• Title/Summary/Keyword: 유근

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Isolation and structure elucidation of a catechin glycoside with phospholipase $A_2$ inhibiting activity from Ulmi cortex

  • Park, Sunghyouk;Goo, Yang-Mo
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1995.04a
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    • pp.58-58
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    • 1995
  • 식물에서부터 새로운 소염작용제를 개발하기 위하여 여러식물을 대상으로 염증반응의 초기단계에서 중요한 역할을 하는 것으로 알려진 Phospholipase $A_2$에 저해활성을 갖는 물질을 검색하였고 그 중 강한 억제활성을 보인 유근피에서 유효성분을 분리하였다. 유근피의 에칠 아세테이트 분획에 대하여 실리카겔 크로마토그래피, Sephadex LH-20 크로마토그래피, 분취 박막 크로마토그래피를 수행하여 phenol성 -OH기를 갖는 활성성분인 PSH-II-84-1를 분리하였다. $^1$H-NMR 신호의 양상과 짝지움 상수 값에서 분리된 물질은 (+)-catechin 의 당 유도체로 확인되었다. $^{13}$C-NMR 자료를 분석하여 치환된 당은 D-apiofuranose로 확인되었다. 방향족환의 $^{l3}$C-NMR 신호들은 extended Huekel theory를 응용하여 얻은 net charge 계산 값과 상관시켜 할당하였다. 이상의 구조연구 결과 이 물질은 (+)-catechin-7-0-$\beta$-D-apiofuranoside로 밝혀졌다. (+)-catechin-7-0-$\beta$-D-apiofuranoside의 효소억제활성은 Type II Phospholipase $A_2$에 대하여 $IC_{50}$/이 600$\mu$M이었다.다.

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Growth of and Bacterial Counts on Several Edible Sprouts Exposed to Spray Ionization (음이온 처리된 몇 가지 새싹채소의 생장과 세균 수)

  • Song, Min-Jeong;Jeon, Yu-Min;Oh, Myung-Min
    • Journal of Bio-Environment Control
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    • v.24 no.1
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    • pp.45-50
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    • 2015
  • Edible sprouts are highly nutritious, containing numerous health-promoting phytochemicals and nutrients. However, the process of sprouting is subject to potential contamination by microorganisms attached to the seed coat. The objective of this study was to determine the effects of air anions generated by spray ionization on growth and sterilization of edible sprouts. Treatment with air anions has positive effects on plant growth and sterilization in 4 kinds of sprouts. Hypocotyl length in red cabbage and kale increased approximately 1.26-fold and radicle length of lettuce, red cabbage, and kale increased 1.4 to 1.6-fold compared to the control. The fresh weight of sprouts in the spray-ionization treatment was significantly (16.0-38.5%) higher than that of the controls. Radicle activity in sprouts exposed to anions was higher than that of the control in all species. Bacterial counts on lettuce, red cabbage, and tatsoi decreased significantly (by 41%, 66%, and 19%, respectively), and bacterial colonies also declined in drainage water. Our findings suggest that spray-ionization treatment is useful for improving sprout growth and for sterilizing sprouts.

Cosmetical Properties of Polysccharides from the Root Bark of Ulmus davidiana var. Japonica (유근피로부터 추출한 다당류의 화장료적 특성)

  • Kim Young Sil;Kim Jong Heon;Kim Ki Ho
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.4 s.48
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    • pp.509-514
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    • 2004
  • The fruit or root of Ulmus davidiana var. Japonica and its extract has traditionally been used as anti inflammatation medicines as well as a remedy of the digestive ulcer for a long time. Ulmus davidiana var. Japonica has also been used to cure palliative and diuresis, because it contains lot of mucus and tannin. In this study, we examined the moisturizing effect and anti-inflammation effect of polysaccharides extracted from Ulmus davidiana var. Japonica to investigated whether it can be used as a cosmetic ingredient or not. The polysaccharides were extracted from Ulmus davidiana var. Japonica. Then the hydrolyzed was obtained through the hydrolysis. It has been ascertained that the polysaccharides we mainly composed of rhamnose, galactose, and glucose through HPLC experiments, and it has 20,000 of the molecular weight and 89.3 dL/g of intrinsic viscosity In a moisturizing test of measuring water loss in a desiccator and moisture content by Comeometer CM820, Ulmus davidiana root extract showed a similar moisturizing effect as hyaluronic acid. And its anti-inflammation effect on carrageenin-induced edema evaluated by measuring the thickness of swelling over paw induced by carrageenin (25 uL of $2\%$ carrageenin saline injection), the $\%$ swelling over paw treated with Ulmus davidiana root extract was much less than that of paw treated with carrageenin only. The anti-inflammatory effect of Ulmus davidiana root extract was almost similar to that of ketoprofen. These results can say that Ulmus davidiana root extract can be effectively used as a cosmetic ingredient.

Studies on Physiological Action of Ethylene in Rice Plant Ⅶ. Varietal Differences of Ethylene Evolution and Biosynthesis of Rice Seedling (벼에 대한 Ethylene의 생리작용에 관한 연구 제 7 보. 벼의 Ethylene 생성량 및 생합성의 품종간 차이)

  • Moon-Hee Lee;Yasuo, Ota
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.3
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    • pp.236-244
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    • 1985
  • To know the varietal differences of ethylene evolution, 55 rice varieties were tested at seedling stage. And, also, 6 rice varieties were tested the biosynthesis of ethylene production. The group of japonica varieties produced higher amount of ethylene than the indica and indica x japonica crossed varieties. The content of ACC in rice seedlings of japonica varieties was higher than in seedlings of indica and indica x japonica varieties. And, also, the conversion rate of mathionine and ACC to ethylene in seedling of japonica varieties was higher than in seedling of indica and indica x japonica varieties.

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Studies on Sheath Rot of Rice Caused by Acrocylindrium oryzae Sawada -Testing Varietal Reactions and Culture Filtrates of the Causal Fungus - (벼 잎집썩음병에 관한 연구 -품종저항성검정, 병균의 배양여액-)

  • Chung Hoo Sup
    • Korean journal of applied entomology
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    • v.14 no.1 s.22
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    • pp.23-27
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    • 1975
  • Occurrence of sheath rot rice caused by Acrocylindrium oryzae on indica varieties, IR lines and IR varieties was light to moderate in the field, whereas generally none of the recommended japonica varieties showed the disease. In general, percentage infection in the field agreed with that obtained from seed inoculation tests made in the laboratory. Culture filtrates of the fungus inhibited the sprouting of seeds of rice, barley, wheat, rye and rape. A new variety, Tongil obtained from a hybrid between japonica and indica was inhibited significantly more than was Jinheung. Conidial germination of Pyricularia oryzae was inhibited by the filtrate while no inhibitory effects were found on conidia of Cochliobolus miyabeanus.

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A Study on the Germination, Seedling Growth and Physiological Dwarfism of Apple Seed affected by $GA_3$ and Scarifications (종피파상(種皮破傷)과 $GA_3$ 처리(處理)가 Egremont russet 사과의 종자(種子) 발아(發芽), 묘(苗)의 생장(生長) 및 생리적(生理的) 왜화(矮化)에 미치는 영향)

  • Cheong, Sam Taek
    • Current Research on Agriculture and Life Sciences
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    • v.3
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    • pp.8-13
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    • 1985
  • Egremont russet 사과 품종(品種)의 종자에 여러가지 종피(種皮) 파상(破傷)을 한 후 발아(發芽), 유아(幼芽), 유근(幼根)의 생장(生長)과 생리적(生理的)인 왜화(矮花)에 대(對)하여 조사한 결과 다음과 같은 결과를 얻었다. 자엽(子葉)의 크기를 1/2로 절단하고 종피(種皮)를 제거(除去)한 Egremont russet 사과 품종(品種)의 종자(種子)에 $GA_3$를 처리한 것은 평균발아율(平均發芽率)이 55%였으며 동일조건(同一條件)에서 대조구(對照區)는 45%였다. 그리고 종피(種皮)가 완전(完全)한 것과 종피파상(種皮破傷)을 한 구(區)에서는 낮았다. 유아장(幼芽長)은 종피(種皮)를 완전히 남긴 것과 파상(破傷)한 구(區)에 $GA_3$를 처리하였을 때 촉진(促進)되었으나 전체적(全體的)으로 볼 때 종피(種皮)를 제거(除去)한 구(區)에서 더 길었다. 그러나 유근장(幼根長)은 $GA_3$처리에 의(依)해서 억제(抑制)되었으나 유근(幼根)의 직경(直徑)은 더 커졌다. 그리고 생리적(生理的)인 왜화(矮花)는 종피(種皮)를 남긴 구(區)에서 발생(發生)되었으며 $GA_3$ 처리에 의(依)해 극복되지 않았다. 자엽(子葉)의 녹색화(綠色化) 속도는 종피(種皮)를 제거(除去)하고 자엽(子葉)의 크기를 1/2로 절단한 구(區)에서 촉진(促進)되었다.

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Pathogenicity of Didymella bryoniae on the Seedlings of Cucurbits (오이류 유묘에 대한 덩굴마름병균의 병원성)

  • Lee Du Hyung
    • Korean Journal Plant Pathology
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    • v.1 no.3
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    • pp.173-177
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    • 1985
  • The objective of the study is to determine differences between cucurbits in the pathogenicity of Didymella bryoniae isolated from the naturally infected seeds of cucumber and pumpkin. Primary seedling infection of cucumber(Cucumis sativus L.), oriental melon(Cucumis melo var. makuwa Makino), pumpkin(Cucurbita pepo L.) and watermelon (Citrullus vulgaris Shrad.) occurred on the radicle, hypocotyl and cotyledons and symptoms on each crop were very similar. Infection of the radicle generally caused pre-emergence rot, while infection on the hypocotyl and cotyledons provided further inoculum for infection of the first true leaves and the stem. In cross inoculation tests, all isolates of D. bryoniae could infect cucumber, oriental melon, pumpkin and watermelon at different growth stages and there were not much differences in pathogenicity or susceptibility between isolates of the pathogen and crops tested. The susceptibility of cucumber and pumpkin was markedly influenced by prevailing humid conditions.

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Effects of Residues and Extracts of Leaf and Root Vegetables on the Germination and Growth of Cucumber and Tomato (채소류의 잔유물과 추출물이 오이와 토마토의 발아 및 초기생장에 미치는 영향)

  • Park Kuen Woo;Lee Jeong Hun;Kim Min-Jea;Won Jae Hee
    • Journal of Bio-Environment Control
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    • v.13 no.4
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    • pp.200-208
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    • 2004
  • Effects of cucumber and tomato seed germination by previous leaf and root vegetables (cabbage, radish, welsh onion, lettuce) residue in soil were tested in pot condition. Overall, suppression effect of welsh onion residue was the greatest in 4 tested crop residue and followed by radish, cabbage and lettuce, but lettuce residue didn't have effect on cucumber seed germination. Suppression were maintained ca. 20 days but after the time point, growth of cucumber and tomato were enhanced. Enhancing effect of welsh onion residue was the greatest in 4 tested crops residues and followed by radish, cabbage and lettuce. As a conclusion, residue of welsh onion, radish and cabbage were suppressed the germination of cucumber and tomato seeds but enhanced growth after 20 days of treatment. To verify the effective concentration of residue on suppression of germination and growth of radicle of cucumber and tomato, plant extract of welsh onion, radish, cabbage and lettuce were diluted as 5, 10, 17, 23, 35, 50, and $65\%$, and then tested. In low concentration treatment, 5, 10, 17, and $23\%$, all 4 crop extracts didn't suppress cucumber seed germination. High concentration of lettuce extract, 35, 50, and $65\%$, cucumber seed didn't germinate at all. In case of welsh onion extract, only $65\%$ treatment suppressed cucumber seed germination. In low concentration treatment, 17, and $23\%$, only the welsh onion extract suppressed young radicle. In case of high concentration treatment, except $35\%$, all four crops extract suppressed cucumber radicle growth. In low concentration treatment, tomato seed germination was suppressed by lettuce extract only but in high concentration treatment, 35, 50, and $65\%$, all extracts suppressed germination. Especially higher than $50\%$ treatment, tomato seed didn't germinate at all. Radicle growth was highly suppressed in welsh onion and lettuce extract, higher than $23\%$ concentration. As conclusion, leaf and root vegetable extracts suppressed cucumber and tomato seed germination and in high concentration, also suppressed radicle growth.