Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Development of specific single nucleotide polymorphism molecular markers for Angelica gigas Nakai

ITS 영역의 HRM 분석을 통한 참당귀(Angelica gigas Nakai)의 특이적 SNP 분자표지 개발

  • Lee, Shin-Woo (Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University) ;
  • Lee, Soo Jin (Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University) ;
  • Han, Eun-Hee (Gyeongsangnam-do Agricultural Research and Extension Services) ;
  • Shin, Yong-Wook (Department of Plant & Biomaterials Science, Chilam Campus, Gyeongsang National University) ;
  • Kim, Yun-Hee (Department of Biology Education, College of Education, IALS, Gyeongsang National University)
  • 이신우 (경상국립대학교 생명과학대학 항노화신소재과학과) ;
  • 이수진 (경상국립대학교 생명과학대학 항노화신소재과학과) ;
  • 한은희 (경상남도 농업기술원 약용자원연구소) ;
  • 신용욱 (경상국립대학교 생명과학대학 항노화신소재과학과) ;
  • 김윤희 (경상국립대학교 사범대학 생물교육과 (농업생명과학연구원))
  • Received : 2021.04.16
  • Accepted : 2021.05.11
  • Published : 2021.06.30
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Abstract

Angelica is a perennial plant used widely for medicinal purposes. Information on the genetic diversity of Angelica populations is important for their conservation and germplasm utilization. Although Angelica is an important medicinal plant genus registered in South Korea, no molecular markers are currently available to distinguish individual species from other similar species in different countries, in particular, China and Japan. In this study, we developed single nucleotide polymorphism (SNP) markers derived from internal transcribed spacer regions of the nuclear ribosomal DNA to identify a distinct domestic species, Angelica gigas Nakai, via a high-resolution melting (HRM) curve analyses. We also performed HRM curve analysis of intentionally mixed genomic DNA samples from five Angelica species. Finally, we investigated A. gigas Nakai and A. sinensis using varying ratios of mixed genomic DNA templates. The SNP markers developed in this study are useful for rapidly identifying A. gigas species from different countries.

당귀는 약용으로 널리 사용되는 대표적인 다년생 식물이다. 다양한 당귀 계통의 유전적 다양성에 대한 정보는 당귀의 유전자원의 발굴 및 보존의 차원에서 매우 중요하다. 당귀는 현재 한국에 등록 된 중요한 약용 식물 종이지만, 다른 나라의 다른 유사한 종과 구별 할 수 있는 분자표지가 없는 실정이다. 그러므로, 본 연구에서는 HRM 분석을 통해 국내 토종 당귀계통인 참당귀를 식별하기 위해 유전체 염기서열의 핵리보솜 DNA 내부(ITS)영역에서 단일염기다형성(SNP) 분자 표지를 개발하였다. 본 연구에서는 또한 5가지 당귀계통들의 혼합된 염색체 DNA 시료를 이용하여 HRM 분석을 수행하였으며, 최종적으로는 혼합된 염색체 DNA의 다양한 비율을 사용하여 혼용되는 대표적인 당귀계통인 참당귀와 중국 당귀를 재료로 HRM 분석을 진행하였다. 그러므로, 본 연구에서 개발된 SNP 분자표지는 다양한 지역 또는 국가에서 서식하는 당귀 계통들의 신속한 확인을 위해 매우 유용하게 이용될 것으로 생각된다.

Keywords

Acknowledgement

본 연구는 농림축산식품부 농생명산업기술개발사업(과제번호:314021-03-1-SB050)에 의해 이루어진 결과입니다.

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