• Korean Journal of Environment and Ecology
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• v.14 no.3
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• pp.205-211
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• 2000

본연구는 조경소재로 이용가능성이 크지만 현재 자생지가 파괴되어 복원이 요구되고 있는 야생자란의 대량번식을 위해 무균배양시 배지 내 담함량의 변화와 펩톤, 트립톤 등의 첨가가 종자발아와 계대배양 후 유식물의 생육에 미치는 영향을 알아보고자 실시하였다. 배지 내 펩톤과 트립톤의 첨가는 발아에 영향을 주지는 않았지만, 당의 함량은 그 농도가 10g/L까지 증가함에 따라 발아율을 높였다. 또한 발아 후 유식물의 생육시 당의 첨가는 뿌리의 생육을 두드러지게 향상시켰으며, 생체중도 거의 2~3배정도 많았다. 하이포넥스 배지(대조구)에서는 높은 발아율을 보였지만 유식물의 생육은 트립톤 첨가배지(2g/L)에서 많았는데 엽수, 뿌리수, 엽장 근장, 생체중 등이 모두 다른 처리구의 2~3배에 이르는 초기생육을 보였다. 계대배양 이후의 생육상은 펩톤 첨가배지에서 가장 많은 생육량을 보였는데 특히 엽장과 엽폭 그리고 근장이 다른 처리구보다 월등히 높은 경향을 나타냈다. 생체중도 한 개체당 0.18g으로 가장 높게 나타나 펩톤의 첨가가 계대배양 이후의 생육을 크게 촉지시키는 것으로 나타났다. 결론적으로 하이포넥스 배지에 트립톤 2g/L를 첨가하였을 때 발아율과 유식물의 생육이 다른 배지에 대해 매우 양호한 것으로 나타나 자생 자란의 종자발아용 배지로 가장 적당한 것으로 사료된다. 그리고 이후 계대배양시에는 펩톤의 첨가 (3g/L)가 유식물의 생육을 가장 촉진시키는 것으로 나타났다.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.411-419
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• 2003

The potent biosynthetic precursors, 24$\alpha$-methylcholesterol and 24$\alpha$-methylcholestanol, and the endogenous brassinosteroids (BRs), castasterone (CS) and 6-deoxocastasterone (6-deoxoCS), were identified from shoots of maize seedlings. In addition, the presence for activities of several enzymes involved in the late C6-oxida-lion pathway from 24$\alpha$-methylcholestanol to CS was demonstrated in the plants. However, activity for brassinolide (BL) synthase which catalyze the conversion of CS to BL, the last step of the late C6-oxidation pathway, was not detected in the enzyme solution obtained from the maize shoots. Together with the fact that BL was not identified from the maize shoots, these results strongly suggested that BRs in the maize shoots are biosynthesized during seedling growth and the active BR in the shoots is not BL but CS.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.1
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• pp.19-23
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• 1995

Cotyledon and leaf development of six important forage legums were compared to study their contribution to the seedling growth, Cotyledons of forage legumes expanded their size rapidly during one week maximum size was reached and entered senescence. Larger seeds produced greater colyledon area, and speed of colyledon expansion was closely associated with seedling growth. Earlier onset leaf production and earlier leaf development were major determinant factor initial seedling growth. Alfalfa and red clover which have larger seeds were good in seedling vigor with larger photosynthetic area. Alsike clover and white clover with small seeds showed also good seedling vigor, as they developed leaves early with relatively high photosynthetic rate. On the other hand, though lespedeza has heavy seeds, its seedling vigor was very poor with slow photosynthetic area development and low photosynthetic rate.

• Korean Journal of Environmental Biology
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• v.26 no.4
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• pp.349-354
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• 2008

Superoxide dismutase (SOD) activities were investigated from the portions of shoots and roots in accordance with developmental stage and in response to environmental stress and antioxidants using Rhodiola-seedlings. The rates of SOD activities were revealed highly at the portion of roots and its tip of seedlings in the latter stages rather than the initial stages. SOD activities of seedlings in the initial stages treated with sodium chloride and cadmium as environmental stressors showed the decrease by 15 and 30% with respect to the control, respectively. However, in spite of stressor-treatments, the activities in the roots were increased according to the growth period showing a maximum rate of up to 45%. Also, SOD activities of the seedling treated with ascorbic acid as a antioxidant were increased by 46% of control value, but this was similar to the rate revealed in the presence of stressors. These results suggest that SOD activities in Rhodiola-seedlings may be related with the important defence-system against injurious environments.

• Korean journal of applied entomology
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• v.52 no.4
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• pp.371-377
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• 2013

Occurrence of adult alfalfa weevil, Hypera postica Gyllenhal, was observed at Chinese milk vetch field, and host plant preference of its $1^{st}$ generation adult was observed at greenhouse and Chinese milk vetch field. The over-wintered adult of the weevil appeared from late March and peaked in mid April while the $1^{st}$ generation adult appeared from late April and peaked in late May. However, the occurrence of over-wintered adult was very low contrary to the $1^{st}$ generation adult which was very high with some variations among observed years. In greenhouse, host plant preference of $1^{st}$ adult generation of H. postica was evaluated with 11 different seedlings. H. postica was found to occur in significantly higher number on Chinese cabbage seedling followed by soybean, and kale at 7 days after releasing(DAR). Corn, crown daisy and sorghum were found to be the least preferred with nil occurrence of the weevil. Seedlings damage rate by H. postica feeding at 7DAR was the most severe on Chinese cabbage(60.0%) followed by soybean(50.0%), kale(30.0%), foxtail millet(16.7%), proso millet(13.3%) and lettuce(3.3%) significantly. Also, seedlings damage rate by H. postica at 7DAR in Chinese milk vetch field had shown the similar trend with Chinese cabbage(46.7%), soybean(43.3%), kale(23.3%), foxtail millet(13.3%) and proso millet(13.3%) in greenhouse significantly. Accordingly, results on host plant preference of H. postica from this study can be used as a basic information for safe cultivation of upland crops at near or around green manure crop field.

• Journal of Environmental Science International
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• v.5 no.5
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• pp.643-656
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• 1996

Various light intensity and light quality were treated to oat seedlings to investigate the effect of light on the chlorophyll accumulation and the formation of chlorophyll-protein complexes. The Increase of total chlorophyll accumulation and Chl (chlorophyll) a/b ratio was promoted under H (high intensity) white light during oat chloroplast development when compared to 1 (low intensity) white light. Also H white light was more effective in the formation of chlorophyll-protein complexes associated with PSI CCI and CCII than L white light. The seedlings grown in various right quality caused little changes in total chiorophyl and ChI-a/b ratio when compared to those grown in L white light. The assembly of LHCII trimer was more affected by L white light treatment in the formation of-chlorophyll-protein complexes than red light treatment. The effect of blue light on the relative composition of chlorophyll-protein complexes was similar to that of L white light. Particualrly, blue light was more effective in the synthesis of LHCII monomer than the other light quality at the early stage of greening. When compared to red light, blue light was more effective the increase of chlorophyll accumulation and Chl a/b ratio than 1ight Quality, and light Quality may be in important factor for the regulation of the organization in the chlorophyll-protein complexes curing greening.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.4
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• pp.288-294
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• 1994

Seedling vigor of introgressive hybrids between birdsfoot refoil 'MO-20"(Lotus corniculatus) used as the maternal parent and tetraploid(2n=4x=24)accession PI302921 narrowleaf trefoil(Lotus corniculatus L.), and parents were compared in terms of dry matter accumulation , net photosynthetic rate, dark respiration rate, and growth analysis. The hybrids showed better seedling vigor than PI302921 as they had lower dark respiration rate and higher relative growth rate (RGR) than PI302921. Hybrids grew faster than MO-20, but there was not significant difference between them. Net photosynthesis per unit area increased during three weeks after emergence without difference among the entries, then decreased sharply with leaf age and shading marking much differences among entries Dark respiration rate was very high just after emergence, after which it decreased rapidly until it reached a stable level at 1 week through 3 weeks of age, and then dropped again. In general, dark respiration, photosynthesis, and seedling growth of the hybrid was similar to those if MO-20. Although there are some reports about interspecific hybrids of Lotus species, it still remains as a new area for genetic improvement of seedling vigor of L. corniculatus.atus.

• Journal of Plant Biology
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• v.37 no.1
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• pp.93-99
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• 1994

은행나무와 곰솔의 유식물의 산성비에 대한 생장에 미치는 영향을 알아보기 위하여 pH 5.6을 대조구로 하여 pH 4.0, 3.2와 2.4의 인공산성비를 3개월간 처리하여 생장량, 엽록소 함량과 탄수화물의 함량을 조사하였다. 은행나무와 곰솔의 유식물은 pH2.4에서 생장이 가장 억제되었으며 반면에 pH 3.2에서는 생장이 촉진되었다. 전체적인 생장량은 뿌리에 비해 지상부의 생장에서 산성비의 영향을 더 받았다. 엽록소 함량은 산성비의 산도에 따라 중요한 차이가 없었으며 은행나무 잎의 탄수화물의 함량은 포도당과 설탕의 함량이 pH 4.0에서 가장 낮았고 전분의 함량은 pH 3.2에서 가장 낮았다.

• Journal of Plant Biology
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• v.34 no.2
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• pp.113-119
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• 1991

The contents of reducing sugar, sucrose, starch and fructose-2,6-bisphosphate (F-Z,$6-P_2$) in relation to the activities of amylase, invertase and fructose-1,6-bisphosphatase (FBPase) were investigated from the leaves of rice (Oryza sativa L. cv. Samjin) seedlings grown at $4^{\circ}C$ for 3 days_ In the seedlings, the contents of reducing sugar and sucrose were increased, but soluble and insoluble starch were declined. Under this condition, amylase activity was increased. but acid invertase activity was declined and alkaline invertase activity was not changed. Cytosolic and stromal FBPase activities were increased. But F-2,$6-P_2$ content was declined. It seemed that the increase of reducing sugar content might be due to the increased activity of amylase and the increase of sucrose content might be related to the increased activity of cytosolic FBPase, reduced content of F-Z,$6-P_2$ and reduced rate of hydrolysis of sucrose during the cold treatment. These results suggested that the changes in carbohydrate rnetabolim of rice seedlings under low temperature reflect one of the protection mechanism to the low temperature during the cold treatment.atment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.1
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• pp.13-18
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• 1995

Seedling development among reciprocal FI hybrids and their parents, Lotus comiculatus and L renuis, were studies using a simple method of measuring cotyledon area. Cotyledon area attached to the plant could be measured with simple rapid method. Cotyledons expanded very rapidly during the first week, then slightly expanded until maximum size was reached around 3 weeks after emergence. Cotyledon area upon emergence was correlated with seed size (P=0.05), but after emergence there was a divergence in cotyledon expansion rate. Rapidity of photosynthetic area development was determinant factor for seedling growth of trefoil species. 2 $\times$ m2 and 1 $\times$ m2 among the hybrids promised the possibility increasing seedling vigor of birdsfoot trefoil.