• Journal of Mushroom
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• v.5 no.1
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• pp.34-38
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• 2007

The white-colored and the dark gray-colored mutants were frequently happened in cultivated areas of Pleurotus ostreatus (Wonhyeong-neutari). These caused conflicts between farmers and spawn companies. Our studies were conducted to elucidate the mechanism of mutagenesis. The results from the studies would provide valuable informations that could be used to prevent the color-related mutation, and also will be applied in breeding programs of P. ostreatus. Oyster mushroom variety, Wonhyeong-neutari, is somatic hybrid of Pleurotus and has genetic makers for arginine, ornithine, proline, riboflavine. Genetic markers analysis of monospore isolates derived from color mutants show identical tendency with that of Wonhyeong-neutari, these results indicate that color mutants were derived from Wonhyeongneutari. Twenty-one and four homokaryons were selected from the white-colored mutant MGL 2205 and gray-colored ASI 2029. All 34 F1 hybrids derived from the white-colored mutant MGL 2205 produce white-color fruiting bodies, indicating that the white color trait is heritable. In the first generation hybrids between the white-colored MGL 2205 and the gray-colored ASI 2029, all 16 hybrids produced pigmented fruiting bodies. Homokaryons isolated from the hybrid MGL 2205 X ASI 2029 were mated with homokaryon tester strains derived from MGL 2205. By these result, we could assumed that white color trait is a heritable character which is controlled by more than one recessive gene.

• Research in Plant Disease
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• v.20 no.4
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• pp.253-258
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• 2014

This study was conducted to evaluate tomato plant resistance against bacterial wilt by Ralstonia solanacearum using tomato cultivars or tomato breeding lines maintained in RDA-Genebank of Rural Development Administration and to select resistant tomato lines for breeding purpose. We evaluated the disease responses of a total of 13 cultivars and 39 breeding lines from RDA-Genebank using R. solanacearum SL341 strain, which is a representative strain in Korea. Tomato cultivar Hawaii 7996 and Moneymaker were used as a resistant control plant and a susceptible control plant, respectively. A total of 32 cultivars were susceptible and 10 cultivars showed various disease response suggesting resistant phenotype segregation in the lines. Five commercial cultivars and 5 breeding lines exhibited strong resistance to bacterial wilt by the SL341 strain. These 5 breeding lines might be used for further study of plant defense response against bacterial wilt and cloning of the resistance gene from tomato plants. Ultimately, the selected lines could be used for tomato breeding to generate bacterial wilt resistant tomato plants.

• Current Research on Agriculture and Life Sciences
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• v.12
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• pp.23-28
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• 1994

A leading sweet pepper cultivar, Keystone Resistant Giant #3, was crossed with a line with resistance to Xanthomonas campestris pv. vesicatoria, PI271322, for incorporation of the resistance and to study the inheritance of resistance to the disease. Seedlings of parents, $F_1$, $F_2$ and backcross populations of a cross between Keystone Resistant Giant #3 and PI271322 were inoculated with race 1 and race 3 of Xanthamonas campestris pv. vesicatoria by infiltrating bacterial suspension into abaxial side of leaves. PI271322 was carrying a gene ($Bs_3$) for hypersensitive resistance to race 1 of X. c. pv. vesicatoria and also a component of non-hypersensitive resistance to race 3 inheriting in a quantitative mode. Correlation coefficient between disease indices to race 1 and race 3 of non-hypersensitive plants in $F_2$ population was highly significant, thus indicating that a component of non-hypersensitive general resistance in PI271322 acted consistently to both races. Individual plants with resistance to both race 1 and race 3 of X. c. pv. vesicatoria were selected and a breeding program for incorporation of the resistance is continued.

• Horticultural Science & Technology
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• v.32 no.4
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• pp.525-534
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• 2014

Microsatellite markers were used to identify 58 major commercial melon cultivars, and to assess hybrid seed purity of a melon breeding line known as '10H08'. A set of 412 microsatellite primer pairs were utilized for fingerprinting of the melon cultivars. Twenty-nine markers showed hyper-variability and could discriminate all cultivars on the basis of marker genotypes, representing the genetic variation within varietal groups. Cluster analysis based on Jaccard's distance coefficients using the UPGMA algorithm categorized 2 major groups, which were in accordance to morphological traits. The DNA bulks of female and male parents of breeding line '10H08' were tested with 29 primer pairs based on microsatellites to investigate purity testing of $F_1$ hybrid seeds, and 5 primer pairs exhibited polymorphism. One microsatellite primer pair (CMGAN12) produced unambiguous polymorphic bands among the parents. Among 192 seeds tested with CMGAN12, progeny possibly generated by self-pollination of the female parent were clearly distinguished from the hybrid progeny. These markers will be useful for fingerprinting melon cultivars and can help private seed companies to improve melon seed purity.

• Journal of Animal Science and Technology
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• v.48 no.2
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• pp.143-150
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• 2006

The data were collected in the dairy herd improvement program from January 2000 to July 2005. Test data included 825,157 records of first parity and animals with both parents known were included. This study aimed to describe the effect of incomplete lactation records of latest generation to the change in sire's breeding value using Random Regression model (RRM) in genetic evaluation. Estimation of genetic parameter and breeding value for sire used REMLF90 and BLUPF90 program. The phenotypic value on the number of test day records between group TD11, TD8, TD5, TD2 showed no large differences. For all the group heritability of test day milk yield range from 0.30 to 0.36. However TD2 group showed low heritability the least test day recode on the latest generation. The correlation of above 50% between test day and TD11(0.610), TD8(0.616), TD5(0.661) and TD2(0.682) with different records in latest generation. Sire's rank of breeding value varied widely depending on the records on the number of lactation from start to the latest generation. Study showed that change in breeding value ranked if daughter's test recode more so it should have at least 5 test day records. The use of RRM in dairy cattle genetic evaluation would be desirable if complete lactation records for latest generation daughters of young bulls when selection for proven bulls. Random Regression model (RRM) require at least 5 test-day lactation recode.

• Horticultural Science & Technology
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• v.28 no.5
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• pp.802-809
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• 2010

Phytophthora blight of pepper is the most economically important disease in the world cultivation regions. We investigated the phytophthora blight resistance of 300 accessions of Korean landrace of hot pepper germplasms collected from 83 local regions. The disease incidence rate was checked from 7 days to 28 days at an interval of 7 days after inoculation under greenhouse conditions. Among 300 accessions, the disease incidence rate of phytophthora blight of 67 accessions of pepper germplasm was more than 60.1%, while no disease was observed in 37 accessions at 7 days after inoculation. At 28 days after inoculation, five and eleven accessions of pepper germplasm were resistance and moderate resistance to $P.$ $capsici$, respectively. Two hundred forty four susceptible accessions (81.3%) of pepper were scored as having more than 60.1% of disease incidence of phytophthora blight. This result suggests that five candidate pepper germplasm might be used as breeding resources for the phytophthora blight resistance breeding program. Also, further genetic studies should be carried out to verify this result, with the overall focus of providing information on important characteristics of pepper germplasm.

• Korean Journal of Plant Resources
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• v.28 no.5
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• pp.648-655
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• 2015

Balloon flower (Platycodon grandiflorum A. DC.) is a perennial plant of mainly Campanulaceae family, which have been widely used as a food ingredient and herbal medicine in East Asia. Although demands on related products and yearly cultivation area for balloon flower are increasing, diverse fundamental technologies and molecular breeding studies are not very well supported in Platycodons. In this study, 30 random amplification of polymorphic DNA (RAPD) primers were test in an attempt to explore genetic diversities. In addition, sequences information of the actin gene, a well conserved gene encoding a globular protein that forms microfilaments, was retrieved and analyzed. Two actin homologs were recovered; 3.4 kb fragment is a Pg-actin and 1.4 kb fragment is a Pg-actin homolog with 28.6% similarity. We have confirmed that the Pg-actin gene is configured into 4 exons and 3 introns. A single nucleotide polymorphism (SNP), G↔A, was detected on the intron 3, which served as a target for the CAPS marker development. The marker Pg-Actin-Int3 was applied to 32 balloon flower accessions. Balloon flower DNA sequence information generated in this study is expected to contribute to the analysis and molecular breeding and genetic diversity analysis of balloon flowers.

• Journal of Plant Biotechnology
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• v.33 no.2
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• pp.85-91
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• 2006

Flavanone 3$\beta$-hydroxylase (FHT) is an enzyme acting in the central part of the flavonoid biosynthesis pathway. FHT catalyses the hydroxylation of flavanone to dihydroflavonols in the anthocyanin pathway. In this paper we describe the cloning and expression of the genes encoding the flavonoid-biosynthetic enzyme FHT in Gypsophila paniculata L. A heterologous cDHA probe from Dianthus cavophyllus was used to isolate FHT-encoding cDHA clones from Gypsophila paniculata L.. Inspection of the 1471 bp long sequence revealed an open reading frame 1047 bp, including a 190 bp 5' leader region and 288 bp 3' untranslated region. Comparison of the coding region of this FHT cDHA sequence including the sequences of Arabidopsis thaliana, Citrus sinensis, Dianthus caryophyllus, Ipomoea batatas, Matthiola incana, Nierembergia sp, Petunia hybrida, Solanum tuberosum, Vitis vinifera reveals a identity higher than 69% at the nucleotide level. The function of this nucleotide sequences were verified by comparison with amino acid sequences of the amino-terminus and tryptic peptides from purified plant enzyme, by northern blotting with mRHA from wild type and mutant plants, by in vitro expression yielding and enzymatically active hydroxylase, as indicated by the small dihydrokaempferol peak. Genomic southern blot analysis showed the presence of only one gene for FHT in Gypsophila paniculata.

• Current Research on Agriculture and Life Sciences
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• v.4
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• pp.12-17
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• 1986

Six genetic populations, viz, $P_1$, $P_2$, $F_1$, $F_2$, $B_1$, and $B_2$ of three crosses involving five varieties ($342{\times}$Kimjanggochu, Masan${\times}$Hungarian was, and Masan${\times}$Jeju) were used for estimating heterosis, heterobeltiosis, inbreeding depression, genetic components, heritability and correlations for fresh and dry anatomical components of red pepper. The results obtained were summarized as follows. Heterosis was positive in most characters of three crosses and heterobeltiosis was negative except in cross $342{\times}$Kimjanggochu. Inbreeding depression was positive, showing decrease of weight in $F_2$ generation. Duplicate and complimentary type of epistasis were concerned with the expression of characters, and broad and narrow sense heritabilities of fruit weight and pericarp weight were fairly high and stem weight, placenta weight and seed weight were low. Correlations among the anatomical components were positively significant in fresh and dry weights.

• Journal of agriculture & life science
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• v.44 no.5
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• pp.29-33
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• 2010

Soybean (Glycine max (L.) Merr.) seed is the main source of protein and oil for human and animal. The use of soybean protein has been expanded in the food industry due to their excellent nutritional benefits. But, antinutritional and allergenic factors are exist in the raw mature soybean. Lipoxygenase, Kunitz trypsin inhibitor (KTI) protein, and ${\alpha}^{\prime}-subunit$ of 7S globulin are main antinutritional factors in soybean seed. Breeding of a new soybean strain with lacking these components is needed. The objective of this research was to select new soybean line with lipoxygenase-free, KTI-free, and ${\alpha}^{\prime}-subunit$ free (lx1lx1lx2lx2lx3lx3titicgy1cgy1 genotype). Total 434 $F_2$seeds were obtained from the cross of cultivar, "Gaechuck#2" and PI506876. Presence and absence of lipoxygenase, KTI protein, and ${\alpha}^{\prime}-subunit$ of 7S globulin was tested by SDS electrophoresis using a partial seed of each $F_2$seed. Only one $F_2$seed with lacking these three components was selected and was planted to $F_2$plant. Absence of lipoxygenase, KTI, and ${\alpha}^{\prime}-subunit$ protein was confirmed on the $F_3$seeds harvested. Selected line with lx1lx1lx2lx2lx3lx3titicgy1cgy1 genotype might be useful for soybean breeding.