• Journal of Korean Society of Forest Science
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• v.77 no.2
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• pp.199-207
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• 1988

Three clones of Populus alba ${\times}$ P. grandidentata have been tested for susceptibility to Agrobacterium tumefaciens strains A281 and A348. We determined the optimum concentration of kanamycin sulfate for effective selection of leaf disc-derived, transgenic tissues transformed using Agrobacterium binary vector pGA472 containing a neomycin phosphotransferase gene (NPT-II) which confers kanamycin resistance. Of the wild type Ti plasmids contained by the two Agrobacterium strains, pTiBo542 of strain A281 appears to be best suited to serve as a helper plasmid for binary vector systems. A relatively low concentration (10mg/l) of kanamycin sulfate inhibited adventitious shoot initiation from leaf discs on regeneration medium. Transformed kanamycin-resistant calli were obtained by culturing Agrobacterium inoculated leaf discs on selective regeneration medium. The transformed kanamycin-resistant calli continued to grow on regeneration media supplemented with kanamycin sulfate to levels of 50 and 200mg/l. The growth of non-co-cultivated control calli was severely inhibited on regeneration medium containing 50mg/l kanamycin sulfate.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.281-287
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• 1999

Codonopsis lanceolata ("Deoduck" in Korea) is a perennial herb, and belongs to family, Campanulaceae. Its taproot is used a good source of a wild vegetable as well as an herbaceous medicine. In this study, to develop a bialaphos-resistant transgenic Codonopsis, seed germination mechanism and somatic embryogenesis of the plant were investigated, and Agrobacterium-mediated transformation with bar gene encoding phosphinothricin acetyltransferase (PAT) was performed. Attempt were made to regenerate plant from cells via somatic embryogenesis. When the cotyledons, nodes and leaf disks were cultured on MS medium containing 2,4-D and zeatin, embryogenic calli were induced. Upon transferring the somatic embryos to N6 solid medium without plant growth regulators, they developed into plantlets under continuous illumination. All plants were dead on MS basal medium containing 10 mg/L phosphinothricin (PPT) and Basta, respectively. The explants did not produce calli in the medium containing 200 mg/L kanamycin. The explants were cocultured with Agrobacterium tumefaciens for 2 days, and transformants were selected in MS basal medium containing 1.0 mg/L 2,4-D, 100 mg/L kanamycin and 500 mg/L carbenicillin. After the selection, embryogenic calli were induced and then somatic embryos were produced by subsequent subculturing. The somatic embryos were germiated on N6 basal medium containing 200 mg/L kanamycin and 500 mg/L carbenicillin. PCR analysis showed that nptII and bar genes were introduced in the Deoduck transformants. After the confirmation of bar gene expression in RNA and protein level, the transgenic Deoduck will be used to study the genetics of filial generation with the herbicide control gene, bar.gene, bar.

• Journal of Life Science
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• v.33 no.11
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• pp.859-867
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• 2023

Lupeol is a type of pentacyclic triterpene that has been reported to have therapeutic effects for treating many diseases; however, its effect on insulin resistance is unclear clear. This study examined the inhibitory effect of lupeol on the serine phosphorylation of insulin receptor substrate-1 in insulin resistance-induced 3T3-L1 adipocytes. 3T3-L1 cells were cultured and treated with tumor necrosis factor-α (TNF-α) for 24 hours to induce insulin resistance. Cells treated with different concentrations of lupeol (15 μM or 30 μM) or 100 nM of rosiglitazone were incubated. Then, lysed cells underwent western blotting. Lupeol exhibited a positive effect on the negative regulator of insulin signaling and inflammation-activated protein kinase caused by TNF-α in adipocytes. Lupeol inhibited the activation of protein tyrosine phosphatase-1B (PTP-1B)-a negative regulator of insulin signaling-and c-Jun N-terminal kinase (JNK); it was also an inhibitor of nuclear factor kappa-B kinase (IKK) and inflammation-activated protein kinases. In addition, Lupeol downregulated serine phosphorylation and upregulated tyrosine phosphorylation in insulin receptor substrate-1. Then, the downregulated phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway was activated, the translocation of glucose transporter type 4 was stimulated to the cell membrane, and intracellular glucose uptake increased in the insulin resistance-induced 3T3-L1 adipocytes. Lupeol may improve TNF-α-induced insulin resistance by downregulating the serine phosphorylation of insulin receptor substrate 1 by inhibiting negative regulators of insulin signaling and inflammation-activated protein kinases in 3T3-L1 adipocytes.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.12 no.3
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• pp.99-103
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• 1998

We propose a nonlinear feedback controller that can control the induction motors which have been applied to many electrical facilities with high dynamic performance by means of decoupling of moor speed and rotor flux. The nonlinear feedback controller needs the information on some motor parameters. Among them, rotor resistance varies greatly with machine temperature. Anew recursive adaptation algorithm for rotor resistance which can e applied to our nonlinear feedback controller is also presented in this paper. To demonstrate the practical significance of our results, we present some experimental results.

• Proceedings of the KIPE Conference
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• 2015.07a
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• pp.83-84
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• 2015

본 논문에서는 무효전력 오차를 야기하는 유도성-저항성 복합성의 불평형 임피던스 선로 하에서 가상 인덕터와 가상 저항을 사용한 드룹 제어 기반의 병렬형 인버터 시스템에 관해 다루고 있다. 여기서 무효 전력 분담 에러는 각 선로 임피던스의 전압 강하를 고려함에 따라 개선 가능하다. 그러나 병렬형 인버터 시스템이 고전류, 고용량일 경우 지령 출력 전압 크기는 정격 전압 크기에 미치지 못하게 되며 이는 가상 인덕터 및 저항값과 출력 전류의 곱을 통한 가상 임피던스의 전압강하 산출법에 기인한다. 이러한 이유로 본 논문에서는 기존의 드룹 방식에 선로 임피던스 전압 강하 뿐만 아니라 가상 임피던스의 전압 강하도 추가되었고 이를 통해 병렬형 인버터의 전압 출력이 정격 출력값의 범위 안에서 구동하도록 하였다. 가상 인덕터와 가상 저항의 적용법에 대한 비교에 기반을 둔 제안된 드룹 방식은 PSIM 시뮬레이션을 통해 검증되었다.

• The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.10 no.5
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• pp.57-65
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• 1996

초고속 정전유도형 정전전동기의 개발의 위한 기초연구로서 회전자 물질의 표면저항률, 비유전율 및 전하완하시정수의 변화에 따른 회전자의 회전속도특성을 조사하였다. 회전자표층물질의 비유전율 및 표면저항률은 클수록 회전자의 회전속도는 증가하였으며, 또한 이 두 요소를 곱한 회전지표면에 유도된 전하의 완화시정수가 클수록 회전속도는 증가하였다. 한편 회전자표층물질로서 도전성물질(Ti)을 폴리프로필렌 위에 불연속적으로, 즉 띠의 모양으로 증착한 시료를 사용하여 띠의 폭 및 경사각의 변화에 따른 회전자의 회전속도특성 및 토크 특성을 조사하였다. 이 경우 띠의 폭이 적어질수록 회전자의 회전수는 지수함수적으로 증가하였으며, 등간격으로 세분화한 것을 회전자의 축에 대해서 회전방향으로 경사각$\theta$만큼 기울였을 때 $\theta$=60。 및 150。일 때 회전자의 회전속도가 가장 큰 것으로 나타났으며, 특히 경사각 $\theta$=0。일때에 비해서 약 125[%]정도 높은 회전속도를 보였다. 최대토크 및 최대출력은 각각 {{{{25$\times$ { 10}^{ -6} [Nm]}}}}및 11.5[mW]이었다.

• Proceedings of the KIEE Conference
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• 1997.07a
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• pp.115-117
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• 1997

본 논문에서는 유도전동기의 적응 비선형 제어에 대해 논한다. 제어 목적은 회전자저항의 불확실성에도 불구하고 유도전동기의 속도 및 자속을 분리하여 제어하는 것이며, 이를 위해 백스테핑(Backstepping) 기법을 사용한 비선형제어기와 회전자 저항 추정기를 결합한다. 제안된 적응제어시스템은 내부의 모든 변수가 유계(Bounded)되어 있다는 점에서 안정하며, 더불어 그 개선된 성능을 모의실험을 통해 보인다.

• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.596-602
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• 2019

In September 2017, the rhizospheric soil of Tetragonia tetragonoides (Pall.) Kuntze was further sampled. One hundred and thirty eight species of microorganisms were isolated from the soil. Indole-3-acetic acid (IAA) production, siderophore production, and phosphate degradation were examined in order to confirm bacterial growth from isolated microorganisms. As a result, most strains were able to produce auxins or siderophores and to solubilize phosphate. In addition, 138 isolated strains were treated with tobacco extract and conferred pathogen resistance to host plants upon treatment. As a result, 35 strains that were able to reduce pathophysiology by more the 60% were selected. Among them, 6 strains with high induced systemic resistance (ISR) activity were found. All of these strains belong to the genus Bacillus according to the 16S rDNA sequence analysis. Bacillus aryabhattai KUDC6619 showed outstanding effects with reduced infection in tobacco and pepper plants. Probably, these Bacillus species play a beneficial role by association with T. tetragonoides for its survival in the harsh conditions found on the island of Dokdo.

• Journal of the Korean Vacuum Society
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• v.3 no.4
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• pp.457-465
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• 1994

유도결합 플라즈마의 해석식으로 양극성 확산, 정상상태를 가정한 확산식, 열평형식과 변위전류 를 무시한 맥스웰 식을 사용하였다. 해석기법으로는 유한 차분법과 적분법을 축대칭 2차원(R, Z)모델에 적용하였다. 유도 결합 플라즈마장치의 RF 전력, 압력, 석영창 두께, 차폐부 높이에 따른 전자온도, 전자 밀도, 등가 정항 등가 인덕턴스 효율 결합계수 K, Q-factor의 변화를 구하였다. 특히 등가정항은 진공챔 버, 차폐부 및 코일의 손실저항을 고려하여 구하였다.

• Journal of the Korean Applied Science and Technology
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• v.37 no.3
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• pp.571-581
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• 2020

The purpose of this study was to investigate the effect of endurance and resistance exercise by using different intensity on diabetic-related blood profiles in impaired glucose tolerance mice. 54 C57BL/6 mice were divided into (1) Control group (CO, n=9), (2) impaired glucose tolerance group (IGT, n=9), (3) IGT + 50% of VO_2max endurance exercise group (IGT50A, n=9), (4) IGT + 75% of VO_2max endurance exercise group (IGT75A, n=9), (5) IGT +50% of 1RM resistance exercise group (IGT50R, n=9), 그리고 (6) IGT + 75% of 1RM resistance exercise group (IGT75R, n=9). Endurance exercise program was performed 40 min/day, 5 days a week for 8 weeks on treadmill. Resistance exercise program was consisted in ladder-climbing 8 set/day, 5 days a week for 8 weeks. After completed the exercise program, there was no different insulin level in the groups. The fasting glucose was significantly lower in CO than in other groups, and it was not different among the exercised groups. However, IGT75R was statistically lower than IGT. HOMA-IR was only different between CO and IGT. HbA1c was higher in IGT than in other groups. As compared with exercised groups, IGT 75A was lower than IGT50R. TC was lower in CO than in other groups, but there was no different in TG. Endurance exercise groups showed higher than CO and IGT in HDL-C level. LDL-C was lower in CO than other groups. In addition, IGT75A was lower than IGT and IGT50R in LDL-C level. In conclusion, 75% of 1RM resistance exercise had more positive effect on fasting glucose, and 75% of VO_2max endurance exercise improved HbA1c and LDL-C level. In addition, endurance exercise had more effective in HDL-C improvement as compared with resistance exercise.