• Title/Summary/Keyword: 유전육종

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Development of New Hybrid Zoysiagrass Cultivar 'Seah' (한국잔디 신품종 '세아(Seah)' 개발)

  • Choi, Joon-Soo;Yang, Geun-Mo;Bae, Eun-Ji;Park, Yong-Bae;Lee, Kwang-Soo
    • Weed & Turfgrass Science
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    • v.6 no.4
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    • pp.306-312
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    • 2017
  • This study was carried out to develop new hybrid zoysiagrass cultivar 'Seah' (The application no. for cultivar protection : 2014-22). Native zoysiagrasses were collected from south-west seaside of Korea from 2010 to 2011. Artificial crossing was conducted to develop F1 hybrid between Z2011 (Z. sinica) and NM1 (Z. matrella) at plastic house in 2011. Among the progenies, 'Seah' showed fine leaf texture and high shoot density from the space planting plots at field. 'Seah' showed genetically light green color, with fine leaf with 1.8mm and height to the lowest leaf blade was 1.94 cm. Ground coverage rate was slower than medium leaf zoysiagrass (Jung-gi), but plant height of 7.1 cm was the lowest among the compared zoysiagrasses and height to lowest leaf of 1.94 cm was lower than most zoysiagrass, which may allow low mowing height.

Classification and Selection of the Breeding materials in the Silkworm, Bombyx mori, by Multivariate Analysis 1. Classification of the Silkworm Genetic Stocks by Principal Component Analysis and Cluster Analysis (다변량 해석법에 의한 누에 육종소재의 탐색 1. 주성분분석과 집락분석을 이용한 누에품종분류)

  • 정도섭;이인정
    • Journal of Sericultural and Entomological Science
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    • v.31 no.2
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    • pp.102-112
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    • 1989
  • Principal component analysis and cluster analysis were performed on the nine quantitative characters of the one hundred and forty eight silkworm genetic stocks. The six major quantitative characters such as cocoon yield, cocoon weight, cocoon shell weight, cocoon shell percentage, larval period of the 5th instar silkworm, and total larval period showed significantly positive correlation between them. The first three principal components extracted form the initial nine variables by principal component analysis accounted for about eighty percent of original information. The first and second principal components were characterized as factors related to silk productivity, and cocoon productivity, respectively. On the basis of multivariate analysis using city block distance determined from the first three principal components to measure the phenotypic diversity, the one hundred and forty eight silkworm genetic stocks could be clustered into seven varietal groups, and the phenotypic diversity between the varietal groups was partly related to their geographical origins. Among 7 varietal group, group II and IV revealed higher silk and cocoon productivity.

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Accumulating Pattern of ${\alpha}-glycosidase$ Inhibitor in Various Silkworm Varities (누에품종별 혈당강하물질 축적양상 구명)

  • Kang, Pil-Don;Kim, Jin-Won;Sohn, Bong-Hee;Kim, Kee-Young;Jung, I-Yoen;Kim, Mi-Ja;Ryu, Kang-Sun
    • Journal of Sericultural and Entomological Science
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    • v.48 no.1
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    • pp.25-27
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    • 2006
  • [ ${\alpha}-Glycosidase$ ] inhibitors slows the velocity of the uptake of monosaccharides in the small intestine by retarding the speed of degradation of disaccharides to monosaccharides, which made it possible to develop ${\alpha}-glycosidase$ inhibitors as the antihyperglycemic ('antihyperglycemic' means 'blood-glucose-level-lowering') reagent for the diabetic patients such as acarbose and miglitol. Twenty kinds of ${\alpha}-glycosidase$ inhibitors have been reported to exist in mulberry, Morns alba, and some of them are also found in the silkworm, Bombyx mari, as the result of its daily feeding of mulberry leaves as the sole diet. 1-Deoxynojirimycin (DNJ), one of the most potent ${\alpha}-glycosidase$ inhibitor, is the most abundant among polyhydroxylated alkaloids with ${\alpha}-glycosidase$ inhibiting activity in both M alba and B. mari, therefore considered the antihyperglycemic criterion of the mulberry- or silkworm-based neutroceutical products. DNJ is thought to be accumulated in the body of silkworm because the its concentration in the silkworm body is two to three times as much as that in the mulberry leaves. Eighteen silkworm F1 varieties have been recommended for industrial rearing in Korea by some standards such as pathological strength. DNJ concentration in 18 recommendedwere measured at 3rd day in the 5th instar after lyophilization to determine the varieties that accumulates DNJ in its body most. GeumOk-Jam was the highest in the DNJ concentration of 5.45 mg/gDW among the recommended F1 varieties.

Shorten heading date of M. sacchariflorus and M. sinensis and single crossing method to breed Miscanthus × giganteus cultivar for bioenergy production (바이오에너지용 이질3배체 억새(Miscanthus × giganteus) 품종육성을 위한 물억새(M. sacchariflorus)와 참억새(M. sinensis) 출수기 단축과 단교배 방법)

  • Moon, Youn-Ho;Kim, Kwang-Soo;Cha, Young-Lok;Lee, Ji-Eun;Kwon, Da-Eun;Kang, Yong-Ku
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.51-51
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    • 2018
  • 본 연구는 바이오에너지용 이질3배체 억새(Miscanthus ${\times}$ giganteus) 품종육성 위한 교배재료인 물억새(M. sacchariflrous)와 참억새(M. sinensis) 출수기 단축과 단교배 방법을 개발하기 위해 수행하였다. 물억새와 참억새를 12시간 일장, 자연일장 조건에서 재배하여 출수기 단축에 미치는 단일효과를 조사하였다. 출수기에 일중 화분 발아시간, 절단한 화분친 이삭 활력 유지 및 격리방법 등 단교배 방법을 구명하여 이를 활용한 억새의 자가 수정 여부를 조사하고 시험교배를 실시하였다. 참억새와 물억새 모두 12시간 일장의 단일 조건에서 재배한 것이 자연일장에 재배한 것에 비해 출수 소요일수가 18~27일 정도 단축되었다. 화분 발아는 물억새에서 오전 6시에 왕성하였으나 시간이 경과할수록 발아율이 낮아져 오전 8시에는 10% 이하만 발아하였다. 참억새 화분은 오전 6시에는 발아하지 않았으나 오전 7시에 50% 이상이 발아하였고 8시에는 물억새와 같이 10% 이하였다. 화분친 참억새 이삭을 절단, 절화 보존액에 꽂아 백색 부직포로 격리하였을 때 절화 보존액량이 많을수록 활력 유지 일수가 증가하여 150 mL에서 물억새, 참억새 모두 7일간 개화 및 화분 비산을 지속하였다. 이 때 화분 발아율은 참억새와 물억새 모두 4일까지 40%를 유지하였다. 참억새와 물억새는 자가수정율이 ~ 2.5%로 낮고, 자연교잡 임실율은 출수기가 빠른 유전자원에서 54.4%까지 높았다. 상기 단교잡 방법을 적용한 물억새 4배체와 참억새 2배체간 14조합 시험교배로 437립의 종자를 얻었다. 본 연구의 억새 종간 단교배 방법은 우수한 종자친과 화분친으로 교배종자를 얻을 수 있어 향후 바이오매스 수량이 많으면서, 종자가 맺히지 않은 이질 3배체 품종육성에 활용할 수 있을 것으로 판단된다.

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Development of high tryptophan GM rice and its transcriptome analysis (고 함량 트립토판 생산 GM 벼 개발 및 전사체 분석)

  • Jung, Yu Jin;Nogoy, Franz Marielle;Cho, Yong-Gu;Kang, Kwon Kyoo
    • Journal of Plant Biotechnology
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    • v.42 no.3
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    • pp.186-195
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    • 2015
  • Anthranilate synthase (AS) is a key enzyme in the biosynthesis of tryptophan (Trp), which is the precursor of bioactive metabolites like indole-3-acetic acid and other indole alkaloids. Alpha anthranilate synthase 2 (OsASA2) plays a critical role in the feedback inhibition of tryptophan biosynthesis. In this study, two vectors with single (F124V) and double (S126F/L530D) point mutations of the OsASA2 gene for feedback-insensitive ${\alpha}$ subunit of rice anthranilate synthase were constructed and transformed into wildtype Dongjinbyeo by Agrobacterium-mediated transformation. Transgenic single and double mutant lines were selected as a single copy using TaqMan PCR utilized nos gene probe. To select intergenic lines, the flanking sequence of RB or LB was digested with a BfaI enzyme. Four intergenic lines were selected using a flanking sequence tagged (FST) analysis. Expression in rice (Oryza sativa L.) of the transgenes resulted in the accumulation of tryptophan (Trp), indole-3-acetonitrile (IAN), and indole-3-acetic acid (IAA) in leaves and tryptophan content as a free amino acid in seeds also increased up to 30 times relative to the wildtype. Two homozygous event lines, S-TG1 and D-TG1, were selected for characterization of agronomic traits and metabolite profiling of seeds. Differentially expressed genes (DEGs), related to ion transfer and nutrient supply, were upregulated and DEGs related to co-enzymes that work as functional genes were down regulated. These results suggest that two homozygous event lines may prove effective for the breeding of crops with an increased level of free tryptophan content.

High-efficiency and Rapid Agrobacterium-mediated genetic transformation method using germinating rice seeds (벼 발아초기 종자를 이용한 고효율 단기형질전환 방법)

  • Lee, Hye-Jung;Abdula, Sailila E.;Jee, Moo-Geun;Jang, Dae-Won;Cho, Yong-Gu
    • Journal of Plant Biotechnology
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    • v.38 no.4
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    • pp.251-257
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    • 2011
  • Rice is the most important crop as a model plant for functional genomics of monocotyledons. Rice is usually transformed using Agrobacterium tumefaciens. However, the transformation efficiency using previous method is still low. In this study, we established a new method by modifying the general Agrobacterium protocol especially in the inoculation and co-cultivation step. We directly inoculated Agrobacterium containing a CIPK15 gene under the control of CaMV 35S promoter and NOS terminator in the pCAM1300 vector into the pre-soaked seeds in N6D media for 24 hours. After 7 days of culture at $25^{\circ}C$, calli were formed on seeds cultured on the co-cultivation medium containing an antioxidant compound (1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (3 mg/L silver nitrate). We obtained 35 and 22 transgenic plants in rice cultivars, Gopumbyeo and Ilpumbyeo, with increase of transformation efficiency by 30.4% and 22.6%, respectively compared to the general transformation method. The new method in this study would lead to reduction of substantial labor and time to generate transgenic plants.

Low temperature and Salt Tolerances of Native Zoysiagrass (Zoysia spp.) Collected in South Korea (국내 자생 한국잔디류의 내한성 및 내염성 조사)

  • Choi, Joon-Soo;Yang, Geun-Mo
    • Asian Journal of Turfgrass Science
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    • v.25 no.2
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    • pp.138-146
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    • 2011
  • This study was carried out to select salt tolerant zoysiagrass breeding lines. Eighty two native zoysiagrasses collected from S. Korea were used in this study. Saline water were prepared by mixing sea water and tap water. ECw levels of saline water treated ranged from 2 to $3dS{\cdot}m^{-1}$. Zoysiagrass planted in pot by sprigging were soaked into the plastic box containing saline water. Winter injury was investigated under the pot condition. Most of Z. japonica types did not show winter injury. But Z. tenuifolia type, Z. matrella type, and Z. sinica type showed winter injury under the pot condition at Cheonan area. NaCl level in soil was increased from 0% to 0.51% by treatment of saline water. Soil ECe measurement showed upto $170dS{\cdot}m^{-1}$. Z. tenuifolia type (Z5034), Z. matrella type ('Konhee', Z4109, 'Semill'), Z. japonica type (Z1055, Z1040, Z1008, 'Zenith', 'Millock') and medium leaf type zoysiagrass (Z6096, Z6118, Z6021, Z6074) resulted in below 30% leaf firing under the saline condition. This approach might be useful for selecting salt tolerant breeding lines.

Genetic and of environmental effects for economic traits in pigs (돼지 주요 경제형질들에 영향을 미치는 유전 및 환경효과 추정)

  • Lee, Ill-Joo;Hong, Joon-Ki;Kim, Du-Wan;Sa, Soo-Jin;Kim, Young-Hwa;Cho, Kyu-Ho
    • Korean Journal of Agricultural Science
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    • v.40 no.4
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    • pp.347-351
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    • 2013
  • This study was conducted to estimate the effect of sex, breed and on the basis of the records on 300 pigs of Duroc, Landrace and Yorkshire breeds raised at the National Institute of Animal Science. Yorkshire was observed to be superior to other breeds between 90 to 110 kg of body weight. Thicker backfat was estimated in the B-mode than in A-mode of 110 kgs range than in 90 kgs. Loin eye muscle area was larger in the final weight of 110kg than in 90 and the Duroc had significantly (p<0.05) larger loin eye muscle area than other breeds. Thinner backfat was observed in males than in females in both modes of backfat measurements and in both of the final weights of test. Females had significantly larger loin eye muscle area than males, and the difference between the two sexes were larger in the final weight of 110 kg than in 90 kg. Analysis models concluded that backfat thickness certainly influences the body weight gain when at 90 kgs and 110 kgs analysed with A-mode and B-mode respectively.

The Production, Distribution, and Utilization of Rice in Japan (일본의 쌀 생산, 유통 및 이용 현용)

  • Inouchi, Naoyoshi
    • Food preservation and processing industry
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    • v.1 no.1
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    • pp.17-26
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    • 2002
  • 일본은 기후적으로도 쌀의 생산에 적합하며 쌀은 일본의 주곡이다. 1893년 국립농업연구소가 설치된 이래로 쌀은 품질과 단보당 생산량이 꾸준히 개선되어와 116의 노동시간으로 생산량은 2.5배의 증가를 가져왔다. 이러한 진전은 재배방법의 개선, 비료, 농약, 재배기술, 기계화, 관개배수, 농지개량 등의 요인들로 볼 수 있다. 현재 일본에서 생산되고 있는 주요 쌀 품종으로는 코시히카리($35.5\%$), 히토메보레($9.7\%$), 히노히카리($9.0\%$), 아키다코마치($8.5\%$), 키라라($4.8\%$) 등 10여 품종이며 최근에는 형질전환 품종의 연구와 함께 생물공학적 연구 ,생리, 품질, 곤충과 잡초, 토양, 식품가공 등 기본적인 연구에 심혈을 기울이고 있다. 새로운 형태의 쌀 품종으로는 냉동조리쌀밥, 초밥, 레토르트룡 쌀 등 가공용으로 적합하도록 하기 위하여 아밀로오즈의 함량을 $5-15\%$로 낮춘 쌀이나 카레, 필라프, 튀김 쌀 등의 용도에 적합하도록 아밀로오스의 함량을 $30\%$이상으로 높인 쌀뿐만 아니라 곡립의 크기와 길이 등을 변형시킨 쌀, 유색미, 향미, 단백질 함량 조절 쌀, 거대 배아미, lipoxygenase 활성을 없앤 쌀, 단맛나는 쌀 등이 연구, 생산되고 있다. 일본에서의 쌀이용은 먼저 가공용 쌀을 들 수 있다. 밥, 청주, 스낵, 쌀가루, 미소발효 등 다양하게 이용이 되고 있으며 parboiled rice와 이의 색과 향을 개선한 converted rice, 현미를 이용한 가공쌀, 쌀빵, 현미가루, 세척미, 쌀국수, 쌀스낵, 당과, 죽, 쌀은 채소아 함께하는 타키코미고한, 초밥, 냉동쌀밥, 무균포장밥, 건조밥, 즉석밥 등 매우 다양하다.는 일, 쌀 재배구조 조정과 함께 높은 미질을 가지는 품종육종, 기계화를 비롯한 경작기술의 발달, 쌀과 부산물 가공기술의 개발연구, 특정기능을 함유하는 유전공학적 기술의 적용, 토지와 도시화 그리고 식량순환에 시스템의 개혁 등 과학기술을 고양하는 일 등을 들 수 있다.하는데 도움이 되리라 생각된다.=0.002)가 통계적으로 유의한 인자였다. 결론 : 본 기관에서 시행되어진 근침윤성 방광암에 대한 방광보존치료법은 기존의 근치적 방광절제술에 비하여 대등한 치료성적을 내는 동시에 $63\%$에서 장기보존이 가능하였다. 따라서 본 치료법이 방광암의 치료에 적극적으로 적용되어야 할 것으로 생각하며 향후 여러 기관이 참여하는 활발한 연구를 통해 한국인에게 가장 적절한 치료법을 개발해야 될 것으로 생각한다. B2+3, C1, C2+3군에서 수술달독군과 방사선치료 추가군의 골반종양제어율은 각각 $79\%$$75\%$ (p=0.88), $100\%$$100\%,\;44\%$$68\%$ (p=0.01)이었다. 전체 환자를 대상으로 다요인 분석을 시행한 결과 생존율과 무병생존율에 병기만이 유의하였고 두 치료 군에서도 역시 병기가 유의한 인자로 나타났다. 전체환자에서 골반종양제어율에 유의한 예후인자로 다요인분석을 시행한 결과 병기와 수술방법이 유의하였다. 수술단독군에서는 병기만이 유의하였고 방사선치료 추가군에서는 수술방법만이 유의하여 복부회음절제술군의 골반종양재발률이 높았다. 결론 : 본 후향적 연구에서 수술 후 보조적 방사선치료를 시행하여 수술단독군에 비해 병기 C2+3군에서 골반종양제어율이 향상되었음을 알 수 있었다. 그러나 병기 B2이상의 모든 환자에서 골반종양제어율 뿐만 아니라 생존율의 향상을 가져오기

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Varietal Differences of Pod Shedding (땅콩 낙협 정도의 품종간차이)

  • 진일두;김규태
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.6
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    • pp.532-539
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    • 1991
  • This study was conducted to screen genetic stock for resistant to pod shedding. Materials used were 7 recommended varieties in Korea and 211 introduced varieties containing 94 Virginia type, 99 Spanish type and 18 Valencia type varieties. the days from seeding to first anthesis. number of mature pods per plant. 100 seeds weight and shedded pod ratio per total mature pod at harvesting were investigated. And the breaking tensile strength required to detach a pod from its gynophore was measured at a proper time for harvesting of each variety. In addition. factors related to pod shedding were discussed. The first anthesis of Spanish and Valencia types were earlier than that of Virginia type, but Korean recommended varieties which were recognized as Virginia type had relatively earlier anthesis. The varieties of Spanish type yielded more pods compared with those of Valencia and Virginia types. but Korean recom-mended varieties yielded slightly more pods than the others. Seeds weight of Korean recommended varieties was larger than not merely those of Spanish and Valencia types which were small seeds but also those of Virginia type which were large seeds. The breaking tensile strengths between pod and gynophore were various among the varieties belonging to same type. The strength was relatively strong in the Spanish type, but weak in both Virginia type and Korean recommended varieties. Therefore, pod shedding in the varieties of Spanish type was low and high in Virginia type and Korean recommended varieties. There were positive or negative correlations at the 0.1 % significan level among the days to first anthesis. number of pods per plant. seed weight, breaking tensile strength and shedding ratio. The pod shedding is considered a compound character that is affected by the factors such as breaking tensile strength between pod and gynophore, number of pods per plant and seed size.

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