• Title/Summary/Keyword: 유전자재조합

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Monitoring of Genetically Modified Soybean and Processed Foods in Korean Market using PCR (PCR을 이용한 국내시장에 유통중인 유전자재조합 콩 및 가공식품의 모니터링)

  • Kim, Myo-Young;Kim, Jae-Hwan;Kim, Hyun-Joong;Park, Sun-Hee;Woo, Geon-Jo;Kim, Hae-Yeong
    • Applied Biological Chemistry
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    • v.46 no.4
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    • pp.344-347
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    • 2003

  • A method using PCR was developed for the monitoring of genetically modified soybean (GMS) and GMS derived foods utilized in the market. We designed 3 pairs of specific oligonucleotide primers based on epsps and pat inserted in GMS and ferritin gene as internal standards. Template DNAs isolated from soybean and processed foods were used for multiplex PCR with 3 primer sets. PCR, used with specific primer sets for GMS detection, showed the amplified DNA fragments with GMS template DNA. In this study, GMS containing epsps was detected from soy processed foods manufactured before GM food labeling system, however, GMS containing epsps or pat was not detected from soy processed foods manufactured after GM food labeling system.

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