• Proceedings of the Korean Society of Life Science Conference
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• 2003.05a
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• pp.109-109
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• 2003

• Proceedings of the Korean Society of Crop Science Conference
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• 2004.04a
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• pp.84-85
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• 2004

• Journal of Scientific & Technological Knowledge Infrastructure
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• s.3
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• pp.26-33
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• 2000

어떤 초능력을 가진 사람이 인간의 유전자 서열을 눈으로 볼 수 있어, 이것을 컴퓨터에 바로 타이핑을 해서 입력한다고 해도 무려 100년이 넘게 걸린다. 따라서 이 엄청난 일차 정보만을 저장하고 처리한다고 해도 이것은 보통 일이 아닐 수 없으며 컴퓨터 도움 없이는 가공이 아니라 간단한 이동도 불가능할 정도로 엄청난 양의 자료다.

• Proceedings of the Korean Society of Life Science Conference
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• 2002.03a
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• pp.71-71
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• 2002

• Proceedings of the Korean Society of Life Science Conference
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• 2002.03a
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• pp.48-48
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• 2002

• Korean Journal of Optics and Photonics
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• v.34 no.2
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• pp.45-52
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• 2023

In this study, we have investigated the effect of photobiomodulation (PBM) on corneal wound healing, using a low-power light-emitting diode (LED) at different wavelengths. We found that LEDs with wavelengths ranging from 623 to 940 nm had no significant cytotoxic effects on corneal epithelial cells. The effect of PBM on promoting cell migration was analyzed by scratch assay, and it was found that PBM at 623 nm significantly increased cell migration and promoted wound healing. Furthermore, the expression of genes related to cell migration and wound healing was analyzed, and it was found that PBM at 623 nm upregulated the expression of the genes FGF-1 and MMP2, which are known to promote cell proliferation and extracellular matrix degradation. These findings suggest that PBM with low-powered light at specific wavelengths, particularly 623 nm, could be utilized to treat corneal injury.

• Journal of Life Science
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• v.13 no.5
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• pp.723-731
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• 2003

For the investigation of conserved regions in lipase genes, 132 and 24 sequences were obtained from LED (Lipase Engineering Database) and COG (Clusters of Orthologous Groups of proteins), respectively. There was high diversity in lipase genes and peculiar amino acid sequences were conserved for each homologous family of LED. Similar conserved amino acid sequences were detected from COG0657 and Moraxella lipase 1 homologous group of LED. Although many studies have attempted to detect new lipase genes in procaryotes, they have been limited culturable bacteria. The importance of metagenome, including DNA from non-culturable bacteria, is known. Due to the high diversity, we assumed it might be possible to detect new lipase gene from metagenome. Due to the high diversity of nucleotide sequences in lipase genes, 10 primer sets were designed. Designed primer sets were inspected in BLAST of NCBI and they could amplify a part of the lipase gene from 222 to 713 bp. They can amplify 16.7%∼60.0% of each lipase homologous group which was 3.6 fold higher than each sets. They might offer a high probability of detecting new lipase genes, owing to high efficiency and the diversity of lipase genes.

• Journal of Science Criminal Investigation
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• v.11 no.3
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• pp.210-217
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• 2017

In the forensic microbiology laboratories, microorganism analyses from food are requested. There have been several cases of Bacillus cereus isolated from the samples requested to the National Forensic Service. B. cereus is an important pathogenic bacterium which can cause food-borne outbreaks. Therefore, we isolated B. cereus from anchovy aekjeot recently requested for microbial examination and identified using MSId based on the 16S rDNA sequence and real-time PCR method. We also conducted PCR for detection of diarrheal toxin genes and an emetic toxin gene and found the presence of nheABC, bceT and entFM diarrheal toxin genes in the B. cereus isolate. There are several clinically important food-poisoning bacteria that should be noted during inspection. In particular, B. cereus can cause food poisoning even when cooked foods are ingested, because B. cereus forms endo-spore which confers strong environmental resistance and heat resistance to the bacteria, and the bacterial emetic toxin also has heat resistance. Here we highlight the importance to distinguish clinically important bacteria such as B. cereus from food specimens, and we expect this study will provide procedures for identification of B. cereus and detection of the bacterial toxin genes for future cases in the forensic microbiology laboratories.

• Tuberculosis and Respiratory Diseases
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• v.40 no.6
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• pp.653-658
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• 1993

Background: Recent advancement of molecular genetics has revealed that malignant transformation of a cell may be a complex multistep process and this process is grouped, in general, into two distinct categories, activation of protooncogenes and inactivation of tumor suppressor genes. This study was focused on the mutation of p53 tumor suppressor gene, because p53 gene mutation is now generally accepted to be one of the most frequent genetic changes in a variety of human cancers. Although lung cancer is one of the common cancers in Korea, the genetic change in the carcinogenesis process is not yet known clearly. To investigate the role of p53 gene mutation in lung cancer, we examined the mutations of exon 4-8 of the p53 gene in humna lung cancer cell lines, because most of the mutations of p53 gene have been reported to develop in exon 4-8. Method: Genomic DNA was obtained by the digestion of proteinase K and the extraction by phenol-chloroform-ethanol method from two human pulmonary adenocarcinoma cell lines, PC-9 and PC-14, and one human small cell lung cancer cell line, H69. To detect the mutations of exon 4-8 of the p53 gene, polymerase chain reaction single-strand conformation polymorphism(PCR-SSCP) analysis was performed with the DNA extracted from the cells. Results: The mutation of p53 gene was present in all three cell lines tested. In PC-9, PC-14 and H69, the altered mobility was detected in exon 7, 7 and 5, respectively. Conclusion: These results suggest that p53 gene mutation plays an important role in certain steps of the carcinogenesis of human non-small cell and small cell lung cancer.

• Proceedings of the Korea Information Processing Society Conference
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• 2010.04a
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• pp.627-630
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• 2010

무선 센서 & 액터 네트워크(WSAN)와 같이 다수의 베이스 노드가 존재하거나 베이스 노드의 이동성이 큰 센서 네트워크에서 최소 Wiener수 신장 트리(MWST)기반 라우팅 방법은 최소 신장 트리(MST)기반 라우팅 방법에 비해 패킷 전송 거리가 짧고 전력 소모가 적다. 하지만 주어진 그래프로부터 최소 Wiener 수 신장 트리를 찾는 문제는 NP-hard 문제이고 최소 신장 트리에 비해 네트워크 수명이 짧은 단점이 있다. 본 논문은 이러한 문제를 해결하고자 Wiener 수 적응도, 네트워크 수명 적응도, 차수 적응도 등을 동시에 고려한 다목적 유전자 알고리즘을 설계하고 네트워크 전체 전력 소모를 크게 증가시키지 않으면서도 네트워크의 수명을 Wiener 수 적응도만을 사용했을 때 보다 연장시킴을 실험을 통해 보인다.