• Title/Summary/Keyword: 유전특성

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Development of a New Synthetic Korean Native Chicken Breed using the Diallel Cross-Mating Test (토종닭의 이면교배조합 시험을 이용한 신품종 종계 개발)

  • Sohn, Sea Hwan;Choi, Eun Sik;Kim, Ki Gon;Park, Byeongho;Choo, Hyo Jun;Heo, Jung Min;Oh, Ki Suk
    • Korean Journal of Poultry Science
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    • v.48 no.2
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    • pp.69-80
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    • 2021
  • We conducted a 4 × 4 diallel cross-mating test using 934 chickens from four grandparent stock lines to develop a new synthetic breed of Korean native chicken. The mean values, combining ability, and reciprocal effects on survival rate, body weight, and hen-day egg production were analyzed. In phenotypes, most chickens have yellowish-brown, reddish-brown and mixed color feathers. The average survival rate was 86.8±12.3%, with the highest in YH combination. Specific combining ability (SCA) had a greater effect on survival rate than general combining ability (GCA), and the SCA of HY combination was the highest. The 16 cross-combinations were distinctly divided into three weight groups according to their genetic characteristics. At 12 weeks of age, GCA showed a greater effect on weight than SCA, and the SCA of FH combination was the highest. The age at first egg laying was 157 days, and the crosses reached sexual maturity faster than the pure lines. The egg production rate was highest in SY at 79.5±2.1%. The GCA and SCA for hen-day egg production were similar, and the SCA was highest in the HS and FY combinations. The reciprocal effect showed that the offspring's egg production rate was high when S and Y were maternal parents in almost all combinations. In conclusion, FH and HF combinations, which have excellent growth performance, are the most desirable paternal parent stock strains, and FY, FS, HY, and SY combinations, which have excellent laying performance with moderate weight, are the preferred maternal strains.

Mass Proliferation of Hibiscus hamabo Adventitious Root in an Air-lift Bioreactor, and the Antioxidant and Whitening Activity of the Extract (생물반응기를 이용한 황근 부정근의 대량증식과 추출물의 항산화 및 미백 활성 평가)

  • Lee, Jong-Du;Hyun, Ho Bong;Hyeon, Hyejin;Jang, Eunbi;Ko, Min-Hee;Yoon, Weon-Jong;Ham, Young Min;Jung, Yong-Hwan;Choi, Hwon;O, Eu Gene;Oh, Daeju
    • Korean Journal of Plant Resources
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    • v.35 no.4
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    • pp.435-444
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    • 2022
  • Hibiscus hamabo Sieb. et Zucc. (yellow hibiscus) is a deciduous semi-shrub plant and mainly growing in Jeju Island. This is known the unique wild hibiscus genus and classified as an 2nd grade of endangered plant for Korean Red List. In previous studies, properties of germination, ecological, genetical and salt resistance have been reported. In this study, we investigated mass-proliferated adventitious root using bioreactor, antioxidant and whitening effects to conduct functional ingredients. Yellow hibiscus were collected from Gujwa, Jeju by prior permission and they were introduced by explant type and various medium composition after surface sterilization. As a result, seed response rates were evaluated at range of 51.17~51.83%, in terms of comprehensive efficiency of shoot and root formation. In the case of adventitious root propagation condition was confirmed in half strength Murashige and Skoog medium salts, 30 mg/L sucrose, and 2 mg/L indole-3-butyric acid for 8 weeks in 5,000 mL bioreactor. We also compared between relationship with biomass and secondary metabolites accumulation by total phenolics content, the flavonoid content, DPPH free radical scavenging activity and melanin content. The results indicated that adventitious root mass proliferation, antioxidant and whitening effect could develop value of the high-quality cosmeceutical ingredient and further metabolite studies.

Response of Yield and Quality in Major Domestic Rice (Oryza sativa L.) Varieties according to the Nitrogen Application Levels (질소시비수준에 따른 국내 주요 벼 품종의 수량 및 품질 반응)

  • Jong-Seo Choi;Jinseok Lee;Shingu Kang;Dae-Woo Lee;Woonho Yang;Seuk-Ki Lee;Su-Hyeon Sin;Min-Tae Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.67 no.4
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    • pp.342-361
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    • 2022
  • In order to evaluate the effect of nitrogen application levels on yield and quality of rice varieties, a field experiment was conducted at National Institute of Crop Science of Korea from 2018 to 2020. Five levels (0, 3, 5, 7, and 9 kg/10a) of nitrogen fertilizer were treated to 21 Korean rice varieties. Yield, yield component, appearance quality, and protein content in rice were analyzed. The average head rice yield for 3 years decreased by 28%, 22%, 11%, and 8%, respectively, when cultivated with 0, 3, 5, and 7 kg/10a nitrogen application compared to cultivation with a standard nitrogen application amount, 9 kg/10a. The number of panicles per hill increased as the amount of nitrogen application increased, but there was no significant change in the number of grains per panicle and 1000-grains weight, and the number of panicles per hill showed relatively small annual variation compared to other yield components. There was no significant difference in the head rice ratio according to the nitrogen application amount, the broken rice ratio slightly decreased, and the floury rice ratio increased. The protein content of rice decreased with increasing nitrogen application in 2018 and 2019, and was the lowest at 7 kg/10a of nitrogen application, and showed a tendency to increase again at 9 kg/10a. In the case of 2020, as the amount of nitrogen application increased, the protein content showed a tendency to continuously increase. In terms of varieties, 13 varieties, including Chilbo, seemed to be capable of low-nitrogen cultivation because loss of the head rice yield was less and the protein content could be lowered to 6% or less according to 7 kg/10a nitrogen application.

Effects of Different Music Genres on the Stress Levels of Jeju Crossbred Horses (마방에서 음악장르에 따른 Jeju crossbred의 스트레스 변화 연구)

  • Yoonjeong Jang;Jae-Young Choi;Jongan Lee;Yongjun Kang;Nayoung Kim;Minjung Yoon;Moon-Cheol Shin;Sang-Min Shin;Sangsoo Sun;Jiwoong Lee
    • Journal of Life Science
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    • v.33 no.12
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    • pp.995-1001
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    • 2023
  • This study investigated the effects of three music genres (classical, new age, and rock) on the stress levels of six Jeju crossbred horses (Jeju horse × Thoroughbred) in a horse stable. The horses were exposed to the three genres for seven days, and their stress levels were measured by analyzing physiological markers, including neurotransmitter (cortisol, β-endorphin, dopamine, serotonin, and oxytocin) plasma levels and creatine phosphokinase (CPK) and aldolase serum levels. The neurotransmitter analysis showed significant differences in cortisol levels between classical and new age music exposure. Dopamine levels decreased significantly only with new age exposure. Although there were no significant differences in β-endorphin levels between the three genres, β-endorphin levels decreased with increasing classical and new age music playback times and increased with increasing rock music playback times. There were no significant differences in serotonin levels between the three genres. Oxytocin levels decreased significantly with exposure to classical and rock music. The CPK and aldolase analyses showed that CPK levels decreased significantly only with exposure to new age music and increased after playback ended, while aldolase levels decreased significantly with classical and new age music exposure and increased after playback ended. These findings suggest that classical music and new age are the optimal music genres for the psychological stability of Jeju crossbred horses. Playing back an appropriate music genre could be used to improve breeding and promote the welfare of Jeju crossbred horses.

Comparison between Uncertainties of Cultivar Parameter Estimates Obtained Using Error Calculation Methods for Forage Rice Cultivars (오차 계산 방식에 따른 사료용 벼 품종의 품종모수 추정치 불확도 비교)

  • Young Sang Joh;Shinwoo Hyun;Kwang Soo Kim
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.3
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    • pp.129-141
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    • 2023
  • Crop models have been used to predict yield under diverse environmental and cultivation conditions, which can be used to support decisions on the management of forage crop. Cultivar parameters are one of required inputs to crop models in order to represent genetic properties for a given forage cultivar. The objectives of this study were to compare calibration and ensemble approaches in order to minimize the uncertainty of crop yield estimates using the SIMPLE crop model. Cultivar parameters were calibrated using Log-likelihood (LL) and Generic Composite Similarity Measure (GCSM) as an objective function for Metropolis-Hastings (MH) algorithm. In total, 20 sets of cultivar parameters were generated for each method. Two types of ensemble approach. First type of ensemble approach was the average of model outputs (Eem), using individual parameters. The second ensemble approach was model output (Epm) of cultivar parameter obtained by averaging given 20 sets of parameters. Comparison was done for each cultivar and for each error calculation methods. 'Jowoo' and 'Yeongwoo', which are forage rice cultivars used in Korea, were subject to the parameter calibration. Yield data were obtained from experiment fields at Suwon, Jeonju, Naju and I ksan. Data for 2013, 2014 and 2016 were used for parameter calibration. For validation, yield data reported from 2016 to 2018 at Suwon was used. Initial calibration indicated that genetic coefficients obtained by LL were distributed in a narrower range than coefficients obtained by GCSM. A two-sample t-test was performed to compare between different methods of ensemble approaches and no significant difference was found between them. Uncertainty of GCSM can be neutralized by adjusting the acceptance probability. The other ensemble method (Epm) indicates that the uncertainty can be reduced with less computation using ensemble approach.

Phenotypic Variation in the Breast of Live Broiler Chickens Over Time (시간에 따른 생축 육계 가슴살의 표현형 변이)

  • Ji-Won Kim;Chang-Ho Han;Seul-Gy Lee;Jun-Ho Lee;Su-Yong Jang;Jeong-Uk Eom;Kang-Jin Jeong;Jae-Cheol Jang;Hyun-Wook Kim;Han-Sul Yang;Sea-Hwan Sohn;Sang-Hyon Oh
    • Korean Journal of Poultry Science
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    • v.51 no.2
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    • pp.97-106
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    • 2024
  • This study utilized the non-invasive MyotonPRO® device to analyze the stiffness in breast muscles of commercial broilers (Ross 308 and Arbor Acres) and compared these findings with data reported for Ross 708, where Woody Breast (WB) symptoms had been previously documented. The research revealed that Ross 308 and Arbor Acres displayed relatively lower stiffness values compared to Ross 708, suggesting a lack of WB expression. These results indicate differentiation in breast muscle traits across strains and underscore the necessity for further research into factors influencing WB manifestation. The study also measured additional muscle tone characteristics such as Frequency, Decrement, Relaxation, and Creep across various growth stages (2, 4, 6, and 8 weeks), finding significant variations with pronounced severity at weeks 2 and 8. An increase in stiffness was observed as the broilers aged, pointing to potential growth-related or stress-induced changes affecting WB severity. A strong positive correlation was established between increased breast meat weight and WB severity, highlighting that heavier breast meat could exacerbate the condition. This correlation is vital for the poultry industry, suggesting that weight management could help mitigate WB effects. Moreover, the potential for genetic selection and breeding strategies to reduce WB occurrence was emphasized, which could aid in enhancing management practices in commercial poultry production. Collectively, these insights contribute to a deeper understanding of WB in broilers and propose avenues for future research and practical strategies to minimize its impact.

Identifying Distribution Areas and Population Sizes for the Conservation of the Endangered Species Odontobutis obscura (멸종위기종 남방동사리의 보전을 위한 상세 분포 지역 및 개체군 크기 파악)

  • Jeong-Hui Kim;Sang-Hyeon Park;Seung-Ho Baek;Chung-Yeol Baek
    • Korean Journal of Ecology and Environment
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    • v.57 no.2
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    • pp.102-110
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    • 2024
  • This study presents a fine scale distribution of the endangered species, Odontobutis obscura, through field surveys and literature reviews. Using the mark-recapture method, the population size in major habitats was determined. Field surveys conducted on 18 streams in Geoje Island revealed that the O. obscura was only found in the main streams and tributaries of the Sanyang, Gucheon, and Buchun Streams, which are part of the Sanyang Stream watershed. The O. obscura exhibited relative abundances ranging from 0.5% to 35.3% at different locations, with certain spots showing higher relative abundances (18.8% to 35.3%), indicating major habitat areas. A review of six literature studies confirmed the presence of the O. obscura, although there were differences in occurrence status depending on the purpose, scope, and duration of the studies. Combining the results of field and literature surveys, it was found that the O. obscura inhabits the main and tributary streams of the Sanyang, Gucheon, and Buchun Streams, from the upper to lower reaches. Currently, the O. obscura population in the Sanyang Stream watershed maintains a stable habitat, but its limited distribution range suggests potential issues such as genetic diversity deficiency in the long term. The population size of the O. obscura was confirmed at two specific locations, with densities of 0.5 to 1.5 individuals per m2. The average movement distance from the release point was 13.1 m, indicating the limited mobility characteristic of ambush predators. Understanding the distribution and population size of endangered species is the first step towards their conservation and protection. Based on this information, further research could significantly contribute to the conservation of the O. obscura.

Investigation of fish community structure and species diversity in two river estuary ecosystems, the Taehwa River and Changwon Stream, based on conventional survey and eDNA metabarcoding (어구조사 및 환경 DNA 메타바코딩을 이용한 태화강, 창원천 하구 생태계의 어류 군집 구조 및 종 다양성 평가)

  • Hee-kyu Choi;Yu Rim Kim;Soon Young Hwang;Yeounsu Chu;Pyoungbeom Kim;Hyuk Je Lee
    • Korean Journal of Environmental Biology
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    • v.41 no.4
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    • pp.637-656
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    • 2023
  • River estuaries are dynamic and productive ecosystems with high regional biodiversity. Environmental DNA (eDNA) has become a useful approach to assessing biodiversity in aquatic ecosystems. This study was conducted to investigate fish community characteristics and species diversity in two river estuary ecosystems, the Taehwa River and Changwon Stream. We further compared conventional and eDNA metabarcoding analyses of the fish communities. The conventional survey was performed in May, July, and October 2022, while the eDNA analysis was conducted only in May. We observed various fish species with different life histories, including carp, goby, and marine fish. We also found that migratory fish, such as dace Tribolodon hakonensis, sweetfish Plecoglossus altivelis, and eel Auguilla japonica, occurred in the Taehwa River, suggesting high river connectivity. Marine fish species were predominant in the Changwon Stream, as this river is located close to the sea. The diversity indices showed that the Taehwa River generally had higher species richness, evenness, and diversity values than the Changwon Stream. A total of 9-19 species were detected in the conventional survey for the three sites, whereas 11-18 species were found from eDNA analysis. The findings indicate that the sensitivity of eDNA was similar to or higher than that of the conventional method. Our study findings suggest the efficiency and efficacy of eDNA-based fish community monitoring, although with some shortcomings in applying the genetic marker to Korean fish, including no clear-cut distinction for Korean endemic species and/or genetically closely related species groups.

A Study for Kinetics and Oxidation Reaction and Alcohols by (C4H4N2H)2Cr3O10 ((C4H4N2H)2Cr3O10에 의한 알코올들의 산화반응과 반응속도 연구)

  • Young-Cho Park;Jae-Ho Sim
    • The Journal of the Convergence on Culture Technology
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    • v.10 no.6
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    • pp.781-788
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    • 2024
  • (C4H4N2H)2Cr3O10 has been prepared by the reaction of C4H4N2 with CrO3 in water. (C4H4N2H)2Cr3O10 was identified by elemental analysis, IR, UV-Vis and TG-DTA-TMA system. The oxidation of benzyl alcohol by (C4H4N2H)2Cr3O10 in organic solvents showed that the reactivity increased with the increase of the dielectric constant. The oxidation of alcohols was examined by (C4H4N2H)2Cr3O10 in CH2Cl2. As a resuit, (C4H4N2H)2Cr3O10 was found as efficient oxidizing agent that converted benzylic alcohol, allylic alcohol, saturated primary alcohol, secondary alcohols to the corresponding aldehydes or ketones(70%~96%). The selective oxidation of alcohols was also examined by (C4H4N2H)2Cr3O10 in CH2Cl2. (C4H4N2H)2Cr3O10 was selective oxidizing agent(10%~96%) of benzylic alcohol, allylic alcohol, saturated primary alcohols in the presence of secondary ones. In the presence of DMF. solvent with acidic catalyst such as HCl. (C4H4N2H)2Cr3O10 oxidized benzyl alcohol and its derivatives. The Hammett reaction constant was -0.70(303K). The observed experimental data were used to rationalize the hydride ion transfer in the rate determining step.

Breeding of New Ever-bearing Strawberry 'Jinha' with High Soluble Solid Content (당도가 높은 사계성 딸기 '진하' 육성)

  • Jong Nam Lee;Jong Taek Suh;Su Jeong Kim;Ki Deog Kim;Hye Jin Kim;Mi Za Choi;Bok Rye Yun;Hwang Bae Shon;Yul Ho Kim;Su Young Hong
    • Korean Journal of Plant Resources
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    • v.37 no.4
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    • pp.386-391
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    • 2024
  • 'Jinha' is a new strawberry (Fragaria × ananassa Duch.) cultivar, which was released by the Highland Agriculture Research Institute in 2019. The 'Jinha' cultivar originates from a 2011 cross between 'Albion' and 'Goha,' both of which exhibited excellent ever-bearing characteristics, including continuous flowering and large fruits under long-day and high temperature conditions. This new cultivar was initially named 'Saebong No. 11' after examining its characteristics and productivity during summer cultivation between 2012 and 2016. After regional adaptability tests, 'Jinha' was selected from 'Saebong No. 11' as an elite cultivar. The general characteristics of 'Jinha' include intermediate, elliptic leaves, and medium growth. The fruits are conical and of a red color. The plant height of 'Jinha' is simiar to that of the control variety, 'Flamenco', but it has a lot of number of leaves. The cluster length of 'Jinha' was 35.5 cm, 10.8 cm longer than 24.7 cm of the control variety. The number of flower clusters of 'Jinha' appeared 14.4, which was 4.1 more than that of 'Flamenco'. The average fruit weight of 'Jinha' was 10.1 g, which was 0.8 g heavier than that of 'Flamenco'. The soluble solid content of 'Jinha' was 10.2 °Brix, which was 2.0 °Brix higher than that of 'Flamenco'. The marketable yield of 'Jinha' was 25,931 kg·ha-1, 440% more than that of 'Flamenco' with 5,900 kg·ha-1. Therefore, the new cultivar of ever-bearing strawberry 'Jinha' is expected to be very popular in the export or bakery market because it is high soluble solid content and good shape.