• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.6 no.3
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• pp.33-44
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• 2007

Prohibition of lane changes in bridges and tunnels have been many problems in throughputs of expressways caused by heavy vehicles and slow-moving traffics. Nevertheless, those are constructed actively by the general trends, which are preservation of environment and ecosystem are more important, because mountainous districts are about 70% across the whole extent of Korea. In this paper, the proper design standards for permission of lane changes in bridges and tunnels classified into structure, safe, and driver's conveniences are suggested as follows. 1. Right shoulder should have more than 2.5m in bridges and tunnels. 2. Sufficient equipments of guidance like as directional signs, fingerposts, variable message signs, and markings should be established to smooth and safe lane changes of drivers. 3. Snow melting systems should be established in bridges worried about freezing. 4. Tunnels must be not only satisfied standards for prevention of disasters (2004.11) and lighting rules (KSA 3703), but also established anti-freezing facilities in entrance and exit. 5. The drivers should have honed on their car lights.

• Biomedical Science Letters
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• v.2 no.2
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• pp.257-265
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• 1996

Simple stain and electron microscopic in situ hybridization is studied and applied for the identification of rabbit haemorrhagic disease viral RNA in a unicrylated preparation of the liver after innoculation of rabbit haemorrhagic disease virus. Hybridization for detection of viral RNA in unicryl embedded tissues using complementary 84 bases oligonucleotide probe labelled by biotin CE-phosphoramidite compared with 4717∼4800 sequences of rabbit haemorrhagic disease virus, modified hybridization protocol and antibiotin antibody-l0nm gold as signal marker. The best results were obtained in 0.02% glutaraldehyde, Unicryl resin cell block, biotinylated oligonucleotide probes, antibiotin-l0nm gold. In this report, RHD viral RNA was distributed widely within the mitochondria and nucleus of liver cell by electron microscopic in situ hybridization. In situ hybridization has become a standard method for localizing DNA or RNA sequences in tissue or celt preparation. In situ hybridization is detected the virus genome in the cells and tissue as specifically compared with others nucleic acid hybridization method.

• Journal of Digital Convergence
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• v.16 no.7
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• pp.249-257
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• 2018

The purpose of this study is to construct a virtual reality simulation environment for searching and evaluating evaluation criteria for road safety facilities with new technologies. Virtual reality simulation requires high realism and accurate behavior data extraction. To do this, we used the Unreal Engine to create the environment by dividing it into an external environment and a vehicle environment. After that, a sample simulation for the luminescent road markers and preliminary experiments were conducted. As a result, luminescent road markers showed better 5m interval than 10m interval. It can be confirmed that it can be used in the simulation for searching the evaluation criteria for the new road safety facilities that incorporate the new technology in the future. In the future, it will be possible to simulate various environments by adding modeling and sample components for other road facilities.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2005.11a
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• pp.167-170
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• 2005

충주댐 상${\cdot}$하류 하천 주요 지점의 수위-유량 상관관계를 분석하기 위하여 2004년 3월부터 12월까지 266일간 4개 수위국 지점을 대상으로 현지조사 및 하천측량을 실시하고, 홍수기 및 평${\cdot}$갈수기에 유량측정을 실시하여 수위-유량 관계식을 유도하였으며, 이를 바탕으로 유출량산정과 유황분석을 실시하였다. 평${\cdot}$갈수기 신뢰성 있는 수위-유량 관계곡선을 유도하기 위하여 실시간으로 관측되는 수위를 확인(www.wamis.go.kr)하여 측정지점의 수위를 균형있게 분포되도록 하여 신뢰도 향상을 기할 수 있었다. 2004년 홍수기, 평갈수기에 측정한 수위-유량성과를 이용하여 수위-유량관계식을 홍수기, 평갈수기로 나누어 유도하였으며, 표준편차와 상관계수를 검토한 결과 대체로 이차식 형과 지수식 형에 의한 계산치가 실측치에 근접하는 것으로 나타났다. 본 연구에서 작성한 수위-유랑관계식의 신뢰성 검증을 하기 위하여 2004년 7월 11일에서 7월 20일까지의 홍수시의 시간별 유출량자료와 2003년 11월 1일에서 2004년 10월 31일까지의 일별 유출량자료를 분석한 결과, 각 수위표지점의 첨두유량과 유출율 관계를 댐유입량 자료과 비교 검토하여 또한 HEC-RAS를 이용하여 수면형을 계산하여 수위-유량 관계식의 신뢰도가 높은 것을 확인할 수 있었다.

• Development and Reproduction
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• v.6 no.1
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• pp.55-66
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• 2002

Embryonic stem cells(ES cells) are derived from the inner cell mass(ICM) of blastocysts, which have the potentials to remain undifferentiated, to proliferate indefinitely in vitro, to differentiate into the derivates of three embryonic germ layers. ES cells are an attractive model system for studying the initial developmental decisions and their molecular mechanisms during embryogenesis. Additionally, ES cells of significant interest to those characterizing the various gene functions utilizing transgenic and gene targeting techniques. We investigated the effects of reproductive hormones, gonadotropins(GTH) and steroids on the induction of differentiation and expressions of their receptor genes using the newly established mouse ES cells. We collected the matured blastocysts of inbred mice C57BL/6J after superovulation and co-cultured with mitotically inactivated STO feeder cells. After 5 passages, we confirmed the expression alkaline phosphatase(Alk P) activity and SSEA-1, 3, 4 expressions. The protocol devised for inducing ES differentiation consisted of an aggregation steps, after 5 days as EBs in hormone treatments(FSH, LH, E$_2$, P$_4$, T) that allows complex signaling to occur between the cells and a dissociation step, induced differentiation through attachment culture during 7 days in hormone treatments. Hormone receptors were not increased in dose-dependent manner. All hormone receptors in ES cells treated reproductive hormones were expressed lower than those of undifferentiated ES cell except for LHR expression in E$_2$-treated ES cells group. After hormone induced differentiation, at least some of the cells are not terminally differentiated, as is evident from the expression of Oct-4, a marker of undifferentiated. To assess their differentiation by gene expression, we analyzed the expression of 7 tissue-specific markers from all three germ layers. Most of hormone-treated group increased in the expression of gata-4 and $\alpha$ -fetoprotein, suggesting reproductive hormone allowed or induced differentiation of endoderm.

• Journal of Life Science
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• v.21 no.4
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• pp.534-541
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• 2011

Retinoic acid (RA), a metabolite of vitamin A, is known to regulate dedifferentiation of rabbit articular chondrocytes. The regulatory mechanism of dedifferentiation by RA is not yet understood. Thus, the effect of RA on the regulation of nitric oxide (NO)-induced dedifferentiation was investigated in rabbit articular chondrocytes. RA caused loss of the differentiated chondrocyte phenotype as demonstrated by inhibition of type II collagen expression and proteoglycan synthesis. RA also accelerated NO-induced dedifferentiation in rabbit articular chondrocytes as detected by expression of type II collagen and Sox-9 using Western blot analysis and production of sulfated proteoglycan using Alcain blue staining. Further, RA potentiated NO-induced activation of ERK. Inhibition of ERK with PD98059 (PD) recovered the expression of type II collagen and Sox-9 and production of sulfate proteoglycan in NO-induced dedifferentiated chondrocytes by RA treatment. Our findings suggest that RA accelerates NO-induced dedifferentiation of rabbit articular chondrocytes via the ERK pathway.

• Parasites, Hosts and Diseases
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• v.28 no.2
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• pp.71-78
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• 1990

To assess the role of cAMP on the growth and proliferation of Toxoplasma in HL-60 cells we tested the effect of exogenous cAMP and cAMP analogues to the co-culture system of Toxoplasma and HL-60 cells. cAMP, dbcAMP, and br-cAMP stimulated the growth of Texoplasma at a specific concentration, i.e., 100 mM, l00 mM, and 10-1 mM, respectively. There were differences in growth induction kinetics and in the rate of promotion. These results were further verified by treating the co-culture with adenylate cyclase activator, pNHppG, cAMP phosphodiesterase activators, imidasole and A23187, and cAMP phosphodiesterase inhibitors, IBMX, compound 48/80, and theophylline, separately. When the cytosolic cAMP levels increased by the reagents mentioned above, Toxoplasma in the cytoplasm of HL-60 cells stimulated to proliferate more rapidly with concentration-dependent modes compared to the control, and vice versa. It is suggested that some mechanisms are activated by the high levels of cAMP in the cytoplasm, which result in the stimulation of Toxoplasma proliferation.

• Parasites, Hosts and Diseases
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• v.30 no.4
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• pp.309-322
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• 1992

In order to observe the antigenic localization in the tissues of Metngonimus yokogawai in growth stages, immunogoldlabeling method was applied to using serum of the cat which Infected with isolated metacercariae from Plecoglossus aztivelis. The sectioned worm tissues from each growth stages were embedded in Lowicryl HM 20 medium, stained with infected serum IgG and protein A gold complect (particle size: 12 nm) and observed by electron microscopy. In the worm tissues of all experimental groups, the geld particles were specifically concentrated on the tegumental synch- tium and cytoplasm of the tegumental cell as well as the secretory granules in the parenchymal tissue. In the 16th and 20th week grown worm tissues, the gold particles were specifically concentrated on the vesicles in the tegumental syncytium and cl·toplasm of the tegumental cell. The gold particles were specifically concentrated on the caecal epithelia of the 4th, 8th and 12th week growth groups but slightly concentrated on those of the 16th and 20th week.

• Korean Chemical Engineering Research
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• v.60 no.3
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• pp.398-406
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• 2022

The outer membrane of Gram-negative bacteria is the outermost layer of cellular environment in which numerous biophysical and biochemical processes are in action sustaining viability. Advances in cell engineering enable modification of bacterial genetic information that subsequently alters membrane physiology to adapt bacteria to specific purposes. Surface display of a functional molecule on the outer membranes is one of strategies that directs host cells to respond to a specific extracellular matter or stimulus. While intracellular expression of a functional peptide or protein fused to a membrane-anchoring motif is commonly practiced for surface display, the method is not readily applicable to exogenous or large proteins inexpressible in bacteria. Chemical conjugation at reactive groups naturally occurring on the membrane might be an alternative, but often compromises fitness due to non-specific modification of essential components. Herein, we demonstrated two distinct approaches that enable site-specific decoration of the outer membrane with a fluorescent agent in Escherichia coli. An unnatural amino acid genetically incorporated in a surface-exposed peptide could act as a chemoselective handle for bioorthogonal dye labeling. A surface-displayed α-helical domain originating from a part of a selected heterodimeric coiled-coil complex could recruit and anchor a green fluorescent protein tagged with a complementary α-helical domain to the membrane surface in a site- and hetero-specific manner. These methods hold a promise as on-demand tools to confer new functionalities on the bacterial membranes.

• Journal of Aquaculture
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• v.19 no.3
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• pp.166-172
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• 2006

Induced androgenesis, a form of artificial parthenogenesis is an important tool for the generation and use of genetically isogenic or clonal fish strain. An optimized protocol for the genetic inactivation of mud loach (Misgurnus mizolepis) oocytes (i.e. production of androgenetic haploid) was developed using UV-irradiation. Various dose levels of UV significantly affected the fertilizing capacity of the eggs, hatchability of embryos and incidence of haploidy. Based on the extensive examinations of treatment conditions on embryo viability and haploid incidence, the optimum dose level of UV irradiation was turned out to be $10,800ergs/mm^2$ with 56.9% of hatching success and 94.6% of haploidy. The overall yield of putative androgen under optimized treatment condition was more than 50% out of total eggs inseminated. The success of androgenetic reproduction of haploid genome was verified by flow cytometry and PCR amplification of transgene that is exclusive to either one of parental sexes. However, a small portion $(8\sim11%)$ of presumed androgenetic haploid larvae was proven to contain residual DNA fragment(s) from maternal parent.