• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2005.10a
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• pp.123-126
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• 2005

본 연구는 고등동물의 불포화지방산 생합성의 핵심 효소로 알려져 있는 Stearoyl-CoA desaturase(SCD) 유전자의 특정한 단일염기다형(single nucleotide polymorphism; SNP)이 한우의 도체 및 육질형질에 미치는 영향을 분석하기 위해 SCD 유전자의 Intron 7번 영역의 특정부위를 포함하는 primer를 제작하고, 염기서열 분석을 통하여 유전자 구조를 해석한 결과 총 211bp 크기를 갖는 염기서열의 122번째에서 아데닌(A)${\leftrightarrow}$구아닌(G) 염기치환으로 발생한 단일염기다형(SNP) 부위를 발견하였다. 이들 단일염기다형 염기서열 부위를 PCR-SSCP(single-strand conformation polymorphism) 기법을 이용하여 분석한 결과 3종류의 SNP 유전자형(A/A, A/G 및 G/G)을 검출하였다. 이 가운데 A/G 유전자형이 한우의 근내지방도와 등지방두께와 고도의 유의적 연관성이 있다는 새로운 사실을 발견하였다. 따라서, 본 연구를 통해 개발된 한우 SCD 유전자의 특정한 단일 염기다형 표지인자는 한우의 연령 및 성별에 관계없이 육질이 우수한 고급육을 생산하는 우량 한우의 조기식별에 매우 유용한 DNA 표지인자로 활용할 수 있을 것으로 기대된다.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2005.11a
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• pp.68-69
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• 2005

To identify germline chimeric chicken using germ cell transplantation method, the testcross, spends much time, labor and cost to perform, is the only way for distinguishing germline chimeric chicken from normal one And to enhance the method, development of breed-specific molecular markers have been needed. We have just identified breed-specific sequence polymorphisms among Barred Plymouth rock, Korean Ogol Chicken and White Leghorn in PMEL17 and MC1R gene the loci of which are identical to dominant white and extended black loci. These sequence polymorphism will be very useful for screening germline chimera.

• Proceedings of the Zoological Society Korea Conference
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• 1996.10a
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• pp.67.2-67
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• 1996

• Proceedings of the Plant Resources Society of Korea Conference
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• 1997.10b
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• pp.36-36
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• 1997

• Journal of Animal Science and Technology
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• v.49 no.6
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• pp.719-728
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• 2007

The cysteine and glycine rich protein 3 (CSRP3), apolipoprotein B mRNA editing enzyme catalytic polypeptide‐like 2(APOBEC2) and caveolin (CAV) gene family(CAV1, CAV2, CAV3) have been reported to play important roles for carcass and meat quality traits in pig, mouse, human and cattle. As an initial step, we investigated SNPs in these 5 genes among eight different cattle breeds. Eighteen primer pairs were designed from bovine sequence data of NCBI database to amplify the partial gene fragments. Sequencing results revealed 9 SNPs in the coding regions of three caveolin genes, 1 SNP in CSRP3 and 3 SNPs in APOBEC2 gene. All the identified SNPs were confirmed by PCR-RFLP. Also, 9 more intronic SNPs were detected in these genes. However, all identified mutations in the coding region do not change amino acid sequence. Allelic distributions were significantly different for 5 SNPs in CAV2, CAV3, CSRP3 and APOBEC2 genes among the eight different breeds. These results gave some clues about the polymorphisms of these genes among the cattle breeds and will be useful for further searches for identifying association between these SNPs and meat quality traits in cattle.

• Proceedings of the Korean Aquaculture Society Conference
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• 2005.05a
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• pp.84-84
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• 2005

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.50-50
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• 2001

혈액형을 지배하는 유전자는 진화에 대하여 중립적인 작용을 하고 있어서 집단의 유전적 구조의 특성 파악, 계통분류학 등에 많이 응용되고 있다. 본 연구는 칡소에 대한 유전학적 특성을 구명하고자 혈액형 분석기술을 응용하여 실시하였다. 공시동물은 (주)한경게놈텍 목장에서 사육중인 외모적으로 칡소의 특징을 보이는 25두를 이용하였다. 혈액은 경정맥에서 헤파린 처리된 진공 채혈관에 무균적으로 채취하여 혈장, 백혈구 및 적혈구로 원심분리한 후 냉동 혹은 냉장 보관하여 각 실험에 이용하였다. 적혈구 항원형의 검출은 2% 적혈구 부유액과 축산기술연구소에서 생산된 항혈청 11종을 이용하여 용혈반응으로 실시하였고, 혈액단백·효소를 지배하고 있는 6개의 유전자 좌위에 대하여 전분 혹은 포리아크릴 아미드겔 전기영동으로 다형 검출을 실시하였다. 용혈반응으로 검출한 적혈구 항원형의 반응양상은 검사한 11종의 항체에 대하여 6종은 50%이상의 개체에서 양성반응을 보였다. 이와 같은 결과는 일반 한우에서 보이는 양성반응율보다는 높은 것으로 판단되어진다. 전기영동법으로 분석한 6개의 혈액단백·효소 지배 유전자 좌위 중 ALB좌위을 제외한 5개 유전자 좌위에서 다형이 관찰되었다. HB, AMY-1, GC 및 PTF-2 유전자 좌위는 2개의 대립유전자가 관찰되었고, TF 유전자 좌위는 4개의 대립유전자가 관찰되었다. 표 1에서 같이 칡소에서 관찰된 각 유전자 좌위의 대립유전자 빈도의 구성은 일반적인 한우와는 상이한 결과를 보였으나 평균 이형접합도는 칡소가 0.438, 일반한우가 0.442로 계산되어 유전적 변이성은 유사한 것으로 추정되었다. 이상의 결과로 본 연구에서 분석한 칡소는 다른 한우집단과는 상이한 유전적 구조를 가지고 있으나, 유전적 다형성은 비교적 높은 것으로 시사되었다. 보다 정확하고 많은 량의 유전정보 수집을 위하여 Microsatellite DNA 및 모색 관련 유전자를 분석할 필요성이 있을 것으로 사료된다.(Table Omitted)

• The Korean Journal of Zoology
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• v.38 no.3
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• pp.324-329
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• 1995

The phylogenetic relationships between Cheju native horses and Tsushima native horses were studied by protein polymorphism analyses in 16 gene loci (Trypsin inhibitor: Ti, Chymotrypsin inhibitor: CTi, Albumin: Al, Esterase: Es, Transferrin: Tf, Hemoglobin: Hb, Catalase: Cat, Esterase D: EsD, Glutamate oxaloacetate transaminase: GOT, Glyoxalase I: GLO I, Acid phosphatase: AcP, Superoxide dismutase: SOD, Lactate dehydrogenase: LDH, Hexokinase: HK, Malate dehydrogenase: MDH, Malic enzyme: ME). All allelic patterns of the protein loci, except 5 loci (SOD, LDH, HK, MDH, ME), were polymorphic in both two populations. Gene frequencies of the polymorphic loci of the population of Cheju native horses were higher than those of Tsushima native horses. Average heterozygosity in Cheju native horses was 0.375, showing higher than that of Tsushima native horses (0.304). The Da distance and gene identity of two populations were 0.108 and 0.868, respectively. The phylogenetic tree constructed by these results and those previously reported in other horse populations, consisted of three clusters. From this phylogenetic tree, it could be suggested that Cheju native horses and Tsushima native horses had diverged from the Mongolian wild horse (Equus prsewolskii).

• Tuberculosis and Respiratory Diseases
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• v.45 no.3
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• pp.529-535
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• 1998

Background: Interleukin-4 plays an important role in pathogenesis of asthma, especially in developing atopy by means of switching B lymphocytes to produce IgE. It has been shown that there is polymorphism in the Interleukin-4 promoter region, transversion of cytosine to thymine at-598 from translation initiation site of IL-4 gene. There has also been quite a few works to reveal the role of the polymorphism of IL-4 gene in patients with asthma. We performed this investigation to determine the role of the polymorphism in the severity of symptoms of patients with asthma. We also examined the frequency and the type of the polymorphism in asthmatics compared with non-asthmatics as well. Method: The subjects enrolled in this study were 49 asthmatics and 33 non-asthmatics. All the asthmatics were classified as mild and moderate to severe by the NHLBI/WHO Workshop. DNA from both asthmatics and non-asthmatics was extracted, then performed ARMS(Amplification Refractory Mutation System) as well as RFLP using BsmFl restriction enzyme in order to confirm the polymorphism of Il-4 gene. Results: There was no significant difference in the occurrence of polymorphism of the IL-4 promoter sequence between asthm and non-asthma groups(P=0.7). Among those with polymorphisms, the number of C/C type was slightly more than C/T type in both asthmatics and non-asthmatics, 26 vs 21 in asthmatics and 18 vs 15 in non-asthmatics, which was, however, insignificant statistically. No significant relationship between the severity of asthma and the polymorphism was found(P=0.7). Conclusion: There was no significant difference between the severity of asthma and the IL-4 promoter polymorphism(P=0.709). Interestingly, the frequency of the polymorphism in both asthmatics as well as non-asthmatics was found to be even higher than that occurred in Caucasians. However, no significant difference in the frequency of the polymorphism was found in both groups.

• Korean Journal of Poultry Science
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• v.36 no.1
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• pp.85-88
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• 2009

One of the mitochondrial genes, called cytochrome c oxidase I (COI), has been widely used for the species identification (called bio-barcode) in birds. In this study, the bio-barcode has been applied to chicken breeds in Korea whether it also can be used as a molecular marker for breed identification. Data indicated that Korean native chicken has the mixed SNP (single nucleotide polymorphism) patterns between White Leghorn (Layer) and Cornish (Broiler) and ultimately, it can not be used as the marker for breed identification. However, this result indicates the mixed use of the Korean native chicken, since it has been used for dual purpose for producing meat and egg for a long time. In order to use as a marker for species identification, more reliable mitochondrial and/or nuclear DNA markers need to be developed.