8 (L. casei ), DK207 (L. casei ), and DK211 (L. casei ). The biological activities of the isolated strains were assessed. The results showed that heat resistance of the strains was similar to or higher than the commercial strain L. acidophilus LA-5. Indirect testing of the ability of the strains to attach to the mucin layer revealed that DKGF9, DKGF1, and DKGF8 have high binding affinities for the mucous layer. All strains showed antimicrobial activity similar to or higher than the commercial strain LA-5. In proteolysis experiments, the diameters of proteolysis zones of the five strains increased in the period of 24-72 h, with DKGF1 exhibiting the largest zone diameter. Three strains were selected based on their antioxidant activities. Among the five isolated strains, L. paracasei DKGF1 showed potential probiotic activity, and thus, it may be useful for the development of health-promoting products.

Development of Audio-visual Aids of Death Education for Hospice Patients and Their Families (호스피스 환자와 가족을 위한 임종교육 시청각 자료 개발)

  • Seo, Mi-Suk;Kang, Yu Jung;Yoon, Ji Yoon;Kim, Tae Yeon;Cho, Hye Jun;Park, So Yeon;Lee, Si Yeon;Jang, Ji Hye;Kim, Yu Jin;Kang, Mi Teum
    • Journal of Hospice and Palliative Care
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    • v.19 no.3
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    • pp.240-248
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    • 2016
  • Purpose: Patients and their caretakers need to understand various problems and requirements in the dying process so that they may prepare for death for the rest of their remaining life. Accordingly, a systematic audio-visual resource was developed to educate hospice patients and their families at the palliative care ward about the process of dying. Methods: For the development of an audio-visual resource, a initial education material was produced in the form of simple and accessible Power Point handouts based on literature study. Then, the program was completed through five rounds of a process, including expert advice, revision, update and evaluation. Results: The final version of the program was filmed with cooperation of the medical literature information division. Using the program, patients and families were educated through five phases over three sessions for a total 26 minutes and 34 seconds. Conclusion: The significance of this study lies in the fact that it was conducted after the establishment of the palliative care ward, which made it easier for nurses provide the education. It is expected that the program may be used by hospice specialists as well as nurses as an education resource for hospice patients and their families.

Polymorphism of the Helicobacter pylori feoB Gene and Clinical Correlation with Iron-deficiency Anemia in Korea (Helicobacter pylori feoB 유전자의 다형성과 철 결핍성 빈혈과의 관계)

  • Min, Kee-Woon;Jeon, Byung-Ha;Oh, Yoo-Joung;Choe, Yon-Ho
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.6 no.2
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    • pp.112-119
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    • 2003
  • Purpose: Helicobacter pylori infection is thought to be correlated with iron-deficiency anemia (IDA) at puberty. The H. pylori feoB gene, a high-affinity ferrous iron transporter, plays a central role in iron acquisition. This study aims to analyze the H. pylori feoB status according to the presence of antral gastritis with or without IDA. Methods: Fourteen H. pylori-positive patients aged from 10~18 years were categorized into subgroups based on the presence or absence of IDA. Eight patients had IDA, and the other six showed normal hematological findings. Genomic DNA was isolated from cultured H. pylori. Five sets of primers were used for PCR amplification of the feoB gene. The feoB region, 1.93 kb, was generated by linking of the PCR products and sequenced. The feoB gene sequences of H. pylori J99 and 26695 were used to compare with the clinical strains. Sequence comparisons of the feoB regions between the IDA (+) and (-) groups were performed. Results: Sequence analysis of the complete coding region of the feoB revealed 16 sites of polymorphism. Among these, 3 polymorphisms-Glu/Thr254Ala, Ile263Val, and Lys511Gln - were indigenous to Korean strains. Although statistically significant differences appear in 4 sites between IDA (+) and (-), the number of specimens are too low to assess the real differences. Conclusion: The 4 polymorphisms in the feoB gene seem to be related with IDA, but it is unclear yet because of small number of study strains. Further studies are required to prove the correlation of IDA and H. pylori infection.

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Seismic Behavior of Non-Seismic Concentrically Braced Frames with Shared Shear tab (쉬어탭 공유 접합부를 갖는 비내진중심가새골조의 내진거동)

  • Yeom, Hee Jin;Jung, Eun Bi;Yoo, Jung Han
    • Journal of Korean Society of Steel Construction
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    • v.27 no.3
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    • pp.323-332
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    • 2015
  • Special concentrically braced frames(SCBFs) have distinctive advantages in considerable seismic performance, which make engineers widely use SCBFs as lateral-load resisting systems in buildings and have researchers to develop SCBFs design methods. Compared to the extensive research of SCBF, comparatively little information is currently available on the performance of SCBFs designed and constructed before the early 1990's. Prior to 1988, concentrically braced frames(CBFs) design requirements were substantially less restrictive. As a result, many existing structures designed to these requirements may not ensure ductility and pose a significant concern in current buildings. In this study, these older frames are referred as non-seismic braced frames(NCBFs). In order to investigate the seismic behavior of NCBFs, finite-element(FE) models of SCBF and NCBF were suggested and verified using case investigation of NCBF conducted on the University of Washington. Using these models, the seismic behavior of NCBF with shared welding shear tab, which is the representative of the types of connections, was established and compared with the seismic performance of SCBF.

Studies on the Changes of Soil Salinity in the Kyehwa Saline Paddy Soil (계화도(界火島) 간척지(干拓地) 토양(土壤)의 연도별(年度別) 염분함량(鹽分含量) 변화(變化))

  • Hwang, Nam-Yul;Ryu, Jeong;Na, Jong-Seong;Oh, Dong-Hoon;Park, Keon-Ho;Choi, Bong-Ju
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.4
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    • pp.265-271
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    • 1991
  • This study was crried out in order to changes of soil salinity in the Kyehwa saline paddy soil from 1978 find out the to 1988. Surveyed soil was Munpo, Gwanghwal, Yeompo, Hasa series and distribution ratio of those area was 51.2%, 16.6 %, 30.2 %, 1.0 % respectively on the 2,500ha. In the cultivated field, the ratio of desalinization was increased in accordance with rice cultivating years but desalization was not conduct after six years in the uncultivated field. Soil salinity of Summer(during cultivation) and Fall(after cultivation) were 52.3 %, 62.5 % respectively as compare with Spring(before cultivating) and about changes of soil salinity according to different soil depth, underground layer 20-40cm and 40-60cm were raised the rate 28.4 % and 66.2 % in accordance with top soil.

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Clinial Analysis of Surgical Management for Descending Necrotizing Mediastinitis (하행 괴사성 종격동염의 수술적 치료에 대한 임상적 고찰)

  • Yu, Jeong-Hwan;Lim, Seung-Pyung;Lee, Seok-Ki;Kim, Yong-Ho;Kim, Si-Wook;Kang, Shin-Kwang;Yu, Jae-Hyeon;Lee, Young
    • Journal of Chest Surgery
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    • v.41 no.4
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    • pp.463-468
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    • 2008
  • Background: Descending necrotizing mediastinitis (DNM) is a life-threatening cervico-mediastinal infection extending from the oropharynx or periodontal space. We reviewed clinical outcomes of DNM patients that underwent surgical management. Material and Method: We analyzed the demographic and surgical data from 8 patients (6 males and 2 females) that underwent surgical management for DNM between August 2003 and August 2007. Result: The mean age was $56.6{\pm}12.3$ ($34{\sim}72$) years, Types of DNM were I (n=2), IIA (n=1), and IIB (n=5), based on the classification system of Endo et al. Four patients were septic at the time of operation. The infectious organism was identified in three cases and turned out to be Streptococcus. ICU stay was $24.3{\pm}17.9$ ($3{\sim}58$) days, and hospital stay was $49.1{\pm}33.8$ ($20{\sim}125$) days, There were two deaths (25%), both of which were due to multi-organ failure. Conclusion: Despite aggressive surgical drainage and appropriate medical management, DNM still had a high mortality rate, Early diagnosis and prompt surgical intervention are key to DNM management. In addition, transcervical drainage should be used in limited disease only.

Passive Alignment of Photodiode by using Visible Laser and Flip Chip Bonding (가시광 레이저를 이용한 수광소자의 수동정렬 및 플립칩본딩)

  • Yu, Chong-Hee;Lee, Sei-Hyoung;Lee, Jong-Jin;Lim, Kwon-Seob;Kang, Hyun-Seo
    • Journal of the Microelectronics and Packaging Society
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    • v.14 no.3
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    • pp.7-13
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    • 2007
  • In the optical module for optical communications, the flip chip bonding is used fer the precise alignment of the optical fiber and optical device. In flip chip bonding, the optical device is aligned and welded while observing the alignment mark of substrate and chip by using flip chip bonder in order to bond the optical device at the exact position. In this research, optical passive alignment method of photodiode(PD) flip chip bonding is suggested for low cost optical subassembly. By using the visible He-Ne laser (633nm wavelength), photodiode is easily aligned with emitting spot on the optical fiber with the help of stereoscopic alignment system. We compensated wavelength dependent deviation about 4m to find out real alignment position of 1550nm input laser by ray tracing. The maximum optical coupling efficiency between the optical fiber and photodiode was about 23.3%.

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The effects of music therapy on vital signs and pulsatile oxygen saturation of pediatric intensive care unit children (음악을 이용한 간호중재가 중환자실 환아의 활력징후와 산소포화도에 미치는 영향)

  • Yoo Cheong-Suk;Song Kei-Hee
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.6 no.3
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    • pp.382-396
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    • 1999
  • This study was attempted to prove the effect of emotional stability and vital signs applying music therapy program to the children admitted in the PICU. Data were collected from July to September, 1997. The subjects were 30 patients admitted in the PICU of 'S' University Hospital which were divided into two groups of experimental and control. Each group had 15 subjects. Method was nonequivalent control group pretest-postteset repeated design, observing vital signs and activity of subjects prior, during, and after the music intervention. The study tools were cassette tapes of 'Mother's music whose babies want to listen' and Space-lab patient monitor. Data were analyzed using the $SPSS/PC+;x^2$ test and t-test to analyze of the general characteristics ; paired t-test to prove hypotheses. Result were as follows; 1. Infants lower than seven months showed changing into stable vital signs from applying the music therapy, however infants from eight months to three-year old showed no change in vital signs. 2. Vital signs changed to stabilized condition in infants lower than seven months were heart rate and respiration rate. 3. The stability of vital signs during music therapy turned back to the previous state while terminating music therapy. 4. The effect of music therapy in the state of activity had on both infants group of lower than seven months and from eight months to three-year old, particularly more effective in the later group. I recommend follows on the base of above results ; 1. As above results shows, listening to music is effective on infants and toddler, intervention with music therapy appropriate to chidlren's age is hot recommended. 2. Comparative study with noise blocking effect and music therapy effect within the ICU environment be recommended. 3. The repeated study on when the exact time is and how many repeat the music therapy to show the above mentioned effect be recommended. 4. We recommend this music therapy to be done in the recovery room, isolating room, operating room as well as ICU.

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Influence of the Sonic Power Toothbrush on Reduction of Gingival inflammation and on the Amount of interleukin-6, Prevotella intermedia and Actinobacillus actinomycetemcomitans in Periodontal Pocket (치주낭 내의 치은 염증의 감소와 Interleukin-6, Prevotella intermedia 및 Actinobacillus actinomycetemcomitans에 대한 전동 칫솔의 효과)

  • Hong, Ji-Youn;Chae, Gyung-Joon;Jung, Sung-Won;Um, Yoo-Jung;Choi, Seong-Ho;Kim, Chong-Kwan
    • Journal of Periodontal and Implant Science
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    • v.37 no.sup2
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    • pp.409-426
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    • 2007
  • 세균성 치태는 치은의 염증과 치주 조직 파괴를 동반하는 치주염의 주요한 인자로서 치주 조직 건강을 유지하기 위하여 적절한 치태 조절이 필요하다. 본 논문의 목적은 12주 동안 만성 초기 및 중등도 치주염 환자에서 치은염에 대한 임상 지수의 감소, interleukin-6 (IL-6) 농도와 치주질환 원인균인 Prevotella intermedia (P. intermedia), Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans)에 대한 소니케어 전동 칫솔의 효과를 일반 칫솔과 비교해 보고자 하는 데 있다. 총 82명의 환자를 선택하였으며, 30명은 일반 칫솔, 52명은 소니케어 전동 칫솔 군으로 분류하여 칫솔질 교육을 실시하였다. 전악을 전치부와 구치부로 나누어 초진, 1, 4, 12주에서의 치태, 치은 지수 및 탐침 시 출혈 여부를 조사하였으며, 가장 깊은 치주낭 탐침을 보이는 치아 3개를 선택하여 탐침 깊이와 부착 정도를 측정하였고, 선택된 치아에서 초진, 1, 12주에 채취된 샘플을 통해 치은열구액 내의 IL-6 농도와 P. intermedia, A. actinomycetemcomitans의 CT값을 추가적으로 조사하여 다음과 같은 결과를 얻었다. 1 소니케어 전동 칫솔과 일반 칫솔군 모두 치은 염증을 나타내는 임상 지수 (치태지수, 치은지수, 탐침 시 출혈)는 12주 기간 동안 유의한 감소 (p<0.05)를 보였으나, 전동 칫솔 군에서 통계학적으로 더욱 유의하게 (p<0.05) 나타났다. 2. 전치부를 제외한 구치부 치아에서 소니케어 전동 칫솔군은 12주 기간 동안 탐침 시 출혈의 감소가 통계학적으로 유의하게 (p<0.05) 나타났다. 3. 가장 깊은 치주낭 탐침 깊이를 보이는 3개의 선택된 치아에서 치주낭 탐침 깊이와 부착 정도는 두 군 모두 초진에 비해 유의한 감소 (p<0.05)를 보였다. 퍼센트 변화 비교에서 치주낭 탐침 깊이는 소니케어 전동 칫솔군이 $18.47{\pm}10.05%$, 일반 칫솔군이 $14.19{\pm}8.16%$로, 부착 정도는 소니케어 전동 칫솔군이 $24.26{\pm}12.51%$, 일반 칫솔군이 $15.65{\pm}9.92%$로 각각 나타났으나, 군 간 통계적 유의차는 보이지 않았다. 4. 치은열구액의 IL-6 농도는 두 군 모두 12주 기간 동안 통계적으로 유의한 감소 (p<0.05)를 나타내었다. 퍼센트 변화 비교에서 전동 칫솔군은 51%, 일반 칫솔군은 37%로 각각 나타났으나, 군 간 통계적 유의차는 보이지 않았다. 5. Prevotella intermedia, Actinobacillus actinomycetemcomitans의 관찰에서 두 군 모두 유의한 차이는 없었다. 위 결과를 통해 본 연구에서는 소니케어 전동 칫솔의 사용이 일반 칫솔에 비하여 만성 초기 및 중등도 치주염 환자에서 치태의 제거, 치은 염증 및 임상 지수의 감소에 유의한 효과가 있으며 IL-6의 감소 경향에도 효과가 있음을 관찰하였다.

Trans-Korea Rail Logistics Strategy for the Normalization of South and North Korea Economic Cooperation (남북경제협력 정상화를 대비한 철도 물류망 구축 방안)

  • Kim, Jeong Hyun;Kim, Gang Seog;Kim, Yong Jin;Yu, Jeong Whon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.36 no.3
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    • pp.511-520
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    • 2016
  • It is essential to connect the railroads between South and North Korea in order to the implementation of the Eurasia initiatives and prepare for the normalization of the economic cooperation between south and north Korea as well as the unification. This study provides the strategy to establish the rail logistics network for the normalized trades between south and north Korea, and the accommodation of the logistics demands to China and Russia. The alternative routes were designated and the costs for the rehabilitation were estimated, and suggested the priority for the investment. The Trans-China Rail is prior to the Trans-Siberia Rail in terms of the utility for the logistics and the political value. In connection to the TSR, it is desirable to pass the Seoul-Gyeonggi area where the demand is greater than the other area. This route is limited by the restriction in the capacity, then the Gyeonggi Ring Rail route may be necessary.