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http://dx.doi.org/10.5352/JLS.2014.24.2.154

The Complex Salmonella typhimurium Ghost Cells Play an Additional Role as an Immune Adjuvant  

Ha, Yeon Jo (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Kim, Seung Tae (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Kang, Ho Young (Department of Microbiology, Pusan National University)
Gal, Sang Wan (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Kim, Sam Woong (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Life Science / v.24, no.2, 2014 , pp. 154-160 More about this Journal
Abstract
Ghost cells have been recognized as eliciting humoral and cell-mediated immune responses and have also been predicted to play a role as an immune adjuvant. In this study, we used the intramuscular (IM) route to inject BALB/c mice with four vaccine groups constructed from Salmonella typhimurium ghost (STG) cells originating from different virulent strains and complex STG groups instead of heat-labile toxin (LT)-B, a type of adjuvant. Although the complex STG groups exhibited a response after a short delay, the groups showed final total IgG levels similar to those of the LT-B group, which encodes LT-B from pMMP300. The IgG1 response to the ${\chi}$3339 group was the highest response at 6 weeks, whereas IgG2a responses to the ${\chi}$3339 and JOL389 groups were higher at 6 and 8 weeks compared to those of the LT-B group. The response of vaginal sIgA to the LT-B group was generally higher than that of the other groups, whereas fecal sIgA to the LT-B group exhibited lower responses. Protection to virulent S. typhimurium in all groups was above 80%, which was similar to the LT-B group. Taken together, we suggest that STG complex groups can be used as an immune adjuvant instead of LT-B.
Keywords
Antibody; ghost; intramuscular; labile toxin (LT)-B; Salmonella;
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