• Journal of Life Science
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• v.24 no.2
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• pp.154-160
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• 2014

Ghost cells have been recognized as eliciting humoral and cell-mediated immune responses and have also been predicted to play a role as an immune adjuvant. In this study, we used the intramuscular (IM) route to inject BALB/c mice with four vaccine groups constructed from Salmonella typhimurium ghost (STG) cells originating from different virulent strains and complex STG groups instead of heat-labile toxin (LT)-B, a type of adjuvant. Although the complex STG groups exhibited a response after a short delay, the groups showed final total IgG levels similar to those of the LT-B group, which encodes LT-B from pMMP300. The IgG1 response to the ${\chi}$3339 group was the highest response at 6 weeks, whereas IgG2a responses to the ${\chi}$3339 and JOL389 groups were higher at 6 and 8 weeks compared to those of the LT-B group. The response of vaginal sIgA to the LT-B group was generally higher than that of the other groups, whereas fecal sIgA to the LT-B group exhibited lower responses. Protection to virulent S. typhimurium in all groups was above 80%, which was similar to the LT-B group. Taken together, we suggest that STG complex groups can be used as an immune adjuvant instead of LT-B.

• Applied Biological Chemistry
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• v.36 no.6
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• pp.517-524
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• 1993

To characterize glutelins, the most abundant storage protein in rice, 13 complete coding sequences of glutelin genes from the database were analyzed. According to the phylogenic analysis, these genes could be classified into 5 groups, Group I to V. The degrees of homology were calculated to be in the range of 90 to 60%, but the patterns of hydrophobicity were similar in all the groups. Also, each group was found to have similar amino acid composition with variations in lysine content from 2.5 to 3.6% due to the point mutation of arginine to lysine. The isoelectric points of mature proteins and their basic chains of all the groups showed the value of about 9.0 and 10.0, respectively, while the isoelectric points of acidic chains in these groups showed the distinct value of 6.6, 6.7, 7.2, 8.4 and 7.9. The plot of the fraction of G+C at synonimous site in codons (GC3s) against effective codon numbers suggest no major difference in translational efficiency in the expression of glutelin multigenes.

• The Journal of Society for e-Business Studies
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• v.18 no.2
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• pp.95-110
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• 2013

This paper presents a novel feature engineering technique that can improve the conventional machine learning-based text classification systems. The proposed method extends the initial set of features by using hyperlink relationships in order to effectively categorize hypertext web documents. Web documents are connected to each other through hyperlinks, and in many cases hyperlinks exist among highly related documents. Such hyperlink relationships can be used to enhance the quality of features which consist of classification models. The basic idea of the proposed method is to generate a sort of ed concept feature which consists of a few raw feature words; for this, the method computes the semantic similarity between a target document and its neighbor documents by utilizing hierarchical relationships in the WordNet ontology. In developing classification models, the ed concept features are equated with other raw features, and they can play a great role in developing more accurate classification models. Through the extensive experiments with the Web-KB test collection, we prove that the proposed methods outperform the conventional ones.

• The Korean Journal of Mycology
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• v.24 no.2 s.77
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• pp.111-126
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• 1996

Transfer of the isolated nuclei from Lentinus edodes into protoplasts of Pleurotus florida was induced with polyethlene glycol (PEG) and $CaCl_2$. The intergeneric transfer products were classified into nuclear hybrid, heterokaryon or synkaryon, and reconstituted cell. These progenies except nuclear hybrids formed mature fruiting bodies on sawdust rice bran medium. Formation of fruit bodies was influenced by several factors such as light, temperature, nutrition and physic state of the culture media. Most of fruiting body characters were similar to those of P. florida in synkaryon and L. edodes in reconstituted cell, respectively. All these basidiocarps had clamp connections though initial heterokaryon colonies were lacking. Isozyme patterns of intergeneric progenies were quite different from those of parents. DNA polymorphisms of transfer products were also compared by random amplified polymorphic DNAs (RAPD) analysis based on polymerase chain reaction. The RAPD patterns were different from those of donor and recipient. DNA fingerprints ranged in size from 0.25 to 4.0 Kb. On the basis of RAPD, the transfer products were classified into five groups. Two synkaryon were analysed with distribution of progenies and segregation of genetic markers by random spore analyses. The genetic markers were segregated into wild type and riboflavine requiring auxotrophs.

• Korean Journal of Microbiology
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• v.41 no.4
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• pp.246-254
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• 2005

Analysis of the complete genome of Neurospora crassa reveals that at least 19 proteins contain tetratricopeptide repeat (TPR) motifs. One of them shows over $60\%$ homology to Ssn6 of Saccharomyces cerevisiae, a universal repressor that mediates repression of genes involved in various cellular processes. Mutant strains generated by RIP (repeat-induced point mutation) process showed four distinctive vegetative growth patterns and slow growth in various rates. Firstly, a mutant showed denser mycelial growth, yellow, csp, and looked like ropy mutant. Secondly, slower growth, dense mycelial, and conidial phenotype. Thirdly, extremely slower growth and aconidial. And finally, flat, tittle aerial hyphae, acon, and similar with a rco-1 RIP mutant. They are all male-fertile, yet female-sterile and produced little or no perithecium. It seems that various phenotypes were occurred depending upon mostly likely, the degree of RIP. These results indicate that this gene may be involved in several cellular possess during vegetative growth, and asexual and sexual development. Therefore it is pleiotropic. Sequence analysis of cDNA shows that it encodes a putative 102 kDa protein composed of 917 amino acids, and has six introns. It is designated rcm-1 (regulation of conidiation and morphology).

• Korean Journal of Food Science and Technology
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• v.40 no.4
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• pp.382-388
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• 2008

This study examined the nutrient components and physicochemical properties of three new potato cultivars: Gui Valley, Bora Valley, and Gogu Valley, which were acquired from PotatoValley Ltd., and compared them to the Superior cultivar that is widely distributed in food markets. Amino acid, fatty acid, and mineral compositions, as well as total starch, reducing sugar, dietary fiber, vitamin C, and phenolic acid contents were measured. The gelatinization and pasting properties of the potatoes were evaluated using differential scanning calorimetry (DSC) and a rapid visco analyzer (RVA). The three new potato cultivars showed differences for various characteristics compared to the existing Superior cultivar. The Gui valley cultivar has a high potential for processing into items such as French fries or chips, due to its high starch content and low reducing sugar content. Bora valley showed an incredibly high phenolic acid contents, and Gogu valley contained high levels of dietary fiber, minerals, vitamin C, and essential amino acids. Overall, these cultivars are expected to be highly valuable items for develpment and applications of functional food.

• Korean journal of applied entomology
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• v.38 no.2
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• pp.139-144
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• 1999

We have now constructed a novel recombinant baculovirus producing fusion protein with Autogrqha c.uliforrzica nuclear polyhedrosis virus (AcNPV) polyhedrin and green fluorescent protein (GFP). The fusion protein expressed by the recombinant baculovirus in insect cells was characterized. The GFP gene was introduced under the control of polyhedrin gene promoter of AcNPV, by fusion in the front or back of intact polyhedrin gene. The recombinant baculoviruses were named as Ac-GFPPOL or Ac-POLGFP. respectively. As expected, the 56 kDa fusion protein was expressed in the recombinant virus-infected cells. Interestingly. however, the fluorescence of GFP in the cells infected with Ac- POLGFP was only detected within the nuclei. and that was observed as polyhedra-like granular particles. In the microscopy of cells infected with Ac-GFPPOL, furthermore, GFP was detected in both cytoplasm and nuclei although most of GFP were present within the nuclei. However, fusion protein produced by recombinant virus did not form polyhedra although the fusion protein was fused with polyhedrin and GFP. It is suggested that difference of GFP location in the infected cells appear to be involved in the region of polyhedrin in the fusion protein, and the polyhedrin in the fusion protein might be responsible for the polyhedra-like granular particles present within nuclei.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.4 no.4
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• pp.338-348
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• 1999

Physical properties of mudbelt sediments in the southeastern inner shelf of Korea are studied from 14 cores. Physical properties, compressional wave velocity, and sediment texture for core sediments are analyzed. The major source of sediment in the study area is the Nakdong River. Fine-grained sediments from the river are transported northeastward by coastal circulation and the Tsushima Current, resulting in a gradual northeastward increase in porosity and a decrease in wet bulk density and velocity. The trend matches well with the bathymetry. The mean grain size appears to be the most important variable to determine the physical properties and velocity. The variations of physical properties with burial depth are dependent more strongly on sediment texture than compaction and/or consolidation. Correlations between the physical properties and the sediment texture show slight deviations from those of the continental terrace sediment in the North Pacific and inner shelf sediment in the South Sea of Korea. The velocity is higher than that of the North Pacific and the South Sea sediments between these areas. This is probably due to differences in sedimentary, environment and mineral compositions. The higher sediment velocity in the study area may also be attributed to the escape of gas from pore space which decreases void ratio.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.66-71
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• 2006

최근에 하도계획의 주된 과제는 하천환경의 보전과 창조를 위한 비용의 최소화이며, 침식과 세굴에 대한 하천관리 시설의 안정성을 확보하기 위한 질적 안전도의 최대화에 있다. 이를 위해 하도의 변화를 전제로 이동상 현상을 고려하고 저수로의 특성을 파악하여 하도계획에 반영하는 것이 필요하다. 이러한 하도계획과 관리를 위한 주요한 도구로서 하도특성조사가 제시되고 있다. 우리나라에서는 수변 생태환경의 기능회복으로의 하천환경에 관심을 기울이기 시작한 단계에 머물러 있다. 국내에서도 일본 등 선진 외국과 같이 홍수시 재해를 크게 경감하고, 자연환경을 실질적이고 체계적으로 배려하면서 경제적 하도계획 및 관리에 역점을 두어야 할 시점에 이르렀다. 이를 위해서는 우선적으로 하천이 갖고 있는 고유 특성인 하도특성에 대한 조사가 선행되어야 한다. 본 연구에서는 대상하천을 선정하고 측량, 하상재료 채취 및 분석, 유사량 채취 등의 기초조사를 수행하였으며, 이에 따른 하도특성량을 분석하였다. 대상하천은 금강유역의 지천 하류부의 약 2km 구간을 선정하였으며, 2003년${\sim}$2004년에 3회에 걸쳐 측량을 실시하고, 구간내 구룡수위표에서 유량 몇 유사량을 측정하였다. 또한 대상구간내 14개 지점에서 하상토를 채취하여 입도분석을 실시하였으며, 구룡수위표 자료를 이용하여 수문자료를 구축하였다. 수집된 기초자료를 이용하여 대상구간의 강턱유량, 특정재현기간유량, 유효유량을 산정하여 지배유량을 결정하였다. 산정된 지배유량을 이용하여 하상경사, 대표입경, 저수로내 평균수심, 에너지 경사, 마찰속도, 무차원소류력, 하폭-수심비, 수심-입경비 등의 하도특성량을 산출하여 하천환경정비를 위한 기초자료로서 활용 될 수 있도록 하였다.구에 맞는 작물 생산 및 농촌관광단지 조성을 통해 부가가치증대 및 소득증대를 꾀함으로 농촌문제 해결에 도움이 될 것으로 기대된다. 본 연구를 통해 GIS 와 RS의 기술이 농촌분야에 더 효율적으로 적용될 것으로 기대되며, 농업기술센터를 통한 정보제공을 함으로써 대농민 서비스 및 농업기관의 위상이 제고 될 것으로 기대된다.여 전자파의 공간적인 가시화를 수행할 수 있었다. 본 전자파 시뮬레이션 기법이 실무에 이용될 경우, 일반인이 전자파의 분포에 대한 전문지식을 습득할 필요 없이, 검색하고자 하는 지역과 송전선, 전철 등 각종 전자파의 발생 공간 객체를 선택하여 실생활과 관련된 전자파 정보에 예측할 수 있어, 대민 환경정보 서비스 질의 개선측면에서 획기적인 계기를 마련할 것으로 사료된다.acid$(C_{18:3})$가 대부분을 차지하였다. 야생 돌복숭아 과육 중의 지방산 조성은 포화지방산이 16.74%, 단불포화지방산 17.51% 및 다불포화지방산이 65.73%의 함유 비율을 보였는데, 이 중 다불포화지방산인 n-6계 linoleic acid$(C_{18:2})$와 n-3계 linolenic acid$(C_{18:3})$가 지질 구성 총 지방산의 대부분을 차지하는 함유 비율을 나타내었다.했다. 하강하는 약 4일간의 기상변화가 자발성 기흉 발생에 영향을 미친다고 추론할 수 있었다. 향후 본 연구에서 추론된 기상변화와 기흉 발생과의 인과관계를 확인하고 좀 더 구체화하기 위한 연구가 필요할 것이다.게 이루어질 수 있을 것으로 기대된다.는 초과수익률이 상승하지만, 이후로는 감소하므로, 반전거래전략을 활용하는 경우 주식투자기간은 24개월이하의 중단기가 적합함을 발견하였다. 이상의 행

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.10
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• pp.1430-1437
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• 2011

Synthesis conditions of cocoa butter equivalents were optimized using the response surface method (RSM) by interesterification of canola oil (Ca), palmitic ethyl ester (PEE), and stearic ethyl ester (StEE). The reaction was catalyzed by immobilized lipase (Lipozyme TLIM) from Thermomyces lanuginosa to produce structured lipids containing a composition of triacylglycerols similar to cocoa butter. Reaction conditions were optimized using D-optimal design with the three reaction factors of the substrate molar ratio of canola oil to palmitic ethyl ester and stearic ethyl ester (Ca : PEE : StEE=1:1:3, 1:1.66:5, 1:2:6, 1:2.33:7, 1:3:9, $X_1$), enzyme ratio (2~6%, $X_2$), and reaction time (30~270 min, $X_3$). The optimal conditions that minimized acyl-migration while maximizing 1-palmitoyl-2-oleoyl-3-stearoyl glycerol (POS), 1,3-distearoyl-2-oleoyl glycerol (SOS), and 1,3-dipalmitoyl-2-oleoyl glycerol (POP) were predicted, resulting in Ca : PEE : StEE=1:3:9, 6% of enzyme ratio, and 40 min of reaction time. The reaction product of structured lipids was synthesized again under the same conditions, showing 10.43 area% of acyl-migration, 25.31 area% of POS/PSO, 19.79 area% of SOS, and 11.22 area% of POP.