• Korean journal of applied entomology
• /
• v.40 no.3
• /
• pp.227-233
• /
• 2001

Four polymorphic allozymes were used for genetic analysis of overwintering populations of field diamondback moth, Plutella xylostella (Linne). Different allele frequencies were found among three local populations of Andong, Youngchon, and Yangsan in all loci. Two allozyme loci (acid phosphatase and phosphoglucomutase) showed significant deviation from Hardy-Weinberg equilibrium in allele frequencies among these populations. Estimated Nei's genetic distance varied from 0.0151 between Yangsan and Youngchon to 0.0877 between Andong and Youngchon. Compared with the previous genetic distances in this moth, a little higher genetic differentiation among these overwintering populations suggests that there would be a specific genetic bottleneck in each local population during overwintering period.

• Korean journal of applied entomology
• /
• v.54 no.4
• /
• pp.303-310
• /
• 2015

The diamondback moth, Plutella xylostella, overwinters in some protected areas in Korea. Using a sex pheromone trap, the adults were monitored since the occurrence of the overwintering populations. In Andong, P. xylostella exhibited four adult peaks in a year. Biological characters, such as cold tolerance, insecticide susceptibility, and developmental rate, were analyzed and showed a significant variation among different local overwintering populations. Population genetic variation was assessed with molecular markers, in which the initial high genetic variation among the overwintering populations decreased with the progress of seasons. These results suggests that there may be a significant migration of P. xylostella to decrease the genetic variation among the different local populations that are different in biological characters.

• Korean journal of applied entomology
• /
• v.47 no.3
• /
• pp.201-208
• /
• 2008

Spring phonology of the oriental fruit moth, Grapholita molesta, was monitored using sex pheromone traps in apple cultivating areas. Their occurrence was earlier in southern areas and their population sizes were significantly different among orchards even in a local cultivating zone. The overwintering populations appeared to move between local orchards, based on the fact that monitoring data obtained at the sites between orchards were similar to those of nearby orchards. However, within orchards, these adult movements appeared to decrease and showed skewed occurrences at the side of upwind direction or close to neighboring orchards. At initial occurrence peak(April 20-25), the ovenvintering populations of the different localities were collected and analyzed in their genetic distances. PCR-RAPD analysis indicated that there were significant genetic differences among the overwintering populations of G. molesta. This genetic differentiation of overwinterin populations may be due to genetic bottleneck following differential selection pressures against the subpopulations of G. molesta during winter on the basis of the RAPD analysis that each early spring population was significantly different to its previous fall population in the same locality.

• Korean journal of applied entomology
• /
• v.53 no.4
• /
• pp.355-365
• /
• 2014

It has been unclear whether the diamondback moth, Plutella xylostella can overwinter in Korean field conditions. This study determined overwintering conditions of P. xylostella by conducting field exposure tests based on its cold tolerance and monitoring overwintering populations by direct examination of overwintering larval habitats and capturing adults with sex pheromone traps. In addition, the overwintering populations were analyzed using polymorphic genetic markers to trace their sources. When all immature stages of P. xylostella were exposed to $-5^{\circ}C$, which was the temperature much above their supercooling points, they significantly suffered with direct cold injuries, where larval stage was most tolerant to the cold injury. However, the exposure to $5^{\circ}C$ for a long period (4 weeks) did not give any significant cold injury to nonfeeding stages, while this treatment gave lethality to larval stage without diet. When all developmental stages of P. xylostella were exposed to open field conditions during winter, they exhibited significant decreases of survival rates. However, some protected and indoor conditions reduced the cold injuries and the diet provision significantly increased larval survival rates. Adult monitoring with sex pheromone during winter period indicated that the first captures were observed at similar periods at different locations (${\approx}260$ Km apart). The overwintering adults were captured until early April. Genetic variation of these overwintering populations was analyzed with polymorphic molecular markers, indicating significant genetic divergences among the overwintering populations. This study indicates that P. xylostella can overwinter in southern Korean fields or some protected greenhouses with host plants.

• Korean journal of applied entomology
• /
• v.53 no.3
• /
• pp.287-293
• /
• 2014

Matsumuraeses phaseoli is one of the main pests that attack the flowers and pods of red bean (Vigna angularis) and mungbean (Vigna radiata) in Korea. To elucidate the developmental characteristics and over-wintering stage of M. phaseoli, several stages of the insect were observed through artificial rearing under outdoor conditions in Suwon ($37^{\circ}$16'N $126^{\circ}$59'E 35ASL), Korea. In colonies in which neonate larvae were reared at about 2-week intervals for more than a year, the developmental period from larva to adult emergence became longer from spring to summer, but shorter from summer to autumn; aestivation was not observed during the summer season. The colony in which rearing started on Oct 8, 2008 over-wintered as pupae and emerged in late April next year with a survival rate of 6%. However, the colony in which the rearing started on Oct 23, 2008 over-wintered as mature larvae, pupated in late April, and emerged in early and mid-May with a survival rate of 2%. When the fifth instar larvae were transferred outside from the laboratory ($25^{\circ}C$, 15L:9D) between November and February, no larvae could survive during the winter season. Some newly laid eggs and newly emerged adults could not hatch and could not survive, respectively, in outdoor conditions in November and December. These results suggest that neonate larvae of M. phaseoli that hatch in October can over-winter as late larvae or pupae in Suwon, Korea.

• Korean journal of applied entomology
• /
• v.45 no.2 s.143
• /
• pp.235-240
• /
• 2006

Efficacies of two commercial mating distruptor (SPLAT$^{(R)}$ and Isomate$^{(R)}$-ROSSO) were evaluated on field overwintering populations of Oriental fruit moth, Grapholita molesta (Busck), in four apple orchards. Based on the monitoring with sex pheromone traps, all the orchards (Youngchun, Kyungsan, Chungsong, and Youngju in Korea) exhibited significant overwintering populations from late April to late May. Both mating disruptors that were applied 10 days before the monitoring period significantly inhibited the male orientation to the monitoring lures, in which SPLAT type was more effective than Isomate type in the disrupting efficacy. The disruption of male orientation was highly correlated with the reduction in the early leaf damage caused mostly by G. molesta.

• Korean journal of applied entomology
• /
• v.23 no.2 s.59
• /
• pp.132-136
• /
• 1984

This study was conducted to investigate the bionomics of Curculio sikkimensis in Chonnam province from 1981 to 1983. Damage rates of the chestunt were $26.4\%$ in Damyang, $21.1\%$ in Gwangyang, and $12.9\%$ in Seungju. Adults occurred from the mid-July to the mid-September and showed peak from the mid-August to the early-September. Larvae escaped the chestnut from the early-October to the early-November. Overwintering larvae emerged $3.3\%$ in the first year, $22.78\%$ in the second year and continously overwintered $1.67\%$ in the third year. Larvae showed the highest distribution at $1.67\%$ of soil depth during the overwintering. Mortality of overwintering larvae and course of time showed the linearly correlation. Period of each stage was 5.9 days for egg and 17.17 days for pupa. Adult longevity was 9.10 days for female and 7.03 days for male.

• Research in Plant Disease
• /
• v.16 no.2
• /
• pp.109-114
• /
• 2010

The occurrence factors of Rice stripe virus (RSV) in Chungcheongbukdo were analyzed by investigating the viruliferous insect rate (VIR) of overwintered small brown plant hopper (SBPH), the population density of SBPH, the infection rate of natural host plants, and the occurrence rate of RSV on rice paddy fields at the 3 areas of Cheongwon, Jincheon, and Boeun in 2008 and 2009. The average VIR of overwintered SBPH was 0.0% in 2008 and 1.1% in 2009. From SBPH collected on early June in 2009, VIR was higher as 1.4% at Jincheon and 4.2% at Boeun than those of overwintered SBPH, and this higher VIR might relate stronlgy with the adult population of SBPH immigrated from China. The populations of SBPH at Cheongwon, Jincheon and Boeun in 2008 were 3.8, 7.5 and 20.8 Head/$m^2$, respectively. However, those of Cheongwon and Jincheon increased up to about two folds as 8.4 and 13.1 in 2009. No RSV was detected on the natural host plants including barley. The factors involved in RSV occurrence were affected negatively by the low VIR of overwintered SBPH, the low population of overwintered SBPH, the low infection rate of RSV on the natural host plants, and the clean cultivation environment in Chungcheongbukdo.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.24 no.2
• /
• pp.9-16
• /
• 1979

528 Korean land races of barley were examined for IS agronomic traits to determine if any preferential trait associations with early heading and winter hardiness existed in this collection. Eight traits were non-randomly associated with the 1977～'78 cold damage levels, and seven traits were non-randomly associated with heading date. No early heading, least-cold-damaged land race lines were found. However the least-cold-damaged class preferentially associated with mid-early heading land race lines. Ten traits were preferentially associated with the mid-early heading, least-cold-damaged class. The utilization of these associations should increase the probability that genes for winter hardiness will be preserved in the mid-early heading selections from this collection.

• Korean Journal of Environment and Ecology
• /
• v.21 no.1
• /
• pp.55-66
• /
• 2007

This study was conducted to analyze the distribution patterns of wintering waterbird communities in relation to local environmental factors in the urban streams of Seoul, Korea. A field survey was conducted at 66 sites of 5 tributaries and the main channel of the Hangang River in Seoul in January 2006. The total of 65 species and 39,560 individuals were recorded in the field survey. There were 48 species and 28,989 individuals in the Hangang River, 14 species and 1,395 individuals in the Tancheon stream, 15 species and 2,306 individuals in the Jungrangcheon stream, 22 species and 5,990 individuals in the Anyangcheon, 18 species and 283 individuals in the Changrungcheon stream, and 24 species and 597 individuals in Gokrungcheon stream. The dominant species were Anas platyrhynchos (22.65%), A. poecilorhyncha (14.01%), Aythya ferina (13.26%), Aythya fuligula (8.04%), and Mergus merganser (7.03%). Among the 16 species (with 30,650 individuals) of ducks, the dabbling and diving ducks were 8 species with 18,286 individuals and 8 species with 12,364 individuals, respectively. Through the principal component analysis, the study sites were classified into four main groups according to the similarity of their waterbirds' species compositions: 3 tributaries in the urban area (Group 1), 2 tributaries in the rural area (Group 2), one in the rural area, one in the urban area, the urban area in Hangang River (Group 3) and the main channel of the Hangang River in the urban area (Group 4). Species diversity index and species evenness were the highest in Group 1, while the lowest Group 2. Analysis on their environmental factors showed that the waterbirds wintering in urban streams of Seoul prefer broad water width, low water depth and broad resting sites.