• Tuberculosis and Respiratory Diseases
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• v.44 no.4
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• pp.871-888
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• 1997

Background : It is now thought that the earliest manifestation of idiopathic pulmonary fibrosis is alveolitis, that is, an accumulation of inflammatory and immune effector cells within alveolar walls and spaces. Inflammatory cells including alveolar macrophages and resident normal pulmonary tissue cells participate through the release of many variable mediators such as inflammatory growth factors and cytokines, which contribute to tissue damage and finally cause chronic pulmonary inflammation and fibrosis. This study was performed to investigate the source and distribution pattern of transforming growth factor-${\beta}_1$(TGF-${\beta}_1$), platelet derived growth factor(PDGF), basic fibroblast growth factor(bFGF), interleukin 1(IL-1), interleukin 6(IL-6), tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and the role of these mediators on bleomycin(BLM)-induced pulmonary injury and fibrosis in rats. Method : Wistar rats were divided into three groups(control group, BLM treated group, BLM and vitamine E treated group). Animals were sacrificed periodically at 1, 2, 3, 4, 5, 7, 14, 21, 28 days after saline or BLM administration. The effects were compared to the results of bronchoalveolar lavage fluid analysis, light microscopic findings, immunohistochemical stains for six different mediators(TGF-${\beta}_1$, PDGF, bFGF, IL-1, IL-6 and TNF-$\alpha$) and mRNA in situ hybridization for TGF-${\beta}_1$. Results : IL-1 and IL-6 are maximally expressed at postbleomycin 1~7th day which are mainly produced by neutrophils and bronchiolar epithelium. It is thought that they induce recruitment of inflammatory cells at the injury site. The expression of IL-1 and IL-6 at the bronchiolar epithelium within 7th day is an indirect evidence of contribution of bronchiolar epithelial cells to promote and maintain the inflammatory and immune responses adjacent to the airways. TNF-$\alpha$ is mainly produced by neutrophils and bronchiolar epithelial cells during 1~5th day, alveolar macrophages during 7~28th day. At the earlier period, TNF-$\alpha$ causes recruitment of inflammatory cells at the injury site and later stimulates pulmonary fibrosis. The main secreting cells of TGF-${\beta}_1$ are alveolar macrophages and bronchiolar epithelium and the target is pulmonary fibroblasts and extracellular matrix. TGF-${\beta}_1$ and PDGF stimulate proliferation of pulmonary fibroblasts and TGF-${\beta}_1$ and bFGF incite the fibroblasts to produce extracellular matrix. The vitamine E and BLM treated group shows few positive cells(p<0.05). Conclusion : After endothelial and epithelial injury, the neutrophils and bronchiolar epithelium secrete IL-1, IL-6, TNF-$\alpha$ which induce infiltration of many neutrophils. It is thought that variable enzymes and $O_2$ radicals released by these neutrophils cause destruction of normal lung architecture and progression of pulmonary fibrosis. At the 7~28th day, TGF-${\beta}_1$, PDGF, bFGF, TNF-$\alpha$ secreted by alveolar macrophages sting pulmonary fibroblasts into proliferating with increased production of extracellular matrix and finally, they make progression of pulmonary fibrosis. TNF-$\alpha$ compares quite important with TGF-${\beta}_1$ to cause pulmonary fibrosis. Vitamine E seems to decrease the extent of BLM induced pulmonary fibrosis.

• Microbiology and Biotechnology Letters
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• v.17 no.4
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• pp.349-357
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• 1989

Uncooked starch can be effectively saccharified in an enzyme reaction system containing attrition-milling media. To develope the high efficiency bioattritor, an agitated bead type bioreactor was constructed, and its effectiveness was evaluated. The optimal operation condition of bioattritor was found to be 300 g glass bead/L, 200 rpm, standard type impeller for 220 g/L of uncooked corn starch. The torque under the various operational conditions were also measured. The interrelation-ship between energy consumption for agitation of attrition-milling media and enhanced extent of saccharification of uncooked starch was evaluated, Power consumption was measured to be around 1.53 watt/L under the optimal operation condition. The attrition coupled enzyme reaction system is identified to tie a very excellent energy saying process for saccharification of uncooked starch, and seems to have a bright prospect of industrial application.

• Korean Journal of Food Science and Technology
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• v.29 no.5
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• pp.847-853
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• 1997

Attempts were made to determine the compositions of Opuntia ficus-indica var. saboten for the utilization as food materials. The major components of Opuntia ficus-indica and aloe in proximate compositions were nitrogen free extract. Total mineral contents of stem, fruit and seed of Opuntia ficus-indica were 9400.8, 6151.2 and 1096.8 mg%, respectively, and their major minerals were Ca, P and Mg. The major free amino acids of fruit were tyrosine, proline and arginine, those of stem were glycine and arginine and that of seed was glutamic acid. The major amino acid of fruit was glutamic acid, those of stem were glycine and arginine and those of seed were glutmic acid and arginine. Vitamin C contents of fruit and stem were 163.8 and 71.2 mg% but not presented in seed. Vitamin A was also not presented in fruit, stem and seed. Contents of total polyphenols and flavonoids were changed by extraction solvent and temperature. Total polyphenols of fruit were higher than those of stem and seed. Total flavonoids of fruit were similar to those of stem.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1021-1025
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• 2001

Physicochemical properties of Rubus coreanus Miquel were examined. Unripened fruit, ripened fruit and leaf of Rubus coreanus Miquel were used as samples. The contents of water, crude protein, crude fat, crude fiber, dietary fiber, and crude ash were studied. The pH, soluble solid content, acidity and color were also measured. In terms of free sugar composition, concentration of glucose and fructose found in unripened fruit had increased in ripened fruit and sucrose was contained in ripened fruit. The major free sugars of leaf were glucose, fructose and sucrose. Citric acid, succinic acid and fumaric acid were found in all three sample groups, unripened fruit, ripened fruit and leaf. The citric acid showed the highest concentration in the unripened fruit as the concentration of 1.21%. Fifteen types of amino acids were analysed from the unripened and ripened fruit of Rubus coreanus Miquel. The leaf contained methionine in addition to those fifteen type amino acids in fruits. The glutamic acid contents of unripened fruit, ripened fruit and leaf were 411, 246.59 and 898.42 mg%, respectively. Among the minerals studied in this research Rubus coreanus Miquel held high level of potassium. The potassium concentrations of unripened fruit, ripened fruit and leaf were 645.07, 216.53 and 815.78 mg%, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.5
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• pp.476-481
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• 1994

The lipase activity of crude enzyme extracted from the molded sardine meal(MSM) 'koji', and lipid classes and fatty acid composition of neutral lipid(NL) and phospholipid (PL) of MSM 'koji' were investigated. The optimum pH and temperature of the crude enzyme was 8.0 and $40^{\circ}C$ using olive oil emulsion as a substrate, but the residual activity depended on pH and temperature. Total lipid contents of the MSM 'koji' consisted of 15.33g/100g NL, 5.45g/100g PL respectively. The major lipid classes of NL were triglyceride ($53.4\%$) and free fatty acid($28.1\%$), that of PL were phosphatidylethanol($32.7\%$), phosphatidylinositol($25.5\%$), phosphatidylcholine($25.1\%$), lyso-phosphatidylcholine($7.3\%$) and sphingomyeline($3.5\%$). The prominent fatty acid fractions of NL were 16:0, 22:6, 18:1 n-9, 18:2n-6 and 20:5, with PL fractions the major fatty acid ratios were 22:6, 16:0, 18:1n-9, 18:2n-6 and 20:5, in the same respective order.

• Tuberculosis and Respiratory Diseases
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• v.61 no.5
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• pp.479-483
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• 2006

Acute fibrinous and organizing pneumonia (AFOP) is a histological pattern consisting of prominent intra-alveolar fibrin and organizing pneumonia, with out hyaline membranes or prominent eosinophilia. The clinical manifestations of AFOP resemble those of acute lung injury such as acute interstitial pneumonia (AIP). However, the classic histological patterns of AFOP differ from diffuse alveolar damage (DAD), bronchiolitis obliterans with organizing pneumonia (BOOP) or acute eosinophilic pneumonia (AEP). The characteristic intra-alveolar fibrin ball and lack of classic hyaline membrane are the predominant histological features of AFOP. Although some reports suggest that its clinical course is less catastrophic than DAD, the clinical entity that distinguishes AFOP from DAD has not been established. We present a case of pathologically demonstrated AFOP in a 79-year-old man. The radiological findings of our case were similar to those of DAD, presented with diffuse bilateral lung infiltrations. However, despite the rapid development of respiratory failure, the patient had a better response and outcome to steroid therapy than what would be expected for DAD.

• Journal of Periodontal and Implant Science
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• v.23 no.1
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• pp.97-108
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• 1993

One of the important initial events required for periodontal regeneration is the attachment and subsequent spreading of periodontal ligament cells on the root surface. The purposes of this study is to investigate the attachment and spreading pattern of human periodontal ligament cell on the surface of glass slides. After establishment of a cell line of the primary cell culture from the periodontal ligament of 1st premolar teeth which were extracted for the purpose of orthodontic treatment, author dispersed the cells at $5{\times}10^3\;cells/ml$ into the each 35mm culture petri-dish containing 2 glass slides. To observe the morphological changes of the cells which attached to the surfaces of glasses at every designed time schedule, author used the inverted phase contrast microscope and scanning electron microscope. During the whole experiment culture condition was at $37^{\circ}C$, 100% Humidity, 5% $CO_2$ gas incubator. The following results were obtained. Periodontal ligament cells showed spherical outline and started to attach to glass surface by basal sytoplasmic extension after 10min in culture. After 30min in culture, periodontal ligament cells were attached to glass surface by well - developed filopodia which protruded from the lamellipodia. The cell surface is covered with bubble-like structures and occasional microvillus can be seen with diffculty among these structures. After 1.5hr in culture, peridontal ligament cells shhowed radially well-spread cytoplasm and the nucleus was centered on its cytoplasm. Unspread central region of the cell was covered with numerous microvilli. The change of cell attachment and spreading pattern was manifest at 6hr in culture. At this time, periodontal ligament cell showed elongated outline and an oval-shaped nucleus. After 12hr in culture, periodontal ligament cells showed more stretched fibroblast-like appearance with polarity. Two long lamellipodia can be seen around the both terminal ends of cells. After 24hr in culture, periodontal ligament cells showed spindle shapes and an oval-shaped nucleus was slanted toward one side of the cell.

• Journal of the Mineralogical Society of Korea
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• v.29 no.3
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• pp.123-129
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• 2016

This study is conducted to estimate the firing temperature and condition of the 4-6th Century earthenwares excavated from the ancient tombs in the Seonggok-ri, Cheongdo through mineralogical approaches, and to contrast to the characteristics of the earthenware from the Changnyeong the adjacent area to the Cheongdo. The Cheongdo earthenware was mainly composed of quartz, pores and vitric matrix with minor parts of feldspars, felsic volcanic fragments and opaque minerals, of which quartz crystals frequently show embayed texture and pores was partly filled with amorphous quartz. Mullite, hematite and cristobalite were found by XRD spectrometer additionally, and in the SEM image the Mullite contents are evenly distributed from the central part to the marginal part. As a result, the firing temperature of the Cheongdo earthenware could be 1,100 to $1,200^{\circ}C$, which is higher than that of the Changnyeong. Distribution characteristics of mullite in the Cheongdo and the Changnyeong earthenware indicate that the Cheongdo earthenware was under the longer firing time or more homogeneous thermal condition in the kiln than the Changnyeong.

• The Journal of the Petrological Society of Korea
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• v.16 no.2 s.48
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• pp.59-72
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• 2007

Kilauea volcano located in the southern part of Hawaii island chains has been formed by continuous and sporadic eruption activities from 16 century to September of 1982. Kilauea volcano was investigated from collecting the samples of basalt to identify the geochemical characteristics of the lavas. Olivine, clinopyroxene, plagioclase, and opaque minerals with glass groundmass are observed. Clinopyroxene formed glomerophopyritic texture with plagioclase and zoning texture was observed in some of plagioclase phenocrysts. Major elements such as $K_2O$, $P_2O_5$, $TiO_2$ increased with respect to MgO. $Al_2O_3$ and CaO show the kink on Marker diagram due to the crystal fractionation of plagioclase and clinopyroxene with plagioclase respectively. Olivine has a wide range of Fo% ranging from 60 to 90, and MnO and FeO in the olivine are decreased with increasing Fo% oppositely increased $SiO_2$, MgO, and NiO. Ni in the whole rock is controlled not only by the mode of olivine but also by the Ni abondance in the olivine.

• Korean Journal of Heritage: History & Science
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• v.45 no.1
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• pp.70-85
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• 2012

Uiseong Tamni-ri Five-storied Stone Pagoda is composed of andesitic tuff and partially combined with tuff breccia and fine-grained granite. The andesitic tuff is identical to basement rock of Geumseongsan Mountain based on lithological, mineralogical and geochemical characteristics. The pagoda has suffered physical weathering such as crack and scaling, discoloration and biological colonization with complex reaction. Expecially, dark gray and brown discoloration appeared whole over the surface of the pagoda, and three to five-layered exfoliation and granular disintegration dominantly occurred in the fourth and fifth roof stones. It is assuming that the stone elements of the pagoda are evaluated as third to forth grades (average third grade) of weathering compared to fresh rock in Geumseongsan Mountain. The physical strength of the stone elements shows low values in the south and west sides of the pagoda that corresponds high weathering degree of the west side due to exfoliation, crack and granular disintegration. It is necessary to investigate the pagoda for precise deterioration assessment, monitoring and conservation treatment.