• Title/Summary/Keyword: 유전적 계통

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Phylogenetic Analysis of Native Vigna sinensis in Korea Using DNA Sequence of Internal Transcribed spacer (ITS) Region (토종 갓끈동부의 ITS1, 5.8S 및 ITS2의 염기서열을 이용한 계통 분석)

  • Seo, Pil-Soo;Lee, Sook-Young;Shin, Yong Kook
    • Journal of Life Science
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    • v.27 no.3
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    • pp.351-354
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    • 2017
  • Cowpea (Vigna unguiculata (L.) Walp.) is recognized as a potential source of protein and other nutrients. The genus Vigna includes 100 wild species of plants. Especially, Vigna unguiculata includes annual cowpeas (ssp. unguiculata) and ten wild perennial subspecies. DNA sequence of internal transcribed spacer (ITS) region was determined for Vigna sinensis, one of native plant, which was found in recent but thought to have gone extinct in Korea. The seeds of Vigna sinensis used in this study were donated from Dong-Young Jo. The DNA sequence of ITS-5.8S-ITS2 for Vigna sinensis obtained from this study was deposited as Vigna sinensis AY195581 on GenBank of NCBI (National Center for Biotechnology Information). We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. DNA similarities among subspecies including Vigna unguiculata showed the range 98 to 100% in sequence-based phylogenetic analysis using total 507 base pairs of ITS1, 5.8S and ITS2. Vigna unguiculata and subspecies were grouped independently as one cluster from other Vigna species used in the phylogenetic analysis. In this study, based on the phylogenetic analysis using the ITS1-5.8S-ITS2 sequence of Vigna sinensis, it may be concluded to be classified to one of Vigna unguiculata substrains.

Genetical Studies on the Non-molting Allele Mutation in Bombyx mori (새로운 돌연변이 불면잠의 유전학적 연구)

  • 노시갑;토정랑굉
    • Journal of Sericultural and Entomological Science
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    • v.33 no.2
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    • pp.72-74
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    • 1991
  • The recessive lethal mutation 'non-molting of Nho' (symbol, ㎚n) was founded on one preservation stocks. All dwarf larvae continued to eat a few mulberry leaves and very slightly increasing body-size over a 7 days, and then died without entering into molt. Linkage experiments showed that ㎚n was linked with Knob(K) on the 11th linkage group. Precise localization of the gene was performed by mating with ㎚ gene. This mutant was confirmed to be allelic with the ㎚ gene located on the same chromosome.

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PC Cluster based Parallel Evolutionary Algorithm for the Service Restoration of Distribution System (PC 클러스터 기반 병렬 적응진화 알고리즘을 이용한 배전계통 고장복구)

  • Mun, Kyeong-Jun;Lee, Hwa-Seok;Park, Jun-Ho
    • Proceedings of the KIEE Conference
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    • 2005.07a
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    • pp.158-161
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    • 2005
  • 본 논문에서는 해집단을 다음세대로 진화시킬 때, 유전알고리즘과 진화전략을 동시에 사용하고, 적합도에 따라 복제하는 과정에서 유전알고리즘과 진화전략이 적용될 해집단의 비율이 적응적으로 변경되는 적응진화 알고리즘을 제안하였다. 또한 제안한 알고리즘을 실시간 적용하기 위해 PC 클러스터 시스템으로 병렬처리하여 최적해 탐색 성능 및 탐색속도를 개선하였다. 제안한 알고리즘을 실 배전계통 고장복구 문제에 적용해 본 결과, 유전 알고리즘 또는 진화전략을 단독으로 사용한 경우보다 제안한 방법이 더 빠른 시간내에 우수한 최적해를 탐색하였고, 병렬 연산의 수행 노드수 증가에 따라 최적해 탐색성능은 유지하면서 최적해 탐색시간을 상당히 단축시킴을 확인하였다.

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PC Cluster based Parallel Evolutionary Algorithm for the Reconfiguration of Distribution System (PC 클러스터 기반 병렬 적응진화 알고리즘을 이용한 배전계통 최적 재구성)

  • Mun, Kyeong-Jun;Lee, Hwa-Seok;Park, June-Ho
    • Proceedings of the KIEE Conference
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    • 2005.07a
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    • pp.162-165
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    • 2005
  • 본 논문에서는 해집단을 다음세대로 진화시킬 때, 유전알고리즘과 진화전략을 동시에 사용하고, 적합도에 따라 복제하는 과정에서 유전알고리즘과 진화전략이 적용될 해집단의 비율이 적응적으로 변경되는 적응진화 알고리즘을 제안하였다. 또한 제안한 알고리즘을 실시간 적용하기 위해 PC 클러스터 시스템으로 병렬처리하여 최적해 탐색 성능 및 탐색속도를 개선하였다. 제안한 알고리즘을 참고문헌의 배전계통 재구성 문제에 적용해본 결과, 유전 알고리즘 또는 진화전략을 단독으로 사용한 경우보다 제안한 방법이 더 빠른 시간내에 우수한 최적해를 탐색하였고, 병렬 연산의 수행 노드수 증가에 따라 최적해 탐색성능은 유지하면서 최적해 탐색 시간을 상당히 단축시킴을 확인하였다.

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The Effects of gl, gh and wx Gene on the Grain Yield and Yield Components of Rice Plant (수도의 gl, gh 및 wx유전자가 수도수량 및 수량구성요소에 미치는 영향)

  • 김현구
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.22 no.1
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    • pp.83-109
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    • 1977
  • Isogenic lines for glabrous leaf (gl), hull color(gh) and glutinous endosperm (wx) were used to clarify the effects of the gl, gh and wx gene on the grain yield and yield components in rice plant. Eight genotypes: glgl ghgh wxwx, glgl ghgh WxWx, glgl GH-wxwx, glgl GH-WxWx, GL-ghgh wxwx, GL-ghgh WxWx, GL-GH-wxwx and GL-GH-WxWx were evalutated in the backcrossed $F_2$ generation. Significant effects were measured for gl genotypes on heading date, gh genotypes on grain fertility, and wxwx genotypes on number of panicles per hill and 1, 000 grain weight. The grain yield of straw hull and non glutinous lines were higher than gold hull and glutinous lines. The interaction of three genes, gl, gh and wx was significant for heading date, but not significant in other characters. The average value of isogenic lines which were combined with two recessive genes and one dominant gene were lower and the C.V. value was higher than the isogenic lines which were combined with two dominant genes and one recessive gene in all characters except plant height and number of grains per plant. The average value of isogenic line combined with three recessive genes was lower and the C.V. was higher than the isogenic line combined three dominant genes in all characters measured.

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Phylogenetic analysis of Locusta migratoria (Orthoptera: Acridae) in Haenam-gun, Jeollanam-do, Korea using Two Mitochondrial Genes (마이토콘드리아 유전자 2개를 이용한 대한민국 전라남도 해남군 발생 풀무치 Locusta migratoria (메뚜기목: 메뚜기과)의 계통분석)

  • Kim, Young-Ha;Jung, Jin-Kyo;Lee, Gwan-Seok;Koh, Young-Ho
    • Korean journal of applied entomology
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    • v.55 no.4
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    • pp.459-464
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    • 2016
  • An outbreak of the migratory locust, Locusta migratoria, in the environment-friendly reclaimed plantations of forage crops in Sanyimyeon, Haenam-gun, Jellanam-do, Korea in August 2014 caused severe damages to various crops. Owing to its first occurrence in the Korean history, the causes underlying the outbreak and phase-transition of the migratory locust were not known. It is critical to establish the genetic relationship of the migratory locust in Sanyimyeon, Haenam-gun with the other previously reported strains in the world in order to understand the mechanisms responsible for its outbreak. The gene sequences of the 16S ribosomal RNA (rRNA) and displacement-loop (D-loop) of the mitochondria of various regional species of the migratory locust were used to perform the phylogenetic analysis. Our results suggested that the migratory locusts in Sanyimyeon, Haenam-gun are closely related with the Eurasian strains of the northern lineage. In future, these two mitochondrial genes can be used for elucidating the genetic population structures in migratory locusts in various regions. In addition, the sequence information of these genes can be used to enhance our understanding of the genetic basis of the outbreak of migratory locusts.

Development of specific single nucleotide polymorphism molecular markers for Angelica gigas Nakai (ITS 영역의 HRM 분석을 통한 참당귀(Angelica gigas Nakai)의 특이적 SNP 분자표지 개발)

  • Lee, Shin-Woo;Lee, Soo Jin;Han, Eun-Hee;Shin, Yong-Wook;Kim, Yun-Hee
    • Journal of Plant Biotechnology
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    • v.48 no.2
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    • pp.71-76
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    • 2021
  • Angelica is a perennial plant used widely for medicinal purposes. Information on the genetic diversity of Angelica populations is important for their conservation and germplasm utilization. Although Angelica is an important medicinal plant genus registered in South Korea, no molecular markers are currently available to distinguish individual species from other similar species in different countries, in particular, China and Japan. In this study, we developed single nucleotide polymorphism (SNP) markers derived from internal transcribed spacer regions of the nuclear ribosomal DNA to identify a distinct domestic species, Angelica gigas Nakai, via a high-resolution melting (HRM) curve analyses. We also performed HRM curve analysis of intentionally mixed genomic DNA samples from five Angelica species. Finally, we investigated A. gigas Nakai and A. sinensis using varying ratios of mixed genomic DNA templates. The SNP markers developed in this study are useful for rapidly identifying A. gigas species from different countries.

The Gene Effect of Culm Length and Ear Length Using Isogenic Lines in Barley (동질유전자계통을 이용한 보리의 간장 및 수장유전자효과)

  • Cho, Chang-Hwan;Lee, Eun-Sup;Sung, Yeol-Kyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.3
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    • pp.270-280
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    • 1988
  • This experiment was conducted to evaluate the main effects and pleiotropic effects of two comparable isogenic lines for culm and spike length in hulled barley and observed various agronomic characteristics, yield and yield components under the conditions of heavy fertilizer application and high planting densities. Three isogenic lines, culm, spike and culm and spike, were planted and to obtain basic data for improving high-yielding and quality of hulled barley at Experiment Farm, Dankook Univ., Cheonan city in 1985. Differences of culm length between two comparable isogenic lines, short culmed line (SCL) and medium culmed line (MCL), in isogenic lines for culm length showed highly significant differences. And differences of spike length between two comparable isogenic lines, short spike line (SSL) and medium spike line (MSL), in isogenic lines for spike length showed highly significant differences. Differences of culm and spike length between two comparable isogenic lines, short culmed and spike line (SCSSL) and medium culmed and spike line (MCMSL), short culmed and spike line (SCSSL) and long culmed and spike line (LCLSL), in isogenic lines for culm and spike length showed highly significant differences. Characteristics affected highly by the genes of culm length in isogenic lines for culm length were heading days, the 1st and 2nd internode length, No. of grains per spike, No. of spikes/m$^2$, and one litre weight. Characteristics affected highly by the genes of spike length in isogenic lines for spike length were No. of spike nodes, heading days, maturity days, thousand grain weight and one litre weight. Characteristics affected highly by the genes of culm and spike length in isogenic lines for culm and spike length were No. of spike nodes, the 2nd, 3rd and 4th internode length, thousand grain weight and grain yield. Grain yield of SCL, MSL, MCMSL and SCSSL was more than grain yield of MCL, SSL, SCSSL and LCLSL, respectively. Maturity days of SCL, MSL, SCSSL and LSLSL were faster than maturity days of MCL, SSL, MCMSL and SCSSL, respectively.

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혈액형지배 유전자에 의한 칡소의 유전적 특성

  • 조창연;연성흠;손동수;이호준;윤종택
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.50-50
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    • 2001
  • 혈액형을 지배하는 유전자는 진화에 대하여 중립적인 작용을 하고 있어서 집단의 유전적 구조의 특성 파악, 계통분류학 등에 많이 응용되고 있다. 본 연구는 칡소에 대한 유전학적 특성을 구명하고자 혈액형 분석기술을 응용하여 실시하였다. 공시동물은 (주)한경게놈텍 목장에서 사육중인 외모적으로 칡소의 특징을 보이는 25두를 이용하였다. 혈액은 경정맥에서 헤파린 처리된 진공 채혈관에 무균적으로 채취하여 혈장, 백혈구 및 적혈구로 원심분리한 후 냉동 혹은 냉장 보관하여 각 실험에 이용하였다. 적혈구 항원형의 검출은 2% 적혈구 부유액과 축산기술연구소에서 생산된 항혈청 11종을 이용하여 용혈반응으로 실시하였고, 혈액단백·효소를 지배하고 있는 6개의 유전자 좌위에 대하여 전분 혹은 포리아크릴 아미드겔 전기영동으로 다형 검출을 실시하였다. 용혈반응으로 검출한 적혈구 항원형의 반응양상은 검사한 11종의 항체에 대하여 6종은 50%이상의 개체에서 양성반응을 보였다. 이와 같은 결과는 일반 한우에서 보이는 양성반응율보다는 높은 것으로 판단되어진다. 전기영동법으로 분석한 6개의 혈액단백·효소 지배 유전자 좌위 중 ALB좌위을 제외한 5개 유전자 좌위에서 다형이 관찰되었다. HB, AMY-1, GC 및 PTF-2 유전자 좌위는 2개의 대립유전자가 관찰되었고, TF 유전자 좌위는 4개의 대립유전자가 관찰되었다. 표 1에서 같이 칡소에서 관찰된 각 유전자 좌위의 대립유전자 빈도의 구성은 일반적인 한우와는 상이한 결과를 보였으나 평균 이형접합도는 칡소가 0.438, 일반한우가 0.442로 계산되어 유전적 변이성은 유사한 것으로 추정되었다. 이상의 결과로 본 연구에서 분석한 칡소는 다른 한우집단과는 상이한 유전적 구조를 가지고 있으나, 유전적 다형성은 비교적 높은 것으로 시사되었다. 보다 정확하고 많은 량의 유전정보 수집을 위하여 Microsatellite DNA 및 모색 관련 유전자를 분석할 필요성이 있을 것으로 사료된다.(Table Omitted)

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Genetic Variations of Chicken TYR Gene and Associations with Feather Color of Korean Native Chicken (KNC) (한국 토종닭 모색 변이와 TYR 유전자형 간의 상관관계 분석)

  • Choi, Jin Ae;Lee, Jun-Heon;Jang, Hyun-Jun;Lee, Kyung-Tai;Kim, Tae-Hun;Lee, Hyun-Jeong;Heo, Kang-Nyeong;Kim, Chong-Dae;Han, Jae-Yong;Park, Mi Na
    • Korean Journal of Poultry Science
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    • v.41 no.1
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    • pp.7-14
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    • 2014
  • Tyrosinase (TYR) gene is located on chromosome 1 in chicken and it is composed of five exons and four introns. TYR gene is described as a key enzyme in melanin biosynthesis. Most examples of complete albinism in chicken have been due to defects in the tyrosinase gene. The association of feather color and sequence polymorphism in the Tyrosinase (TYR) gene was investigated using Korean Native chicken H breed (H_PL), Korean Native chicken L/W breed(L/W_PL) and 'Woorimatdag' commercial chickens (Woorimatdag_CC). From L_PL and W_PL breed analyses, 4 synonymous SNPs (locus G33A, G116A, C217T and C247T) and 2 SNPs (G838A and G958A) were detected in 4th exon and 4th intron of TYR gene respectively. The genotype frequencies for 6 SNPs were compared between L_PL and W_PL and W_PL represented homozygous SNP types in all the analyzed SNP positions while L_PL displayed various SNP types.