• Title/Summary/Keyword: 유전자 지문감식기술

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Effect of forensic short wavelength UV on DNA (법과학 단파자외선이 DNA에 미치는 영향)

  • Kim, A-Ram;Kim, Woo-Joong;Park, Hee-Yeon;Lim, Si-Keun
    • Analytical Science and Technology
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    • v.27 no.3
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    • pp.147-152
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    • 2014
  • RUVIS(Reflective Ultraviolet Imaging System) is an effective equipment that detects the location of latent fingerprint at crime scene using short wavelength ultraviolet of 254 nm. In this study, the degree of DNA damage in biological samples was compared depending on the distance and time of processing using four commonly used RUVIS. 50% of DNA was damaged by treating 10 seconds at 10 cm distance in 3 types of RUVIS such as Police RUVIS, SIRCHIE mini light and SIRCHIE RUVIS. In addition, the degree of DNA damage was increased as the distance was closer and the treatment time was longer. It showed that short wavelength UV could cause DNA damage when used close to the samples at crime scene. Therefore, it was suggested to use RUVIS at a distance of at least 1 m. The degree of DNA damage was not significant by Polilight which used long wavelength ultraviolet of 350 nm. As a result, the choice and usage of which UV light and RUVIS were critical for detection of fingerprint and successful DNA typing.

Analysisof CCTV Privacy Security Trend for Intelligent Video Surveillance (지능형 영상감시를 위한 CCTV 프라이버시 보안 동향 분석)

  • Lee, Jae-Chul;Park, Seok-Cheon;Yang, Byeong-Ho
    • Proceedings of the Korea Information Processing Society Conference
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    • 2013.05a
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    • pp.268-271
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    • 2013
  • CCTV는 사람들 생활에서 없어서는 안 되는 유용한 존재로 자리매김 했다. 가장 큰 장점은 범죄 수사과정에서 유전자나 지문감식에 견줄 만큼 기여도가 크다는 점이다. 폭력 및 범죄를 해결하는데 유용하게 쓰이고 있고 날로 발전하고 있는 상황이다. 본 연구에서는 최근 공간정보기술의 발달에 따라 여러 분야에 공간 정보 기반의 관제시스템이 운영 중이거나 추진 중에 있으며, 지능형 영상감시를 위한 동향과 CCTV 설치에 따른 합법성과 프라이버시 보호에 대해 연구를 한다.

Culture-Independent Methods of Microbial Community Structure Analysis and Microbial Diversity in Contaminated Groundwater with Major Pollutants (주요 오염물질로 오염된 지하수에서 미생물의 무배양식 군집분석방법과 미생물상에 대한 조사방법 연구)

  • Kim Jai-Soo
    • Journal of Soil and Groundwater Environment
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    • v.11 no.3
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    • pp.66-77
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    • 2006
  • This review inquired the recently applied molecular biological and biochemical methods analyzing the microbial community structure of groundwater and, as a result, summarized the functional or taxonomic groups of active microorganisms with major contaminants in groundwater. The development of gene amplification through PCR has been possible to figure out microbial population and identification. Active microbial community structures have been analyzed using a variety of fingerprinting techniques such as DGGE, SSCP, RISA, and microarray and fatty acid analyses such as PLFA and FAME, and the activity of a specific strain has been examined using FISH. Also, this review included the dominant microflora in groundwater contaminated with fuel components such as n-alkanes, BTEX, MTBE, and ethanol and chlorinated compounds such as TCE, PCE, PCB, CE, carbon tetrachloride, and chlorobenzene.