• Horticultural Science & Technology
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• v.17 no.5
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• pp.578-580
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• 1999

The effects of the orchid mycorrhizal fungi isolated from Cymbidium goeringii were investigated on the growth of orchid plants. The plants, a hybrid of Cymbidium kanran Jeju${\times}$C. kanran Nangoku and Phalaenopsis were inoculated with the fungal isolates of Rhizoctonia repens (P1), R. endophytica (P2) and R. repens (P3; different from P1) on the oatmeal agars for two months. Then the orchid plants were cultivated in the greenhouse for eighteen months. The difference of plant growth after cocultivation for two months was not found among the treatments. After cultivation for four months, the growth of hybrid plants of Cymbidium kanran Jeju${\times}$C. kanran Nangoku was observed to be distinguished in the Rhizoctonia repens (P1). After cultivation in the greenhouse for eighteen months, the plant height, the number of shoots, the number of leaves, the number of roots and plant fresh weight of the hybrid of Cymbidium kanran Jeju${\times}$C. kanran Nangoku, inoculated with Rhizoctonia repens (P1), were significantly increased compared to those of the control. The plants inoculated with Rhizoctonia repens (P3) were not different and the plants inoculated with R. endophytica were poor compared to the control. The plant height and fresh weight of the Phalaenopsis, inoculated with Rhizoctonia repens (P1), increased significantly compared to those of the control. Overall, Rhizoctonia repens (P1), was the most effective for the growth of a hybrid of Cymbidium kanran Jeju${\times}$C. kanran Nangoku.

• Journal of Bio-Environment Control
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• v.24 no.3
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• pp.178-186
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• 2015

This study was carried out to determine the effects of environment controls (temperature and shading level) on germination responses and early growth of Allium thunbergii. Germination experiment was performed by pre-treatment (with low temperature and wetting treatments for 0, 20, 40 and 60 days) and temperature controls (5, 10, 15, 20, 25 and 30). And growth experiment was performed by containers (128 and 200 cavities containers) and shading level (full sunlight (control), 35%, 50% and 75% shading). Germination rate of A. thunbergii seeds were, 20 days of seed pre-treatment, the highest at $10^{\circ}C$ (81.7%) and the more temperature went up, the more germination rate went down. As a result of surveying container and shading treatments, the height, leaf area, leaf length, leaf aspect ratio (L/W) were higher under 50% shading of 128 (24.2cm, $2.76cm^2$, 22.3cm and 223.4, respectively) and 200 (22.6cm, $2.29cm^2$, 19.4cm and 190.5, respectively) cavities container. The root was grown well under full sunlight. Specially, fresh weight of shoot (leaves+stem) was higher under 50% shading of 128 (0.241g) and 200 (0.212g) cavities container. As a result of surveying the whole experiment, A. thunbergii seeds need to pre-treatment (with low temperature and wetting treatments for 20~40 days) for high germination rate. And it is judged better growth and higher yield by maintaining 50% shading of 200 cavities container.

• The Korean Journal of Pesticide Science
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• v.6 no.4
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• pp.287-292
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• 2002

To establish effective and safe control method against Phytophthora root rot caused by Phytophthora capsici on tomato in hydroponic culture, three pesticides, oxadixyl copper hydroxide 8% WP, metalaxyl copper oxychloride 15% WP, and dimethomorph. dithianon 38% WP at 4 concentration levels were tested on potato dextrose agar medium inoculated with Phytophthora capsici. All pesticides inhibited mycelial growth, but two pesticides of them, metalaxyl copper oxychloride WP and dimethomorph. dithianon WP, were selected as effective pesticides for the efficacy test in a hydroponic culture. Forty days after transplanting of tomato seedlings, 4 ml of sporangia of P. capsici (about 25 sporangi/ml) per plot was inoculated around tomato plant root, and then 5 days after inoculation, the pesticides diluted at 5,000 times were drenched 1, 2 or 3 times per plot on the culture cube at 15 days interval. Fifteen days after drenching, tomato fruits and hydroponic culture solution were sampled for the analysis of pesticide residues. Dimethomorph was detected 0.001 and 0.003 mg/kg in tomato of the plots sprayed 2 and 3 times with dimethomorph dithianon WP of which detection levels were far below compared with 1.0 mg/kg of the Korean MRL of dimethomorph on tomato. Incidences of Phytophthora root rot were $30.5{\sim}50%$ in the plots drenched at 1 or 2 times with metalaxyl.copper oxychloride WP, and $16.7{\sim}25%$ in the plots treated with dimethomorph dithianon WP. However, there was no incidence of Phytophthora root rot in the plots treated at 3 times with both of pesticides, showing no phytotoxic effect. Based on the results, the drenching of these pesticides on the culture cube could be recommended as a very safe and effective control method for Phytophthora root rot in tomato.

• The Korean Journal of Pesticide Science
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• v.7 no.4
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• pp.264-270
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• 2003

To control effectively and safely Phytophthora root rot caused by Phytophthora capsici on tomato in hydroponic culture, tank-mixing method was considered with two pesticides, metalaxyl copper oxychloride 50% WP and dimethomorph dithianon 38% WP. Forty days after transplanting of tomato seedlings, 4 mL of sporangia of P. capsici (about 25 sporangi/mL) per plot was inoculated around tomato plant roots, and at 5 days after inoculation, the pesticides tank-mixed at three dilution levels, 12,500, 25,000 and 50,000, were drenched 1, 2 or 3 times per plot on the culture cube every 15 days for metalaxyl copper oxychloride 50% WP and every 10 days for dimethomorph dithianon 38% WP. During the drenching period, the residue levels of metalaxyl and dimethomorph in hydroponic culture solution were similar to the initial levels but the level of dithianon was drastically decreased from one day after tank-mixing. In tomato drenched with metalaxyl copper oxychloride 50% WP, metalaxyl was detected $0.02\sim0.04$ mg/kg in all diluted plots. Dimethomorph was detected $0.012\sim0.021$, $0.001\sim0.006$ and $0.001\sim0.003$ mg/kg in 12,500, 25,000 and 50,000 times diluted plots, respectively, while dithianon was detected 0.005, 0.003 mg/kg in 12,500 and 50,000 times diluted plots, respectively. The detection levels of three pesticides were far below compared with the levels of Korean MRLs. Incidences of Phytophthora root rot were not found in all the plots, but phytotoxic responses were recognized in the 12,500 times diluted plots of both pesticides. Based on the above results, the drenching of the culture solution tank-mixed with these pesticides could be recommended as a very safe and effective method to control Phytophthora root rot in tomato in hydroponic culture.

• The Korean Journal of Pesticide Science
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• v.3 no.3
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• pp.60-67
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• 1999

Insecticidal activities of imidacloprid to sucking type insect pests, brown planthopper (BPH, Nilaparvata lugens) and peen peach aphid (GPA Myzus persicae), to chewing type insect pests, tobacco cut worm (TCW; Spodoptera litura) and beet armyworm (BAW, Spodoptera exigua) and to spiders, Pirata subpiraticus, Pachygnata clercki and Ummeliata insecticeps, as natural enemies were investigated by several bioassay methods. $LD_{50}$ of the chemical by topical treatment to BPH was 0.015 ${\mu}g/g$(48 hrs), while $LC_{50}s$ by leaf dipping and root zone treatment were 18.1 and 21.5 ppm, respectively. There was no difference in insecticidal activities between leaf dipping and root zone treatment. Imidacloprid also showed ovicidal effect of root zone treatment and its $LC_{50}$ was 6.8ppm $LD_{50}$ (48 hrs after treatment) of imidacloprid to GPA was $0.4{\mu}g/g$ in case of topical application and $LC_{50}s$ by leaf dipping and root zone treatment were 1.9 ppm and 13.7 ppm respectively. Leaf dipping was more effective than root zone treatment in GPA At topical application $LD_{50}$ (48 hrs after treatment) of imidacloprid to chewing type insect pests, TCW and BAW, were greater than 1,492 and $312{\mu}g/g$ and $LC_{50}s$ by leaf dipping method were 4,803 and Heater than 5,000ppm respectively. This means that imidacloprid has much less effect on chewing type insect pests, TCW and BAW. $LD_{50}$(48 hrs after treatment) of imidacloprid to wandering spiders, Pirata subpiraticus, Pachygnata clercki at topical application were greater dan $2941{\mu}g/g$ and greater than $2,976{\mu}g/g$ respectively and that to webbing spider, Ummeliata insecticeps, was 357 ${\mu}g/g$. Imidacloprid showed very low toxicity to the spiders and its selective toxicity ratios between spiders and BPH were greater than 19,600, greater than 19,800 and 23,800, respectively.

• The Korean Journal of Mycology
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• v.12 no.3
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• pp.105-110
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• 1984

Seven seed samples of corn obtained from Kangweon Provincial Office of Rural Development, Kerea were tested for seed-borne fungi, and found that all the samples tested were infected with Fusarium moniliforme to an extent of $6.0{\sim}79.5%$. Severely infected seed samples showed poor germination on blotter. Seed component plating showed that the fungus present not only in tip caps, pericarps and endosperms, but also in embryos. Heavy infection of the fungus caused severe seed rot and seedling blight in soil, but the damage was not severe and many plants grew without any symptoms when the seeds with light infection were sown in soil. However the fungus was frequently detected from inside of the stems of healthy looking seedlings. The results indicate that the fungus transmit from seed to plant systemically. In inoculation experiments, the fungus produced stem rots on corn plants of 110 days old. The cultivar of Hwangok 3 was revealed more susceptible to the fungus than that of Suweon 19.

• Korean journal of applied entomology
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• v.40 no.2
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• pp.137-141
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• 2001

This experiment was conducted to investigate the control effect of nuclear polyhedrosis virus and NeemAzal-T/S on Spodoptera litura larvae. In laboratory test, values of$ LT_{50}$ and $LT_{95}$ when treated with S. litura nuclear polyhedrosis virus (SINPV) $1$\times$10^{8}$ PIBs/ml plus NeemAzal-T/S 200 ppm were 1.94 and 8.33 days, respectively. Control effect of the combination of SINPV $1$\times$10^{8}$ plus NeemAzal-T/S 200 ppm was higher than the other concentrations. This mixed treatment could reduce LT$_{95}$ by 3 days. When SINPV alone was sprayed to the S. litura larvae reared on chinese cabbage seedling, the mortalities were 10.7~6.7% at 4 days after treatment. In combinations of SINPV plus NeemAzal-T/S at each level of concentration, the mortalities appeared faster and higher at 4 days after application than single treatment. Especially, the mortalities by combinations of SINPV $1$\times$10^{8}$ /PIBs/ml plus NeemAzal-T/S at 75~200 ppm were 100% at 9 days after treatment. The body weight of untreated larvae was increased 9.4-folds from 235 mg to 2194 mg after 7 days. However, the increasing levels of larval weight were 4.8- and 7.0-folds in the separate treatments of NeemAzal-T/S and SINPV, respectively. Whereas in the combinations of SINPV $10^{4~8}$ PIBs/ml plus NeemAzal-T/S 75~200 ppm, larval weight was increased 3.9 to 2.9-folds. These results showed that the mortality and inhibition of larval weight in the combination of SINPV and NeemAzal-T/S were highly enforced by synergistic effect.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.6
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• pp.684-696
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• 1995

A mutant for chalky endosperm and genetic male sterility (GMS) was newly developed in rice. The two characters were found to be controlled by single recessive gene which has pleiotropic effect, indicating that chalky seeds should be GMS seeds in segregating populations. Chalky seeds showed the same shape and size as normal seeds. However, starch composition of central part of endosperm was looser and shape of starch granules was rounder compared with normal endosperm, resulting in significantly lower grain weight, absolute density and grain hardness in chalky grains than in normal ones. Amylose content and gel consistency of chalky grains were much lower and harder, respectively. Male sterile plants showed much shorter plant height, poorer panicle exsertion and lesser panicle number compared with normal plants. Microspore abortion stage in pollen developmental process was observed as before meiosis. Male sterility of the mutant was stable regadless of temperature and day length. A system breeding hybrid rice using this mutant was discussed, comp ring with other systems utilizing cytoplasmic-genic male sterility(CGMS) and environment sensitive GMS(EGMS). Separation of GMS seeds in mixed seed bulks by specific gravity (1.14∼1.16g / cm3) was successful about 85∼90%. But some mixed normal plants were seemed to be easily removed by the apparent difference in growth characters at seedling stage. The highest natural outcrossing rate of this GMS line was as 17.3 % in a plot treated with 2-row pollinator, I-row GMS, and GA3 + cutting of flag leaf + pollen-scattering by rope.

• Research in Plant Disease
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• v.10 no.2
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• pp.100-104
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• 2004

Two species of Phytophthora were isolated from a leaf blight disease found on castor bean plants growing at a residential area of Hwanggeum-dong in Daegu city. One species was producing conspicuously papillate, noncaducous, ovoid to obpyriform sporangia, 31.2-58.5 ${\times}$ 25.4-44.1 ${\mu}{\textrm}{m}$ in range with an average of 46.4 $\pm$ 6.5 ${\times}$ 35.9 $\pm$ 4.7 ${\mu}{\textrm}{m}$ on simple sympodial sporangiophores, was heterothallic with oogonia globose, 22.5-35.0 ${\mu}{\textrm}{m}$ with an average of 30 $\pm$ 3.3 ${\mu}{\textrm}{m}$ in diameter, oospores plerotic, 18.8-30.0 ${\mu}{\textrm}{m}$ in range averaging 25.4 $\pm$ 2.8 ${\mu}{\textrm}{m}$, and with antheridia amphigynous. This species was identified as P. nicotianae Breda de Haan. The other species produced papillate, caducous, ovoid to ellipsoid sporangia with pedicel, 21.5-54.6 ${\times}$ 17.6-34.3 ${\mu}{\textrm}{m}$ in range with an average of 41.7 $\pm$ 7.1 ${\times}$ 28.4 $\pm$ 4.0 ${\mu}{\textrm}{m}$, and was heterothallic with oogonia globose, 21.3-26.8 ${\mu}{\textrm}{m}$ with an average of 23.0 $\pm$ 1.6 ${\mu}{\textrm}{m}$ in diameter, oospores plerotic, 17.5-23.8 ${\mu}{\textrm}{m}$ in range averaging 20.2 $\pm$ 1.8 ${\mu}{\textrm}{m}$, and with antheridia amphigynous. This species was identified as P. palmivora (Butler) Butler. Both species produced chlamydospores. Pathogenicity of the 2 speices was confirmed by foliar inoculation of castor bean seedlings. Both species have been reported to infect castor bean plants in a few foreign countries but it is the first that a disease of castor bean plants caused by either or both of the species was found in Korea.

• Journal of Bio-Environment Control
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• v.26 no.3
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• pp.201-207
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• 2017

Objective of this research was to investigate the influence of pre-plant nitrogen levels in root media on plug seedling growth of radish cv. Soksungbommu. To achieve the research purpose, a root medium, the mixture of perlite, coir dust, and peatmoss (volume percentage of 30:35:35) was formulated and the N levels incorporated during mixing were controlled to 0, 100, 250, 500, 750, 1,000, and $1,500mg{\cdot}L^{-1}$. Then, the seeds were sown into 72-cell plug trays in which the root medium was packed. The measurements of growth and analysis of tissue and root media were conducted 2 and 4 weeks after sowing. Elevation of pre-plant N levels raised EC and turned down pH of root media. But, as seedling grew, the pH rose and EC get lowered in all treatments. The EC as well as $NH_4-N$ and $NO_3-N$ concentrations of root media declined gradually until week 2, but those declined sharply between weeks 2 to 4. The seedling growth 2 weeks after sowing showed quadratic response to pre-plant N levels with the highest growth in $250mg{\cdot}L^{-1}$ treatment and lagging growth in the treatments of lower or higher N levels than $250mg{\cdot}L^{-1}$. The seedling growth 4 weeks after sowing showed also quadratic response with the highest growth in $500mg{\cdot}L^{-1}$ treatment. The tissue N contents were get higher and those of K, Ca, and Mg were get lower as pre-plant N levels were elevated. Above results suggest that lower than $250mg{\cdot}L^{-1}$ in pre-plant N levels is optimistic for growth of plug seedling and avoidance of toxic injury in very young stage.